pCMV-LRP4(human)-3×FLAG-Neo
NM_002334.4;CLSS; CMS17; LRP-4; LRP10; MEGF7; SOST2
100μg liquid plasmid(Instantly):$149
NM_002334.4;CLSS; CMS17; LRP-4; LRP10; MEGF7; SOST2
100μg liquid plasmid(Instantly):$149
| name | pCMV-LRP4(human)-3×FLAG-Neo | another name | apCMV-LRP4(human)-3×FLAG-Neo |
| Insert Size (bp) | 5718bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F:CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCGCCACC ATGAGGCGGC AGTGGGGCGC GCTGCTGCTT GGCGCCCTGC TCTGCGCACA CGGCCTGGCC AGCAGCCCCG AGTGTGCTTG TGGTCGGAGC CACTTCACAT GTGCAGTGAG TGCTCTTGGA GAGTGTACCT GCATCCCTGC CCAGTGGCAG TGTGATGGAG ACAATGACTG CGGGGACCAC AGCGATGAGG ATGGATGTAT ACTACCTACC TGTTCCCCTC TTGACTTTCA CTGTGACAAT GGCAAGTGCA TCCGCCGCTC CTGGGTGTGT GACGGGGACA ACGACTGTGA GGATGACTCG GATGAGCAGG ACTGTCCCCC CCGGGAGTGT GAGGAGGACG AGTTTCCCTG CCAGAATGGC TACTGCATCC GGAGTCTGTG GCACTGCGAT GGTGACAATG ACTGTGGCGA CAACAGCGAT GAGCAGTGTG ACATGCGCAA GTGCTCCGAC AAGGAGTTCC GCTGTAGTGA CGGAAGCTGC ATTGCTGAGC ATTGGTACTG CGACGGTGAC ACCGACTGCA AAGATGGCTC CGATGAGGAG AACTGTCCCT CAGCAGTGCC AGCGCCCCCC TGCAACCTGG AGGAGTTCCA GTGTGCCTAT GGACGCTGCA TCCTCGACAT CTACCACTGC GATGGCGACG ATGACTGTGG AGACTGGTCA GACGAGTCTG ACTGCTCCTC CCACCAGCCC TGCCGCTCTG GGGAGTTCAT GTGTGACAGT GGCCTGTGCA TCAATGCAGG CTGGCGCTGC GATGGTGACG CGGACTGTGA TGACCAGTCT GATGAGCGCA ACTGCACCAC CTCCATGTGT ACGGCAGAAC AGTTCCGCTG TCACTCAGGC CGCTGTGTCC GCCTGTCCTG GCGCTGTGAT GGGGAGGACG ACTGTGCAGA CAACAGCGAT GAAGAGAACT GTGAGAATAC AGGAAGCCCC CAATGTGCCT TGGACCAGTT CCTGTGTTGG AATGGGCGCT GCATTGGGCA GAGGAAGCTG TGCAACGGGG TCAACGACTG TGGTGACAAC AGCGACGAAA GCCCACAGCA GAATTGCCGG CCCCGGACGG GTGAGGAGAA CTGCAATGTT AACAACGGTG GCTGTGCCCA GAAGTGCCAG ATGGTGCGGG GGGCAGTGCA GTGTACCTGC CACACAGGCT ACCGGCTCAC AGAGGATGGG CACACGTGCC AAGATGTGAA TGAATGTGCC GAGGAGGGGT ATTGCAGCCA GGGCTGCACC AACAGCGAAG GGGCTTTCCA ATGCTGGTGT GAAACAGGCT ATGAACTACG GCCCGACCGG CGCAGCTGCA AGGCTCTGGG GCCAGAGCCT GTGCTGCTGT TCGCCAATCG CATCGACATC CGGCAGGTGC TGCCACACCG CTCTGAGTAC ACACTGCTGC TTAACAACCT GGAGAATGCC ATTGCCCTTG ATTTCCACCA CCGCCGCGAG CTTGTCTTCT GGTCAGATGT CACCCTGGAC CGGATCCTCC GTGCCAACCT CAACGGCAGC AACGTGGAGG AGGTTGTGTC TACTGGGCTG GAGAGCCCAG GGGGCCTGGC TGTGGATTGG GTCCATGACA AACTCTACTG GACCGACTCA GGCACCTCGA GGATTGAGGT GGCCAATCTG GATGGGGCCC ACCGGAAAGT GTTGCTGTGG CAGAACCTGG AGAAGCCCCG GGCCATTGCC TTGCATCCCA TGGAGGGTAC CATTTACTGG ACAGACTGGG GCAACACCCC CCGTATTGAG GCCTCCAGCA TGGATGGCTC TGGACGCCGC ATCATTGCCG ATACCCATCT CTTCTGGCCC AATGGCCTCA CCATCGACTA TGCCGGGCGC CGTATGTACT GGGTGGATGC TAAGCACCAT GTCATCGAGA GGGCCAATCT GGATGGGAGT CACCGTAAGG CTGTCATTAG CCAGGGCCTC CCGCATCCCT TCGCCATCAC AGTGTTTGAA GACAGCCTGT ACTGGACAGA CTGGCACACC AAGAGCATCA ATAGCGCTAA CAAATTTACG GGGAAGAACC AGGAAATCAT TCGCAACAAA CTCCACTTCC CTATGGACAT CCACACCTTG CACCCCCAGC GCCAACCTGC AGGGAAAAAC CGCTGTGGGG ACAACAACGG AGGCTGCACG CACCTGTGTC TGCCCAGTGG CCAGAACTAC ACCTGTGCCT GCCCCACTGG CTTCCGCAAG ATCAGCAGCC ACGCCTGTGC CCAGAGTCTT GACAAGTTCC TGCTTTTTGC CCGAAGGATG GACATCCGTC GAATCAGCTT TGACACAGAG GACCTGTCTG ATGATGTCAT CCCACTGGCT GACGTGCGCA GTGCTGTGGC CCTTGACTGG GACTCCCGGG ATGACCACGT GTACTGGACA GATGTCAGCA CTGATACCAT CAGCAGGGCC AAGTGGGATG GAACAGGACA GGAGGTGGTA GTGGATACCA GTTTGGAGAG CCCAGCTGGC CTGGCCATTG ATTGGGTCAC CAACAAACTG TACTGGACAG ATGCAGGTAC AGACCGGATT GAAGTAGCCA ACACAGATGG CAGCATGAGA ACAGTACTCA TCTGGGAGAA CCTTGATCGT CCTCGGGACA TCGTGGTGGA ACCCATGGGC GGGTACATGT ATTGGACTGA CTGGGGTGCG AGCCCCAAGA TTGAACGAGC TGGCATGGAT GCCTCAGGCC GCCAAGTCAT TATCTCTTCT AATCTGACCT GGCCTAATGG GTTAGCTATT GATTATGGGT CCCAGCGTCT ATACTGGGCT GACGCCGGCA TGAAGACAAT TGAATTTGCT GGACTGGATG GCAGTAAGAG GAAGGTGCTG ATTGGAAGCC AGCTCCCCCA CCCATTTGGG CTGACCCTCT ATGGAGAGCG CATCTATTGG ACTGACTGGC AGACCAAGAG CATACAGAGC GCTGACCGGC TGACAGGGCT GGACCGGGAG ACTCTGCAGG AGAACCTGGA AAACCTAATG GACATCCATG TCTTCCACCG CCGCCGGCCC CCAGTGTCTA CACCATGTGC TATGGAGAAT GGCGGCTGTA GCCACCTGTG TCTTAGGTCC CCAAATCCAA GCGGATTCAG CTGTACCTGC CCCACAGGCA TCAACCTGCT GTCTGATGGC AAGACCTGCT CACCAGGCAT GAACAGTTTC CTCATCTTCG CCAGGAGGAT AGACATTCGC ATGGTCTCCC TGGACATCCC TTATTTTGCT GATGTGGTGG TACCAATCAA CATTACCATG AAGAACACCA TTGCCATTGG AGTAGACCCC CAGGAAGGAA AGGTGTACTG GTCTGACAGC ACACTGCACA GGATCAGTCG TGCCAATCTG GATGGCTCAC AGCATGAGGA CATCATCACC ACAGGGCTAC AGACCACAGA TGGGCTCGCG GTTGATGCCA TTGGCCGGAA AGTATACTGG ACAGACACGG GAACAAACCG GATTGAAGTG GGCAACCTGG ACGGGTCCAT GCGGAAAGTG TTGGTGTGGC AGAACCTTGA CAGTCCCCGG GCCATCGTAC TGTACCATGA GATGGGGTTT ATGTACTGGA CAGACTGGGG GGAGAATGCC AAGTTAGAGC GGTCCGGAAT GGATGGCTCA GACCGCGCGG TGCTCATCAA CAACAACCTA GGATGGCCCA ATGGACTGAC TGTGGACAAG GCCAGCTCCC AACTGCTATG GGCCGATGCC CACACCGAGC GAATTGAGGC TGCTGACCTG AATGGTGCCA ATCGGCATAC ATTGGTGTCA CCGGTGCAGC ACCCATATGG CCTCACCCTG CTCGACTCCT ATATCTACTG GACTGACTGG CAGACTCGGA GCATCCACCG TGCTGACAAG GGTACTGGCA GCAATGTCAT CCTCGTGAGG TCCAACCTGC CAGGCCTCAT GGACATGCAG GCTGTGGACC GGGCACAGCC ACTAGGTTTT AACAAGTGCG GCTCGAGAAA TGGCGGCTGC TCCCACCTCT GCTTGCCTCG GCCTTCTGGC TTCTCCTGTG CCTGCCCCAC TGGCATCCAG CTGAAGGGAG ATGGGAAGAC CTGTGATCCC TCTCCTGAGA CCTACCTGCT CTTCTCCAGC CGTGGCTCCA TCCGGCGTAT CTCACTGGAC ACCAGTGACC ACACCGATGT GCATGTCCCT GTTCCTGAGC TCAACAATGT CATCTCCCTG GACTATGACA GCGTGGATGG AAAGGTCTAT TACACAGATG TGTTCCTGGA TGTTATCAGG CGAGCAGACC TGAACGGCAG CAACATGGAG ACAGTGATCG GGCGAGGGCT GAAGACCACT GACGGGCTGG CAGTGGACTG GGTGGCCAGG AACCTGTACT GGACAGACAC AGGTCGAAAT ACCATTGAGG CGTCCAGGCT GGATGGTTCC TGCCGCAAAG TACTGATCAA CAATAGCCTG GATGAGCCCC GGGCCATTGC TGTTTTCCCC AGGAAGGGGT ACCTCTTCTG GACAGACTGG GGCCACATTG CCAAGATCGA ACGGGCAAAC TTGGATGGTT CTGAGCGGAA GGTCCTCATC AACACAGACC TGGGTTGGCC CAATGGCCTT ACCCTGGACT ATGATACCCG CAGGATCTAC TGGGTGGATG CGCATCTGGA CCGGATCGAG AGTGCTGACC TCAATGGGAA ACTGCGGCAG GTCTTGGTCA GCCATGTGTC CCACCCCTTT GCCCTCACAC AGCAAGACAG GTGGATCTAC TGGACAGACT GGCAGACCAA GTCAATCCAG CGTGTTGACA AATACTCAGG CCGGAACAAG GAGACAGTGC TGGCAAATGT GGAAGGACTC ATGGATATCA TCGTGGTTTC CCCTCAGCGG CAGACAGGGA CCAATGCCTG TGGTGTGAAC AATGGTGGCT GCACCCACCT CTGCTTTGCC AGAGCCTCGG ACTTCGTATG TGCCTGTCCT GACGAACCTG ATAGCCGGCC CTGCTCCCTT GTGCCTGGCC TGGTACCACC AGCTCCTAGG GCTACTGGCA TGAGTGAAAA GAGCCCAGTG CTACCCAACA CACCACCTAC CACCTTGTAT TCTTCAACCA CCCGGACCCG CACGTCTCTG GAGGAGGTGG AAGGAAGATG CTCTGAAAGG GATGCCAGGC TGGGCCTCTG TGCACGTTCC AATGACGCTG TTCCTGCTGC TCCAGGGGAA GGACTTCATA TCAGCTACGC CATTGGTGGA CTCCTCAGTA TTCTGCTGAT TTTGGTGGTG ATTGCAGCTT TGATGCTGTA CAGACACAAA AAATCCAAGT TCACTGATCC TGGAATGGGG AACCTCACCT ACAGCAACCC CTCCTACCGA ACATCCACAC AGGAAGTGAA GATTGAAGCA ATCCCCAAAC CAGCCATGTA CAACCAGCTG TGCTATAAGA AAGAGGGAGG GCCTGACCAT AACTACACCA AGGAGAAGAT CAAGATCGTA GAGGGAATCT GCCTCCTGTC TGGGGATGAT GCTGAGTGGG ATGACCTCAA GCAACTGCGA AGCTCACGGG GGGGCCTCCT CCGGGATCAT GTATGCATGA AGACAGACAC GGTGTCCATC CAGGCCAGCT CTGGCTCCCT GGATGACACA GAGACGGAGC AGCTGTTACA GGAAGAGCAG TCTGAGTGTA GCAGCGTCCA TACTGCAGCC ACTCCAGAAA GACGAGGCTC TCTGCCAGAC ACGGGCTGGA AACATGAACG CAAGCTCTCC TCAGAGAGCC AGGTCCTCGA GGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGTAG TGAGGGCCCG TTTAAACCCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTC