pCMV-3×FLAG-TET3(human)-Neo
NM_001287491.2;BEFAHRS; hCG_40738
100μg liquid plasmid(Instantly):$149
NM_001287491.2;BEFAHRS; hCG_40738
100μg liquid plasmid(Instantly):$149
| name | pCMV-3×FLAG-TET3(human)-Neo | another name | apCMV-3×FLAG-TET3(human)-Neo |
| Insert Size (bp) | 5388bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F:CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGCCACCA TGGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAAGACGATG ACGACAAGAT GAGCCAGTTT CAGGTGCCCC TGGCCGTCCA GCCGGACCTG CCAGGCCTTT ATGACTTCCC TCAGCGCCAG GTGATGGTAG GGAGCTTCCC GGGGTCTGGG CTCTCCATGG CTGGGAGTGA GTCCCAACTC CGAGGGGGTG GAGATGGTCG AAAGAAACGG AAACGGTGTG GTACTTGTGA GCCCTGCCGG CGGCTGGAAA ACTGTGGCGC TTGCACTAGC TGTACCAACC GCCGCACGCA CCAGATCTGC AAACTGCGAA AATGTGAGGT GCTGAAGAAA AAAGTAGGGC TTCTCAAGGA GGTGGAAATA AAGGCTGGTG AAGGAGCCGG GCCGTGGGGA CAAGGAGCGG CTGTCAAGAC AGGCTCAGAG CTCAGCCCAG TTGATGGACC TGTTCCAGGT CAGATGGACT CAGGGCCAGT GTACCATGGG GACTCACGGC AGCTAAGCGC CTCAGGGGTG CCGGTCAATG GTGCTAGAGA GCCCGCTGGA CCCAGTCTGC TGGGGACTGG GGGTCCTTGG CGGGTAGACC AAAAGCCCGA CTGGGAGGCT GCCCCAGGCC CAGCTCATAC TGCTCGCCTG GAAGATGCCC ACGATCTGGT GGCCTTTTCG GCTGTGGCCG AAGCTGTGTC CTCTTATGGG GCCCTTAGCA CCCGGCTCTA TGAAACCTTC AACCGTGAGA TGAGTCGTGA GGCTGGGAAC AACAGCAGGG GACCCCGGCC AGGGCCTGAG GGCTGCTCTG CTGGCAGCGA AGACCTTGAC ACACTGCAGA CGGCCCTGGC CCTCGCGCGG CATGGTATGA AACCACCCAA CTGCAACTGC GATGGCCCAG AATGCCCTGA CTACCTCGAG TGGCTGGAGG GGAAGATCAA GTCTGTGGTC ATGGAAGGAG GGGAGGAGCG GCCCAGGCTC CCAGGGCCTC TGCCTCCTGG TGAGGCCGGC CTCCCAGCAC CAAGCACCAG GCCACTCCTC AGCTCAGAGG TGCCCCAGAT CTCTCCCCAA GAGGGCCTGC CCCTGTCCCA GAGTGCCCTG AGCATTGCCA AGGAAAAAAA CATCAGCTTG CAGACCGCCA TTGCCATTGA GGCCCTCACA CAGCTCTCCT CTGCCCTCCC GCAGCCTTCT CATTCCACCC CCCAGGCTTC TTGCCCCCTT CCTGAGGCCT TGTCACCTCC TGCCCCTTTC AGATCTCCCC AGTCTTACCT CCGGGCTCCC TCATGGCCTG TGGTTCCTCC TGAAGAGCAC TCATCTTTTG CTCCTGATAG CTCTGCCTTC CCTCCAGCAA CTCCTAGAAC TGAGTTCCCT GAAGCCTGGG GCACTGACAC CCCTCCAGCA ACGCCCCGGA GCTCCTGGCC CATGCCTCGC CCAAGCCCCG ATCCCATGGC TGAACTGGAG CAGTTGTTGG GCAGCGCCAG TGATTACATC CAGTCAGTAT TCAAGCGGCC TGAGGCCCTG CCTACCAAGC CCAAGGTCAA GGTGGAGGCA CCCTCTTCCT CCCCGGCCCC GGCCCCATCC CCTGTACTTC AGAGGGAGGC TCCCACGCCA TCCTCGGAGC CCGACACCCA CCAGAAGGCC CAGACCGCCC TGCAGCAGCA CCTCCACCAC AAGCGCAGCC TCTTCCTAGA ACAGGTGCAC GACACCTCCT TCCCTGCTCC TTCAGAGCCT TCTGCTCCTG GCTGGTGGCC CCCACCAAGT TCACCTGTCC CACGGCTTCC AGACAGACCA CCCAAGGAGA AGAAGAAGAA GCTCCCAACA CCAGCTGGAG GTCCCGTGGG AACGGAGAAA GCTGCCCCTG GGATCAAGCC CAGTGTCCGA AAGCCCATTC AGATCAAGAA GTCCAGGCCC CGGGAAGCAC AGCCCCTCTT CCCACCTGTC CGACAGATTG TCCTGGAAGG GCTTAGGTCC CCAGCCTCCC AGGAAGTGCA GGCTCATCCA CCGGCCCCTC TGCCTGCCTC ACAGGGCTCT GCTGTGCCCC TGCCCCCAGA ACCTTCTCTT GCGCTATTTG CACCTAGTCC CTCCAGGGAC AGCCTGCTGC CCCCTACTCA GGAAATGAGG TCCCCCAGCC CCATGACAGC CTTGCAGCCA GGCTCCACTG GCCCTCTTCC CCCTGCCGAT GACAAGCTGG AAGAGCTCAT CCGGCAGTTT GAGGCTGAAT TTGGAGATAG CTTTGGGCTT CCCGGCCCCC CTTCTGTGCC CATTCAGGAC CCCGAGAACC AGCAAACATG TCTCCCAGCC CCTGAGAGCC CCTTTGCTAC CCGTTCCCCC AAGCAAATCA AGATTGAGTC TTCGGGGGCT GTGACTGTGC TCTCAACCAC CTGCTTCCAT TCAGAGGAGG GAGGACAGGA GGCCACACCC ACCAAGGCTG AGAACCCACT CACACCCACC CTCAGTGGCT TCTTGGAGTC ACCTCTTAAG TACCTGGACA CACCCACCAA GAGTCTGCTG GACACACCTG CCAAGAGAGC CCAGGCCGAG TTCCCCACCT GCGATTGCGT CGAACAAATA GTGGAGAAAG ATGAAGGTCC ATATTATACT CACTTGGGAT CTGGCCCCAC GGTCGCCTCT ATCCGGGAAC TCATGGAGGA GCGGTATGGA GAGAAGGGGA AAGCCATCCG GATCGAGAAG GTCATCTACA CGGGGAAGGA GGGAAAGAGC TCCCGCGGTT GCCCCATTGC AAAGTGGGTG ATCCGCAGGC ACACGCTGGA GGAGAAGCTA CTCTGCCTGG TGCGGCACCG GGCAGGCCAC CACTGCCAGA ACGCTGTGAT CGTCATCCTC ATCCTGGCCT GGGAGGGCAT TCCCCGTAGC CTCGGAGACA CCCTCTACCA GGAGCTCACC GACACCCTCC GGAAGTATGG GAACCCCACC AGCCGGAGAT GCGGCCTCAA CGATGACCGG ACCTGCGCTT GCCAAGGCAA AGACCCCAAC ACCTGTGGTG CCTCCTTCTC CTTTGGTTGT TCCTGGAGCA TGTACTTCAA CGGCTGCAAG TATGCTCGGA GCAAGACACC TCGCAAGTTC CGCCTCGCAG GGGACAATCC CAAAGAGGAA GAAGTGCTCC GGAAGAGTTT CCAGGACCTG GCCACCGAAG TCGCTCCCCT GTACAAGCGA CTGGCCCCTC AGGCCTATCA GAACCAGGTG ACCAACGAGG AAATAGCGAT TGACTGCCGT CTGGGGCTGA AGGAAGGACG GCCCTTCGCG GGGGTCACGG CCTGCATGGA CTTCTGTGCC CACGCCCACA AGGACCAGCA TAACCTCTAC AATGGGTGCA CCGTGGTCTG CACCCTGACC AAGGAAGACA ATCGCTGCGT GGGCAAGATT CCCGAGGATG AGCAGCTGCA TGTTCTCCCC CTGTACAAGA TGGCCAACAC GGATGAGTTT GGTAGCGAGG AGAACCAGAA TGCAAAGGTG GGCAGCGGAG CCATCCAGGT GCTCACCGCC TTCCCCCGCG AGGTCCGACG CCTGCCCGAG CCTGCCAAGT CCTGCCGCCA GCGGCAGCTG GAAGCCAGAA AGGCAGCAGC CGAGAAGAAG AAGATTCAGA AGGAGAAGCT GAGCACTCCG GAGAAGATCA AGCAGGAGGC CCTGGAGCTG GCGGGCATTA CGTCGGACCC AGGCCTGTCT CTGAAGGGTG GATTGTCCCA GCAAGGCCTG AAGCCCTCCC TCAAGGTGGA GCCGCAGAAC CACTTCAGCT CCTTCAAGTA CAGCGGCAAC GCGGTGGTGG AGAGCTACTC GGTGCTGGGC AACTGCCGGC CCTCCGACCC TTACAGCATG AACAGCGTGT ACTCCTACCA CTCCTACTAT GCACAGCCCA GCCTGACCTC CGTCAATGGC TTCCACTCCA AGTACGCTCT CCCGTCTTTT AGCTACTATG GCTTTCCATC CAGCAACCCC GTCTTCCCCT CTCAGTTCCT GGGTCCTGGT GCCTGGGGGC ACAGTGGCAG CAGTGGCAGT TTTGAGAAGA AGCCAGACCT CCACGCTCTG CACAACAGCC TGAGCCCGGC CTACGGTGGT GCTGAGTTTG CCGAGCTGCC CAGCCAGGCT GTTCCCACAG ACGCCCACCA CCCCACTCCT CACCACCAGC AGCCTGCGTA CCCAGGCCCC AAGGAGTATC TGCTTCCCAA GGCCCCCCTA CTCCACTCAG TGTCCAGGGA CCCCTCCCCC TTTGCCCAGA GCTCCAACTG CTACAACAGA TCCATCAAGC AAGAGCCAGT AGACCCGCTG ACCCAGGCTG AGCCTGTGCC CAGAGACGCT GGCAAGATGG GCAAGACACC TCTGTCCGAG GTGTCTCAGA ATGGAGGACC CAGTCACCTT TGGGGACAGT ACTCAGGAGG CCCAAGCATG TCCCCCAAGA GGACTAACGG TGTGGGTGGC AGCTGGGGTG TGTTCTCGTC TGGGGAGAGT CCTGCCATCG TCCCTGACAA GCTCAGTTCC TTTGGGGCCA GCTGCCTGGC CCCTTCCCAC TTCACAGATG GCCAGTGGGG GCTGTTCCCC GGTGAGGGGC AGCAGGCAGC TTCCCACTCT GGAGGACGGC TGCGAGGCAA ACCGTGGAGC CCCTGCAAGT TTGGGAACAG CACCTCGGCC TTGGCTGGGC CCAGCCTGAC TGAGAAGCCG TGGGCGCTGG GGGCAGGGGA TTTCAACTCG GCCCTGAAAG GTAGTCCTGG GTTCCAAGAC AAGCTGTGGA ACCCCATGAA AGGAGAGGAG GGCAGGATTC CAGCCGCAGG GGCCAGCCAG CTGGACAGGG CCTGGCAGTC CTTTGGTCTG CCCCTGGGAT CCAGCGAGAA GCTGTTTGGG GCTCTGAAGT CAGAGGAGAA GCTGTGGGAC CCCTTCAGCC TGGAGGAGGG GCCGGCTGAG GAGCCCCCCA GCAAGGGAGC GGTGAAGGAG GAGAAGGGCG GTGGTGGTGC GGAGGAGGAA GAGGAGGAGC TGTGGTCGGA CAGTGAACAC AACTTCCTGG ACGAGAACAT CGGCGGCGTG GCCGTGGCCC CAGCCCACGG CTCCATCCTC ATCGAGTGTG CCCGGCGGGA GCTGCACGCC ACCACGCCGC TTAAGAAGCC CAACCGCTGC CACCCCACCC GCATCTCGCT GGTCTTCTAC CAGCACAAGA ACCTCAACCA GCCCAACCAC GGGCTGGCCC TCTGGGAAGC CAAGATGAAG CAGCTGGCGG AGAGGGCACG GGCACGGCAG GAGGAGGCTG CCCGGCTGGG CCTGGGCCAG CAGGAGGCCA AGCTCTACGG GAAGAAGCGC AAGTGGGGGG GCACTGTGGT TGCTGAGCCC CAGCAGAAAG AGAAGAAGGG GGTCGTCCCC ACCCGGCAGG CACTGGCTGT GCCCACAGAC TCGGCGGTCA CCGTGTCCTC CTATGCCTAC ACGAAGGTCA CTGGCCCCTA CAGCCGCTGG ATCTAGGAAT TCTGCAGATA TCCAGCACAG TGGCGGCCGC TCGAGTCTAG AGGGCCCGTT TAAACCCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA AAGAACCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGT