NM_007378.1;Abc10; Abcr; AW050280; D430003I15Rik; RmP
100μg liquid plasmid(Instantly):$163
| name | pLV3-CMV-Abca4(mouse)-3×FLAG-CopGFP-Puro | another name | apLV3-CMV-Abca4(mouse)-3×FLAG-CopGFP-Puro |
| Insert Size (bp) | 6933bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGG GCTTCCTCAG ACAGATACAG CTTTTGCTTT GGAAGAACTG GACTCTGAGG AAAAGGCAGA AGATTCGCTT TGTAGTGGAA CTCGTGTGGC CTTTGTCTTT GTTTTTGGTG TTAATCTGGC TGAGGAATGC CAACCCACTC TATAGTCAGC ATGAATGCCA TTTTCCCAAC AAGGCGATGC CTTCAGCAGG ACTGTTACCG TGGCTCCAGG GGATTTTCTG CAATATGAAC AACCCTTGTT TTCAAAACCC CACCCCTGGA GAGTCTCCTG GAACTGTCTC AAACTATAAC AACTCCATCT TGGCAAGAGT ATATCGAGAT TTTCAAGAAC TCTTCATGGA CACCCCGGAG GTCCAGCACC TTGGCCAGGT TTGGGCCGAG CTCCGCACCT TGTCGCAGTT CATGGACACC CTGAGGACTC ACCCTGAGAG ATTTGCAGGA AGAGGATTAC AAATCCGAGA CATCCTAAAA GATGAAGAGG CGCTGACCCT ATTTCTCATG AGAAACATTG GCCTGTCTGA CTCGGTTGCC CATCTTCTGG TCAACTCCCA AGTTCGTGTG GAGCAGTTTG CTTATGGAGT CCCAGACTTG GAACTGACAG ACATTGCCTG CAGCGAGGCC CTCCTGCAGC GCTTCATCAT CTTCAGCCAG CGTCGGGGGG CACAGACGGT ACGCGATGCC CTGTGTCCCC TCTCCCAGGT CACCCTACAG TGGATAGAAG ACACTCTGTA TGCCGATGTG GACTTCTTCA AACTCTTCCA TGTGCTCCCC ACACTCCTGG ACAGCAGTTC TCAAGGAATC AACTTGAGAT TTTGGGGAGG AATATTATCT GATCTGTCAC CAAGAATGCA AAAGTTTATC CATCGGCCAA GTGTTCAAGA CTTGCTATGG GTGAGCAGAC CTCTCCTGCA GAACGGTGGT CCTGAGACCT TCACACAGCT GATGAGCATC CTGTCTGACC TCCTGTGTGG GTACCCAGAG GGAGGAGGCT CCCGAGTGTT CTCCTTTAAC TGGTATGAAG ACAATAACTA TAAAGCCTTC CTGGGGATTG ATTCCACAAG GAAAGACCCC GCCTATTCTT ACGACAAAAG AACAACATCC TTTTGTAATT CATTGATCCA GAGCCTGGAG TCAAACCCTT TAACCAAAAT AGCCTGGAGG GCGGCAAAGC CATTGCTGAT GGGAAAAATC CTCTTTACTC CAGATTCCCC TGCTGCTCGA AGGATAATGA AGAATGCCAA CTCAACTTTT GAAGAACTGG ATCGAGTTAG GAAGTTGGTA AAAGCCTGGG AGGAAGTGGG GCCCCAGATC TGGTACTTCT TTGAGAAGAG CACACAGATG ACCGTGATCC GAGACACCCT GCAGCACCCA ACCGTCAAAG ACTTCATAAA TAGGCAGCTC GGAGAAGAAG GCATTACCAC CGAAGCCGTA TTAAACTTCT TCTCTAACGG TCCCCAAGAG AAGCAGGCTG ATGATATGAC CAGCTTTGAC TGGAGGGACA TATTCAACAT CACTGACCGA TTCCTACGCT TGGCTAATCA ATACCTGGAG TGTCTGGTCC TGGATAAGTT TGAAAGTTAT GATGATGAAG TGCAGCTCAC CCAACGAGCC CTGTCTCTCC TGGAGGAGAA CAGGTTCTGG GCCGGAGTGG TGTTCCCTGG CATGTATCCC TGGGCCAGCT CCTTACCTCC TCATGTGAAG TACAAGATTC GGATGGACAT AGATGTGGTG GAGAAGACCA ATAAGATCAA AGACAGGTAC TGGGATTCTG GTCCCAGGGC GGATCCTGTC GAAGATTTCC GGTACATCTG GGGAGGTTTT GCCTATCTAC AGGACATGGT GGAGCAAGGA ATCGTGAAGA GTCAGATGCA GGCAGAGCCT CCAATTGGAG TCTATCTCCA ACAGATGCCT TATCCCTGCT TTGTGGATGA CTCCTTCATG ATCATCCTGA ATCGCTGTTT CCCTATCTTC ATGGTGCTGG CGTGGATCTA CTCTGTCTCC ATGACCGTTA AGGGCATTGT TTTGGAGAAG GAGCTGAGGC TGAAGGAGAC CTTGAAAAAC CAAGGCGTCT CTAATGCTGT CATCTGGTGT ACCTGGTTCC TGGATAGCTT CTCCATCATG GCGCTGAGTA TCTTCCTCCT GACGCTGTTC ATCATGCATG GAAGGATCCT CCATTACAGC GATCCCTTCA TTCTCTTCCT GTTCTTGTTG GCCTTTGCCA CTGCGACCAT CATGCAGAGC TTCCTGCTCA GTACTTTGTT TTCCAAGGCC AGCCTTGCAG CAGCCTGCAG TGGGGTCATC TACTTCACCC TCTACCTACC ACACGTTCTG TGCTTTGCCT GGCAGGACCG GATGACGGCC GACCTGAAGA CGACTGTGAG CCTACTTTCT TCCGTGGCAT TTGGGTTTGG CACCGAGTAC CTGGTCCGCT TTGAGGAGCA AGGCCTGGGG CTGCAATGGA GCAACATTGG GAAGAGTCCC CTGGAAGGGG ATGAGTTCAG TTTCCTGCTG TCTATGAAGA TGATGCTTCT CGATGCTGCT CTCTACGGCT TGCTTGCTTG GTATCTTGAC CAGGTTTTCC CAGGAGACTA TGGGACCCCA CTTCCCTGGT ACTTCCTTCT GCAGGAGTCC TACTGGCTTG GTGGTGAAGG TTGTTCGACC AGAGAAGAAA GGGCTCTGGA AAAGACTGAA CCCTTAACAG AGGAGATGGA GGATCCAGAG CACCCAGAAG GAATGAATGA CTCCTTTTTT GAACGCGAGC TTCCAGGGCT GGTGCCTGGT GTGTGTGTGA AGAACCTGGT GAAGGTTTTT GAGCCCAGTG GCCGGCCAGC TGTGGACCGT CTGAACATCA CTTTCTATGA GAACCAAATC ACGGCGTTCC TGGGTCACAA CGGAGCGGGA AAGACCACCA CCTTGTCCAT CCTGACAGGA CTGTTGCCAC CGACGTCAGG AACTGTGCTC ATTGGGGGAA AAGACATTGA AACCAACCTG GATGTAGTAC GGCAGAGCCT GGGCATGTGT CCACAACACA ACATCCTGTT TCATCACCTC ACGGTGGCTG AGCACATCTT GTTCTATGCC CAGCTGAAAG GGAGATCCTG GGAGGAGGCC CAGCTTGAGA TGGAAGCCAT GCTAGAAGAC ACGGGCCTCC ACCATAAGAG GAATGAAGAA GCTCAGGACC TTTCAGGTGG CATGCAGAGG AAGCTGTCTG TTGCCATTGC TTTTGTGGGA GATTCTAAGG TGGTGGTCCT GGATGAGCCC ACCTCTGGGG TGGACCCCTA CTCCAGACGC TCCATCTGGG ACCTGCTCCT GAAGTATCGC TCAGGCAGAA CCATCATCAT GTCCACTCAC CACATGGACG AGGCAGACCT CCTTGGGGAC CGCATTGCCA TCATTTCTCA GGGAAGGCTC TACTGCTCTG GAACCCCGCT CTTCCTCAAG AACTGCTTTG GCACAGGCTT CTACTTGACC TTAGTTCGCA AGATGAAAAA CATCCAGAGC CAAAGAGGTG GCTGTGAGGG GGTCTGCAGC TGTACATCAA AGGGTTTCTC TACCAGGTGT CCAACCCGAG TCGATGAGAT AACAGAAGAA CAAGTCCTGG ATGGAGATGT GCAGGAGCTG ATGGATTTGG TATACCACCA TGTCCCAGAG GCAAAGCTGG TGGAATGCAT TGGTCAAGAA CTTATCTTCC TCCTTCCAAA CAAGAATTTC AAGCAGAGAG CATATGCCAG CCTTTTCCGA GAGCTGGAGG AGACTCTGGC TGACCTGGGG CTCAGCAGCT TTGGAATTTC TGACACTCCC CTGGAAGAGA TTTTCCTGAA GGTCACAGAG GATGCTGGAG CGGGCTCTAT GTTTGTAGGT GGCGCTCAGC AGAAAAGAGA ACAAGCCGGT CTCCGGCACC CTTGTTCGGC TCCCACTGAG AAGCTCAGGC AGTATGCCCA GGCCCCACAT ACCTGTTCCC CAGGACAAGT GGATCCTCCC AAGGGCCAAC CTTCCCCAGA GCCAGAGGAC CCCGGTGTCC CATTCAACAC AGGTGCTCGG CTGATTCTTC AACATGTGCA GGCCCTGCTA GTCAAGCGAT TCCATCACAC CATCCGCAGC CGCAAGGACT TTGTGGCTCA GATTGTTCTC CCTGCCACTT TCGTGTTCTT GGCTCTGATG CTTTCAATCA TTGTGCCTCC GTTTGGTGAA TTTCCAGCTT TGACTCTTCA TCCCTGGATG TATGGGCATC AATATACCTT CTTCAGCATG GACGAACCCA ACAATGAACA CCTTGAAGTA CTGGCAGATG TCCTTCTGAA CAGGCCAGGC TTTGGCAACC GTTGTCTAAA GGAAGAGTGG CTTCCGGAGT ACCCATGCAT TAATGCAACC TCCTGGAAGA CGCCCTCCGT GTCCCCGAAC ATCACCCACC TGTTCCAGAA GCAGAAATGG ACAGCAGCCC ACCCCTCTCC CTCCTGCAAG TGCAGCACCA GAGAGAAGCT CACCATGTTG CCCGAGTGCC CCGAGGGTGC TGGAGGGCTC CCACCCCCAC AGAGGACACA GCGCAGCACT GAAGTCCTAC AAGACCTCAC GAACAGGAAC ATCTCCGACT ACTTGGTAAA AACGTACCCT GCTCTCATAA GAAGCAGCTT AAAGAGCAAA TTCTGGGTCA ATGAACAGAG GTATGGAGGA ATTTCCATCG GAGGAAAACT CCCTGCTATC CCCATCAGCG GGGAAGCACT TGTTGGCTTT CTAAGTGGCC TTGGCCAGAT GATGAACGTG AGCGGGGGTC CTGTCACCAG GGAGGCCTCC AAAGAAATGT TAGATTTCCT CAAACATCTT GAAACCACAG ACAACATTAA GGTATGGTTT AACAACAAGG GCTGGCATGC CCTGGTCAGC TTTCTGAATG TGGCTCACAA CGCCATCTTA CGGGCCAGCC TGCCCAGGGA CAGGGACCCT GAGGAGTATG GAATCACTGT CATCAGCCAG CCTCTGAACC TGACCAAGGA GCAGCTCTCG GACATCACAG TACTGACTAC TTCCGTGGAT GCCGTGGTTG CCATATGTGT GATTTTCGCC ATGTCCTTCG TTCCAGCCAG CTTTGTCCTT TACTTGATCC AGGAGAGGGT GACGAAAGCT AAACATTTGC AGTTTATCAG TGGTGTGAGC CCCACCACCT ACTGGCTGAC CAACTTCCTC TGGGATATTA TGAATTATGC TGTGAGTGCG GGATTGGTGG TGGGCATCTT CATTGGATTT CAAAAGAAAG CCTACACATC CCCAGACAAC CTTCCTGCTC TCGTCTCTCT GCTCATGCTG TATGGATGGG CAGTCATTCC TATGATGTAT CCAGCATCCT TCCTGTTTGA AGTCCCCAGC ACAGCCTATG TGGCTTTGTC CTGTGCTAAC CTGTTCATCG GCATCAACAG CAGCGCCATC ACCTTCGTCC TGGAATTATT TGAGAATAAC CGGACGCTGC TCAGGTTCAA TGCCATGTTG AGGAAGTTGC TCATTGTCTT CCCCCACTTC TGCCTGGGCA GGGGCCTCAT TGACCTGGCA CTGAGCCAAG CTGTGACAGA CGTCTATGCC CAGTTTGGTG AGGAGTACTC TGCAAACCCA TTCCAGTGGG ACCTGATTGG GAAGAACCTG GTTGCTATGG CAATAGAAGG GGTGGTATAC TTCCTTCTGA CCCTGCTCAT CCAACACCAT TTTTTCCTCA CCCGGTGGAT CGCTGAGCCT GCTAGAGAGC CCGTTTTTGA TGAAGATGAC GATGTGGCTG AAGAAAGACA AAGAGTTATG AGTGGGGGAA ATAAAACCGA CATCTTAAAG CTAAACGAAC TAACCAAGGT TTACTCAGGC TCTTCCAGTC CAGCAGTAGA TCGGTTATGT GTTGGAGTTC GACCTGGAGA GTGCTTTGGC CTCCTGGGAG TGAATGGTGC AGGCAAAACC ACCACATTCA AGATGCTCAC TGGGGACACC ACAGTGACAT CGGGGGATGC TACTGTGGCA GGCAAGAGCA TTTTAACTAG TATTTCTGAT GTCCATCAAA ACATGGGCTA CTGTCCTCAG TTTGATGCAA TCGATGACCT GCTCACAGGC AGAGAACACC TTTACCTCTA TGCCAGGCTG CGGGGCGTGC CATCGAAGGA AATCGAGAAG GTTGCCAACT GGGGTATCCA GAGCCTGGGC TTGTCTCTCT ACGCTGACCG CCTGGCAGGC ACCTACAGTG GAGGCAATAA GAGGAAACTC TCTACAGCCA TAGCTCTCAC TGGCTGCCCT CCCCTGCTGC TGCTGGATGA GCCCACGACA GGGATGGATC CCCAGGCACG CCGCATGCTG TGGAACACCA TTGTGAGCAT CATCAGAGAA GGGAGAGCTG TGGTTCTCAC CTCCCACAGC ATGGAAGAAT GTGAAGCTCT GTGTACGCGG CTGGCCATCA TGGTGAAGGG CACCTTTCAG TGTCTGGGTA CCATCCAACA CCTCAAGTAC AAGTTTGGAG ACGGCTACAT TGTCACAATG AAAATCAAAT CTCCAAAGGA CGACTTGCTT CCCGATTTGA ATCCTGTGGA GCAGTTCTTC CAGGGCAACT TCCCTGGCAG CGTGCAGAGG GAGAGACACC ACAGCATGCT CCAGTTCCAG GTCCCCTCGT CCTCCCTGGC CAGGATCTTC CAGCTGCTCA TTTCCCACAA GGACAGCCTG CTCATCGAGG AGTACTCAGT CACCCAGACC ACGCTGGACC AGGTGTTTGT AAACTTCGCT AAACAGCAGA CTGAGACCTA TGACCTCCCT CTGCACCCCC GGGCTGCTGG AGCCAGCTGG CAAGCCAAGC TTGAAGAGAA ATCCGGACGG CTGCAGACAC AGGAGCCTTT GCCCGCAGGA TCTGAACAAC TGGCTAACGG AAGCAACCCC ACGGCAGCAG AAGATAAGCA CACCAGGAGT CCACAGGGAT CCGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTA GCGGCCGCGA AGGATCTGCG ATCGCTCCGG TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT CGGCAATTGA ACGGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGCTGAAGC TTCGAGGGGC TCGCATCTCT CCTTCACGCG CCCGCCGCCC TACCTGAGGC CGCCATCCAC GCCGGTTGAG TCGCGTTCTG CCGCCTCCCG CCTGTGGTGC CTCCTGAACT GCGTCCGCCG TCTAGGTAAG TTTAAAGCTC AGGTCGAGAC CGGGCCTTTG TCCGGCGCTC CCTTGGAGCC TACCTAGACT CAGCCGGCTC TCCACGCTTT GCCTGACCCT GCTTGCTCAA CTCTACGTCT TTGTTTCGTT TTCTGTTCTG CGCCGTTACA GATCCAAGCT GTGACCGGCG CCTACGCTAG ACGCCACCAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCAAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGGTG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGTGCTGG TGGGCAGCTT CGCCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TTCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGCTGC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCCATCGCCT TCGCCAGATC CCGCGCTCAG TCGTCCAATT CTGCCGTGGA CGGCACCGCC GGACCCGGCT CCACCGGATC TCGCGAGGGC AGAGGAAGTC TTCTAACATG CGGTGACGTG GAGGAGAATC CCGGCCCTAT GACCGAGTAC AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGAAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTCCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGCCGCCT CCCCGCCTGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAAATA AGATCTGCTT TTTGCTTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGTAGTAG TTCATGTCAT CTTATTATTC AGTATTTATA ACTTGCAAAG AAATGAATAT CAGAGAGTGA GAGGAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGCTCT AGCTATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGACTTTTG CAGAGACGGC CCAAATTCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCAAG CTG
