NM_080683.3;FAP-1; hPTP1E; PNP1; PTP-BAS; PTP-BL;PTP1E; PTPL1; PTPLE
100μg liquid plasmid(Instantly):$178
| name | pCMV-PTPN13(human)-3×HA-Neo | another name | apCMV-PTPN13(human)-3×HA-Neo |
| Insert Size (bp) | 7458bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC CGCCACCATG CACGTGTCAC TAGCTGAGGC CCTGGAGGTT CGGGGTGGAC CACTTCAGGA GGAAGAAATA TGGGCTGTAT TAAATCAAAG TGCTGAAAGT CTCCAAGAAT TATTCAGAAA AGTAAGCCTA GCTGATCCTG CTGCCCTTGG CTTCATCATT TCTCCATGGT CTCTGCTGTT GCTGCCATCT GGTAGTGTGT CATTTACAGA TGAAAATATT TCCAATCAGG ATCTTCGAGC ATTCACTGCA CCAGAGGTTC TTCAAAATCA GTCACTAACT TCTCTCTCAG ATGTTGAAAA GATCCACATT TATTCTCTTG GAATGACACT GTATTGGGGG GCTGATTATG AAGTGCCTCA GAGCCAACCT ATTAAGCTTG GAGATCATCT CAACAGCATA CTGCTTGGAA TGTGTGAGGA TGTTATTTAC GCTCGAGTTT CTGTTCGGAC TGTGCTGGAT GCTTGCAGTG CCCACATTAG GAATAGCAAT TGTGCACCCT CATTTTCCTA CGTGAAACAC TTGGTAAAAC TGGTTCTGGG AAATCTTTCT GGGACAGATC AGCTTTCCTG TAACAGTGAA CAAAAGCCTG ATCGAAGCCA GGCTATTCGA GATCGATTGC GAGGAAAAGG ATTACCAACA GGAAGAAGCT CTACTTCTGA TGTACTAGAC ATACAAAAGC CTCCACTCTC TCATCAGACC TTTCTTAACA AAGGGCTTAG TAAATCTATG GGATTTCTGT CCATCAAAGA TACACAAGAT GAGAATTATT TCAAGGACAT TTTATCAGAT AATTCTGGAC GTGAAGATTC TGAAAATACA TTCTCCCCTT ACCAGTTCAA AACTAGTGGC CCAGAAAAAA AACCCATCCC TGGCATTGAT GTGCTTTCTA AGAAGAAGAT CTGGGCTTCA TCCATGGACT TGCTTTGTAC AGCTGACAGA GACTTCTCTT CAGGAGAGAC TGCCACATAT CGTCGTTGTC ACCCTGAGGC AGTAACAGTG CGGACTTCAA CTACTCCTAG AAAAAAGGAG GCAAGATACT CAGATGGAAG TATAGCCTTG GATATCTTTG GCCCTCAGAA AATGGATCCA ATATATCACA CTCGAGAATT GCCCACCTCC TCAGCAATAT CAAGTGCTTT GGACCGAATC CGAGAGAGAC AAAAGAAACT TCAGGTTCTG AGGGAAGCCA TGAATGTAGA AGAACCAGTT CGAAGATACA AAACTTATCA TGGTGATGTC TTTAGTACCT CCAGTGAAAG TCCATCTATT ATTTCCTCTG AATCAGATTT CAGACAAGTG AGAAGAAGTG AAGCCTCAAA GAGGTTTGAA TCCAGCAGTG GTCTCCCAGG GGTAGATGAA ACCTTAAGTC AAGGCCAGTC ACAGAGACCG AGCAGACAAT ATGAAACACC CTTTGAAGGC AACTTAATTA ATCAAGAGAT CATGCTAAAA CGGCAAGAGG AAGAACTGAT GCAGCTACAA GCCAAAATGG CCCTTAGACA GTCTCGGTTG AGCCTATATC CAGGAGACAC AATCAAAGCG TCCATGCTTG ACATCACCAG GGATCCGTTA AGAGAAATTG CCCTAGAAAC AGCCATGACT CAAAGAAAAC TGAGGAATTT CTTTGGCCCT GAGTTTGTGA AAATGACAAT TGAACCATTT ATATCTTTGG ATTTGCCACG GTCTATTCTT ACTAAGAAAG GGAAGAATGA GGATAACCGA AGGAAAGTAA ACATAATGCT TCTGAACGGG CAAAGACTGG AACTGACCTG TGATACCAAA ACTATATGTA AAGATGTGTT TGATATGGTT GTGGCACATA TTGGCTTAGT AGAGCATCAT TTGTTTGCTT TAGCTACCCT CAAAGATAAT GAATATTTCT TTGTTGATCC TGACTTAAAA TTAACCAAAG TGGCCCCAGA GGGATGGAAA GAAGAACCAA AGAAAAAGAC CAAAGCCACT GTTAATTTTA CTTTGTTTTT CAGAATTAAA TTTTTTATGG ATGATGTTAG TCTAATACAA CATACTCTGA CGTGTCATCA GTATTACCTT CAGCTTCGAA AAGATATTTT GGAGGAAAGG ATGCACTGTG ATGATGAGAC TTCCTTATTG CTGGCATCCT TGGCTCTCCA GGCTGAGTAT GGAGATTATC AACCAGAGGT TCATGGTGTG TCTTACTTTA GAATGGAGCA CTATTTGCCC GCCAGAGTGA TGGAGAAACT TGATTTATCC TATATCAAAG AAGAGTTACC CAAATTGCAT AATACCTATG TGGGAGCTTC TGAAAAAGAG ACAGAGTTAG AATTTTTAAA GGTCTGCCAA AGACTGACAG AATATGGAGT TCATTTTCAC CGAGTGCACC CTGAGAAGAA GTCACAAACA GGAATATTGC TTGGAGTCTG TTCTAAAGGT GTCCTTGTGT TTGAAGTTCA CAATGGAGTG CGCACATTGG TCCTTCGCTT TCCATGGAGG GAAACCAAGA AAATATCTTT TTCTAAAAAG AAAATCACAT TGCAAAATAC ATCAGATGGA ATAAAACATG GCTTCCAGAC AGACAACAGT AAGATATGCC AGTACCTGCT GCACCTCTGC TCTTACCAGC ATAAGTTCCA GCTACAGATG AGAGCAAGAC AGAGCAACCA AGATGCCCAA GATATTGAGA GAGCTTCGTT TAGGAGCCTG AATCTCCAAG CAGAGTCTGT TAGAGGATTT AATATGGGAC GAGCAATCAG CACTGGCAGT CTGGCCAGCA GCACCCTCAA CAAACTTGCT GTTCGACCTT TATCAGTTCA AGCTGAGATT CTGAAGAGGC TATCCTGCTC AGAGCTGTCG CTTTACCAGC CATTGCAAAA CAGTTCAAAA GAGAAGAATG ACAAAGCTTC ATGGGAGGAA AAGCCTAGAG AGATGAGTAA ATCATACCAT GATCTCAGTC AGGCCTCTCT CTATCCACAT CGGAAAAATG TCATTGTTAA CATGGAACCC CCACCACAAA CCGTTGCAGA GTTGGTGGGA AAACCTTCTC ACCAGATGTC AAGATCTGAT GCAGAATCTT TGGCAGGAGT GACAAAACTT AATAATTCAA AGTCTGTTGC GAGTTTAAAT AGAAGTCCTG AAAGGAGGAA ACATGAATCA GACTCCTCAT CCATTGAAGA CCCTGGGCAA GCATATGTTC TAGGAATGAC TATGCATAGT TCTGGAAACT CTTCATCCCA AGTACCCTTA AAAGAAAATG ATGTGCTACA CAAAAGATGG AGCATAGTAT CTTCACCAGA AAGGGAGATC ACCTTAGTGA ACCTGAAAAA AGATGCAAAG TATGGCTTGG GATTTCAAAT TATTGGTGGG GAGAAGATGG GAAGACTGGA CCTAGGCATA TTTATCAGTT CAGTTGCCCC TGGAGGACCA GCTGACTTGG ATGGATGCTT GAAGCCAGGA GACCGTTTGA TATCTGTGAA TAGTGTGAGT CTGGAGGGAG TCAGCCACCA TGCTGCAATT GAAATTTTGC AAAATGCACC TGAAGATGTG ACACTTGTTA TCTCTCAGCC AAAAGAAAAG ATATCCAAAG TGCCTTCTAC TCCTGTGCAT CTCACCAATG AGATGAAAAA CTACATGAAG AAATCTTCCT ACATGCAAGA CAGTGCTATA GATTCTTCTT CCAAGGATCA CCACTGGTCA CGTGGTACCC TGAGGCACAT CTCGGAGAAC TCCTTTGGGC CATCTGGGGG CCTGCGGGAA GGAAGCCTGA GTTCTCAAGA TTCCAGGACT GAGAGTGCCA GCTTGTCTCA AAGCCAGGTC AATGGTTTCT TTGCCAGCCA TTTAGGTGAC CAAACCTGGC AGGAATCACA GCATGGCAGC CCTTCCCCAT CTGTAATATC CAAAGCCACC GAGAAAGAGA CTTTCACTGA TAGTAACCAA AGCAAAACTA AAAAGCCAGG CATTTCTGAT GTAACTGATT ACTCAGACCG TGGAGATTCA GACATGGATG AAGCCACTTA CTCCAGCAGT CAGGATCATC AAACACCAAA ACAGGAATCT TCCTCTTCAG TGAATACATC CAACAAGATG AATTTTAAAA CTTTTTCTTC ATCACCTCCT AAGCCTGGAG ATATCTTTGA GGTTGAACTG GCTAAAAATG ATAACAGCTT GGGGATAAGT GTCACGGGAG GTGTGAATAC GAGTGTCAGA CATGGTGGCA TTTATGTGAA AGCTGTTATT CCCCAGGGAG CAGCAGAGTC TGATGGTAGA ATTCACAAAG GTGATCGCGT CCTAGCTGTC AATGGAGTTA GTCTAGAAGG AGCCACCCAT AAGCAAGCTG TGGAAACACT GAGAAATACA GGACAGGTGG TTCATCTGTT ATTAGAAAAG GGACAATCTC CAACATCTAA AGAACATGTC CCGGTAACCC CACAGTGTAC CCTTTCAGAT CAGAATGCCC AAGGTCAAGG CCCAGAAAAA GTGAAGAAAA CAACTCAGGT CAAAGACTAC AGCTTTGTCA CTGAAGAAAA TACATTTGAG GTAAAATTAT TTAAAAATAG CTCAGGTCTA GGATTCAGTT TTTCTCGAGA AGATAATCTT ATACCGGAGC AAATTAATGC CAGCATAGTA AGGGTTAAAA AGCTCTTTCC TGGACAGCCA GCAGCAGAAA GTGGAAAAAT TGATGTAGGA GATGTTATCT TGAAAGTGAA TGGAGCCTCT TTGAAAGGAC TATCTCAGCA GGAAGTCATA TCTGCTCTCA GGGGAACTGC TCCAGAAGTA TTCTTGCTTC TCTGCAGACC TCCACCTGGT GTGCTACCGG AAATTGATAC TGCGCTTTTG ACCCCACTTC AGTCTCCAGC ACAAGTACTT CCAAACAGCA GTAAAGACTC TTCTCAGCCA TCATGTGTGG AGCAAAGCAC CAGCTCAGAT GAAAATGAAA TGTCAGACAA AAGCAAAAAA CAGTGCAAGT CCCCATCCAG AAGAGACAGT TACAGTGACA GCAGTGGGAG TGGAGAAGAT GACTTAGTGA CAGCTCCAGC AAACATATCA AATTCGACCT GGAGTTCAGC TTTGCATCAG ACTCTAAGCA ACATGGTATC ACAGGCACAG AGTCATCATG AAGCACCCAA GAGTCAAGAA GATACCATTT GTACCATGTT TTACTATCCT CAGAAAATTC CCAATAAACC AGAGTTTGAG GACAGTAATC CTTCCCCTCT ACCACCGGAT ATGGCTCCTG GGCAGAGTTA TCAACCCCAA TCAGAATCTG CTTCCTCTAG TTCGATGGAT AAGTATCATA TACATCACAT TTCTGAACCA ACTAGACAAG AAAACTGGAC ACCTTTGAAA AATGACTTGG AAAATCACCT TGAAGACTTT GAACTGGAAG TAGAACTCCT CATTACCCTA ATTAAATCAG AAAAAGGAAG CCTGGGTTTT ACAGTAACCA AAGGCAATCA GAGAATTGGT TGTTATGTTC ATGATGTCAT ACAGGATCCA GCCAAAAGTG ATGGAAGGCT AAAACCTGGG GACCGGCTCA TAAAGGTTAA TGATACAGAT GTTACTAATA TGACTCATAC AGATGCAGTT AATCTGCTCC GGGCTGCATC CAAAACAGTC AGATTAGTTA TTGGACGAGT TCTAGAATTA CCCAGAATAC CAATGTTGCC TCATTTGCTA CCGGACATAA CACTAACGTG CAACAAAGAG GAGTTGGGTT TTTCCTTATG TGGAGGTCAT GACAGCCTTT ATCAAGTGGT ATATATTAGT GATATTAATC CAAGGTCCGT CGCAGCCATT GAGGGTAATC TCCAGCTATT AGATGTCATC CATTATGTGA ACGGAGTCAG CACACAAGGA ATGACCTTGG AGGAAGTTAA CAGAGCATTA GACATGTCAC TTCCTTCATT GGTATTGAAA GCAACAAGAA ATGATCTTCC AGTGGTCCCC AGCTCAAAGA GGTCTGCTGT TTCAGCTCCA AAGTCAACCA AAGGCAATGG TTCCTACAGT GTGGGGTCTT GCAGCCAGCC TGCCCTCACT CCTAATGATT CATTCTCCAC GGTTGCTGGG GAAGAAATAA ATGAAATATC GTACCCCAAA GGAAAATGTT CTACTTATCA GATAAAGGGA TCACCAAACT TGACTCTGCC CAAAGAATCT TATATACAAG AAGATGACAT TTATGATGAT TCCCAAGAAG CTGAAGTTAT CCAGTCTCTG CTGGATGTTG TGGATGAGGA AGCCCAGAAT CTTTTAAACG AAAATAATGC AGCAGGATAC TCCTGTGGTC CAGGTACATT AAAGATGAAT GGGAAGTTAT CAGAAGAGAG AACAGAAGAT ACAGACTGCG ATGGTTCACC TTTACCTGAG TATTTTACTG AGGCCACCAA AATGAATGGC TGTGAAGAAT ATTGTGAAGA AAAAGTAAAA AGTGAAAGCT TAATTCAGAA GCCACAAGAA AAGAAGACTG ATGATGATGA AATAACATGG GGAAATGATG AGTTGCCAAT AGAGAGAACA AACCATGAAG ATTCTGATAA AGATCATTCC TTTCTGACAA ACGATGAGCT CGCTGTACTC CCTGTCGTCA AAGTGCTTCC CTCTGGTAAA TACACGGGTG CCAACTTAAA ATCAGTCATT CGAGTCCTGC GGGGTTTGCT AGATCAAGGA ATTCCTTCTA AGGAGCTGGA GAATCTTCAA GAATTAAAAC CTTTGGATCA GTGTCTAATT GGGCAAACTA AGGAAAACAG AAGGAAGAAC AGATATAAAA ATATACTTCC CTATGATGCT ACAAGAGTGC CTCTTGGAGA TGAAGGTGGC TATATCAATG CCAGCTTCAT TAAGATACCA GTTGGGAAAG AAGAGTTCGT TTACATTGCC TGCCAAGGAC CACTGCCTAC AACTGTTGGA GACTTCTGGC AGATGATTTG GGAGCAAAAA TCCACAGTGA TAGCCATGAT GACTCAAGAA GTAGAAGGAG AAAAAATCAA ATGCCAGCGC TATTGGCCCA ACATCCTAGG CAAAACAACA ATGGTCAGCA ACAGACTTCG ACTGGCTCTT GTGAGAATGC AGCAGCTGAA GGGCTTTGTG GTGAGGGCAA TGACCCTTGA AGATATTCAG ACCAGAGAGG TGCGCCATAT TTCTCATCTG AATTTCACTG CCTGGCCAGA CCATGATACA CCTTCTCAAC CAGATGATCT GCTTACTTTT ATCTCCTACA TGAGACACAT CCACAGATCA GGCCCAATCA TTACGCACTG CAGTGCTGGC ATTGGACGTT CAGGGACCCT GATTTGCATA GATGTGGTTC TGGGATTAAT CAGTCAGGAT CTTGATTTTG ACATCTCTGA TTTGGTGCGC TGCATGAGAC TACAAAGACA CGGAATGGTT CAGACAGAGG ATCAATATAT TTTCTGCTAT CAAGTCATCC TTTATGTCCT GACACGTCTT CAAGCAGAAG AAGAGCAAAA ACAGCAGCCT CAGCTTCTGA AGCTCGAGTA CCCATACGAC GTACCTGATT ATGCAACTTA TCCTTATGAT GTACCTGATT ATGCTAGCTA TCCTTATGAT GTCCCAGACT ACGCATGAGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGT
