pLV2-SP-Dusp8(mouse)-3×FLAG-P2A-mCherry
$70.00
In stock
SKU
PL44091
NM_008748.3;5530400B01Rik; Nttp1
100μg liquid plasmid(Instantly):$91
| name | pLV2-SP-Dusp8(mouse)-3×FLAG-P2A-mCherry | another name | apLV2-SP-Dusp8(mouse)-3×FLAG-P2A-mCherry |
| Insert Size (bp) | 1992bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Red | |
| Promoter | SP-107 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AAAGAGATTG CAACCCCCAG AAAGATGACA AGATTTTGGA TTAGTAATGG CAAGTTTGGA ATGGTTAACA TACAAATGGC TGGGTATTAA AATTTTCATA ATGTTGTAGG GGGCTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGCCT TTAAAAGAAA AGGGGGGATT GGGGGGTACA GTGCAGGGGA AAGAATAGTA GACATAATAG CAACAGACAT ACAAACTAAA GAATTACAAA AACAAATTAC AAAAATTCAA AATTTTCGGG TTTATTACAG GGACAGCAGA GATCCAGTTT ACTAGTGAAT TCAAGCGACT TCCTCTTTCC AGCAGAAAAG GAGAAGTAGG AGCCAAGATT TCCAAACTCT GTGGTTGCCT TGAAGCGACT TCCTCTTTCC AGAAGCGACT TCCTCTTTCC AGCAGAAAAG GAGAAGTAGG AGCTCCTACT TCTCCTTTTC TGCCAAGATT TCCAAACTCT GTGGTTGCCT TGCTCCTACT TCTCCTTTTC TGCAGAAAAG GAGAAGTAGG AGCTGGAAAG AGGAAGTCGC TTTACCCTCC CTGCCCCCTG TCCCGACCGC GACAAAAGCG ACTTCCTCTT TCCAGTGCAT TTAAGGCGCA GCCTGGAAGT GCCAGGGAGC ACTGGAGGCC ACCCAGTCGC TAGCACCGGT CGTTGGCGCG CCGCCACCAT GGCTGGGGAT CGGCTCCCGA GGAAGGTGAT GGACGCAAAG AAACTGGCCA GCCTGCTGCG TGGCGGGCCT GGGGGACCCT TGGTCATCGA CAGCCGGTCC TTCGTGGAGT ATAACAGCTG CCACGTGCTG AGCTCTGTGA ATATCTGCTG TTCAAAGCTG GTGAAGCGGC GCCTTCAGCA GGGAAAAGTG ACAATTGCTG AGCTTATCCA GCCTGCTACA CGGAGCCAGG TGGATGCCAC AGAACCACAG GATGTAGTGG TGTATGACCA GAGCACACGA GATGCCAGCG TGCTGGCAGC AGACAGCTTC CTGTCCATCC TGCTCAGCAA GCTGGACGGC TGCTTCGACA GTGTGGCCAT CCTCACAGGA GGCTTCGCCA CCTTCTCCTC CTGCTTCCCT GGCCTCTGTG AGGGCAAGCC TGCCACTCTA CCGTCCATGA GCCTCTCTCA GCCCTGCCTG CCTGTGCCCA GTGTTGGCCT GACCCGAATC CTGCCTCACC TCTACCTGGG CTCTCAGAAA GATGTCTTGA ACAAGGATCT GATGACCCAA AACGGAATAA GCTATGTCCT CAATGCCAGC AACTCCTGCC CTAAACCGGA CTTCATCTGT GAGAGCCGTT TCATGCGTAT CCCCATCAAT GACAACTACT GTGAAAAGCT GCTGCCCTGG CTGGACAAGT CCATCGAGTT TATTGATAAA GCCAAGCTGT CCAGCTGCCA AGTCATTGTT CACTGTCTGG CTGGCATCTC TCGCTCTGCC ACCATTGCCA TCGCGTACAT CATGAAAACC ATGGGCATGT CTTCTGACGA CGCATACAGG TTTGTGAAGG ATCGGCGCCC CTCCATCTCG CCCAACTTCA ACTTCCTGGG CCAGTTGCTG GAGTATGAGA GGAGTCTGAA GCTGCTGGCT GCCCTGCAGA CTGATGGACC TCACTTGGGG ACCCCTGAGC CCCTCATGGG CCCGGCAGCA GGCATCCCAC TGCCCCGGCT GCCACCATCT ACCTCAGAGA GCGCTGCCAC TGGGAGCGAG GCAGCCACCG CAGCCAGGGA GGGCAGCCCA AGTGCTGGAG GGGATGCTCC GATCCCCAGC ACAGCTCCAG CCACCAGCGC GCTGCAGCAG GGCCTGCGTG GCCTGCACCT CTCCTCTGAC CGCCTCCAGG ACACCAACCG CCTCAAGCGT TCCTTTTCCC TGGACATCAA GTCGGCCTAT GCACCCAGCA GGAGGCCCGA CTTTCCCGGC CCACCCGACC CCGGTGAAGC CCCGAAGCTC TGCAAGCTGG ACAGCCCGTC TGGGGGCACA CTGGGCCTGC CCTCGCCCAG CCCAGACAGC CCGGACTCCG TTCCAGAGTG CCGCCCACGA CCCCGCCGGC GACGCCCCCC GGCTAGTTCG CCTGCCCGCT CCCCCGCGCA TGGTCTGGGC CTGAACTTTG GAGACACGGC CCGGCAGACT CCACGGCACG GCCTCTCGGC CCTGTCGGCG CCCGGGCTGC CTGGCCCTGG CCAGCCGGCT GGCCCCGGGG GCTGGGTGCC GCCACTGGAC TCCCCAGGCA CACCGTCGCC CGACGGCCCC TGGTGCTTCA GCCCCGAGGG CGCGCAGGGT CCAGGCGCTG TGTTCTCCGC CTTTGGCCGG GTAAGTGCAG GCGCACCTGG ACCCGGTAAC AGCAGCAGCA GCGGTGGTGG TGGTGGTGGT GGTGGCGGCG GCGGCGGCGG CGGCGGCGGC GGCGGCAGCA GCAGCAGCAA CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGTAGTAGTA GTAGTAGTGA CCTGCGGAGG CGGGATGTGC GGACCGGCTG GCCCGAGGAG CCTGCTGCAG ATGCACAGTT CAAGAGGCGC AGCTGCCAGA TGGAGTTCGA AGAGGGCATG GTGGAGGGGC GGGCACGTGG CGAGGAGCTG GCAGCCCTGG GCAAGCAAAC CAGCTTCTCT GGCAGCGTGG AGGTCATCGA AGTATCGCTC GAGTCTAGAG GGCCCTTCGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGCGGATC GCTACTAACT TCAGCCTGCT GAAGCAGGCT GGAGACGTGG AGGAGAACCC TGGACCTACC GGTGCCACCA TGGTGAGCAA GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGTAG ACGCGTCTGG AACAATCAAC CTCTGGATTA CAAAATTGGA AAGATGACTA TTCAC
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