pLV3-CMV-COL22A1(human)(repeat-opt)-3×FLAG-CopGFP-Puro
$113.00
In stock
SKU
PL44401
100μg liquid plasmid(Instantly):$134
| name | pLV3-CMV-COL22A1(human)(repeat-opt)-3×FLAG-CopGFP-Puro | another name | apLV3-CMV-COL22A1(human)(repeat-opt)-3×FLAG-CopGFP-Puro |
| Insert Size (bp) | 4878bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGG CGGGTCTTAG AGGTAACGCT GTAGCTGGTC TTCTCTGGAT GTTGCTGCTT TGGTCAGGGG GAGGAGGATG TCAAGCCCAA CGAGCCGGAT GTAAGTCCGT GCATTACGAC CTGGTGTTCC TGCTGGACAC CAGCAGCAGC GTTGGCAAAG AGGATTTTGA AAAGGTGAGA CAGTGGGTGG CCAACCTGGT GGATACCTTC GAAGTCGGAC CAGATCGCAC TAGGGTAGGG GTCGTCCGGT ACTCCGACAG ACCTACCACT GCATTTGAGC TGGGGCTGTT TGGCTCTCAG GAGGAAGTGA AAGCTGCCGC AAGAAGGCTC GCGTACCATG GAGGCAACAC AAATACTGGG GACGCTCTCA GATACATCAC TGCACGATCA TTTAGTCCCC ATGCCGGAGG TCGGCCAAGG GATAGAGCTT ACAAGCAGGT GGCAATTCTG CTGACAGATG GCAGAAGCCA GGATCTCGTG CTGGACGCTG CTGCGGCTGC TCATAGAGCT GGGATCAGAA TCTTTGCCGT CGGTGTGGGA GAAGCATTGA AAGAAGAGCT CGAGGAGATA GCTAGTGAAC CCAAGTCAGC ACACGTGTTT CACGTGTCCG ATTTCAACGC CATAGACAAA ATAAGAGGCA AACTGCGGAG ACGGCTGTGC GAAAACGTAT TGTGTCCTAG CGTAAGGGTT GAGGGCGACC GCTTTAAGCA TACCAACGGG GGAACGAAAG AAATCACCGG GTTTGATCTG ATGGATCTGT TCTCCGTCAA GGAGATACTC GGGAAAAGAG AAAACGGGGC CCAGAGTTCA TACGTAAGAA TGGGCTCTTT CCCCGTTGTG CAGTCTACCG AAGATGTGTT CCCTCAGGGG CTTCCAGATG AGTACGCTTT TGTGACCACG TTTCGGTTCA GAAAAACTAG TCGGAAGGAG GACTGGTACA TCTGGCAGGT AATCGATCAG TATTCAATAC CACAAGTCTC CATCCGCCTT GATGGAGAAA ACAAAGCCGT GGAATACAAT GCCGTCGGCG CCATGAAAGA CGCGGTTAGG GTGGTCTTCC GCGGTTCCAG AGTTAACGAC CTGTTTGATC GAGACTGGCA TAAGATGGCA CTCAGCATCC AGGCCCAGAA TGTGTCCCTT CACATTGATT GCGCTCTGGT GCAGACGCTC CCCATCGAGG AGAGAGAGAA CATCGACATC CAGGGCAAAA CAGTCATCGG CAAAAGGCTG TACGATAGCG TGCCTATCGA TTTCGACTTG CAGCGAATTG TCATTTACTG CGACAGCCGG CATGCTGAAC TCGAGACCTG CTGCGACATT CCTTCCGGAC CATGCCAGGT GACCGTAGTG ACCGAACCAC CTCCTCCACC ACCTCCACAG AGACCACCTA CGCCAGGCTC TGAGCAGATC GGCTTTCTGA AGACCATCAA TTGTTCATGC CCAGCCGGAG AAAAAGGGGA GATGGGAGTG GCTGGTCCCA TGGGACTTCC CGGTCCTAAA GGTGATATAG GCGCAATAGG TCCTGTAGGG GCTCCAGGAC CAAAAGGGGA GAAAGGCGAT GTCGGAATCG GCCCTTTTGG CCAGGGGGAG AAGGGAGAGA AGGGTAGTCT TGGATTGCCG GGACCCCCAG GAAGAGATGG TAGCAAGGGA ATGCGAGGAG AACCTGGAGA ACTTGGGGAA CCAGGACTTC CTGGAGAAGT CGGTATGAGA GGACCTCAAG GTCCACCAGG ATTGCCAGGA CCTCCAGGTA GAGTCGGTGC ACCAGGACTT CAAGGAGAGC GCGGCGAAAA AGGCACTCGA GGAGAGAAAG GCGAAAGAGG TTTGGACGGA TTTCCTGGGA AACCTGGCGA TACAGGACAG CAAGGTAGGC CAGGACCTAG TGGCGTAGCA GGACCCCAAG GGGAGAAGGG TGATGTTGGA CCTGCTGGAC CACCTGGAGT TCCAGGGAGT GTTGTGCAAC AGGAAGGGCT GAAAGGGGAG CAAGGAGCTC CAGGTCCAAG AGGCCATCAA GGAGCTCCAG GACCACCTGG AGCTAGAGGA CCGATTGGTC CTGAAGGTAG AGATGGCCCG CCCGGATTGC AGGGTCTTAG AGGTAAGAAG GGTGATATGG GGCCACCAGG AATACCAGGA CTTCTTGGGT TGCAGGGACC TCCTGGACCT CCTGGAGTAC CTGGACCTCC AGGACCTGGA GGGAGTCCTG GACTTCCTGG AGAGATCGGC TTTCCCGGGA AACCAGGACC TCCAGGCCCT ACAGGTCCTC CAGGTAAGGA TGGACCAAAC GGACCTCCAG GCCCACCTGG TACTAAAGGA GAACCTGGAG AGCGCGGTGA AGATGGCCTC CCTGGTAAAC CTGGACTCAG GGGCGAGATA GGCGAGCAAG GCTTGGCTGG CAGACCAGGT GAAAAAGGAG AAGCCGGACT CCCTGGTGCT CCAGGTTTCC CTGGCGTGCG AGGAGAGAAG GGCGATCAGG GAGAGAAAGG CGAACTTGGA TTGCCAGGGC TGAAGGGGGA TAGGGGCGAA AAAGGAGAAG CAGGTCCTGC AGGTCCTCCC GGACTTCCTG GAACGACCAG CCTCTTCACT CCTCATCCTC GCATGCCAGG TGAACAGGGT CCAAAGGGCG AGAAGGGAGA TCCTGGCCTG CCTGGAGAGC CTGGTCTTCA GGGAAGGCCC GGAGAGCTGG GTCCACAAGG ACCAACAGGA CCTCCTGGAG CCAAAGGGCA AGAAGGAGCT CATGGTGCTC CAGGAGCAGC AGGAAATCCA GGAGCGCCTG GACACGTAGG AGCTCCAGGT CCATCCGGAC CACCAGGTTC AGTGGGAGCT CCTGGACTTA GAGGCACTCC AGGTAAAGAC GGCGAGCGAG GTGAAAAGGG TGCTGCTGGT GAGGAAGGCA GTCCAGGACC AGTAGGTCCA AGAGGAGACC CTGGGGCACC TGGATTGCCA GGTCCACCAG GTAAAGGGAA GGATGGCGAG CCTGGCCTTA GAGGAAGTCC TGGACTGCCA GGGCCTCTGG GAACAAAAGC TGCCTGCGGC AAGGTGAGGG GCTCAGAAAA CTGTGCCCTT GGCGGTCAGT GTGTGAAGGG TGATAGAGGA GCTCCAGGGA TCCCAGGATC ACCAGGTAGT AGAGGGGATC CGGGAATAGG CGTTGCAGGA CCACCGGGAC CTTCTGGTCC TCCTGGTGAT AAGGGATCCC CCGGAAGTAG GGGTCTTCCA GGATTTCCTG GGCCTCAAGG TCCAGCTGGT AGAGATGGAG CTCCTGGAAA TCCAGGAGAA AGAGGCCCCC CAGGGAAACC TGGCCTGTCA TCACTCCTGA GCCCCGGAGA TATCAATCTC CTTGCAAAAG ATGTCTGTAA TGATTGTCCA CCTGGTCCGC CTGGATTGCC TGGACTGCCT GGATTTAAGG GAGACAAGGG AGTACCCGGT AAGCCAGGTC GAGAGGGTAC AGAAGGAAAA AAGGGGGAAG CAGGTCCACC AGGACTGCCA GGTCCTCCTG GAATTGCAGG ACCACAAGGG TCACAGGGAG AAAGGGGAGC TGACGGAGAG GTCGGACAGA AAGGCGACCA GGGACATCCA GGAGTCCCTG GATTTATGGG GCCTCCTGGA AATCCAGGAC CACCTGGCGC TGATGGAATA GCAGGCGCAG CAGGACCTCC TGGAATCCAG GGATCTCCTG GGAAAGAAGG ACCTCCTGGA CCACAAGGAC CTTCAGGCCT GCCTGGAATT CCCGGGGAAG AGGGTAAGGA GGGAAGGGAT GGCAAACCCG GACCACCCGG AGAACCAGGT AAAGCTGGTG AACCTGGTCT TCCAGGACCA GAGGGTGCTA GAGGTCCTCC TGGGTTCAAA GGACATACTG GAGATTCTGG AGCGCCAGGT CCTAGAGGAG AATCCGGCGC TATGGGACTT CCAGGTCAGG AGGGTCTGCC TGGAAAAGAT GGAGATACAG GCCCTACAGG TCCACAGGGA CCCCAAGGAC CTAGAGGACC ACCGGGTAAG AATGGTAGTC CCGGATCACC TGGTGAACCT GGACCTTCCG GAACTCCAGG CCAGAAAGGC TCCAAAGGAG AAAATGGAAG CCCTGGTCTG CCTGGCTTTC TCGGACCAAG GGGACCACCA GGTGAACCAG GGGAAAAGGG CGTCCCAGGG AAAGAAGGAG TTCCTGGGAA ACCAGGGGAA CCTGGCTTTA AGGGGGAGAG AGGAGATCCT GGAATAAAAG GAGATAAAGG GCCTCCAGGA GGGAAAGGAC AACCTGGGGA TCCTGGAATC CCAGGGCATA AGGGCCACAC CGGTCTTATG GGACCTCAGG GCCTTCCTGG CGAAAACGGA CCAGTTGGTC CACCTGGTCC ACCAGGACAA CCAGGTTTTC CAGGACTGCG CGGAGAATCC CCTTCCATGG AGACCCTGCG GAGACTGATT CAGGAGGAAC TGGGGAAACA GCTGGAAACC CGGCTTGCCT ACCTGCTGGC ACAAATGCCT CCAGCCTACA TGAAAAGCTC ACAAGGTAGA CCTGGTCCAC CAGGACCACC AGGAAAGGAT GGTCTCCCTG GTAGAGCAGG ACCTATGGGT GAGCCTGGTA GACCTGGCCA GGGAGGTCTG GAAGGACCTA GTGGTCCCAT CGGACCAAAA GGGGAGAGAG GTGCCAAAGG CGATCCAGGT GCACCAGGAG TTGGGCTGAG AGGAGAAATG GGACCTCCAG GCATTCCTGG ACAGCCAGGC GAACCCGGAT ATGCAAAGGA TGGTCTCCCC GGAATACCCG GACCTCAGGG TGAAACAGGT CCCGCTGGAC ATCCAGGACT TCCCGGTCCT CCAGGACCTC CAGGTCAGTG TGACCCCTCC CAGTGTGCCT ACTTTGCTTC TCTCGCCGCA CGGCCCGGAA ACGTTAAGGG ACCTGGATCC GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTAGC GGCCGCGAAG GATCTGCGAT CGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC GGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GCTGAAGCTT CGAGGGGCTC GCATCTCTCC TTCACGCGCC CGCCGCCCTA CCTGAGGCCG CCATCCACGC CGGTTGAGTC GCGTTCTGCC GCCTCCCGCC TGTGGTGCCT CCTGAACTGC GTCCGCCGTC TAGGTAAGTT TAAAGCTCAG GTCGAGACCG GGCCTTTGTC CGGCGCTCCC TTGGAGCCTA CCTAGACTCA GCCGGCTCTC CACGCTTTGC CTGACCCTGC TTGCTCAACT CTACGTCTTT GTTTCGTTTT CTGTTCTGCG CCGTTACAGA TCCAAGCTGT GACCGGCGCC TACGCTAGAC GCCACCATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCAAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGGTGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGTGCTGGTG GGCAGCTTCG CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTT CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGCTGCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC CATCGCCTTC GCCAGATCCC GCGCTCAGTC GTCCAATTCT GCCGTGGACG GCACCGCCGG ACCCGGCTCC ACCGGATCTC GCGAGGGCAG AGGAAGTCTT CTAACATGCG GTGACGTGGA GGAGAATCCC GGCCCTATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGAAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTCCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGCCGCCTCC CCGCCTGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAAATAAG ATCTGCTTTT TGCTTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTAGTAGTT CATGTCATCT TATTATTCAG TATTTATAAC TTGCAAAGAA ATGAATATCA GAGAGTGAGA GGAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGCTCTAG CTATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GACTTTTGCA GAGACGGCCC AAATTCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT G
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