pCMV-DNAH17(human)-3×FLAG-SV40-Neo
$243.00
In stock
SKU
PL44829
NM_173628.4;DNAHL1; DNEL2; SPGF39
100μg liquid plasmid(Instantly):$265
| name | pCMV-DNAH17(human)-3×FLAG-SV40-Neo | another name | apCMV-DNAH17(human)-3×FLAG-SV40-Neo |
| Insert Size (bp) | 13389bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG TACCGAGCTC GGATCCGCCA CCATGACAAT GGCCCCGGAC GTCAGACTAG AGTATCTGGA GGAAGTTGCC TCCATCGTCC TGAAGTTCAA GCCGGACAAG TGGAGCAAGC TGATAGGCGC CGAGGAGAAC GTGGCCCTGT TCACAGAGTT CTTTGAAAAG CCCGACGTCC AGGTGCTGGT GCTGACGCTC AATGCAGCCG GCATGATCAT ACCCTGCCTG GGCTTCCCCC AGTCCCTCAA GTCCAAAGGG GTTTACTTCA TCAAGACAAA GTCCGAGAAC ATCAACAAGG ACAACTACAG GGCCCGGCTC CTTTACGGCG ACATCAGCCC CACACCCGTG GACCAGCTGA TCGCGGTGGT GGAGGAGGTC CTCTCTTCTC TGTTAAACCA AAGTGAGAAC ATGGCTGGAT GGCCCCAGGT GGTCTCGGAA GACATCGTGA AGCAGGTCCA CAGGCTGAAG AATGAAATGT TTGTGATGAG TGGCAAGATC AAAGGCAAAA CCTTGCTGCC TATTCCGGAG CACCTGGGCA GCCTGGATGG CACGCTGGAG TCCATGGAGA GGATCCCCTC TTCACTGGAC AACTTGCTCC TGCACGCCAT TGAAACCACC ATCATCGACT GGTCCCACCA GATCCGGGAT GTGCTGAGCA AAGACTCAGC CCAGGCGCTG CTGGATGGGC TGCACCCCCT GCCCCAAGTG GAGTTCGAGT TCTGGGACAC TCGGCTGCTG AACCTCAAGT GCATCCATGA ACAGCTAAAC AGACCCAAAG TGAACAAGAT TGTTGAGATC CTAGAGAAAG CCAAAAGCTG CTACTGGCCA GCCCTGCAAA ACGTTTACAC CAACGTCACT GAAGGGCTGA AGGAAGCCAA CGACATCGTG CTCTATTTGA AGCCCCTACG GATCCTGCTG GAGGAGATGG AACAAGCCGA CTTCACGATG CTCCCCACCT TCATTGCCAA GGTGCTGGAC ACCATCTGCT TCATCTGGGC CACCTCTGAG TACTATAACA CACCTGCCAG GATCATCGTC ATCCTGCAGG AGTTCTGCAA CCAAATCATC GAGATGACAC GAACCTTCCT GAGCCCGGAA GAGGTGCTGA AGGGCCTGCA AGGTGAAATC GAGGAAGTCC TGAGTGGCAT CTCCCTGGCT GTAAATGTGC TGAAGGAGCT CTACCAGACG TACGACTTCT GCTGCGTGAA CATGAAGCTT TTCTTTAAGG ACAAAGAGCC CGTGCCTTGG GAATTCCCTT CTTCTCTTGC CTTTTCCAGG ATAAATTCCT TCTTCCAGCG CATCCAGACC ATTGAGGAAC TCTATAAAAC AGCAATTGAG TTTCTGAAGC TGGAGAAAAT CGAGCTTGGG GGCGTGCGTG GGAACCTCCT CGGGAGCCTG GTGACCCGTA TCTATGATGA GGTCTTTGAG CTGGTGAAGG TTTTTGCCGA CTGCAAATAT GATCCCTTGG ACCCTGGAGA CTCGAATTTT GACCGTGATT ATGCTGATTT TGAGATCAAA ATCCAAGACC TGGATAGGAG GCTGGCCACG ATCTTTTGCC AAGGATTTGA TGACTGCAGC TGTATCAAGT CCTCCGCAAA GCTCCTGTAC ATGTGTGGGG GCCTCATGGA GCGGCCCCTG ATTCTTGCCG AGGTGGCGCC CAGGTATTCA GTCATGCTGG AGCTGTTTGA CGCTGAGCTA GACAATGCTA AGATCTTGTA CGATGCCCAG ATGGCGGCCT CCGAGGAGGG GAACATCCCC CTGATCCACA AAAACATGCC TCCCGTGGCC GGGCAGCTCA AATGGAGCCT GGAGCTGCAG GAGAGGCTAG AGGTGTCCAT GAAACACCTG AAGCACGTCG AACACCCGGT CATGTCTGGA GCAGAGGCCA AGCTGACCTA TCAGAAGTAT GACGAGATGA TGGAGCTGCT GAGGTGCCAC CGCGAGAAGA TCTACCAGCA GTGGGTGGCG GGCGTGGACC AGGACTGCCA CTTTAACCTG GGGCAGCCGC TGATTCTGCG GGACGCCGCT AGCAACCTCA TCCACGTCAA CTTCAGCAAA GCGTTGGTGG CAGTTCTGAG AGAAGTCAAG TATTTGAATT TCCAGCAACA GAAAGAGATT CCAGACAGTG CGGAGAGTCT GTTCTCAGAG AACGAAACTT TCCGGAAGTT TGTGGGCAAC CTGGAGCTCA TCGTTGGCTG GTATAATGAG ATAAAGACTA TAGTGAAGGC AGTAGAATTT CTACTAATAA AGTCAGAACT GGAAGCAATT GATGTCAAGT TATTGAGCGC TGAAACGACA TTATTCTGGA ATGGCGAAGG TGTGTTTCAG TACATTCAAG AGGTGCGAGA AATTCTGCAC AACTTGCAGA ACAGGATGCA AAAGGCAAAA CAAAATATAG AAGGAATTTC CCAGGCTATG AAGGACTGGT CGGCCAACCC GCTGTTTGAA AGAAAGGACA ATAAGAAAGA GGCCCTGTTA GACTTGGATG GAAGAATTGC CAACCTCAAC AAGCGCTACG CAGCAGTCAG GGATGCTGGA GTGAAGATCC AAGCCATGGT TGCAGAAAAC GCAGAACTAT TCAGGGCAGA CACACTGAGC CTGCCCTGGA AGGATTATGT CATCTACATT GACGACATGG TCTTAGATGA ATTTGACCAG TTCATTCGCA AATCTCTGAG TTTCCTAATG GACAACATGG TTATAGATGA GAGTATCGCT CCCCTGTTTG AGATCCGCAT GGAGCTGGAC GAGGATGGGC TGACCTTCAA CCCGACCCTG GAGGTGGGCT CAGATCGCGG CTTCCTGGCA CTGATCGAGG GCCTGGTCAA CGACATCTAC AACGTAGCCA GGCTCATCCC TCGGCTGGCC AAGGACAGGA TGAACTACAA GATGGACCTG GAAGATAACA CAGACCTCAT AGAGATGAGG GAGGAGGTGT CCAGCCTGGT CATCAATGCC ATGAAGGAGG CCGAGGAGTA CCAGGATTCC TTTGAGAGGT ACTCCTACCT CTGGACGGAC AACCTGCAGG AGTTTATGAA GAATTTCCTG ATATATGGGT GTGCAGTCAC TGCGGAGGAC TTGGACACCT GGACAGATGA CACCATCCCC AAGACACCGC CCACCCTGGC TCAGTTCCAG GAGCAGATCG ACTCCTACGA GAAGCTGTAT GAGGAGGTGT CCAAGTGCGA GAACACCAAG GTGTTCCACG GCTGGCTGCA GTGCGACTGC CGCCCCTTCA AGCAGGCCCT GCTCAGCACA ATCCGGCGCT GGGGCTTCAT GTTCAAGCGG CACCTGAGCA ACCACGTCAC CAACAGCCTG GCTGACCTGG AAGCCTTCAT GAAAGTCGCC AGAATGGGCT TGACCAAGCC CCTCAAGGAG GGGGACTATG ATGGGCTTGT GGAGGTGATG GGGCACCTGA TGAAAGTCAA GGAGAGGCAA GCAGCCACCG ACAACATGTT TGAGCCCCTG AAGCAAACCA TCGAGCTGCT CAAGACCTAC GGGGAGGAGA TGCCAGAGGA GATCCACTTG AAGCTGCAGG AGCTGCCGGA GCACTGGGCA AATACCAAGA AACTGGCCAT TCAGGTGAAG CTGACCGTGG CACCACTCCA GGCCAACGAG GTCAGCATCC TGCGGCGGAA ATGCCAGCAA TTCGAGCTCA AGCAACATGA GTTCAGGGAG AGGTTCAGGC GCGAGGCCCC GTTCTCCTTC AGCGACCCCA ACCCCTACAA GTCCCTGAAT AAGCAACAAA AGAGCATCTC CGCCATGGAA GGCATCATGG AGGCGCTGTC CAAGTCCGGG GGCCTGTTCG AGGTCCCCGT CCCAGACTAC AAGCAGCTCA AGGCCTGCCA CCGGGAGGTC CGCCTACTGA AGGAGCTCTG GGACATGGTT GTTGTGGTAA ATACCAGCAT CGAGGACTGG AAGACCACCA AGTGGAAAGA TATCAACGTT GAGCAGATGG ACATAGATTG TAAGAAGTTT GCCAAGGACA TGAGGTCTTT GGACAAGGAG ATGAAAACCT GGGATGCCTT CGTGGGGCTC GACAACACCG TGAAAAACGT GATCACGTCC CTGCGTGCCG TGAGCGAGCT GCAGAACCCT GCCATTCGGG AACGCCACTG GCAGCAGCTC ATGCAGGCCA CCCAGGTGAA ATTTAAAATG TCAGAAGAGA CGACCCTGGC AGATTTACTG CAGCTGAACC TCCACAGTTA CGAGGATGAG GTCCGCAACA TCGTGGACAA GGCCGTGAAG GAGTCGGGCA TGGAAAAGGT GCTGAAAGCC CTGGACAGTA CCTGGAGCAT GATGGAATTC CAGCACGAGC CGCACCCGCG GACAGGCACC ATGATGCTCA AGTCCAGCGA GGTGCTGGTG GAGACGCTGG AGGACAACCA GGTGCAGCTG CAGAACCTGA TGATGTCCAA GTACCTGGCC CACTTCCTGA AGGAGGTGAC AAGCTGGCAG CAGAAGCTGT CCACGGCGGA CTCCGTCATC TCCATCTGGT TTGAGGTCCA GCGAACCTGG AGCCACCTGG AGAGCATCTT CATCGGCTCC GAAGACATCC GCACCCAGCT CCCGGGGGAC TCCCAGCGCT TTGACGACAT CAACCAGGAA TTCAAGGCCT TGATGGAAGA TGCAGTGAAA ACACCCAACG TGGTGGAAGC CACCAGCAAA CCCGGCCTCT ACAATAAACT GGAGGCCCTG AAGAAGAGCT TGGCCATCTG TGAAAAGGCT TTGGCAGAGT ATTTAGAGAC GAAAAGACTG GCTTTCCCCC GGTTCTATTT TGTCTCCTCG GCTGACCTCC TGGACATTCT CTCCAATGGC AATGACCCCG TGGAGGTGAG CCGCCACCTG TCCAAACTCT TCGATAGCCT GTGTAAACTG AAGTTCCGGC TCGATGCCAG TGACAAACCT CTCAAGGTGG GCCTGGGAAT GTACAGCAAG GAGGACGAGT ACATGGTTTT TGATCAGGAA TGCGACCTCT CGGGGCAGGT GGAAGTGTGG CTGAATCGAG TGCTGGACCG AATGTGCTCT ACCCTCCGGC ACGAAATCCC AGAGGCCGTG GTGACCTACG AAGAGAAGCC GAGGGAGCAG TGGATCCTGG ACTACCCAGC CCAGGTGGCC CTGACTTGCA CCCAGATCTG GTGGACGACC GAGGTGGGCC TGGCATTTGC CAGGCTGGAG GAAGGCTATG AAAACGCTAT CAGAGATTAT AACAAAAAGC AGATTAGCCA GCTGAACGTA CTCATCACGC TGCTCATGGG GAACCTCAAC GCTGGCGACA GGATGAAGAT CATGACCATC TGCACCATCG ATGTGCACGC ACGGGACGTG GTGGCCAAAA TGATCGTGGC CAAGGTGGAG AGTTCTCAGG CCTTCACCTG GCAGGCCCAG CTCCGGCATC GCTGGGACGA AGAGAAGCGA CACTGCTTTG CCAACATCTG CGATGCCCAA ATCCAGTATT CCTATGAGTA TCTGGGCAAC ACGCCGCGGC TGGTCATCAC CCCACTCACT GACAGGTGCT ATATCACCCT GACCCAGTCC CTCCATCTCA TCATGGGTGG AGCCCCTGCC GGCCCCGCTG GGACCGGCAA GACTGAGACG ACCAAGGACC TGGGCAGAGC CCTGGGCACC ATGGTCTACG TCTTCAACTG CTCCGAGCAG ATGGACTACA AGTCCTGTGG AAATATCTAC AAGGGCCTGG CCCAGACGGG AGCCTGGGGC TGCTTTGACG AGTTTAATCG CATCTCAGTG GAAGTCTTGT CTGTGATTGC CGTGCAGGTA AAATGTGTCC AGGATGCAAT TCGGGCCAAG AAAAAAGCAT TCAATTTCCT GGGAGAGATC ATAGGCCTCA TTCCCACCGT CGGTATCTTC ATCACCATGA ACCCTGGGTA CGCCGGACGC GCGGAGCTGC CTGAGAACCT AAAAGCCTTA TTCAGGCCCT GTGCCATGGT CGTCCCCGAC TTCGAACTGA TATGTGAGAT CATGCTCATG GCCGAGGGCT TTCTGGAAGC CCGCCTTCTG GCCAGGAAGT TCATCACCCT GTACACCTTG TGCAAGGAGC TGCTCTCGAA GCAGGATCAT TACGACTGGG GCCTGAGAGC CATCAAGTCT GTGCTGGTGG TGGCCGGCTC CCTGAAGAGG GGCGACCCCA GCCGGGCAGA GGACCAGGTG CTCATGCGGG CGCTGAGAGA CTTCAACATC CCCAAGATTG TGACAGACGA CCTGCCCGTA TTCATGGGAC TGATCGGGGA CCTCTTCCCG GCTCTGGACG TGCCTCGGAA ACGGGACCTG AATTTTGAAA AGATCATCAA GCAGAGCATC GTGGAGCTCA AGCTGCAGGC GGAGGACAGC TTCGTGCTGA AGGTGGTGCA GCTGGAGGAG CTGCTGCAGG TCCGCCACTC CGTGTTCATC GTCGGGAATG CGGGCAGCGG CAAATCTCAG GTCCTCAAAT CCCTCAACAA GACCTATCAG AACCTGAAGA GGAAGCCGGT CGCCGTGGAC CTGGACCCCA AGGCCGTCAC CTGCGACGAG CTCTTTGGCA TCATCAACCC AGTGACCAGG GAATGGAAAG ATGGCCTGTT CTCCACCATC ATGCGAGACC TGGCCAACAT CACCCATGAC GGCCCCAAGT GGATCATCCT TGACGGAGAC ATAGACCCCA TGTGGATCGA GTCTCTCAAC ACAGTCATGG ATGACAACAA GGTCCTCACC CTGGCCAGCA ACGAGCGGAT CCCCCTGAAC CGCACCATGA GGCTGGTGTT CGAAATCAGC CACCTGAGGA CGGCCACCCC AGCCACCGTT TCCAGAGCCG GCATCCTCTA CATCAACCCA GCCGACCTGG GATGGAACCC GGTGGTGAGC AGCTGGATCG AGAGGCGCAA GGTGCAGTCG GAGAAGGCCA ACCTGATGAT CCTCTTTGAC AAGTACCTGC CCACGTGCCT GGACAAGTTG CGCTTTGGGT TCAAGAAGAT CACGCCAGTG CCGGAGATCA CGGTGATCCA AACGATTCTG TACCTGCTGG AGTGCCTGCT CACGGAGAAG ACCGTGCCCC CCGACTCCCC CAGGGAGCTG TACGAGCTGT ACTTCGTGTT CACCTGCTTC TGGGCCTTCG GTGGCGCCAT GTTCCAGGAC CAGCTTGTGG ATTATCGAGT GGAGTTCAGT AAATGGTGGA TCAACGAATT CAAGACTATC AAGTTCCCCT CGCAGGGAAC GATTTTTGAC TACTACATTG ATCCTGACAC AAAAAAGTTC CTGCCCTGGA CAGATAAAGT GCCCTCCTTT GAGCTGGATC CCGATGTCCC ACTGCAGGCC TCTTTGGTCC ACACCACGGA AACCATCCGC ATCCGCTACT TCATGGACCT GCTCATGGAG AAGTCCTGGC CGGTGATGCT GGTGGGGAAC GCGGGGACGG GCAAGTCGGT GCTGATGGGG GACAAGCTGG AAAGCCTGAA CACGGACAAC TACCTGGTGC AGGCTGTGCC CTTCAACTTC TACACGACCT CAGCCATGCT GCAGGGGGTG CTGGAGAAGC CGCTGGAGAA GAAATCGGGG AGGAACTACG GGCCGCCAGG CACTAAGAAG CTCGTCTACT TCATCGACGA CATGAACATG CCCGAGGTGG ACAAGTATGG GACGGTGGCC CCGCACACCC TCATCCGGCA GCACATGGAC CACCGGCACT GGTATGACAG ACATAAGCTG ACGTTAAAAG ATATCCATAA TTGTCAGTAC GTGGCCTGCA TGAACCCCAC TTCCGGATCC TTCACCATCG ACTCCAGGCT TCAGCGCCAT TTCTGCGTGT TTGCTGTGAG CTTCCCCGGC CAGGAGGCCC TCACCACCAT CTACAACACA ATCCTGACGC AGCACCTGGC CTTCCGCTCG GTCTCCATGG CTATCCAGAG GATAAGCAGC CAGCTGGTGG CCGCGGCCCT GGCTTTGCAT CAGAAAATCA CGGCAACATT TCTTCCCACG GCCATTAAGT TTCATTATGT CTTCAACCTC AGGGACCTCT CCAATATTTT CCAGGGACTC TTATTTTCCA CAGCAGAAGT TCTGAAAACC CCACTGGACC TCGTCCGCCT TTGGCTACAT GAGACTGAAC GAGTGTATGG TGACAAAATG GTTGACGAAA AAGACCAGGA AACATTGCAT AGAGTCACCA TGGCCTCCAC CAAGAAGTTC TTTGATGATC TTGGTGATGA ACTCTTATTT GCCAAGCCAA ATATCTTCTG CCACTTTGCT CAAGGGATTG GCGATCCCAA ATATGTTCCT GTAACCGACA TGGCTCCTCT GAACAAGCTC CTCGTGGACG TCCTGGACAG CTACAATGAA GTTAATGCAG TCATGAATTT GGTGCTGTTT GAGGACGCCG TGGCTCACAT CTGCAGGATT AATCGCATCC TGGAGTCTCC CCGGGGGAAT GCCCTGCTGG TGGGGGTGGG CGGCAGTGGC AAACAGAGCC TCTCCCGCCT GGCAGCGTAC ATCAGCGGGC TTGACGTGTT TCAGATCACC CTCAAGAAGG GCTACGGGAT CCCCGACCTC AAGATTGACC TCGCTGCTCA GTACATAAAG GCTGCCGTGA AGAACGTTCC CTCGGTGTTC CTGATGACAG ACTCCCAGGT GGCCGAGGAG CAGTTTCTGG TGCTGATCAA TGACCTGCTG GCCTCAGGAG AGATCCCTGG GCTGTTTATG GAGGACGAGG TGGAGAACAT CATCTCCTCC ATGCGACCCC AAGTCAAGTC CCTTGGCATG AATGACACTC GGGAAACATG TTGGAAGTTC TTCATCGAAA AAGTGCGCAG ACAGCTCAAG GTGATCCTGT GTTTCTCCCC TGTGGGCTCC GTGCTGCGGG TACGAGCCAG AAAGTTCCCA GCTGTGGTCA ACTGCACGGC CATCGACTGG TTCCACGAGT GGCCGGAAGA TGCGCTGGTG TCCGTCAGCG CCCGCTTCCT GGAGGAGACT GAGGGGATTC CGTGGGAAGT CAAGGCCTCC ATCAGCTTCT TCATGTCCTA CGTGCACACC ACCGTCAACG AGATGTCCAG GGTATACCTG GCTACTGAGA GGCGCTACAA CTACACCACA CCCAAAACCT TTCTGGAGCA GATCAAACTG TACCAGAACC TGCTGGCCAA GAAGAGAACG GAACTTGTTG CCAAAATCGA GAGGCTGGAG AACGGCCTGA TGAAGCTGCA GAGCACGGCT TCCCAGGTGG ATGATTTGAA AGCCAAGTTG GCGATTCAGG AGGCTGAGCT CAAGCAGAAG AATGAGAGCG CAGACCAACT GATCCAGGTG GTCGGCATCG AGGCCGAGAA GGTCAGCAAA GAGAAGGCCA TTGCTGACCA GGAAGAAGTC AAGGTCGAGG TCATCAATAA GAACGTCACT GAGAAGCAAA AGGCCTGTGA AACAGACCTG GCCAAAGCAG AACCGGCCCT GCTGGCAGCC CAGGAGGCTC TGGACACTCT GAATAAGAAC AACCTGACAG AGCTGAAGTC CTTTGGGTCC CCGCCGGATG CTGTGGTCAA CGTCACCGCC GCCGTCATGA TTCTGACCGC ACCTGGGGGC AAGATCCCCA AGGACAAGAG CTGGAAGGCG GCCAAGATCA TGATGGGCAA GGTGGACACC TTCCTAGACT CCCTGAAGAA GTTCGACAAG GAGCACATCC CTGAGGCCTG CCTGAAGGCC TTCAAGCCCT ACCAAGGCAA CCCGACGTTC GACCCCGAGT TCATCCGCTC CAAGTCCACG GCCGCCGCCG GCCTGTGCTC CTGGTGCATC AACATCGTCC GCTTCTACGA GGTCTACTGC GACGTGGCGC CCAAGAGGCA GGCACTGGAG GAGGCTAATG CAGAGCTGGC AGAGGCACAA GAGAAGCTGT CCCGGATCAA AAACAAGATT GCCGAACTTA ACGCCAACCT GAGCAACCTA ACCTCAGCGT TTGAAAAAGC AACAGCTGAG AAAATCAAGT GTCAGCAAGA GGCCGATGCC ACGAACAGGG TGATCTTACT GGCGAACAGG CTGGTCGGGG GATTAGCATC GGAAAACATC CGCTGGGCTG AGTCTGTGGA GAACTTCAGG AGCCAGGGGG TCACGCTGTG TGGGGACGTC CTGCTCATCT CTGCCTTCGT GTCCTACGTG GGCTACTTCA CCAAGAAATA CCGGAATGAG CTGATGGAGA AATTCTGGAT CCCTTACATA CATAACTTAA AGGTCCCCAT CCCGATCACG AATGGCCTGG ATCCCTTGAG CCTGCTGACA GATGACGCGG ACGTGGCCAC CTGGAACAAC CAGGGCCTCC CCAGCGACCG CATGTCCACC GAGAATGCCA CCATCCTGGG CAACACCGAG CGGTGGCCGC TGATCGTGGA CGCCCAGCTC CAAGGAATCA AGTGGATCAA AAACAAATAC AGGAGTGAAC TGAAAGCCAT CCGCCTGGGA CAGAAGAGCT ACCTGGATGT CATCGAGCAG GCCATCTCGG AAGGGGACAC CTTGCTCATT GAGAACATCG GCGAAACCGT GGACCCCGTG CTGGACCCTC TACTGGGCAG GAACACGATT AAAAAGGGAA AGTACATTAA GATCGGTGAC AAGGAGGTGG AGTACCACCC CAAGTTCCGC CTGATCCTAC ACACCAAGTA CTTCAACCCA CACTACAAGC CAGAGATGCA GGCTCAGTGC ACCCTCATCA ACTTCCTGGT CACCAGGGAT GGACTCGAGG ACCAACTCTT GGCCGCTGTG GTGGCCAAAG AGCGCCCAGA TCTGGAACAG CTGAAGGCAA ACCTCACCAA GTCTCAAAAC GAATTTAAGA TTGTTCTGAA AGAGCTGGAA GATTCGCTCC TGGCCCGTCT GTCGGCTGCG TCGGGGAACT TTCTGGGAGA CACGGCCTTG GTGGAGAATC TGGAGACCAC CAAGCACACA GCCAGCGAGA TCGAGGAGAA GGTGGTGGAG GCAAAAATCA CAGAAGTTAA AATCAACGAA GCGAGAGAGA ACTACCGCCC GGCTGCGGAG AGGGCATCTC TGCTCTACTT CATACTGAAC GATCTCAACA AAATCAACCC CGTCTACCAG TTCTCCCTCA AGGCCTTCAA CGTGGTGTTT GAGAAAGCCA TCCAGAGGAC CACCCCTGCC AACGAGGTGA AGCAGCGGGT GATCAACCTG ACGGACGAGA TCACCTACTC CGTCTACATG TACACGGCCC GGGGACTCTT CGAGAGGGAC AAACTCATTT TCCTGGCACA AGTTACGTTT CAGGTCCTGT CCATGAAGAA GGAGCTGAAC CCAGTGGAGC TGGATTTCCT CCTGCGGTTC CCTTTTAAGG CCGGAGTGGT CTCACCAGTG GACTTCCTCC AGCATCAAGG CTGGGGCGGG ATCAAGGCCC TCTCGGAGAT GGATGAGTTC AAAAATCTGG ACAGTGACAT CGAAGGATCT GCCAAGCGCT GGAAAAAGCT GGTGGAGTCG GAAGCCCCCG AGAAGGAGAT CTTCCCCAAG GAGTGGAAGA ACAAGACGGC CCTGCAGAAG CTGTGCATGG TGCGCTGCCT GCGGCCAGAT CGCATGACCT ACGCTATCAA GAACTTCGTG GAGGAAAAGA TGGGCAGCAA GTTCGTGGAA GGCCGGAGTG TTGAGTTTTC TAAGTCCTAC GAGGAGAGCA GCCCCTCCAC GTCAATCTTC TTCATCCTCT CCCCGGGGGT TGACCCCTTG AAAGACGTGG AAGCCCTGGG AAAAAAACTA GGGTTTACCA TAGACAATGG AAAACTCCAT AATGTGTCCC TGGGGCAGGG ACAAGAGGTG GTGGCTGAGA ACGCCCTGGA CGTGGCTGCA GAGAAAGGAC ACTGGGTCAT TCTGCAGAAT ATCCACCTGG TGGCCCGGTG GCTGGGAACA CTGGACAAGA AGCTGGAGCA CTACAGCACG GGCAGCCATG AGGACTACCG GGTGTTCATC AGCGCGGAGC CTGCCCCCAG CCCCGAGACC CACATCATCC CCCAGGGCAT TCTGGAGAAC GCCATCAAGA TCACCAACGA GCCCCCCACG GGCATGCACG CCAACTTGCA CAAGGCCCTG GACCTGTTCA CCCAGGACAC CCTGGAGATG TGCACCAAGG AGATGGAGTT CAAGTGCATG CTCTTCGCCC TGTGCTACTT CCACGCTGTG GTGGCAGAGA GGCGCAAGTT CGGCGCCCAG GGCTGGAACC GGTCGTACCC CTTCAACAAC GGGGACCTCA CCATCTCCAT CAACGTGCTC TACAACTACC TGGAGGCCAA CCCCAAGGTG CCCTGGGACG ATCTCCGCTA CCTTTTTGGT GAAATCATGT ATGGCGGCCA CATCACAGAT GACTGGGACC GTCGGCTGTG CAGGACCTAC CTGGCTGAAT ACATCCGGAC GGAGATGCTG GAGGGAGACG TCCTGCTGGC CCCCGGCTTT CAGATCCCCC CCAACCTGGA CTACAAGGGT TACCACGAAT ACATCGATGA GAACCTGCCC CCTGAGAGTC CCTATCTGTA TGGCCTGCAC CCCAACGCAG AGATTGGCTT TCTGACGGTC ACCTCAGAGA AGCTGTTCCG CACTGTCCTG GAAATGCAGC CAAAAGAGAC GGACTCGGGG GCAGGCACGG GAGTGTCCCG CGAGGAGAAG GTGAAGGCCG TGCTGGACGA CATCCTGGAG AAGATTCCGG AGACTTTCAA CATGGCTGAG ATCATGGCAA AGGCAGCGGA AAAGACCCCC TACGTGGTAG TCGCCTTTCA AGAATGTGAA AGAATGAACA TCCTGACCAA CGAAATGCGC CGTTCGCTCA AGGAGCTGAA CCTGGGGCTG AAGGGAGAAC TGACCATCAC GACCGACGTG GAAGATCTGT CCACGGCTCT CTTCTATGAC ACCGTGCCTG ATACGTGGGT GGCCCGGGCC TACCCCTCCA TGATGGGCCT GGCGGCCTGG TACGCAGACC TGCTGCTCCG CATCAGGGAA CTCGAGGCCT GGACGACAGA CTTTGCCCTG CCCACCACCG TGTGGCTGGC CGGCTTCTTC AACCCCCAGT CGTTCCTCAC GGCCATCATG CAGTCCATGG CCAGGAAGAA CGAGTGGCCC CTGGACAAGA TGTGTCTGTC TGTCGAGGTG ACCAAGAAAA ACCGAGAGGA CATGACCGCT CCTCCGCGAG AGGGCTCCTA CGTGTACGGA CTCTTCATGG AAGGGGCTCG CTGGGACACC CAGACTGGAG TCATCGCTGA AGCGCGGCTG AAAGAGCTGA CCCCGGCCAT GCCTGTCATC TTCATCAAGG CCATTCCTGT GGACCGCATG GAGACCAAGA ACATCTATGA GTGTCCCGTG TACAAAACAC GCATCCGCGG CCCCACCTAT GTCTGGACCT TTAACTTGAA GACCAAAGAG AAGGCAGCGA AGTGGATCCT GGCAGCCGTG GCGCTGCTCC TACAGGTTGA ATTCTGCAGA TATCCAGCAC AGTGGCGGCC GCTCGAGGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGTGAG GGCCCGATAT CTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGA AACGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GGTGTTGGGT CGTTTGTTCA TAAACGCGGG GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA GACCCCATTG GGGCCAATAC GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT CGGGTGAAGG CCCAGGGCTC GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCAGATC TGCGCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GGGGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATAC
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