pAAV-CMV-Cas9-mCerulean-U6-TNFRSF21(mouse)-sgRNA1
$70.00
In stock
SKU
PL44180
100μg liquid plasmid(Instantly):$91
| name | pAAV-CMV-Cas9-mCerulean-U6-TNFRSF21(mouse)-sgRNA1 | another name | apAAV-CMV-Cas9-mCerulean-U6-TNFRSF21(mouse)-sgRNA1 |
| Insert Size (bp) | Plasmid host | Mammary cell,Adeno-associated virus | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT AGGGGTTCCT GCGGCCTCTA GACTCGAGGC GTTGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTCTC TGGCTAACTA CCGGTGCCAC CATGGACTAT AAGGACCACG ACGGAGACTA CAAGGATCAT GATATTGATT ACAAAGACGA TGACGATAAG ATGGCCCCAA AGAAGAAGCG GAAGGTCGGT ATCCACGGAG TCCCAGCAGC CGACAAGAAG TACAGCATCG GCCTGGACAT CGGCACCAAC TCTGTGGGCT GGGCCGTGAT CACCGACGAG TACAAGGTGC CCAGCAAGAA ATTCAAGGTG CTGGGCAACA CCGACCGGCA CAGCATCAAG AAGAACCTGA TCGGAGCCCT GCTGTTCGAC AGCGGCGAAA CAGCCGAGGC CACCCGGCTG AAGAGAACCG CCAGAAGAAG ATACACCAGA CGGAAGAACC GGATCTGCTA TCTGCAAGAG ATCTTCAGCA ACGAGATGGC CAAGGTGGAC GACAGCTTCT TCCACAGACT GGAAGAGTCC TTCCTGGTGG AAGAGGATAA GAAGCACGAG CGGCACCCCA TCTTCGGCAA CATCGTGGAC GAGGTGGCCT ACCACGAGAA GTACCCCACC ATCTACCACC TGAGAAAGAA ACTGGTGGAC AGCACCGACA AGGCCGACCT GCGGCTGATC TATCTGGCCC TGGCCCACAT GATCAAGTTC CGGGGCCACT TCCTGATCGA GGGCGACCTG AACCCCGACA ACAGCGACGT GGACAAGCTG TTCATCCAGC TGGTGCAGAC CTACAACCAG CTGTTCGAGG AAAACCCCAT CAACGCCAGC GGCGTGGACG CCAAGGCCAT CCTGTCTGCC AGACTGAGCA AGAGCAGACG GCTGGAAAAT CTGATCGCCC AGCTGCCCGG CGAGAAGAAG AATGGCCTGT TCGGAAACCT GATTGCCCTG AGCCTGGGCC TGACCCCCAA CTTCAAGAGC AACTTCGACC TGGCCGAGGA TGCCAAACTG CAGCTGAGCA AGGACACCTA CGACGACGAC CTGGACAACC TGCTGGCCCA GATCGGCGAC CAGTACGCCG ACCTGTTTCT GGCCGCCAAG AACCTGTCCG ACGCCATCCT GCTGAGCGAC ATCCTGAGAG TGAACACCGA GATCACCAAG GCCCCCCTGA GCGCCTCTAT GATCAAGAGA TACGACGAGC ACCACCAGGA CCTGACCCTG CTGAAAGCTC TCGTGCGGCA GCAGCTGCCT GAGAAGTACA AAGAGATTTT CTTCGACCAG AGCAAGAACG GCTACGCCGG CTACATTGAC GGCGGAGCCA GCCAGGAAGA GTTCTACAAG TTCATCAAGC CCATCCTGGA AAAGATGGAC GGCACCGAGG AACTGCTCGT GAAGCTGAAC AGAGAGGACC TGCTGCGGAA GCAGCGGACC TTCGACAACG GCAGCATCCC CCACCAGATC CACCTGGGAG AGCTGCACGC CATTCTGCGG CGGCAGGAAG ATTTTTACCC ATTCCTGAAG GACAACCGGG AAAAGATCGA GAAGATCCTG ACCTTCCGCA TCCCCTACTA CGTGGGCCCT CTGGCCAGGG GAAACAGCAG ATTCGCCTGG ATGACCAGAA AGAGCGAGGA AACCATCACC CCCTGGAACT TCGAGGAAGT GGTGGACAAG GGCGCTTCCG CCCAGAGCTT CATCGAGCGG ATGACCAACT TCGATAAGAA CCTGCCCAAC GAGAAGGTGC TGCCCAAGCA CAGCCTGCTG TACGAGTACT TCACCGTGTA TAACGAGCTG ACCAAAGTGA AATACGTGAC CGAGGGAATG AGAAAGCCCG CCTTCCTGAG CGGCGAGCAG AAAAAGGCCA TCGTGGACCT GCTGTTCAAG ACCAACCGGA AAGTGACCGT GAAGCAGCTG AAAGAGGACT ACTTCAAGAA AATCGAGTGC TTCGACTCCG TGGAAATCTC CGGCGTGGAA GATCGGTTCA ACGCCTCCCT GGGCACATAC CACGATCTGC TGAAAATTAT CAAGGACAAG GACTTCCTGG ACAATGAGGA AAACGAGGAC ATTCTGGAAG ATATCGTGCT GACCCTGACA CTGTTTGAGG ACAGAGAGAT GATCGAGGAA CGGCTGAAAA CCTATGCCCA CCTGTTCGAC GACAAAGTGA TGAAGCAGCT GAAGCGGCGG AGATACACCG GCTGGGGCAG GCTGAGCCGG AAGCTGATCA ACGGCATCCG GGACAAGCAG TCCGGCAAGA CAATCCTGGA TTTCCTGAAG TCCGACGGCT TCGCCAACAG AAACTTCATG CAGCTGATCC ACGACGACAG CCTGACCTTT AAAGAGGACA TCCAGAAAGC CCAGGTGTCC GGCCAGGGCG ATAGCCTGCA CGAGCACATT GCCAATCTGG CCGGCAGCCC CGCCATTAAG AAGGGCATCC TGCAGACAGT GAAGGTGGTG GACGAGCTCG TGAAAGTGAT GGGCCGGCAC AAGCCCGAGA ACATCGTGAT CGAAATGGCC AGAGAGAACC AGACCACCCA GAAGGGACAG AAGAACAGCC GCGAGAGAAT GAAGCGGATC GAAGAGGGCA TCAAAGAGCT GGGCAGCCAG ATCCTGAAAG AACACCCCGT GGAAAACACC CAGCTGCAGA ACGAGAAGCT GTACCTGTAC TACCTGCAGA ATGGGCGGGA TATGTACGTG GACCAGGAAC TGGACATCAA CCGGCTGTCC GACTACGATG TGGACCATAT CGTGCCTCAG AGCTTTCTGA AGGACGACTC CATCGACAAC AAGGTGCTGA CCAGAAGCGA CAAGAACCGG GGCAAGAGCG ACAACGTGCC CTCCGAAGAG GTCGTGAAGA AGATGAAGAA CTACTGGCGG CAGCTGCTGA ACGCCAAGCT GATTACCCAG AGAAAGTTCG ACAATCTGAC CAAGGCCGAG AGAGGCGGCC TGAGCGAACT GGATAAGGCC GGCTTCATCA AGAGACAGCT GGTGGAAACC CGGCAGATCA CAAAGCACGT GGCACAGATC CTGGACTCCC GGATGAACAC TAAGTACGAC GAGAATGACA AGCTGATCCG GGAAGTGAAA GTGATCACCC TGAAGTCCAA GCTGGTGTCC GATTTCCGGA AGGATTTCCA GTTTTACAAA GTGCGCGAGA TCAACAACTA CCACCACGCC CACGACGCCT ACCTGAACGC CGTCGTGGGA ACCGCCCTGA TCAAAAAGTA CCCTAAGCTG GAAAGCGAGT TCGTGTACGG CGACTACAAG GTGTACGACG TGCGGAAGAT GATCGCCAAG AGCGAGCAGG AAATCGGCAA GGCTACCGCC AAGTACTTCT TCTACAGCAA CATCATGAAC TTTTTCAAGA CCGAGATTAC CCTGGCCAAC GGCGAGATCC GGAAGCGGCC TCTGATCGAG ACAAACGGCG AAACCGGGGA GATCGTGTGG GATAAGGGCC GGGATTTTGC CACCGTGCGG AAAGTGCTGA GCATGCCCCA AGTGAATATC GTGAAAAAGA CCGAGGTGCA GACAGGCGGC TTCAGCAAAG AGTCTATCCT GCCCAAGAGG AACAGCGATA AGCTGATCGC CAGAAAGAAG GACTGGGACC CTAAGAAGTA CGGCGGCTTC GACAGCCCCA CCGTGGCCTA TTCTGTGCTG GTGGTGGCCA AAGTGGAAAA GGGCAAGTCC AAGAAACTGA AGAGTGTGAA AGAGCTGCTG GGGATCACCA TCATGGAAAG AAGCAGCTTC GAGAAGAATC CCATCGACTT TCTGGAAGCC AAGGGCTACA AAGAAGTGAA AAAGGACCTG ATCATCAAGC TGCCTAAGTA CTCCCTGTTC GAGCTGGAAA ACGGCCGGAA GAGAATGCTG GCCTCTGCCG GCGAACTGCA GAAGGGAAAC GAACTGGCCC TGCCCTCCAA ATATGTGAAC TTCCTGTACC TGGCCAGCCA CTATGAGAAG CTGAAGGGCT CCCCCGAGGA TAATGAGCAG AAACAGCTGT TTGTGGAACA GCACAAGCAC TACCTGGACG AGATCATCGA GCAGATCAGC GAGTTCTCCA AGAGAGTGAT CCTGGCCGAC GCTAATCTGG ACAAAGTGCT GTCCGCCTAC AACAAGCACC GGGATAAGCC CATCAGAGAG CAGGCCGAGA ATATCATCCA CCTGTTTACC CTGACCAATC TGGGAGCCCC TGCCGCCTTC AAGTACTTTG ACACCACCAT CGACCGGAAG AGGTACACCA GCACCAAAGA GGTGCTGGAC GCCACCCTGA TCCACCAGAG CATCACCGGC CTGTACGAGA CACGGATCGA CCTGTCTCAG CTGGGAGGCG ACAAAAGGCC GGCGGCCACG AAAAAGGCCG GCCAGGCAAA AAAGAAAAAG GAGTTCGGCA GTGGAGAGGG CAGAGGAAGT CTGCTAACAT GCGGTGACGT CGAGGAGAAT CCTGGCCCAA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTGG GGCGTGCAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACGCCATC AGCGACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTGAA TTCCTAGAGC TCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGAGAATAG CAGGCATGCT GGGGAGGTAC CTGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG GCTGTTAGAG AGATAATTGG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAACAC CGAGATCATG AACGGGCCTA CGGTTTTAGT ACTCTGGAAA CAGAATCTAC TAAAACAAGG CAAAATGCCG TGTTTATCTC GTCAACTTGT TGGCGAGATT TTTGCGGCCG CAGGAACCCC TAGTGATGGA GTTGGCCACT CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC CCGACGCCCG GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCTGCCTGCA GGGGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATACG TCAAAGCAAC CATAGTACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTTGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGGC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGTTTACAAT TTTATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGAAGCCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGT
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