NM_001406365.1;bA119F7.1; CXXC6; LCX
100μg liquid plasmid(Instantly):$163
| name | pCMV-dPspCas13b-TET1(human)(1synonymous mutations) | another name | apCMV-dPspCas13b-TET1(human)(1synonymous mutations) |
| Insert Size (bp) | 6498bp | Plasmid host | Mammary cell |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGCCACCA TGAACATCCC CGCTCTGGTG GAAAACCAGA AGAAGTACTT TGGCACCTAC AGCGTGATGG CCATGCTGAA CGCTCAGACC GTGCTGGACC ACATCCAGAA GGTGGCCGAT ATTGAGGGCG AGCAGAACGA GAACAACGAG AATCTGTGGT TTCACCCCGT GATGAGCCAC CTGTACAACG CCAAGAACGG CTACGACAAG CAGCCCGAGA AAACCATGTT CATCATCGAG CGGCTGCAGA GCTACTTCCC ATTCCTGAAG ATCATGGCCG AGAACCAGAG AGAGTACAGC AACGGCAAGT ACAAGCAGAA CCGCGTGGAA GTGAACAGCA ACGACATCTT CGAGGTGCTG AAGCGCGCCT TCGGCGTGCT GAAGATGTAC AGGGACCTGA CCAACGCATA CAAGACCTAC GAGGAAAAGC TGAACGACGG CTGCGAGTTC CTGACCAGCA CAGAGCAACC TCTGAGCGGC ATGATCAACA ACTACTACAC AGTGGCCCTG CGGAACATGA ACGAGAGATA CGGCTACAAG ACAGAGGACC TGGCCTTCAT CCAGGACAAG CGGTTCAAGT TCGTGAAGGA CGCCTACGGC AAGAAAAAGT CCCAAGTGAA TACCGGATTC TTCCTGAGCC TGCAGGACTA CAACGGCGAC ACACAGAAGA AGCTGCACCT GAGCGGAGTG GGAATCGCCC TGCTGATCTG CCTGTTCCTG GACAAGCAGT ACATCAACAT CTTTCTGAGC AGGCTGCCCA TCTTCTCCAG CTACAATGCC CAGAGCGAGG AACGGCGGAT CATCATCAGA TCCTTCGGCA TCAACAGCAT CAAGCTGCCC AAGGACCGGA TCCACAGCGA GAAGTCCAAC AAGAGCGTGG CCATGGATAT GCTCAACGAA GTGAAGCGGT GCCCCGACGA GCTGTTCACA ACACTGTCTG CCGAGAAGCA GTCCCGGTTC AGAATCATCA GCGACGACCA CAATGAAGTG CTGATGAAGC GGAGCAGCGA CAGATTCGTG CCTCTGCTGC TGCAGTATAT CGATTACGGC AAGCTGTTCG ACCACATCAG GTTCCACGTG AACATGGGCA AGCTGAGATA CCTGCTGAAG GCCGACAAGA CCTGCATCGA CGGCCAGACC AGAGTCAGAG TGATCGAGCA GCCCCTGAAC GGCTTCGGCA GACTGGAAGA GGCCGAGACA ATGCGGAAGC AAGAGAACGG CACCTTCGGC AACAGCGGCA TCCGGATCAG AGACTTCGAG AACATGAAGC GGGACGACGC CAATCCTGCC AACTATCCCT ACATCGTGGA CACCTACACA CACTACATCC TGGAAAACAA CAAGGTCGAG ATGTTTATCA ACGACAAAGA GGACAGCGCC CCACTGCTGC CCGTGATCGA GGATGATAGA TACGTGGTCA AGACAATCCC CAGCTGCCGG ATGAGCACCC TGGAAATTCC AGCCATGGCC TTCCACATGT TTCTGTTCGG CAGCAAGAAA ACCGAGAAGC TGATCGTGGA CGTGCACAAC CGGTACAAGA GACTGTTCCA GGCCATGCAG AAAGAAGAAG TGACCGCCGA GAATATCGCC AGCTTCGGAA TCGCCGAGAG CGACCTGCCT CAGAAGATCC TGGATCTGAT CAGCGGCAAT GCCCACGGCA AGGATGTGGA CGCCTTCATC AGACTGACCG TGGACGACAT GCTGACCGAC ACCGAGCGGA GAATCAAGAG ATTCAAGGAC GACCGGAAGT CCATTCGGAG CGCCGACAAC AAGATGGGAA AGAGAGGCTT CAAGCAGATC TCCACAGGCA AGCTGGCCGA CTTCCTGGCC AAGGACATCG TGCTGTTTCA GCCCAGCGTG AACGATGGCG AGAACAAGAT CACCGGCCTG AACTACCGGA TCATGCAGAG CGCCATTGCC GTGTACGATA GCGGCGACGA TTACGAGGCC AAGCAGCAGT TCAAGCTGAT GTTCGAGAAG GCCCGGCTGA TCGGCAAGGG CACAACAGAG CCTCATCCAT TTCTGTACAA GGTGTTCGCC CGCAGCATCC CCGCCAATGC CGTCGAGTTC TACGAGCGCT ACCTGATCGA GCGGAAGTTC TACCTGACCG GCCTGTCCAA CGAGATCAAG AAAGGCAACA GAGTGGATGT GCCCTTCATC CGGCGGGACC AGAACAAGTG GAAAACACCC GCCATGAAGA CCCTGGGCAG AATCTACAGC GAGGATCTGC CCGTGGAACT GCCCAGACAG ATGTTCGACA ATGAGATCAA GTCCCACCTG AAGTCCCTGC CACAGATGGA AGGCATCGAC TTCAACAATG CCAACGTGAC CTATCTGATC GCCGAGTACA TGAAGAGAGT GCTGGACGAC GACTTCCAGA CCTTCTACCA GTGGAACCGC AACTACCGGT ACATGGACAT GCTTAAGGGC GAGTACGACA GAAAGGGCTC CCTGCAGCAC TGCTTCACCA GCGTGGAAGA GAGAGAAGGC CTCTGGAAAG AGCGGGCCTC CAGAACAGAG CGGTACAGAA AGCAGGCCAG CAACAAGATC CGCAGCAACC GGCAGATGAG AAACGCCAGC AGCGAAGAGA TCGAGACAAT CCTGGATAAG CGGCTGAGCA ACAGCCGGAA CGAGTACCAG AAAAGCGAGA AAGTGATCCG GCGCTACAGA GTGCAGGATG CCCTGCTGTT TCTGCTGGCC AAAAAGACCC TGACCGAACT GGCCGATTTC GACGGCGAGA GGTTCAAACT GAAAGAAATC ATGCCCGACG CCGAGAAGGG AATCCTGAGC GAGATCATGC CCATGAGCTT CACCTTCGAG AAAGGCGGCA AGAAGTACAC CATCACCAGC GAGGGCATGA AGCTGAAGAA CTACGGCGAC TTCTTTGTGC TGGCTAGCGA CAAGAGGATC GGCAACCTGC TGGAACTCGT GGGCAGCGAC ATCGTGTCCA AAGAGGATAT CATGGAAGAG TTCAACAAAT ACGACCAGTG CAGGCCCGAG ATCAGCTCCA TCGTGTTCAA CCTGGAAAAG TGGGCCTTCG ACACATACCC CGAGCTGTCT GCCAGAGTGG ACCGGGAAGA GAAGGTGGAC TTCAAGAGCA TCCTGAAAAT CCTGCTGAAC AACAAGAACA TCAACAAAGA GCAGAGCGAC ATCCTGCGGA AGATCCGGAA CGCCTTCGAT GCAAACAATT ACCCCGACAA AGGCGTGGTG GAAATCAAGG CCCTGCCTGA GATCGCCATG AGCATCAAGA AGGCCTTTGG GGAGTACGCC ATCATGAAGG GAAGCCTGCA GCTGCCTCCA CTTGAAAGAC TGACACTGGG ATCCGGAGGA GGTGGAAGCA TGTCTCGATC CCGCCATGCA AGGCCTTCCA GATTAGTCAG GAAGGAAGAT GTAAACAAAA AAAAGAAAAA CAGCCAACTA CGAAAGACAA CCAAGGGAGC CAACAAAAAT GTGGCATCAG TCAAGACTTT AAGCCCTGGA AAATTAAAGC AATTAATTCA AGAAAGAGAT GTTAAGAAAA AAACAGAACC TAAACCACCC GTGCCAGTCA GAAGCCTTCT GACAAGAGCT GGAGCAGCAC GCATGAATTT GGATAGGACT GAGGTTCTTT TTCAGAACCC AGAGTCCTTA ACCTGCAATG GGTTTACAAT GGCGCTACGA AGCACCTCTC TTAGCAGGCG ACTCTCCCAA CCCCCACTGG TCGTAGCCAA ATCCAAAAAG GTTCCACTTT CTAAGGGTTT AGAAAAGCAA CATGATTGTG ATTATAAGAT ACTCCCTGCT TTGGGAGTAA AGCACTCAGA AAATGATTCG GTTCCAATGC AAGACACCCA AGTCCTTCCT GATATAGAGA CTCTAATTGG TGTACAAAAT CCCTCTTTAC TTAAAGGTAA GAGCCAAGAG ACAACTCAGT TTTGGTCCCA AAGAGTTGAG GATTCCAAGA TCAATATCCC TACCCACAGT GGCCCTGCAG CTGAGATCCT TCCTGGGCCA CTGGAAGGGA CACGCTGTGG TGAAGGACTA TTCTCTGAAG AGACATTGAA TGATACCAGT GGTTCCCCAA AAATGTTTGC TCAGGACACA GTGTGTGCTC CTTTTCCCCA AAGAGCAACC CCCAAAGTTA CCTCTCAAGG AAACCCCAGC ATTCAGTTAG AAGAGTTGGG TTCACGAGTA GAATCTCTTA AGTTATCTGA TTCTTACCTG GATCCCATTA AAAGTGAACA TGATTGCTAC CCCACCTCCA GTCTTAATAA GGTTATACCT GACTTGAACC TTAGAAACTG CTTGGCTCTT GGTGGGTCTA CGTCTCCTAC CTCTGTAATA AAATTCCTCT TGGCAGGCTC AAAACAAGCG ACCCTTGGTG CTAAACCAGA TCATCAAGAG GCCTTCGAAG CTACTGCAAA TCAACAGGAA GTTTCTGATA CCACCTCTTT CCTAGGACAG GCCTTTGGTG CTATCCCACA TCAATGGGAA CTTCCTGGTG CTGACCCAGT TCATGGTGAG GCCCTGGGTG AGACCCCAGA TCTACCAGAG ATTCCTGGTG CTATTCCAGT CCAAGGAGAG GTCTTTGGTA CTATTTTAGA CCAACAAGAA ACTCTTGGTA TGAGTGGGAG TGTTGTCCCA GACTTGCCTG TCTTCCTTCC TGTTCCTCCA AATCCAATTG CTACCTTTAA TGCTCCTTCC AAATGGCCTG AGCCCCAAAG CACTGTCTCA TATGGACTTG CAGTCCAGGG TGCTATACAG ATTTTGCCTT TGGGCTCAGG ACACACTCCT CAATCATCAT CAAACTCAGA GAAAAATTCA TTACCTCCAG TAATGGCTAT AAGCAATGTA GAAAATGAGA AGCAGGTTCA TATAAGCTTC CTGCCAGCTA ACACTCAGGG GTTCCCATTA GCCCCTGAGA GAGGACTCTT CCATGCTTCA CTGGGTATAG CCCAACTCTC TCAGGCTGGT CCTAGCAAAT CAGACAGAGG GAGCTCCCAG GTCAGTGTAA CCAGCACAGT TCATGTTGTC AACACCACAG TGGTGACTAT GCCAGTGCCA ATGGTCAGTA CCTCCTCTTC TTCCTATACC ACTTTGCTAC CGACTTTGGA AAAGAAGAAA AGAAAGCGAT GTGGGGTCTG TGAACCCTGC CAGCAGAAGA CCAACTGTGG TGAATGCACT TACTGCAAGA ACAGAAAGAA CAGCCATCAG ATCTGTAAGA AAAGAAAATG TGAGGAGCTG AAAAAGAAAC CATCTGTTGT TGTGCCTCTG GAGGTTATAA AGGAAAACAA GAGGCCCCAG AGGGAAAAGA AGCCCAAAGT TTTAAAGGCA GATTTTGACA ACAAACCAGT AAATGGCCCC AAGTCAGAAT CCATGGACTA CAGTAGATGT GGTCATGGGG AAGAACAAAA ATTGGAATTG AACCCACATA CTGTTGAAAA TGTAACTAAA AATGAAGACA GCATGACAGG CATCGAGGTG GAGAAGTGGA CACAAAACAA GAAATCACAG TTAACTGATC ACGTGAAAGG AGATTTTAGT GCTAATGTCC CAGAAGCTGA AAAATCGAAA AACTCTGAAG TTGACAAGAA ACGAACCAAA TCTCCAAAAT TGTTTGTACA AACCGTAAGA AATGGCATTA AACATGTACA CTGTTTACCA GCTGAAACAA ATGTTTCATT TAAAAAATTC AATATTGAAG AATTCGGCAA GACATTGGAA AACAATTCTT ATAAATTCCT AAAAGACACT GCAAACCATA AAAACGCTAT GAGCTCTGTT GCTACTGATA TGAGTTGTGA TCATCTCAAG GGGAGAAGTA ACGTTTTAGT ATTCCAGCAG CCTGGCTTTA ACTGCAGTTC CATTCCACAT TCTTCACACT CCATCATAAA TCATCATGCT AGTATACACA ATGAAGGTGA TCAACCAAAA ACTCCTGAGA ATATACCAAG TAAAGAACCA AAAGATGGAT CTCCCGTTCA ACCAAGTCTC TTATCGTTAA TGAAAGATAG GAGATTAACA TTGGAGCAAG TGGTAGCCAT AGAGGCCCTG ACTCAACTCT CAGAAGCCCC ATCAGAGAAT TCCTCCCCAT CAAAGTCAGA GAAGGATGAG GAATCAGAGC AGAGAACAGC CAGTTTGCTT AATAGCTGCA AAGCTATCCT CTACACTGTA AGAAAAGACC TCCAAGACCC AAACTTACAG GGAGAGCCAC CAAAACTTAA TCACTGTCCA TCTTTGGAAA AACAAAGTTC ATGCAACACG GTGGTTTTCA ATGGGCAAAC TACTACCCTT TCCAACTCAC ATATCAACTC AGCTACTAAC CAAGCATCCA CAAAGTCACA TGAATATTCA AAAGTCACAA ATTCATTATC TCTTTTTATA CCAAAATCAA ATTCATCCAA GATTGACACC AATAAAAGTA TTGCTCAAGG GATAATTACT CTTGACAATT GTTCCAATGA TTTGCATCAG TTGCCACCAA GAAATAATGA AGTGGAGTAT TGCAACCAGT TACTGGACAG CAGCAAAAAA TTGGACTCAG ATGATCTATC ATGTCAGGAT GCAACCCATA CCCAAATTGA GGAAGATGTT GCAACACAGT TGACACAACT TGCTTCGATA ATTAAGATCA ATTATATAAA ACCAGAGGAC AAAAAAGTTG AAAGTACACC AACAAGCCTT GTCACATGTA ATGTACAGCA AAAATACAAT CAGGAGAAGG GCACAATACA ACAGAAACCA CCTTCAAGTG TACACAATAA TCATGGTTCA TCATTAACAA AACAAAAGAA CCCAACCCAG AAAAAGACAA AATCCACCCC ATCAAGAGAT CGGCGGAAAA AGAAGCCCAC AGTTGTAAGT TATCAAGAAA ATGATCGGCA GAAGTGGGAA AAGTTGTCCT ATATGTATGG CACAATATGC GACATTTGGA TAGCATCGAA ATTTCAAAAT TTTGGGCAAT TTTGTCCACA TGATTTTCCT ACTGTATTTG GGAAAATTTC TTCCTCGACC AAAATATGGA AACCACTGGC TCAAACGAGG TCCATTATGC AACCCAAAAC AGTATTTCCA CCACTCACTC AGATAAAATT ACAGAGATAT CCTGAATCAG CAGAGGAAAA GGTGAAGGTT GAACCATTGG ATTCACTCAG CTTATTTCAT CTTAAAACGG AATCCAACGG GAAGGCATTC ACTGATAAAG CTTATAATTC TCAGGTACAG TTAACGGTGA ATGCCAATCA GAAAGCCCAT CCTTTGACCC AGCCCTCCTC TCCACCTAAC CAGTGTGCTA ACGTGATGGC AGGCGATGAC CAAATACGGT TTCAGCAGGT TGTTAAGGAG CAACTCATGC ATCAGAGACT GCCAACATTG CCTGGTATCT CTCATGAAAC ACCCTTACCG GAGTCAGCAC TAACTCTCAG GAATGTAAAT GTAGTGTGTT CAGGTGGAAT TACAGTGGTT TCTACCAAAA GTGAAGAGGA AGTCTGTTCA TCCAGTTTTG GAACATCAGA ATTTTCCACA GTGGACAGTG CACAGAAAAA TTTTAATGAT TATGCCATGA ACTTCTTTAC TAACCCTACA AAAAACCTAG TGTCTATAAC TAAAGATTCT GAACTGCCCA CCTGCAGCTG TCTTGATCGA GTTATACAAA AAGACAAAGG CCCATATTAT ACACACCTTG GGGCAGGACC AAGTGTTGCT GCTGTCAGGG AAATCATGGA GAATAGGTAT GGTCAAAAAG GAAACGCAAT AAGGATAGAA ATAGTAGTGT ACACCGGTAA AGAAGGGAAA AGCTCTCATG GGTGTCCAAT TGCTAAGTGG GTTTTAAGAA GAAGCAGTGA TGAAGAAAAA GTTCTTTGTT TGGTCCGGCA GCGTACAGGC CACCACTGTC CAACTGCTGT GATGGTGGTG CTCATCATGG TGTGGGATGG CATCCCTCTT CCAATGGCCG ACCGGCTATA CACAGAGCTC ACAGAGAATC TAAAGTCATA CAATGGGCAC CCTACCGACA GAAGATGCAC CCTCAATGAA AATCGTACCT GTACATGTCA AGGAATTGAT CCAGAGACTT GTGGAGCTTC ATTCTCTTTT GGCTGTTCAT GGAGTATGTA CTTTAATGGC TGTAAGTTTG GTAGAAGCCC AAGCCCCAGA AGATTTAGAA TTGATCCAAG CTCTCCCTTA CATACCTACT ATGAAAGAAT TACTAAAGGA CGTAATCCAG AAAGAAGATA TATGAAACCG GAACGAATCA GTCCGGGACA CGAGGCCATG GAAAAAAACC TTGAAGATAA CTTACAGAGT TTGGCTACAC GATTAGCTCC AATTTATAAG CAGTATGCTC CAGTAGCTTA CCAAAATCAG GTGGAATATG AAAATGTTGC CCGAGAATGT CGGCTTGGCA GCAAGGAAGG TCGTCCCTTC TCTGGGGTCA CTGCTTGCCT GGACTTCTGT GCTCATCCCC ACAGGGACAT TCACAACATG AATAATGGAA GCACTGTGGT TTGTACCTTA ACTCGAGAAG ATAACCGCTC TTTGGGTGTT ATTCCTCAAG ATGAGCAGCT CCATGTGCTA CCTCTTTATA AGCTTTCAGA CACAGATGAG TTTGGCTCCA AGGAAGGAAT GGAAGCCAAG ATCAAATCTG GGGCCATCGA GGTCCTGGCA CCCCGCCGCA AAAAAAGAAC GTGTTTCACT CAGCCTGTTC CCCGTTCTGG AAAGAAGAGG GCTGCGATGA TGACAGAGGT TCTTGCACAT AAGATAAGGG CAGTGGAAAA GAAACCTATT CCCCGAATCA AGCGGAAGAA TAACTCAACA ACAACAAACA ACAGTAAGCC TTCGTCACTG CCAACCTTAG GGAGTAACAC TGAGACCGTG CAACCTGAAG TAAAAAGTGA AACCGAACCC CATTTTATCT TAAAAAGTTC AGACAACACT AAAACTTATT CGCTGATGCC ATCCGCTCCT CACCCAGTGA AAGAGGCATC TCCAGGCTTC TCCTGGTCCC CGAAGACTGC TTCAGCCACA CCAGCTCCAC TGAAGAATGA CGCAACAGCC TCATGCGGGT TTTCAGAAAG AAGCAGCACT CCCCACTGTA CGATGCCTTC GGGAAGACTC AGTGGTGCCA ATGCAGCTGC TGCTGATGGC CCTGGCATTT CACAGCTTGG CGAAGTGGCT CCTCTCCCCA CCCTGTCTGC TCCTGTGATG GAGCCCCTCA TTAATTCTGA GCCTTCCACT GGTGTGACTG AGCCGCTAAC GCCTCATCAG CCAAACCACC AGCCCTCCTT CCTCACCTCT CCTCAAGACC TTGCCTCTTC TCCAATGGAA GAAGATGAGC AGCATTCTGA AGCAGATGAG CCTCCATCAG ACGAACCCCT ATCTGATGAC CCCCTGTCAC CTGCTGAGGA GAAATTGCCC CACATTGATG AGTATTGGTC AGACAGTGAG CACATCTTTT TGGATGCAAA TATTGGTGGG GTGGCCATCG CACCTGCTCA CGGCTCGGTT TTGATTGAGT GTGCCCGGCG AGAGCTGCAC GCTACCACTC CTGTTGAGCA CCCCAACCGT AATCATCCAA CCCGCCTCTC CCTTGTCTTT TACCAGCACA AAAACCTAAA TAAGCCCCAA CATGGTTTTG AACTAAACAA GATTAAGTTT GAGGCTAAAG AAGCTAAGAA TAAGAAAATG AAGGCCTCAG AGCAAAAAGA CCAGGCAGCT AATGAAGGTC CAGAACAGTC CTCTGAAGTA AATGAATTGA ACCAAATTCC TTCTCATAAA GCATTAACAT TAACCCATGA CAATGTTGTC ACCGTGTCCC CTTATGCTCT CACACACGTT GCGGGGCCCT ATAACCATTG GGTCTGAGCG GCCGCTCGAG TCTAGAGGGC CCGTTTAAAC CCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCA
