NM_053025.4(1synonymous mutations );AAT7; KRP; MLCK; MLCK1; MLCK108; MLCK210;MMIHS; MMIHS1; MSTP083; MYLK-L; MYLK1; smMLCK
100μg liquid plasmid(Instantly):$149
| name | pLV2-CMV-EGFP-MYLK(human)(1synonymous mutations)-Puro | another name | apLV2-CMV-EGFP-MYLK(human)(1synonymous mutations)-Puro |
| Insert Size (bp) | 5745bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAGA TCCAGTTTAT CGATAAGCTT GGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ACACCGACTC TACTAGAGGA TCGCTAGCGC TACCGGACTC AGATCTCGAG CTCAAGCTTC GAATTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG CTGTACAAGT CCGGACTCAG ATCTCGAGGC GGCGGAGGGA GTGGAGGAGG GGGAAGCGGA GGCGGAGGGA GTGGATCCAT GGGGGATGTG AAGCTGGTTG CCTCGTCACA CATTTCCAAA ACCTCCCTCA GTGTGGATCC CTCAAGAGTT GACTCCATGC CCCTGACAGA GGCCCCTGCT TTCATTTTGC CCCCTCGGAA CCTCTGCATC AAAGAAGGAG CCACCGCCAA GTTCGAAGGG CGGGTCCGGG GTTACCCAGA GCCCCAGGTG ACATGGCACA GAAACGGGCA ACCCATCACC AGCGGGGGCC GCTTCCTGCT GGATTGCGGC ATCCGGGGGA CTTTCAGCCT TGTGATTCAT GCTGTCCATG AGGAGGACAG GGGAAAGTAT ACCTGTGAAG CCACCAATGG CAGTGGTGCT CGCCAGGTGA CAGTGGAGTT GACAGTAGAA GGAAGTTTTG CGAAGCAGCT TGGTCAGCCT GTTGTTTCCA AAACCTTAGG GGATAGATTT TCAGCTCCAG CAGTGGAGAC CCGTCCTAGC ATCTGGGGGG AGTGCCCACC AAAGTTTGCT ACCAAGCTGG GCCGAGTTGT GGTCAAAGAA GGACAGATGG GACGATTCTC CTGCAAGATC ACTGGCCGGC CCCAACCGCA GGTCACCTGG CTCAAGGGAA ATGTTCCACT GCAGCCGAGT GCCCGTGTGT CTGTGTCTGA GAAGAACGGC ATGCAGGTTC TGGAAATCCA TGGAGTCAAC CAAGATGACG TGGGAGTGTA CACGTGCCTG GTGGTGAACG GGTCGGGGAA GGCCTCGATG TCAGCTGAAC TTTCCATCCA AGGTTTGGAC AGTGCCAATA GGTCATTTGT GAGAGAAACA AAAGCCACCA ATTCAGATGT CAGGAAAGAG GTGACCAATG TAATCTCAAA GGAGTCGAAG CTGGACAGTC TGGAGGCTGC AGCCAAAAGC AAGAACTGCT CCAGCCCCCA GAGAGGTGGC TCCCCACCCT GGGCTGCAAA CAGCCAGCCT CAGCCCCCAA GGGAGTCCAA GCTGGAGTCA TGCAAGGACT CGCCCAGAAC GGCCCCGCAG ACCCCGGTCC TTCAGAAGAC TTCCAGCTCC ATCACCCTGC AGGCCGCAAG AGTTCAGCCG GAACCAAGAG CACCAGGCCT GGGGGTCCTA TCACCTTCTG GAGAAGAGAG GAAGAGGCCA GCTCCTCCCC GTCCAGCCAC CTTCCCCACC AGGCAGCCTG GCCTGGGGAG CCAAGATGTT GTGAGCAAGG CTGCTAACAG GAGAATCCCC ATGGAGGGCC AGAGGGATTC AGCATTCCCC AAATTTGAGA GCAAGCCCCA AAGCCAGGAG GTCAAGGAAA ATCAAACTGT CAAGTTCAGA TGTGAAGTTT CCGGGATTCC AAAGCCTGAA GTGGCCTGGT TCCTGGAAGG CACCCCCGTG AGGAGACAGG AAGGCAGCAT TGAGGTTTAT GAAGATGCTG GCTCCCATTA CCTCTGCCTG CTGAAAGCCC GGACCAGGGA CAGTGGGACA TACAGCTGCA CTGCTTCCAA CGCCCAAGGC CAGCTGTCCT GTAGCTGGAC CCTCCAAGTG GAAAGGCTTG CCGTGATGGA GGTGGCCCCC TCCTTCTCCA GTGTCCTGAA GGACTGCGCT GTTATTGAGG GCCAGGATTT TGTGCTGCAG TGCTCCGTAC GGGGGACCCC AGTGCCCCGG ATCACTTGGC TGCTGAATGG GCAGCCCATC CAGTACGCTC GCTCCACCTG CGAGGCCGGC GTGGCTGAGC TCCACATCCA GGATGCCCTG CCGGAGGACC ATGGCACCTA CACCTGCCTA GCTGAGAATG CCTTGGGGCA GGTGTCCTGC AGCGCCTGGG TCACCGTCCA TGAAAAGAAG AGTAGCAGGA AGAGTGAGTA CCTTCTGCCT GTGGCTCCCA GCAAGCCCAC TGCACCCATC TTCCTGCAGG GCCTCTCTGA TCTCAAAGTC ATGGATGGAA GCCAGGTCAC TATGACTGTC CAAGTGTCAG GGAATCCACC CCCTGAAGTC ATCTGGCTGC ACAATGGGAA TGAGATCCAA GAGTCAGAGG ACTTCCACTT TGAACAGAGA GGAACTCAGC ACAGCCTTTG TATCCAGGAA GTGTTCCCGG AGGACACGGG CACGTACACC TGCGAGGCCT GGAACAGCGC TGGAGAGGTC CGCACCCAGG CCGTGCTCAC GGTACAAGAG CCTCACGATG GCACCCAGCC CTGGTTCATC AGTAAGCCTC GCTCAGTGAC AGCCTCCCTG GGCCAGAGTG TCCTCATCTC CTGCGCCATA GCTGGTGACC CCTTTCCTAC CGTGCACTGG CTCAGAGATG GCAAAGCCCT CTGCAAAGAC ACTGGCCACT TCGAGGTGCT TCAGAATGAG GACGTGTTCA CCCTGGTTCT AAAGAAGGTG CAGCCCTGGC ATGCCGGCCA GTATGAGATC CTGCTCAAGA ACCGGGTTGG CGAATGCAGT TGCCAGGTGT CACTGATGCT ACAGAACAGC TCTGCCAGAG CCCTTCCACG GGGGAGGGAG CCTGCCAGCT GCGAGGACCT CTGTGGTGGA GGAGTTGGTG CTGATGGTGG TGGTAGTGAC CGCTATGGGT CCCTGAGGCC TGGCTGGCCA GCAAGAGGGC AGGGTTGGCT AGAGGAGGAA GACGGCGAGG ACGTGCGAGG GGTGCTGAAG AGGCGCGTGG AGACGAGGCA GCACACTGAG GAGGCGATCC GCCAGCAGGA GGTGGAGCAG CTGGACTTCC GAGACCTCCT GGGGAAGAAG GTGAGTACAA AGACCCTATC GGAAGACGAC CTGAAGGAGA TCCCAGCCGA GCAGATGGAT TTCCGTGCCA ACCTGCAGCG GCAAGTGAAG CCAAAGACTG TGTCTGAGGA AGAGAGGAAG GTGCACAGCC CCCAGCAGGT CGATTTTCGC TCTGTCCTGG CCAAGAAGGG GACTTCCAAG ACCCCCGTGC CTGAGAAGGT GCCACCGCCA AAACCTGCCA CCCCGGATTT TCGCTCAGTG CTGGGTGGCA AGAAGAAATT ACCAGCAGAG AATGGCAGCA GCAGTGCCGA GACCCTGAAT GCCAAGGCAG TGGAGAGTTC CAAGCCCCTG AGCAATGCAC AGCCTTCAGG GCCCTTGAAA CCCGTGGGCA ACGCCAAGCC TGCTGAGACC CTGAAGCCAA TGGGCAACGC CAAGCCTGCC GAGACCCTGA AGCCCATGGG CAATGCCAAG CCTGATGAGA ACCTGAAATC CGCTAGCAAA GAAGAACTCA AGAAAGACGT TAAGAATGAT GTGAACTGCA AGAGAGGCCA TGCAGGGACC ACAGATAATG AAAAGAGATC AGAGAGCCAG GGGACAGCCC CAGCCTTCAA GCAGAAGCTG CAAGATGTTC ATGTGGCAGA GGGCAAGAAG CTGCTGCTCC AGTGCCAGGT GTCTTCTGAC CCCCCAGCCA CCATCATCTG GACGCTGAAC GGAAAGACCC TCAAGACCAC CAAGTTCATC ATCCTCTCCC AGGAAGGCTC ACTCTGCTCC GTCTCCATCG AGAAGGCACT GCCTGAGGAC AGAGGCTTAT ACAAGTGTGT AGCCAAGAAT GACGCTGGCC AGGCGGAGTG CTCCTGCCAA GTCACCGTGG ATGATGCTCC AGCCAGTGAG AACACCAAGG CCCCAGAGAT GAAATCCCGG AGGCCCAAGA GCTCTCTTCC TCCCGTGCTA GGAACTGAGA GTGATGCGAC TGTGAAAAAG AAACCTGCCC CCAAGACACC TCCGAAGGCA GCAATGCCCC CTCAGATCAT CCAGTTCCCT GAGGACCAGA AGGTACGCGC AGGAGAGTCA GTGGAGCTGT TTGGCAAAGT GACAGGCACT CAGCCCATCA CCTGTACCTG GATGAAGTTC CGAAAGCAGA TCCAGGAAAG CGAGCACATG AAGGTGGAGA ACAGCGAGAA TGGCAGCAAG CTCACCATCC TGGCCGCGCG CCAGGAGCAC TGCGGCTGCT ACACACTGCT GGTGGAGAAC AAGCTGGGCA GCAGGCAGGC CCAGGTCAAC CTCACTGTCG TGGATAAGCC AGACCCCCCA GCTGGCACAC CTTGTGCCTC TGACATTCGG AGCTCCTCAC TGACCCTGTC CTGGTATGGC TCCTCATATG ATGGGGGCAG TGCTGTACAG TCCTACAGCA TCGAGATCTG GGACTCAGCC AACAAGACGT GGAAGGAACT AGCCACATGC CGCAGCACCT CTTTCAACGT CCAGGACCTG CTGCCTGACC ACGAATATAA GTTCCGTGTA CGTGCAATCA ACGTGTATGG AACCAGTGAG CCAAGCCAGG AGTCTGAACT CACAACGGTA GGAGAGAAAC CTGAAGAGCC GAAGGATGAA GTGGAGGTGT CAGATGATGA TGAGAAGGAG CCCGAGGTTG ATTACCGGAC AGTGACAATC AATACTGAAC AAAAAGTATC TGACTTCTAC GACATTGAGG AGAGATTAGG ATCTGGGAAA TTTGGACAGG TCTTTCGACT TGTAGAAAAG AAAACTCGAA AAGTCTGGGC AGGGAAGTTC TTCAAGGCAT ATTCAGCAAA AGAGAAAGAG AATATCCGGC AGGAGATTAG CATCATGAAC TGCCTCCACC ACCCTAAGCT GGTCCAGTGT GTGGATGCCT TTGAAGAAAA GGCCAACATC GTCATGGTCC TGGAGATCGT GTCAGGAGGG GAGCTGTTTG AGCGCATCAT TGACGAGGAC TTTGAGCTGA CGGAGCGTGA GTGCATCAAG TACATGCGGC AGATCTCGGA GGGAGTGGAG TACATCCACA AGCAGGGCAT CGTGCACCTG GACCTCAAGC CGGAGAACAT CATGTGTGTC AACAAGACGG GCACCAGGAT CAAGCTCATC GACTTTGGTC TGGCCAGGAG GCTGGAGAAT GCGGGGTCTC TGAAGGTCCT CTTTGGCACC CCAGAATTTG TGGCTCCTGA AGTGATCAAC TATGAGCCCA TCGGCTACGC CACAGACATG TGGAGCATCG GGGTCATCTG CTACATCCTA GTCAGTGGCC TTTCCCCCTT CATGGGAGAC AACGATAACG AAACCTTGGC CAACGTTACC TCAGCCACCT GGGACTTCGA CGACGAGGCA TTCGATGAGA TCTCCGACGA TGCCAAGGAT TTCATCAGCA ATCTGCTGAA GAAAGATATG AAAAACCGCC TGGACTGCAC GCAGTGCCTT CAGCATCCAT GGCTAATGAA AGATACCAAG AACATGGAGG CCAAGAAACT CTCCAAGGAC CGGATGAAGA AGTACATGGC AAGAAGGAAA TGGCAGAAAA CGGGCAATGC TGTGAGAGCC ATTGGAAGAC TGTCCTCTAT GGCAATGATC TCAGGGCTCA GTGGCAGGAA ATCCTCAACA GGGTCACCAA CCAGCCCGCT CAATGCAGAA AAACTAGAAT CTGAAGAAGA TGTGTCCCAA GCTTTCCTTG AGGCTGTTGC TGAGGAAAAG CCTCATGTAA AACCCTATTT CTCTAAGACC ATTCGCGATT TAGAAGTTGT GGAGGGAAGT GCTGCTAGAT TTGACTGCAA GATTGAAGGA TACCCAGACC CCGAGGTTGT CTGGTTCAAA GATGACCAGT CAATCAGGGA GTCCCGCCAC TTCCAGATAG ACTACGATGA GGACGGGAAC TGCTCTTTAA TTATTAGTGA TGTTTGCGGG GATGACGATG CCAAGTACAC CTGCAAGGCT GTCAACAGTC TTGGAGAAGC CACCTGCACA GCAGAGCTCA TTGTGGAAAC GATGGAGGAA GGTGAAGGGG AAGGGGAAGA GGAAGAAGAG TGATCTAGAT AATTCTACCG GGTAGGGGAG GCGCTTTTCC CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG CACTTGGCGC TACACAAGTG GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG CCAACCGGCT CCGTTCTTTG GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG GAAGTTCCCC CCCGCCCCGC AGCTCGCGTC GTGCAGGACG TGACAAATGG AAGTAGCACG TCTCACTAGT CTCGTGCAGA TGGACAGCAC CGCTGAGCAA TGGAAGCGGG TAGGCCTTTG GGGCAGCGGC CAATAGCAGC TTTGCTCCTT CGCTTTCTGG GCTCAGAGGC TGGGAAGGGG TGGGTCCGGG GGCGGGCTCA GGGGCGGGCT CAGGGGCGGG GCGGGCGCCC GAAGGTCCTC CGGAGGCCCG GCATTCTGCA CGCTTCAAAA GCGCACGTCT GCCGCGCTGT TCTCCTCTTC CTCATCTCCG GGCCTTTCGA CCTGCAGCCC AAGCTTACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAC CGCGTCTGGA ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CCTGGAATTA ATTCTGCAGT CGAGACCTAG AAAAACATGG AGCAATCACA AGTAGCAATA CAGCAGCTAC CAATGCTGAT TGTGCCTGGC TAGAAGCACA AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC ACCTCAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT AAAAGAAAAG AGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATATCCTTGA TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTAG CAGAACTACA CACCAGGGCC AGGGGTCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG CTAGTACCAG TTGAGCCAGA TAAGGTAGAA GAGGCCAATA AAGGAGAGAA CACCAGCTTG TTACACCCTG TGAGCCTGCA TGGGATGGAT GACCCGGAGA GAGAAGTGTT AGAGTGGAGG TTTGACAGCC GCCTAGCATT TCATCACGTG GCCCGAGAGC TGCATCCGGA GTACTTCAAG AACTGCTGAT ATCGAGCTTG CTACAAGGGA CTTTCCGCTG GGGACTTTCC AGGGAGGCGT GGCCTGGGCG GGACTGGGGA GTGGCGAGCC CTCAGATCCT GCATATAAGC AGCTGCTTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTAGTAGTT CATGTCATCT TATTATTCAG TATTTATAAC TTGCAAAGAA ATGAATATCA GAGAGTGAGA GGCCTTGACA TTGCTAGCGT TTTACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCAA CTGGATAACT CAAGCTAACC AAAATCATCC CAAACTTCCC ACCCCATACC CTATTACCAC TGCCAATTAC CTGTGGTTTC ATTTACTCTA AACCTGTGAT TCCTCTGAAT TATTTTCATT TTAAAGAAAT TGTATTTGTT AAATATGTAC TACAAACTTA GTAGTTTTTA AAGAAATTGT ATTTGTTAAA TATGTACTAC AAACTTAGTA GT
