pCMV-FLAG-CRY2PHR-FC(opt-human)-TRE3G-EGFP-Neo
$99.00
In stock
SKU
PL44915
100μg liquid plasmid(Instantly):$120
| name | pCMV-FLAG-CRY2PHR-FC(opt-human)-TRE3G-EGFP-Neo | another name | apCMV-FLAG-CRY2PHR-FC(opt-human)-TRE3G-EGFP-Neo |
| Insert Size (bp) | 2265bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATGCATTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGATTACGC CAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG GAGCTCCACC GCGGTGGCGG CCGCCACCAT GGATTACAAG GATGACGACG ATAAGGCCCG GGCGGATCCC ATGAAGATGG ACAAGAAAAC AATCGTGTGG TTTAGAAGAG ATTTGAGGAT TGAGGACAAT CCCGCTCTGG CTGCTGCCGC TCATGAAGGA AGTGTGTTTC CAGTGTTCAT CTGGTGCCCC GAGGAGGAAG GACAGTTCTA CCCTGGACGG GCTTCCAGAT GGTGGATGAA GCAGAGCCTG GCCCATCTGT CTCAGTCCCT GAAGGCTCTG GGGTCTGACC TGACTCTGAT TAAGACCCAC AACACCATCA GCGCTATCCT GGATTGTATT CGCGTGACCG GCGCCACAAA GGTGGTGTTC AACCACCTGT ACGACCCCGT GTCCCTGGTG AGAGATCACA CAGTGAAGGA AAAACTGGTG GAGAGAGGAA TCAGCGTGCA GTCCTACAAC GGCGACCTGC TGTACGAGCC TTGGGAAATC TACTGCGAGA AAGGCAAGCC CTTCACCAGC TTTAACAGCT ATTGGAAGAA GTGCCTGGAC ATGAGCATCG AGAGCGTGAT GCTGCCACCC CCTTGGAGAC TGATGCCTAT TACCGCCGCC GCTGAGGCTA TCTGGGCTTG TTCTATCGAG GAGCTGGGCC TGGAGAATGA GGCTGAAAAG CCCAGCAACG CCCTGCTGAC TAGAGCTTGG AGCCCTGGAT GGAGCAACGC TGATAAGCTG CTGAACGAGT TTATTGAGAA GCAGCTGATC GACTATGCCA AGAACAGCAA GAAGGTGGTG GGAAACTCCA CCAGCCTGCT GAGTCCTTAC CTGCACTTCG GAGAAATTAG CGTGCGGCAC GTGTTTCAGT GCGCCAGAAT GAAACAGATT ATCTGGGCCA GAGACAAAAA CAGCGAAGGC GAAGAGAGCG CCGACCTGTT TCTGAGAGGC ATCGGACTGA GGGAGTACTC TAGGTACATT TGCTTCAACT TCCCCTTCAC CCACGAGCAG AGCCTGCTGA GCCATCTGAG ATTCTTCCCC TGGGACGCCG ACGTGGATAA GTTTAAGGCC TGGAGACAGG GCCGGACCGG ATACCCTCTG GTGGATGCTG GAATGAGAGA GCTGTGGGCC ACCGGATGGA TGCATAACAG GATTAGAGTG ATTGTGAGCA GCTTCGCCGT GAAGTTCCTG CTGCTGCCTT GGAAGTGGGG CATGAAATAT TTCTGGGACA CCCTGCTGGA CGCTGACCTG GAATGTGACA TCCTGGGCTG GCAGTATATC TCTGGATCAA TCCCTGATGG ACACGAACTG GACAGACTGG ACAACCCCGC TCTGCAAGGA GCTAAATACG ACCCTGAGGG AGAGTACATC CGGCAGTGGC TGCCTGAACT GGCTAGACTG CCTACCGAGT GGATTCATCA CCCCTGGGAC GCTCCTCTGA CCGTGCTGAA GGCTTCTGGA GTGGAGCTGG GAACCAACTA TGCCAAGCCC ATCGTGGACA TCGACACCGC TAGAGAACTG CTGGCCAAGG CTATCTCTCG CACCAGAGAA GCCCAGATCA TGATCGGAGC CGCTGGAGGA GGAGGATCTG GAGGAGGAGG CTCTGGAGGA GGCGGATCTG ATAAAACACA CACATGCCCC CCATGCCCTG CTCCTGAACT GCTGGGAGGA CCTTCTGTGT TCCTGTTTCC TCCCAAGCCT AAGGACACCC TGATGATCAG CCGGACCCCT GAAGTGACCT GCGTGGTGGT GGATGTGAGC CATGAAGATC CCGAAGTGAA GTTTAACTGG TACGTGGACG GCGTGGAAGT GCACAACGCT AAGACCAAAC CCAGAGAGGA ACAGTACAAC AGTACATACA GAGTGGTGAG CGTGCTGACA GTGCTGCACC AGGATTGGCT GAACGGCAAG GAGTACAAGT GCAAGGTGAG CAACAAGGCC CTGCCCGCTC CTATTGAGAA GACCATCAGC AAAGCCAAGG GACAGCCCAG GGAGCCTCAG GTGTATACAC TGCCTCCCAG CAGAGAAGAG ATGACCAAGA ACCAGGTGAG CCTGACCTGC CTGGTGAAAG GATTCTACCC CAGCGACATC GCCGTGGAAT GGGAATCTAA TGGCCAGCCC GAGAACAACT ACAAAACAAC CCCACCCGTG CTGGACTCAG ACGGATCTTT CTTCCTGTAC AGCAAACTGA CCGTGGACAA GAGCAGGTGG CAGCAGGGAA ACGTGTTCAG CTGCTCCGTG ATGCACGAGG CTCTGCATAA CCACTATACC CAGAAGAGCC TGAGCCTGTC CCCCGGAAAA TGATGAGAAT TCGATATCAA GCTTATCGAT ACCGTCGACC TCGAGGGGGG GCCCGGTACC TTAATTAATT AAGGTACCAG GTAAGTGTAC CCAATTCGCC CTATAGTGAG TCGTATTACA ATTCACTCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG AGATCCAATT TTTAAGTGTA TAATGTGTTA AACTACTGAT TCTAATTGTT TGTGTATTTT AGATTCACAG TCCCAAGGCT CATTTCAGGC CCCTCAGTCC TCACAGTCTG TTCATGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAACGCGTA GGCCTACTAG TCCCTTTCGT CTTCACTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGAAGAGTTT ACTCCCTATC AGTGATAGAG AACGTATGCA GACTTTACTC CCTATCAGTG ATAGAGAACG TATAAGGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG ACCAGTTTAC TCCCTATCAG TGATAGAGAA CGTATCTACA GTTTACTCCC TATCAGTGAT AGAGAACGTA TATCCAGTTT ACTCCCTATC AGTGATAGAG AACGTATAAG CTATAGGCGT GTACGGTGGG CGCCTATAAA AGCAGAGCTC GTTTAGTGAA CCGTCAGATC GCCTGGAGCA ATTCCACAAC ACTTTTGTCT TATACCAACT TTCCGTACCA CTTCCTACCC TCGTAAAGGA AGCTTTCTAG AGCTAGCGTC GACACGCGTG CGGCCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTA AGGTACCACC GGTTAGTAAT GAGTTTAAAC GGGGGAGGCT AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA ACGCACGGGT GTTGGGTCGT TTGTTCAAAA TTGTAAGCGT TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAATCCTGAG GCGGAAAGAA CCAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAA CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAA CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCTAGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGTGTTGG GTCGTTTGTT CATAAACGCG GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCCTC AGGTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCC
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