pCMV-C5(human)-3×FLAG-Neo
$128.00
In stock
SKU
PL44626
NM_001735.3;C5a; C5b; C5D; CPAMD4;ECLZB
100μg liquid plasmid(Instantly):$149
| name | pCMV-C5(human)-3×FLAG-Neo | another name | apCMV-C5(human)-3×FLAG-Neo |
| Insert Size (bp) | 5031bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCGCCACC ATGGGCCTTT TGGGAATACT TTGTTTTTTA ATCTTCCTGG GGAAAACCTG GGGACAGGAG CAAACATATG TCATTTCAGC ACCAAAAATA TTCCGTGTTG GAGCATCTGA AAATATTGTG ATTCAAGTTT ATGGATACAC TGAAGCATTT GATGCAACAA TCTCTATTAA AAGTTATCCT GATAAAAAAT TTAGTTACTC CTCAGGCCAT GTTCATTTAT CCTCAGAGAA TAAATTCCAA AACTCTGCAA TCTTAACAAT ACAACCAAAA CAATTGCCTG GAGGACAAAA CCCAGTTTCT TATGTGTATT TGGAAGTTGT ATCAAAGCAT TTTTCAAAAT CAAAAAGAAT GCCAATAACC TATGACAATG GATTTCTCTT CATTCATACA GACAAACCTG TTTATACTCC AGACCAGTCA GTAAAAGTTA GAGTTTATTC GTTGAATGAC GACTTGAAGC CAGCCAAAAG AGAAACTGTC TTAACTTTCA TAGATCCTGA AGGATCAGAA GTTGACATGG TAGAAGAAAT TGATCATATT GGAATTATCT CTTTTCCTGA CTTCAAGATT CCGTCTAATC CTAGATATGG TATGTGGACG ATCAAGGCTA AATATAAAGA GGACTTTTCA ACAACTGGAA CCGCATATTT TGAAGTTAAA GAATATGTCT TGCCACATTT TTCTGTCTCA ATCGAGCCAG AATATAATTT CATTGGTTAC AAGAACTTTA AGAATTTTGA AATTACTATA AAAGCAAGAT ATTTTTATAA TAAAGTAGTC ACTGAGGCTG ACGTTTATAT CACATTTGGA ATAAGAGAAG ACTTAAAAGA TGATCAAAAA GAAATGATGC AAACAGCAAT GCAAAACACA ATGTTGATAA ATGGAATTGC TCAAGTCACA TTTGATTCTG AAACAGCAGT CAAAGAACTG TCATACTACA GTTTAGAAGA TTTAAACAAC AAGTACCTTT ATATTGCTGT AACAGTCATA GAGTCTACAG GTGGATTTTC TGAAGAGGCA GAAATACCTG GCATCAAATA TGTCCTCTCT CCCTACAAAC TGAATTTGGT TGCTACTCCT CTTTTCCTGA AGCCTGGGAT TCCATATCCC ATCAAGGTGC AGGTTAAAGA TTCGCTTGAC CAGTTGGTAG GAGGAGTCCC AGTAACACTG AATGCACAAA CAATTGATGT AAACCAAGAG ACATCTGACT TGGATCCAAG CAAAAGTGTA ACACGTGTTG ATGATGGAGT AGCTTCCTTT GTGCTTAATC TCCCATCTGG AGTGACGGTG CTGGAGTTTA ATGTCAAAAC TGATGCTCCA GATCTTCCAG AAGAAAATCA GGCCAGGGAA GGTTACCGAG CAATAGCATA CTCATCTCTC AGCCAAAGTT ACCTTTATAT TGATTGGACT GATAACCATA AGGCTTTGCT AGTGGGAGAA CATCTGAATA TTATTGTTAC CCCCAAAAGC CCATATATTG ACAAAATAAC TCACTATAAT TACTTGATTT TATCCAAGGG CAAAATTATC CACTTTGGCA CGAGGGAGAA ATTTTCAGAT GCATCTTATC AAAGTATAAA CATTCCAGTA ACACAGAACA TGGTTCCTTC ATCCCGACTT CTGGTCTATT ACATCGTCAC AGGAGAACAG ACAGCAGAAT TAGTGTCTGA TTCAGTCTGG TTAAATATTG AAGAAAAATG TGGCAACCAG CTCCAGGTTC ATCTGTCTCC TGATGCAGAT GCATATTCTC CAGGCCAAAC TGTGTCTCTT AATATGGCAA CTGGAATGGA TTCCTGGGTG GCATTAGCAG CAGTGGACAG TGCTGTGTAT GGAGTCCAAA GAGGAGCCAA AAAGCCCTTG GAAAGAGTAT TTCAATTCTT AGAGAAGAGT GATCTGGGCT GTGGGGCAGG TGGTGGCCTC AACAATGCCA ATGTGTTCCA CCTAGCTGGA CTTACCTTCC TCACTAATGC AAATGCAGAT GACTCCCAAG AAAATGATGA ACCTTGTAAA GAAATTCTCA GGCCAAGAAG AACGCTGCAA AAGAAGATAG AAGAAATAGC TGCTAAATAT AAACATTCAG TAGTGAAGAA ATGTTGTTAC GATGGAGCCT GCGTTAATAA TGATGAAACC TGTGAGCAGC GAGCTGCACG GATTAGTTTA GGGCCAAGAT GCATCAAAGC TTTCACTGAA TGTTGTGTCG TCGCAAGCCA GCTCCGTGCT AATATCTCTC ATAAAGACAT GCAATTGGGA AGGCTACACA TGAAGACCCT GTTACCAGTA AGCAAGCCAG AAATTCGGAG TTATTTTCCA GAAAGCTGGT TGTGGGAAGT TCATCTTGTT CCCAGAAGAA AACAGTTGCA GTTTGCCCTA CCTGATTCTC TAACCACCTG GGAAATTCAA GGCGTTGGCA TTTCAAACAC TGGTATATGT GTTGCTGATA CTGTCAAGGC AAAGGTGTTC AAAGATGTCT TCCTGGAAAT GAATATACCA TATTCTGTTG TACGAGGAGA ACAGATCCAA TTGAAAGGAA CTGTTTACAA CTATAGGACT TCTGGGATGC AGTTCTGTGT TAAAATGTCT GCTGTGGAGG GAATCTGCAC TTCGGAAAGC CCAGTCATTG ATCATCAGGG CACAAAGTCC TCCAAATGTG TGCGCCAGAA AGTAGAGGGC TCCTCCAGTC ACTTGGTGAC ATTCACTGTG CTTCCTCTGG AAATTGGCCT TCACAACATC AATTTTTCAC TGGAGACTTG GTTTGGAAAA GAAATCTTAG TAAAAACATT ACGAGTGGTG CCAGAAGGTG TCAAAAGGGA AAGCTATTCT GGTGTTACTT TGGATCCTAG GGGTATTTAT GGTACCATTA GCAGACGAAA GGAGTTCCCA TACAGGATAC CCTTAGATTT GGTCCCCAAA ACAGAAATCA AAAGGATTTT GAGTGTAAAA GGACTGCTTG TAGGTGAGAT CTTGTCTGCA GTTCTAAGTC AGGAAGGCAT CAATATCCTA ACCCACCTCC CCAAAGGGAG TGCAGAGGCG GAGCTGATGA GCGTTGTCCC AGTATTCTAT GTTTTTCACT ACCTGGAAAC AGGAAATCAT TGGAACATTT TTCATTCTGA CCCATTAATT GAAAAGCAGA AACTGAAGAA AAAATTAAAA GAAGGGATGT TGAGCATTAT GTCCTACAGA AATGCTGACT ACTCTTACAG TGTGTGGAAG GGTGGAAGTG CTAGCACTTG GTTAACAGCT TTTGCTTTAA GAGTACTTGG ACAAGTAAAT AAATACGTAG AGCAGAACCA AAATTCAATT TGTAATTCTT TATTGTGGCT AGTTGAGAAT TATCAATTAG ATAATGGATC TTTCAAGGAA AATTCACAGT ATCAACCAAT AAAATTACAG GGTACCTTGC CTGTTGAAGC CCGAGAGAAC AGCTTATATC TTACAGCCTT TACTGTGATT GGAATTAGAA AGGCTTTCGA TATATGCCCC CTGGTGAAAA TCGACACAGC TCTAATTAAA GCTGACAACT TTCTGCTTGA AAATACACTG CCAGCCCAGA GCACCTTTAC ATTGGCCATT TCTGCGTATG CTCTTTCCCT GGGAGATAAA ACTCACCCAC AGTTTCGTTC AATTGTTTCA GCTTTGAAGA GAGAAGCTTT GGTTAAAGGT AATCCACCCA TTTATCGTTT TTGGAAAGAC AATCTTCAGC ATAAAGACAG CTCTGTACCT AACACTGGTA CGGCACGTAT GGTAGAAACA ACTGCCTATG CTTTACTCAC CAGTCTGAAC TTGAAAGATA TAAATTATGT TAACCCAGTC ATCAAATGGC TATCAGAAGA GCAGAGGTAT GGAGGTGGCT TTTATTCAAC CCAGGACACA ATCAATGCCA TTGAGGGCCT GACGGAATAT TCACTCCTGG TTAAACAACT CCGCTTGAGT ATGGACATCG ATGTTTCTTA CAAGCATAAA GGTGCCTTAC ATAATTATAA AATGACAGAC AAGAATTTCC TTGGGAGGCC AGTAGAGGTG CTTCTCAATG ATGACCTCAT TGTCAGTACA GGATTTGGCA GTGGCTTGGC TACAGTACAT GTAACAACTG TAGTTCACAA AACCAGTACC TCTGAGGAAG TTTGCAGCTT TTATTTGAAA ATCGATACTC AGGATATTGA AGCATCCCAC TACAGAGGCT ACGGAAACTC TGATTACAAA CGCATAGTAG CATGTGCCAG CTACAAGCCC AGCAGGGAAG AATCATCATC TGGATCCTCT CATGCGGTGA TGGACATCTC CTTGCCTACT GGAATCAGTG CAAATGAAGA AGACTTAAAA GCCCTTGTGG AAGGGGTGGA TCAACTATTC ACTGATTACC AAATCAAAGA TGGACATGTT ATTCTGCAAC TGAATTCGAT TCCCTCCAGT GATTTCCTTT GTGTACGATT CCGGATATTT GAACTCTTTG AAGTTGGGTT TCTCAGTCCT GCCACTTTCA CAGTGTACGA ATACCACAGA CCAGATAAAC AGTGTACCAT GTTTTATAGC ACTTCCAATA TCAAAATTCA GAAAGTCTGT GAAGGAGCCG CGTGCAAGTG TGTAGAAGCT GATTGTGGGC AAATGCAGGA AGAATTGGAT CTGACAATCT CTGCAGAGAC AAGAAAACAA ACAGCATGTA AACCAGAGAT TGCATATGCT TATAAAGTTA GCATCACATC CATCACTGTA GAAAATGTTT TTGTCAAGTA CAAGGCAACC CTTCTGGATA TCTACAAAAC TGGGGAAGCT GTTGCTGAGA AAGACTCTGA GATTACCTTC ATTAAAAAGG TAACCTGTAC TAACGCTGAG CTGGTAAAAG GAAGACAGTA CTTAATTATG GGTAAAGAAG CCCTCCAGAT AAAATACAAT TTCAGTTTCA GGTACATCTA CCCTTTAGAT TCCTTGACCT GGATTGAATA CTGGCCTAGA GACACAACAT GTTCATCGTG TCAAGCATTT TTAGCTAATT TAGATGAATT TGCCGAAGAT ATCTTTTTAA ATGGATGCCT CGAGGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG TAGTGAGGGC CCGTTTAAAC CCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTC
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