pITGA1(human)-Fluc-TK-Rluc
$70.00
In stock
SKU
PL44630
100μg liquid plasmid(Instantly):$91
| name | pITGA1(human)-Fluc-TK-Rluc | another name | apITGA1(human)-Fluc-TK-Rluc |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Rluc,Fluc | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCAACTG GCCGGTACCT TTGGGTTTAT TGAAGTTCAG AAAATAAGCA CTGATTCTAA AATTAATTCT CTAACCATAG TTATCTCTCA GTCTCTACAT TTGTATTGAC TAGACATATA AAAGATTCAT AAATATGTAA ACATGTAAAC ATAAAATTTG TAACTTGTGA AAATTAATTG TAACAATATC ACTGTTAGTG ATGGTTATCA CCAATAATTT GTAAGTCCTA GAGGAAATAA AAATATTAAA ATTGAAATTA TATATTAGCC CCCAGAAGAA AGGTAAATTT CCCAGTAATT GTAAACTTGA ACATATTATA TGTTAACCCT ACTTTATTTC AATATTTTAC AATTTCTTTT TGTTTTTTCT AATGAAATAT ATTGCAATTT GCTAAAATGC CATATATGTA TTTGCATATG TATGTATATG CTGTACATAT ATATGTACAC ATACATACAT ACACATGATT GTGTATATAT AGACACACTA TTATATGTTT TTGAAAACTA AGCAATTTGG AGAAATATCA TTGGGAAAGT ACTTACTATG ATTTTTAAAT GTCTACGAAT TAATCAATAT CTATTGGTTA CTGTTCAAAG CACAGAGAGT CATACTGAGA GGCATAAGAC ATAACTGATA CTACCAACAG GGAAACGAGA TATACACAGG AAATGAATTC CTTGGCAGGG TATGAGAGCA TTGAACACAA ACAAAGGAAC TTAAGTAAAG TCCAATGGGA TTGAACTGTG ACCCAACATA ATACAATTTT TCTAGAGACT CATTTGTGTA AATTTGAAGA CAGAACTGAG AAATGACATT ATAAAAACTA AAAACGGGAG AACGAGATTT TTAATGTATT TACTAAATAG TGAATCAGTT CAGATACATA ATAGAAAAGG AATTTCTTGA TTAATGGGTC AACATACTGA GATCTGATGG CCTTCAACCT CCTTGGGTCT GCCCACCCCA TTTTTGACGC TCCTTAAACT TCTTCAACTT TTGTCTTCTC CAGCTTTTAG TTGCTGTGAT CCTCTATCTG GCAATGTATC CAGTGAACCC TGTGGGCTTC TTCCTTCCTG TCCCTCGTGT TACCTGTTCT GTGCTACCTG TGTTGCGTTC TGCTCAACGA ACCCTCCTTT GGGTGGCACT CTTGTCCTGC CTAGCCCTAG TTCTGCTAGA GATGCTCAAT TAGTTGTTGA GTTGAAGAAT TGAATTGAAC TGTTTCCCTC AGATTCCTCT AAACACAAAT CCACTTATTC TTTCTGTCTT TGAAATTCTC AAAACCGCTA AGACACAGCT ATTTTGAAAA TAGCCAGCAT TGGTTGAAGC TAGGGTGAAT ATGGCTTTTG ACTCAGAGAG GTTGTTCAAC TCACCAGGAA AGGGAAGCAT TGCAAGTTCC TAAGCACTTC TGGACTCTTA ATGCATTTTC ACTCGTGAAA GTTTCAAGCA AGAAAAAAAT AAAGTAGTGC CCCGAGTTCT TTATTAGATT CAATTACCAG CGTACAAACA TCCTAATTCA CTTAAACAAA ATAGCCCACA CACATTCCAT TTCCAAGGAG GCTGCTTTTC TTCCTCTCTT CCCTTCTCGC TCTCCCTCTT ACTGTTTTTC CTCCGTCTTG CTTCTCCTTT TCTCCCCTTA TCCTAGTTAT CTCAAAGAAA AGCTTATCTG CAACCACCCT TTAACCACGA ATTACCATAA ACGAAGCAAC CCCCCCACCC CCCCATAAGC GAAAGCAAAG CCCTCAGTGG AGCCTGTCGG GGTGGTGGGG ACGCCCCCTT TCGTGCAAAA CGACTTCACG GTGAATTTCA AGTGTCCGCA GGGGATGGAA GGGGGGATTC CAGATCCTCG CATTTTCACA GACGGCCTAA TCCCTCTGCG CAATGGACCC GGGAAAGCTG CCTCCCGTTA AGGGCGCCCA GGCACTGTCA AAGCGCCCTC CCTGCCCGGG CCGGGAGAAG TAAGGGGTGT GAAGGCGGAG CCACTGGGCT GGCAACACCC TCCCGAAGGT CCAGCCCTTC TCGAGGATAT CAAGATCTGG CCTCGGCGGC CAAGCTTGGC AATCCGGTAC TGTTGGTAAA GCCACCATGG AAGATGCCAA AAACATTAAG AAGGGCCCAG CGCCATTCTA CCCACTCGAA GACGGGACCG CCGGCGAGCA GCTGCACAAA GCCATGAAGC GCTACGCCCT GGTGCCCGGC ACCATCGCCT TTACCGACGC ACATATCGAG GTGGACATTA CCTACGCCGA GTACTTCGAG ATGAGCGTTC GGCTGGCAGA AGCTATGAAG CGCTATGGGC TGAATACAAA CCATCGGATC GTGGTGTGCA GCGAGAATAG CTTGCAGTTC TTCATGCCCG TGTTGGGTGC CCTGTTCATC GGTGTGGCTG TGGCCCCAGC TAACGACATC TACAACGAGC GCGAGCTGCT GAACAGCATG GGCATCAGCC AGCCCACCGT CGTATTCGTG AGCAAGAAAG GGCTGCAAAA GATCCTCAAC GTGCAAAAGA AGCTACCGAT CATACAAAAG ATCATCATCA TGGATAGCAA GACCGACTAC CAGGGCTTCC AAAGCATGTA CACCTTCGTG ACTTCCCATT TGCCACCCGG CTTCAACGAG TACGACTTCG TGCCCGAGAG CTTCGACCGG GACAAAACCA TCGCCCTGAT CATGAACAGT AGTGGCAGTA CCGGATTGCC CAAGGGCGTA GCCCTACCGC ACCGCACCGC TTGTGTCCGA TTCAGTCATG CCCGCGACCC CATCTTCGGC AACCAGATCA TCCCCGACAC CGCTATCCTC AGCGTGGTGC CATTTCACCA CGGCTTCGGC ATGTTCACCA CGCTGGGCTA CTTGATCTGC GGCTTTCGGG TCGTGCTCAT GTACCGCTTC GAGGAGGAGC TATTCTTGCG CAGCTTGCAA GACTATAAGA TTCAATCTGC CCTGCTGGTG CCCACACTAT TTAGCTTCTT CGCTAAGAGC ACTCTCATCG ACAAGTACGA CCTAAGCAAC TTGCACGAGA TCGCCAGCGG CGGGGCGCCG CTCAGCAAGG AGGTAGGTGA GGCCGTGGCC AAACGCTTCC ACCTACCAGG CATCCGCCAG GGCTACGGCC TGACAGAAAC AACCAGCGCC ATTCTGATCA CCCCCGAAGG GGACGACAAG CCTGGCGCAG TAGGCAAGGT GGTGCCCTTC TTCGAGGCTA AGGTGGTGGA CTTGGACACC GGTAAGACAC TGGGTGTGAA CCAGCGCGGC GAGCTGTGCG TCCGTGGCCC CATGATCATG AGCGGCTACG TTAACAACCC CGAGGCTACA AACGCTCTCA TCGACAAGGA CGGCTGGCTG CACAGCGGCG ACATCGCCTA CTGGGACGAG GACGAGCACT TCTTCATCGT GGACCGGCTG AAGAGCCTGA TCAAATACAA GGGCTACCAG GTAGCCCCAG CCGAACTGGA GAGCATCCTG CTGCAACACC CCAACATCTT CGACGCCGGG GTCGCCGGCC TGCCCGACGA CGATGCCGGC GAGCTGCCCG CCGCAGTCGT CGTGCTGGAA CACGGTAAAA CCATGACCGA GAAGGAGATC GTGGACTATG TGGCCAGCCA GGTTACAACC GCCAAGAAGC TGCGCGGTGG TGTTGTGTTC GTGGACGAGG TGCCTAAAGG ACTGACCGGC AAGTTGGACG CCCGCAAGAT CCGCGAGATT CTCATTAAGG CCAAGAAGGG CGGCAAGATC GCCGTGAATT CTGCTTGCAA GAACTGGTTC AGTAGCTTAA GCCACTTTGT GATCCACCTT AACAGCCACG GCTTCCCTCC CGAGGTGGAG GAGCAGGCCG CCGGCACCCT GCCCATGAGC TGCGCCCAGG AGAGCGGCAT GGATAGACAC CCTGCTGCTT GCGCCAGCGC CAGGATCAAC GTCTAACTGC AGTCTAGAGT CGGGGCGGCC GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTTAAATGA GTCTTCGGAC CTCGCGGGGG CCGCTTAAGC GGTGGTTAGG GTTTGTCTGA CGCGGGGGGA GGGGGAAGGA ACGAAACACT CTCATTCGGA GGCGGCTCGG GGTTTGGTCT TGGTGGCCAC GGGCACGCAG AAGAGCGCCG CGATCCTCTT AAGCACCCCC CCGCCCTCCG TGGAGGCGGG GGTTTGGTCG GCGGGTGGTA ACTGGCGGGC CGCTGACTCG GGCGGGTCGC GCGCCCCAGA GTGTGACCTT TTCGGTCTGC TCGCAGACCC CCGGGCGGCG CCGCCGCGGC GGCGACGGGC TCGCTGGGTC CTAGGCTCCA TGGGGACCGT ATACGTGGAC AGGCTCTGGA GCATCCGCAC GACTGCGGTG ATATTACCGG AGACCTTCTG CGGGACGAGC CGGGTCACGC GGCTGACGCG GAGCGTCCGT TGGGCGACAA ACACCAGGAC GGGGCACAGG TACACTATCT TGTCACCCGG AGGCGCGAGG GACTGCAGGA GCTTCAGGGA GTGGCGCAGC TGCTTCATCC CCGTGGCCCG TTGCTCGCGT TTGCTGGCGG TGTCCCCGGA AGAAATATAT TTGCATGTCT TTAGTTCTAT GATGACACAA ACCCCGCCCA GCGTCTTGTC ATTGGCGAAT TCGAACACGC AGATGCAGTC GGGGCGGCGC GGTCCCAGGT CCACTTCGCA TATTAAGGTG ACGCGTGTGG CCTCGAACAC CGAGCGACCC TGCAGCGACC CGCTTAAAAG CTTGATTCTT CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ACTTAATACG ACTCACTATA GGCTAGCCAC CATGACTTCG AAAGTTTATG ATCCAGAACA AAGGAAACGG ATGATAACTG GTCCGCAGTG GTGGGCCAGA TGTAAACAAA TGAATGTTCT TGATTCATTT ATTAATTATT ATGATTCAGA AAAACATGCA GAAAATGCTG TTATTTTTTT ACATGGTAAC GCGGCCTCTT CTTATTTATG GCGACATGTT GTGCCACATA TTGAGCCAGT AGCGCGGTGT ATTATACCAG ACCTTATTGG TATGGGCAAA TCAGGCAAAT CTGGTAATGG TTCTTATAGG TTACTTGATC ATTACAAATA TCTTACTGCA TGGTTTGAAC TTCTTAATTT ACCAAAGAAG ATCATTTTTG TCGGCCATGA TTGGGGTGCT TGTTTGGCAT TTCATTATAG CTATGAGCAT CAAGATAAGA TCAAAGCAAT AGTTCACGCT GAAAGTGTAG TAGATGTGAT TGAATCATGG GATGAATGGC CTGATATTGA AGAAGATATT GCGTTGATCA AATCTGAAGA AGGAGAAAAA ATGGTTTTGG AGAATAACTT CTTCGTGGAA ACCATGTTGC CATCAAAAAT CATGAGAAAG TTAGAACCAG AAGAATTTGC AGCATATCTT GAACCATTCA AAGAGAAAGG TGAAGTTCGT CGTCCAACAT TATCATGGCC TCGTGAAATC CCGTTAGTAA AAGGTGGTAA ACCTGACGTT GTACAAATTG TTAGGAATTA TAATGCTTAT CTACGTGCAA GTGATGATTT ACCAAAAATG TTTATTGAAT CGGACCCAGG ATTCTTTTCC AATGCTATTG TTGAAGGTGC CAAGAAGTTT CCTAATACTG AATTTGTCAA AGTAAAAGGT CTTCATTTTT CGCAAGAAGA TGCACCTGAT GAAATGGGAA AATATATCAA ATCGTTCGTT GAGCGAGTTC TCAAAAATGA ACAATAATTC TAGAGCGGCC GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA AAATCGATAA GGATCCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGGCTC GAC
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
