NM_007742.4;Col1a-1; Cola-1; Cola1; Mov-13; Mov13
100μg liquid plasmid(Instantly):$134
| name | pDC316-mCMV-Col1a1(mouse)-3×FLAG | another name | apDC316-mCMV-Col1a1(mouse)-3×FLAG |
| Insert Size (bp) | 4362bp | Plasmid host | Mammalian cell,Adenovirus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | mCMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTCATCAATA ATATACCTTA TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGCTCTAGA GATATACTGA GTCATTAGGG ACTTTCCAAT GGGTTTTGCC CAGTACATAA GGTCAATAGG GGTGAATCAA CAGGAAAGTC CCATTGGAGC CAAGTACACT GAGTCAATAG GGACTTTCCA TTGGGTTTTG CCCAGTACAA AAGGTCAATA GGGGGTGAGT CAATGGGTTT TTCCCATTAT TGGCACGTAC ATAAGGTCAA TAGGGGTGAG TCATTGGGTT TTTCCAGCCA ATTTAATTAA AACGCCATGT ACTTTCCCAC CATTGACGTC AATGGGCTAT TGAAACTAAT GCAACGTGAC CTTTAAACGG TACTTTCCCA TAGCTGATTA ATGGGAAAGT ACCGTTCTCG AGCCAATACA CGTCAATGGG AAGTGAAAGG GCAGCCAAAA CGTAACACCG CCCCGGTTTT CCCCTGGAAA TTCCATATTG GCACGCATTC TATTGGCTGA GCTGCGTTCT ACGTGGGTAT AAGAGGCGCG ACCAGCGTCG GTACCGTCGC AGTCTTCGGT CTGACCACCG TAGAACGCAG ATCGAATTCG CCACCATGTT CAGCTTTGTG GACCTCCGGC TCCTGCTCCT CTTAGGGGCC ACTGCCCTCC TGACGCATGG CCAAGAAGAC ATCCCTGAAG TCAGCTGCAT ACACAATGGC CTAAGGGTCC CCAATGGTGA GACGTGGAAA CCCGAGGTAT GCTTGATCTG TATCTGCCAC AATGGCACGG CTGTGTGCGA TGACGTGCAA TGCAATGAAG AACTGGACTG TCCCAACCCC CAAAGACGGG AGGGCGAGTG CTGTGCTTTC TGCCCGGAAG AATACGTATC ACCAAACTCA GAAGATGTAG GAGTCGAGGG ACCCAAGGGA GACCCTGGCC CCCAAGGCCC AAGGGGACCC GTTGGCCCCC CTGGACGAGA TGGCATCCCT GGACAGCCTG GACTTCCTGG TCCTCCTGGT CCCCCTGGGC CCCCCGGACC CCCTGGCCTT GGAGGAAACT TTGCTTCCCA GATGTCCTAT GGCTATGATG AAAAATCAGC TGGAGTTTCC GTGCCTGGCC CCATGGGTCC TTCTGGTCCT CGTGGTCTCC CTGGCCCCCC TGGTGCACCT GGTCCACAAG GTTTCCAAGG CCCCCCTGGT GAACCTGGCG AGCCTGGCGG TTCAGGTCCA ATGGGTCCCC GAGGTCCCCC TGGCCCTCCT GGCAAGAATG GAGATGATGG GGAAGCTGGC AAGCCCGGCC GTCCTGGTGA GCGTGGACCT CCTGGACCTC AGGGTGCTCG TGGATTGCCT GGAACAGCTG GCCTCCCTGG AATGAAGGGA CACCGAGGCT TCAGTGGTTT GGATGGTGCC AAAGGAGATG CTGGTCCTGC TGGTCCTAAG GGAGAGCCCG GCAGTCCTGG TGAAAACGGA GCTCCTGGCC AGATGGGTCC CCGAGGTCTG CCCGGTGAGA GAGGTCGCCC TGGACCTCCT GGCACTGCTG GTGCTCGCGG TAACGATGGT GCTGTTGGTG CTGCTGGACC CCCTGGTCCC ACCGGCCCCA CTGGCCCTCC TGGCTTCCCT GGTGCAGTTG GTGCTAAGGG TGAAGCTGGT CCCCAAGGAG CTAGAGGCTC TGAAGGTCCC CAGGGTGTGC GTGGTGAGCC CGGACCCCCT GGCCCTGCTG GTGCTGCCGG CCCTGCTGGA AACCCTGGTG CTGATGGACA ACCTGGCGCT AAAGGTGCCA ATGGTGCTCC TGGTATTGCT GGTGCTCCTG GCTTCCCTGG TGCCCGAGGC CCCTCTGGAC CCCAGGGCCC CAGCGGCCCT CCAGGTCCCA AGGGTAACAG TGGTGAACCT GGTGCTCCTG GCAACAAAGG AGACACTGGT GCCAAAGGAG AACCCGGTGC TACTGGAGTT CAAGGTCCCC CAGGCCCTGC CGGAGAAGAA GGAAAACGAG GAGCCCGTGG TGAGCCTGGA CCTTCCGGAC TGCCTGGACC TCCTGGCGAG CGTGGTGGAC CTGGTAGCCG TGGTTTCCCT GGTGCTGATG GTGTTGCTGG CCCCAAGGGT CCTTCCGGTG AACGTGGTGC TCCCGGACCT GCTGGTCCCA AAGGTTCTCC TGGTGAAGCT GGTCGCCCCG GTGAAGCTGG TCTCCCTGGT GCCAAGGGTC TCACTGGCAG TCCTGGCAGC CCTGGTCCTG ATGGCAAAAC CGGCCCCCCT GGTCCCGCTG GTCAAGATGG TCGCCCTGGA CCCGCAGGTC CTCCTGGAGC CCGTGGCCAG GCTGGTGTGA TGGGATTCCC TGGACCTAAG GGTACCGCTG GAGAACCTGG AAAGGCTGGA GAGCGAGGCC TTCCCGGACC CCCTGGCGCT GTTGGTCCTG CTGGCAAAGA TGGAGAAGCT GGAGCTCAGG GAGCCCCTGG CCCTGCTGGT CCTGCTGGTG AGAGAGGTGA ACAAGGTCCC GCTGGCTCCC CTGGATTCCA GGGTCTTCCT GGTCCTGCCG GTCCTCCTGG TGAAGCAGGC AAGCCTGGTG AACAGGGTGT TCCTGGAGAC CTTGGTGCCC CTGGACCCTC TGGCGCAAGA GGCGAGAGAG GTTTCCCTGG TGAACGTGGT GTACAAGGTC CCCCAGGTCC TGCTGGTCCC CGAGGAAACA ATGGTGCCCC CGGCAACGAT GGTGCCAAGG GTGATACTGG TGCCCCCGGA GCTCCCGGTA GCCAGGGTGC CCCCGGTCTT CAGGGAATGC CTGGTGAACG TGGTGCAGCT GGTCTTCCAG GTCCTAAGGG TGACAGAGGT GATGCTGGTC CCAAAGGTGC TGATGGTTCT CCTGGTAAAG ATGGTGCCCG TGGTCTGACT GGTCCCATTG GTCCTCCTGG CCCTGCTGGT GCCCCTGGTG ACAAGGGTGA AGCTGGTCCC AGTGGTCCTC CCGGTCCCAC CGGAGCCCGT GGTGCTCCCG GAGACCGTGG TGAGGCTGGT CCCCCTGGTC CTGCTGGCTT TGCCGGCCCC CCTGGTGCTG ATGGCCAACC TGGTGCGAAA GGTGAACCTG GTGATACTGG TGTTAAAGGT GATGCTGGTC CTCCTGGCCC TGCTGGTCCT GCTGGACCCC CCGGCCCCAT TGGTAACGTT GGTGCTCCTG GACCCAAAGG TCCTCGTGGT GCTGCTGGTC CCCCTGGTGC TACTGGCTTC CCTGGTGCTG CTGGCCGTGT CGGTCCCCCT GGTCCCTCTG GAAATGCTGG ACCCCCTGGC CCTCCCGGTC CCGTTGGCAA AGAAGGGGGC AAAGGTCCCC GTGGTGAGAC TGGCCCTGCT GGACGTCCTG GTGAAGTTGG TCCCCCAGGT CCCCCCGGTC CTGCTGGTGA GAAAGGATCT CCTGGTGCTG ATGGACCTGC TGGCTCTCCT GGTACCCCTG GACCTCAGGG TATTGCTGGA CAACGTGGTG TGGTCGGTCT TCCCGGTCAG AGAGGAGAAA GAGGCTTCCC TGGTCTTCCT GGCCCCTCTG GTGAACCTGG CAAACAAGGT CCTTCTGGAT CAAGTGGTGA ACGCGGTCCC CCTGGCCCCA TGGGGCCCCC TGGATTGGCT GGTCCCCCTG GTGAATCTGG ACGTGAGGGA TCCCCTGGTG CTGAAGGCTC CCCTGGAAGG GATGGTGCTC CCGGGGCCAA GGGTGACCGT GGTGAGACTG GCCCCGCTGG CCCCCCTGGT GCCCCTGGTG CTCCCGGTGC TCCCGGCCCT GTTGGTCCCG CTGGCAAGAA TGGCGATCGT GGTGAGACTG GTCCTGCTGG TCCTGCTGGT CCCATTGGCC CTGCTGGTGC CCGTGGCCCT GCTGGACCCC AAGGCCCCCG TGGTGACAAG GGTGAGACAG GCGAACAAGG TGACAGAGGC ATAAAGGGTC ATCGTGGCTT CTCTGGTCTC CAGGGTCCTC CTGGTTCTCC TGGTTCTCCT GGTGAACAAG GCCCCTCTGG AGCTTCAGGT CCTGCAGGCC CCCGGGGTCC CCCTGGCTCT GCTGGTTCTC CTGGCAAAGA CGGACTCAAC GGTCTCCCTG GCCCCATTGG TCCCCCTGGT CCTCGAGGTC GCACTGGTGA CAGCGGCCCT GCTGGTCCCC CCGGCCCTCC TGGACCCCCT GGCCCTCCTG GACCTCCCAG TGGCGGTTAT GACTTCAGCT TCCTGCCTCA GCCACCTCAA GAGAAGTCTC AAGATGGTGG CCGCTACTAC CGGGCCGATG ATGCTAACGT GGTTCGTGAC CGTGACCTTG AGGTGGACAC CACCCTCAAG AGCCTGAGTC AGCAGATTGA GAACATCCGC AGCCCCGAAG GCAGCCGCAA GAACCCTGCC CGCACATGCC GCGACCTCAA GATGTGCCAC TCTGACTGGA AGAGCGGAGA GTACTGGATC GACCCTAACC AAGGCTGCAA CCTGGACGCC ATCAAGGTCT ACTGCAACAT GGAGACAGGT CAGACCTGTG TGTTCCCTAC TCAGCCGTCT GTGCCTCAGA AGAACTGGTA CATCAGCCCG AACCCCAAGG AAAAGAAGCA CGTCTGGTTT GGAGAGAGCA TGACCGATGG ATTCCCGTTC GAGTACGGAA GCGAGGGCTC CGACCCCGCC GATGTCGCTA TCCAGCTGAC CTTCCTGCGC CTAATGTCCA CCGAGGCCTC CCAGAACATC ACCTATCACT GCAAGAACAG CGTAGCCTAC ATGGACCAGC AGACTGGCAA CCTCAAGAAG GCCCTGCTCC TCCAGGGATC CAACGAGATC GAGCTCAGAG GCGAAGGCAA CAGTCGCTTC ACCTACAGCA CCCTTGTGGA CGGCTGCACG AGTCACACCG GAACTTGGGG CAAGACAGTC ATCGAATACA AAACCACCAA GACCTCCCGC CTGCCCATCA TCGATGTGGC TCCCTTGGAC ATTGGTGCCC CAGACCAGGA ATTCGGACTA GACATTGGCC CTGCCTGCTT CGTGAAGCTT GATTACAAGG ATGACGACGA TAAGGACTAT AAGGACGATG ATGACAAGGA CTACAAAGAT GATGACGATA AATAGTCGAC TTCGAGCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA TCGTCTAGCA TCGAAGATCC AATAACTTCG TATAGCATAC ATTATACGAA GTTATAAGTA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAACACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACTCTAGG CAAAATAGCA CCCTCCCGCT CCAGAACAAC ATACAGCGCT TCACAGCGGC AGCCTAACAG TCAGCCTTAC CAGTAAAAAA GAAAACCTAT TAAAAAAACA CCACTCGACA CGGCACCAGC TCAATCAGTC ACAGTGTAAA AAAGGGCCAA GTGCAGAGCG AGTATATATA GGACTAAAAA ATGACGTAAC GGTTAAAGTC CACAAAAAAC ACCCAGAAAA CCGCACGCGA ACCTACGCCC AGAAACGAAA GCCAAAAAAC CCACAACTTC CTCAAATCGT CACTTCCGTT TTCCCACGTT ACGTAACTTC CCATTTTAAG AAAACTACAA TTCCCAACAC ATACAAGTTA CTCCGCCCTA AAACCTACGT CACCCGCCCC GTTCCCACGC CCCGCGCCAC GTCACAAACT CCACCCCCTC ATTATCATAT TGGCTTCAAT CCAAAATAAG GTATA
