pLV3-H1-LRP1(human)-5UTR-shRNA1-EF1a-Puro
$55.00
In stock
SKU
PL44456
100μg liquid plasmid(Instantly):$76
| name | pLV3-H1-LRP1(human)-5UTR-shRNA1-EF1a-Puro | another name | apLV3-H1-LRP1(human)-5UTR-shRNA1-EF1a-Puro |
| Insert Size (bp) | Plasmid host | Mammary cell,Lentiviral | |
| Plasmid uses | Interfere with silence | Fragment type | shRNA |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | H1 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCAGCTCCAG CTTTTGTTCC CTTTAGTGAG GGTTAATTGC GCGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCGTAATACG ACTCACTATA GGGCGAATTG GGTACGTCCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGATA GCTAGAGCCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTC CTCTCACTCT CTGATATTCA TTTCTTTGCA AGTTATAAAT ACTGAATAAT AAGATGACAT GAACTACTAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAAGC AAAAAGCAGA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG GTACCGAGCT CGAATTCCAG GCGGGGAGGC GGCCCAAAGG GAGATCCGAC TCGTCTGAGG GCGAAGGCGA AGACGCGGAA GAGGCCGCAG AGCCGGCAGC AGGCCGCGGG AAGGAAGGTC CGCTGGATTG AGGGCCGAAG GGACGTAGCA GAAGGACGTC CCGCGCAGAA TCCAGGTGGC AACACAGGCG AGCAGCCATG GAAAGGACGT CAGCTTCCCC GACAACACCA CGGAATTGTC AGTGCCCAAC AGCCGAGCCC CTGTCCAGCA GCGGGCAAGG CAGGCGGCGA TGAGTTCCGC CGTGGCAATA GGGAGGGGGA AAGCGAAAGT CCCGGAAAGG AGCTGACAGG TGGTGGCAAT GCCCCAACCA GTGGGGGTTG CGTCAGCAAA CACAGTGCAC ACCACGCCAC GTTGCCTGAC AACGGGCCAC AACTCCTCAT AAAGAGACAG CAACCAGGAT TTATACAAGG AGGAGAAAAT GAAAGCCATA CGGGAAGCAA TAGCATGATA CAAAGGCATT AAAGCAGCGT ATCCACATAG CGTAAAAGGA GCAACATAGT TAAGAATACC AGTCAATCTT TCACAAATTT TGTAATCCAG AGGTTGATTG TCGACGCGGC CGCTCAGGCA CCGGGCTTGC GGGTCATGCA CCAGGTGCGC GGTCCTTCGG GCACCTCGAC GTCGGCGGTG ACGGTGAAGC CGAGCCGCTC GTAGAAGGGG AGGTTGCGGG GCGCGGAGGT CTCCAGGAAG GCGGGCACCC CGGCGCGCTC GGCCGCCTCC ACTCCGGGGA GCACGACGGC GCTGCCCAGA CCCTTGCCCT GGTGGTCGGG CGAAACACCG ACGGTGGCCA GGAACCACGC GGGCTCCTTG GGCCGGTGCG GCGCCAGGAG GCCTTCCATC TGTTGCTGCG CGGCCAGCCG GGAACCGCTC AACTCGGCCA TGCGCGGGCC GATCTCGGCG AACACCGCCC CCGCTTCGAC GCTCTCCGGC GTGGTCCAGA CCGCCACCGC GGCGCCGTCG TCCGCGACCC ACACCTTGCC GATGTCGAGC CCGACGCGCG TGAGGAAGAG TTCTTGCAGC TCGGTGACCC GCTCGATGTG GCGGTCCGGA TCGACGGTGT GGCGCGTGGC GGGGTAGTCG GCGAACGCGG CGGCGAGGGT GCGTACGGCC CTGGGGACGT CGTCGCGGGT GGCGAGGCGC ACCGTGGGCT TGTACTCGGT CATGGTGGCG ACCGGTGGAT CTCTAGAGGG GTAGTTTTCA CGACACCTGA AATGGAAGAA AAAAACTTTG AACCACTGTC TGAGGCTTGA GAATGAACCA AGATCCAAAC TCAAAAAGGG CAAATTCCAA GGAGAATTAC ATCAAGTGCC AAGCTGGCCT AACTTCAGTC TCCACCCACT CAGTGTGGGG AAACTCCATC GCATAAAACC CCTCCCCCCA ACCTAAAGAC GACGTACTCC AAAAGCTCGA GAACTAATCG AGGTGCCTGG ACGGCGCCCG GTACTCCGTG GAGTCACATG AAGCGACGGC TGAGGACGGA AAGGCCCTTT TCCTTTGTGT GGGTGACTCA CCCGCCCGCT CTCCCGAGCG CCGCGTCCTC CATTTTGAGC TCCCTGCAGC AGGGCCGGGA AGCGGCCATC TTTCCGCTCA CGCAACTGGT GCCGACCGGG CCAGCCTTGC CGCCCAGGGC GGGGCGATAC ACGGCGGCGC GAGGCCAGGC ACCAGAGCAG GCCGGCCAGC TTGAGACTAC CCCCGTCCGA TTCTCGGTGG CCGCGCTCGC AGGCCCCGCC TCGCCGAACA TGTGCGCTGG GACGCACGGG CCCCGTCGCC GCCCGCGGCC CCAAAAACCG AAATACCAGT GTGCAGATCT TGGCCCGCAT TTACAAGACT ATCTTGCCAG AAAAAAAGCG TCGCAGGATA TCATCAAAAA TTTTAAATGG CTAGAGACTT ATCGAAAGCA GCGAGACAGG CGCGAAGGTG CCACCAGATT CGCACGCGGC GGCCCCAGCG CCCAGGCCAG GCCTCAACTC AAGCACGAGG CGAAGGGGCT CCTTAAGCGC AAGGCCTCGA ACTCTCCCAC CCACTTCCAA CCCGAAGCTC GGGATCAAGA ATCACGTACT GCAGCCAGGG GCGTGGAAGT AATTCAAGGC ACGCAAGGGC CATAACCCGT AAAGAGGCCA GGCCCGCGGG AACCACACAC GGCACTTACC TGTGTTCTGG CGGCAAACCC GTTGCGAAAA AGAACGTTCA CGGCGACTAC TGCACTTATA TACGGTTCTC CCCCACCCTC GGGAAAAAGG CGGAGCCAGT ACACGACATC ACTTTCCCAG TTTACCCCGC GCCACCTTCT CTAGGCACCG GTTCAATTGC CGACCCCTCC CCCCAACTTC TCGGGGACTG TGGGCGATGT GCGCTCTGCC CACTGACGGG CACCGGAGCC TCACGCATGC TCTTCTCCAC CTCAGTGATG ACGAGAGCGG GCGGGTGAGG GGGCGGGAAC GCAGCGATCT CTGGGTTCTA CGTTAGTGGG AGTTTAACGA CGGTCCCTGG GATTCCCCAA GGCAGGGGCG AGTCCTTTTG TATGAATTAC TCTCAGCTCC GGTCGGGGCG GGTTGGGGGG GGTGGTGACG GGGAGGCATC GATAAAATTT TGAATTTTGT AATTTGTTTT TGTAATTCTT TAGTTTGTAT GTCTGTTGCT ATTATGTCTA CTATTCTTTC CCCTGCACTG TACCCCCCAA TCCCCCCTTT TCTTTTAAAA GTTGGATCCG GCCGAATTCG AACGCTGACG TCATCAACCC GCTCCAAGGA ATCGCGGGCC CAGTGTCACT AGGCGGGAAC ACCCAGCGCG CGTGCGCCCT GGCAGGAAGA TGGCTGTGAG GGACAGGGGA GTGGCGCCCT GCAATATTTG CATGTCGCTA TGTGTTCTGG GAAATCACCA TAAACGTGAA ATGTCTTTGG ATTTGGGAAT CTTATAAGTT CTGTATGAGA CCACTCTAAA AAGCTTTGCC GAAGGAAAGA ACTCGAGTTC TTTCCTTCGG CAAAGCTTTT TTTTACTAGT GTTAACCGAT ACCGTCGAGA TCCGTTCACT AATCGAATGG ATCTGTCTCT GTCTCTCTCT CCACCTTCTT CTTCTATTCC TTCGGGCCTG TCGGGTCCCC TCGGGGTTGG GAGGTGGGTC TGAAACGATA ATGGTGAATA TCCCTGCCTA ACTCTATTCA CTATAGAAAG TACAGCAAAA ACTATTCTTA AACCTACCAA GCCTCCTACT ATCATTATGA ATAATTTTAT ATACCACAGC CAATTTGTTA TGTTAAACCA ATTCCACAAA CTTGCCCATT TATCTAATTC CAATAATTCT TGTTCATTCT TTTCTTGCTG GTTTTGCGAT TCTTCAATTA AGGAGTGTAT TAAGCTTGTG TAATTGTTAA TTTCTCTGTC CCACTCCATC CAGGTCGTGT GATTCCAAAT CTGTTCCAGA GATTTATTAC TCCAACTAGC ATTCCAAGGC ACAGCAGTGG TGCAAATGAG TTTTCCAGAG CAACCCCAAA TCCCCAGGAG CTGTTGATCC TTTAGGTATC TTTCCACAGC CAGGATTCTT GCCTGGAGCT GCTTGATGCC CCAGACTGTG AGTTGCAACA GATGCTGTTG CGCCTCAATA GCCCTCAGCA AATTGTTCTG CTGCTGCACT ATACCAGACA ATAATTGTCT GGCCTGTACC GTCAGCGTCA TTGACGCTGC GCCCATAGTG CTTCCTGCTG CTCCCAAGAA CCCAAGGAAC AAAGCTCCTA TTCCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGCTTGCT GTGCGGTGGT CTTACTTTTG TTTTGCTCTT CCTCTATCTT GTCTAAAGCT TCCTTGGTGT CTTTTATCTC TATCCTTTGA TGCACACAAT AGAGGGTTGC TACTGTATTA TATAATGATC TAAGTTCTTC TGATCCTGTC TGAAGGGATG GTTGTAGCTG TCCCAGTATT TGTCTACAGC CTTCTGATGT TTCTAACAGG CCAGGATTAA CTGCGAATCG TTCTAGCTCC CTGCTTGCCC ATACTATATG TTTTAATTTA TATTTTTTCT TTCCCCCTGG CCTTAACCGA ATTTTTTCCC ATCGCGATCT AATTCTCCCC CGCTTAATAC TGACGCTCTC GCACCCATCT CTCTCCTTCT AGCCTCCGCT AGTCAAAATT TTTGGCGTAC TCACCAGTCG CCGCCCCTCG CCTCTTGCCG TGCGCGCTTC AGCAAGCCGA GTCCTGCGTC GAGAGAGCTC CTCTGGTTTC CCTTTCGCTT TCAAGTCCCT GTTCGGGCGC CACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCGTTTAT GTATCGAGCT AGGCACTTAA ATACAATATC TCTGCAATGC GGCAATTCAG TGGTTCGTCC AATCCATGTC AGACCCGTCT GTTGCCTTCC TAATAAGGCA CGATCGTACC ACCTTACTTC CACCAATCGG CATGCACGGT GCTTTTTCTC TCCTTGTAAG GCATGTTGCT AACTCATCGT TACCATGTTG CAAGACTACA AGAGTATTGC ATAAGACTAC ATTAAGCTT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
