pCMV-AsCpf1-HA-C-Myc-NLS-VP64-P65-Neo
$113.00
In stock
SKU
PL44897
100μg liquid plasmid(Instantly):$134
| name | pCMV-AsCpf1-HA-C-Myc-NLS-VP64-P65-Neo | another name | apCMV-AsCpf1-HA-C-Myc-NLS-VP64-P65-Neo |
| Insert Size (bp) | 4986bp | Plasmid host | Mammary cell |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC CCTCCTGGGC AACGTGCTGG TTGTTGTGCT GTCTCATCAT TTTGGCACCG GTCGCCACCA TGACACAGTT CGAGGGCTTT ACCAACCTGT ATCAGGTGAG CAAGACACTG CGGTTTGAGC TGATCCCACA GGGCAAGACC CTGAAGCACA TCCAGGAGCA GGGCTTCATC GAGGAGGACA AGGCCCGCAA TGATCACTAC AAGGAGCTGA AGCCCATCAT CGATCGGATC TACAAGACCT ATGCCGACCA GTGCCTGCAG CTGGTGCAGC TGGATTGGGA GAACCTGAGC GCCGCCATCG ACTCCTATAG AAAGGAGAAA ACCGAGGAGA CAAGGAACGC CCTGATCGAG GAGCAGGCCA CATATCGCAA TGCCATCCAC GACTACTTCA TCGGCCGGAC AGACAACCTG ACCGATGCCA TCAATAAGAG ACACGCCGAG ATCTACAAGG GCCTGTTCAA GGCCGAGCTG TTTAATGGCA AGGTGCTGAA GCAGCTGGGC ACCGTGACCA CAACCGAGCA CGAGAACGCC CTGCTGCGGA GCTTCGACAA GTTTACAACC TACTTCTCCG GCTTTTATGA GAACAGGAAG AACGTGTTCA GCGCCGAGGA TATCAGCACA GCCATCCCAC ACCGCATCGT GCAGGACAAC TTCCCCAAGT TTAAGGAGAA TTGTCACATC TTCACACGCC TGATCACCGC CGTGCCCAGC CTGCGGGAGC ACTTTGAGAA CGTGAAGAAG GCCATCGGCA TCTTCGTGAG CACCTCCATC GAGGAGGTGT TTTCCTTCCC TTTTTATAAC CAGCTGCTGA CACAGACCCA GATCGACCTG TATAACCAGC TGCTGGGAGG AATCTCTCGG GAGGCAGGCA CCGAGAAGAT CAAGGGCCTG AACGAGGTGC TGAATCTGGC CATCCAGAAG AATGATGAGA CAGCCCACAT CATCGCCTCC CTGCCACACA GATTCATCCC CCTGTTTAAG CAGATCCTGT CCGATAGGAA CACCCTGTCT TTCATCCTGG AGGAGTTTAA GAGCGACGAG GAAGTGATCC AGTCCTTCTG CAAGTACAAG ACACTGCTGA GAAACGAGAA CGTGCTGGAG ACAGCCGAGG CCCTGTTTAA CGAGCTGAAC AGCATCGACC TGACACACAT CTTCATCAGC CACAAGAAGC TGGAGACAAT CAGCAGCGCC CTGTGCGACC ACTGGGATAC ACTGAGGAAT GCCCTGTATG AGCGGAGAAT CTCCGAGCTG ACAGGCAAGA TCACCAAGTC TGCCAAGGAG AAGGTGCAGC GCAGCCTGAA GCACGAGGAT ATCAACCTGC AGGAGATCAT CTCTGCCGCA GGCAAGGAGC TGAGCGAGGC CTTCAAGCAG AAAACCAGCG AGATCCTGTC CCACGCACAC GCCGCCCTGG ATCAGCCACT GCCTACAACC CTGAAGAAGC AGGAGGAGAA GGAGATCCTG AAGTCTCAGC TGGACAGCCT GCTGGGCCTG TACCACCTGC TGGACTGGTT TGCCGTGGAT GAGTCCAACG AGGTGGACCC CGAGTTCTCT GCCCGGCTGA CCGGCATCAA GCTGGAGATG GAGCCTTCTC TGAGCTTCTA CAACAAGGCC AGAAATTATG CCACCAAGAA GCCCTACTCC GTGGAGAAGT TCAAGCTGAA CTTTCAGATG CCTACACTGG CCTCTGGCTG GGACGTGAAT AAGGAGAAGA ACAATGGCGC CATCCTGTTT GTGAAGAACG GCCTGTACTA TCTGGGCATC ATGCCAAAGC AGAAGGGCAG GTATAAGGCC CTGAGCTTCG AGCCCACAGA GAAAACCAGC GAGGGCTTTG ATAAGATGTA CTATGACTAC TTCCCTGATG CCGCCAAGAT GATCCCAAAG TGCAGCACCC AGCTGAAGGC CGTGACAGCC CACTTTCAGA CCCACACAAC CCCCATCCTG CTGTCCAACA ATTTCATCGA GCCTCTGGAG ATCACAAAGG AGATCTACGA CCTGAACAAT CCTGAGAAGG AGCCAAAGAA GTTTCAGACA GCCTACGCCA AGAAAACCGG CGACCAGAAG GGCTACAGAG AGGCCCTGTG CAAGTGGATC GACTTCACAA GGGATTTTCT GTCCAAGTAT ACCAAGACAA CCTCTATCGA TCTGTCTAGC CTGCGGCCAT CCTCTCAGTA TAAGGACCTG GGCGAGTACT ATGCCGAGCT GAATCCCCTG CTGTACCACA TCAGCTTCCA GAGAATCGCC GAGAAGGAGA TCATGGATGC CGTGGAGACA GGCAAGCTGT ACCTGTTCCA GATCTATAAC AAGGACTTTG CCAAGGGCCA CCACGGCAAG CCTAATCTGC ACACACTGTA TTGGACCGGC CTGTTTTCTC CAGAGAACCT GGCCAAGACA AGCATCAAGC TGAATGGCCA GGCCGAGCTG TTCTACCGCC CTAAGTCCAG GATGAAGAGG ATGGCACACC GGCTGGGAGA GAAGATGCTG AACAAGAAGC TGAAGGATCA GAAAACCCCA ATCCCCGACA CCCTGTACCA GGAGCTGTAC GACTATGTGA ATCACAGACT GTCCCACGAC CTGTCTGATG AGGCCAGGGC CCTGCTGCCC AACGTGATCA CCAAGGAGGT GTCTCACGAG ATCATCAAGG ATAGGCGCTT TACCAGCGAC AAGTTCTTTT TCCACGTGCC TATCACACTG AACTATCAGG CCGCCAATTC CCCATCTAAG TTCAACCAGA GGGTGAATGC CTACCTGAAG GAGCACCCCG AGACACCTAT CATCGGCATC GCCCGGGGCG AGAGAAACCT GATCTATATC ACAGTGATCG ACTCCACCGG CAAGATCCTG GAGCAGCGGA GCCTGAACAC CATCCAGCAG TTTGATTACC AGAAGAAGCT GGACAACAGG GAGAAGGAGA GGGTGGCAGC AAGGCAGGCC TGGTCTGTGG TGGGCACAAT CAAGGATCTG AAGCAGGGCT ATCTGAGCCA GGTCATCCAC GAGATCGTGG ACCTGATGAT CCACTACCAG GCCGTGGTGG TGCTGGCAAA CCTGAATTTC GGCTTTAAGA GCAAGAGGAC CGGCATCGCC GAGAAGGCCG TGTACCAGCA GTTCGAGAAG ATGCTGATCG ATAAGCTGAA TTGCCTGGTG CTGAAGGACT ATCCAGCAGA GAAAGTGGGA GGCGTGCTGA ACCCATACCA GCTGACAGAC CAGTTCACCT CCTTTGCCAA GATGGGCACC CAGTCTGGCT TCCTGTTTTA CGTGCCTGCC CCATATACAT CTAAGATCGA TCCCCTGACC GGCTTCGTGG ACCCCTTCGT GTGGAAAACC ATCAAGAATC ACGAGAGCCG CAAGCACTTC CTGGAGGGCT TCGACTTTCT GCACTACGAC GTGAAAACCG GCGACTTCAT CCTGCACTTT AAGATGAACA GAAATCTGTC CTTCCAGAGG GGCCTGCCCG GCTTTATGCC TGCATGGGAT ATCGTGTTCG AGAAGAACGA GACACAGTTT GACGCCAAGG GCACCCCTTT CATCGCCGGC AAGAGAATCG TGCCAGTGAT CGAGAATCAC AGATTCACCG GCAGATACCG GGACCTGTAT CCTGCCAACG AGCTGATCGC CCTGCTGGAG GAGAAGGGCA TCGTGTTCAG GGATGGCTCC AACATCCTGC CAAAGCTGCT GGAGAATGAC GATTCTCACG CCATCGACAC CATGGTGGCC CTGATCCGCA GCGTGCTGCA GATGCGGAAC TCCAATGCCG CCACAGGCGA GGACTATATC AACAGCCCCG TGCGCGATCT GAATGGCGTG TGCTTCGACT CCCGGTTTCA GAACCCAGAG TGGCCCATGG ACGCCGATGC CAATGGCGCC TACCACATCG CCCTGAAGGG CCAGCTGCTG CTGAATCACC TGAAGGAGAG CAAGGATCTG AAGCTGCAGA ACGGCATCTC CAATCAGGAC TGGCTGGCCT ACATCCAGGA GCTGCGCAAC GGATCCTCCG GATATCCCTA TGACGTGCCC GATTATGCCA GCCTGGGCAG CGGCTCCCCT GCTGCTAAAC GTGTTAAGCT TGATGGGGGT AGCCCGGCAG CCAAGAGAGT CAAACTCGAC GACGGCATTG GTAGTGGGAG CAACGGCAGC AGCCTCGATG CTTTAGACGA TTTTGACTTA GATATGCTTG GTTCAGACGC GTTAGACGAC TTCGACCTAG ACATGTTAGG CTCAGATGCA TTGGACGACT TCGATTTAGA TATGTTGGGC TCCGATGCCC TAGATGACTT TGATCTAGAT ATGCTAGGTA GTCCCAAAAA GAAGAGGAAA GTGGGATCCG TCCTTCCGCA AGCGCCTGCC CCAGCGCCTG CACCGGCAAT GGTGTCCGCC CTCGCACAGG CCCCTGCGCC CGTCCCCGTG CTCGCGCCTG GACCGCCCCA GGCGGTCGCT CCACCGGCTC CGAAGCCGAC GCAGGCCGGA GAGGGAACAC TCTCCGAAGC ACTTCTTCAA CTCCAGTTTG ATGACGAGGA TCTTGGAGCA CTCCTTGGAA ACTCGACAGA CCCTGCGGTG TTTACCGACC TCGCGTCAGT AGATAACTCC GAATTTCAGC AGCTTTTGAA CCAGGGTATC CCGGTCGCGC CACATACAAC GGAGCCCATG TTGATGGAAT ACCCCGAAGC AATCACGAGA CTTGTGACGG GAGCGCAGCG GCCTCCCGAT CCCGCACCCG CACCTTTGGG GGCACCTGGC CTCCCTAACG GACTTTTGAG CGGCGACGAG GATTTCTCCT CCATCGCCGA TATGGATTTC TCAGCCTTGC TGTCAGGCTC TGGCAGCGGC AGCGACCTTT CCCATCCGCC CCCAAGGGGC CATCTGGATG AGCTGACAAC CACACTTGAG TCCATGACCG AGGATCTGAA CCTGGACTCA CCCCTGACCC CGGAATTGAA CGAGATTCTG GATACCTTCC TGAACGACGA GTGCCTCTTG CATGCCATGC ATATCAGCAC AGGACTGTCC ATCTTCGACA CATCTCTGTT TGGCAGCAGC GGATCCGACG TCTGAATTCT GCAGATATCC AGCACAGTGG CGGCCGCTCG AGTCTAGAGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGT
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