pBV220-Pdcd1(mouse)
$70.00
In stock
SKU
PL43859
NM_008798.3;Ly101; PD-1; Pdc1
100μg liquid plasmid(Instantly):$91
| name | pBV220-Pdcd1(mouse) | another name | apBV220-Pdcd1(mouse) |
| Insert Size (bp) | 867bp | Plasmid host | Escherichia coli |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | PL,PR | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | pBV220-F | |
| 3′ sequencing primer | pBV220-R | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTGACTATTT TACCTCTGGC GGTGATAATG GTTGCATGTA CTAAGGAGGT TGTATGGAAC AACGCATAAC CCTGAAAGAT TATGCAATGC GCTTTGGGCA AACCAAGACA GCTAAAGATC TCTCACCTAC CAAACAATGC CCCCCTGCAA AAAATAAATT CATATAAAAA ACATACAGAT AACCATCTGC GGTGATAAAT TATCTCTGGC GGTGTTGACA TAAATACCAC TGGCGGTGAT ACTGAGCACA TCAGCAGGAC GCACTGACCA CCATGAAGGT GACGCTCTTA AAAATTAAGC CCTGAAGAAG GGCAGCATTC AAAGCAGAAG GCTTTGGGGT GTGTGATACG AAACGAAGCA TTGGTTAAAA ATTAAGGAGG GAATTCATGT GGGTCCGGCA GGTACCCTGG TCATTCACTT GGGCTGTGCT GCAGTTGAGC TGGCAATCAG GGTGGCTTCT AGAGGTCCCC AATGGGCCCT GGAGGTCCCT CACCTTCTAC CCAGCCTGGC TCACAGTGTC AGAGGGAGCA AATGCCACCT TCACCTGCAG CTTGTCCAAC TGGTCGGAGG ATCTTATGCT GAACTGGAAC CGCCTGAGTC CCAGCAACCA GACTGAAAAA CAGGCCGCCT TCTGTAATGG TTTGAGCCAA CCCGTCCAGG ATGCCCGCTT CCAGATCATA CAGCTGCCCA ACAGGCATGA CTTCCACATG AACATCCTTG ACACACGGCG CAATGACAGT GGCATCTACC TCTGTGGGGC CATCTCCCTG CACCCCAAGG CAAAAATCGA GGAGAGCCCT GGAGCAGAGC TCGTGGTAAC AGAGAGAATC CTGGAGACCT CAACAAGATA TCCCAGCCCC TCGCCCAAAC CAGAAGGCCG GTTTCAAGGC ATGGTCATTG GTATCATGAG TGCCCTAGTG GGTATCCCTG TATTGCTGCT GCTGGCCTGG GCCCTAGCTG TCTTCTGCTC AACAAGTATG TCAGAGGCCA GAGGAGCTGG AAGCAAGGAC GACACTCTGA AGGAGGAGCC TTCAGCAGCA CCTGTCCCTA GTGTGGCCTA TGAGGAGCTG GACTTCCAGG GACGAGAGAA GACACCAGAG CTCCCTACCG CCTGTGTGCA CACAGAATAT GCCACCATTG TCTTCACTGA AGGGCTGGGT GCCTCGGCCA TGGGACGTAG GGGCTCAGCT GATGGCCTGC AGGGTCCTCG GCCTCCAAGA CATGAGGATG GACATTGTTC TTGGCCTCTT TGAGTCGACC TGCAGCCAAG CTTGGCTGTT TTGGCGGATG AGAGAAGATT TTCAGCCTGA TACAGATTAA ATCAGAACGC AGAAGCGGTC TGATAAAACA GAATTTGCCT GGCGGCAGTA GCGCGGTGGT CCCACCTGAC CCCATGCCGA ACTCAGAAGT GAAACGCCGT AGCGCCGATG GTAGTGTGGG GTCTCCCCAT GCGAGAGTAG GGAACTGCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGGG AGCGGATTTG AACGTTGCGA AGCAACGGCC CGGAGGGTGG CGGGCAGGAC GCCCGCCATA AACTGCCAGG CATCAAATTA AGCAGAAGGC CATCCTGACG GATGGCCTTT TTGCGTTTCT ACAAACTCTT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCTTA TCTTTCCCTT TATTTTTGCT GCGGTAAGTC GCATAAAAAC CATTCTTCAT AATTCAATCC ATTTACTATG TTATGTTCTG AGGGGAGTGA AAATTCCCCT AATTCGATGA AGATTCTTGC TCAATTGTTA TCAGCTATGC GCCGACCAGA ACACCTTGCC GATCAGCCAA ACGTCTCTTC AGGCCACTGA CTAGCGATAA CTTTCCCCAC AACGGAACAA CTCTCATTGC ATGGGATCAT TGGGTACTGT GGGTTTAGTG GTTGTAAAAA CACCTGACCG CTATCCCTGA TCAGTTTCTT GAAGGTAAAC TCATCACCCC CAAGTCTGGC TATGCAGAAA TCACCTGGCT CAACAGCCTG CTCAGGGTCA ACGAGAATTA ACATTCCGTC AGGAAAGCTT GGCTTGGAGC CTGTTGGTGC GGTCATGGAA TTACCTTCAA CCTCAAGCCA GAATGCAGAA TCACTGGCTT TTTTGGTTGT GCTTACCCAT CTCTCCGCAT CACCTTTGGT AAAGGTTCTA AGCTTAGGTG AGAACATCCC TGCCTGAACA TGAGAAAAAA CAGGGTACTC ATACTCACTT CTAAGTGACG GCTGCATACT AACCGCTTCA TACATCTCGT AGATTTCTCT GGCGATTGAA GGGCTAAATT CTTCAACGCT AACTTTGAGA ATTTTTGTAA GCAATGCGGC GTTATAAGCA TTTAATGCAT TGATGCCATT AAATAAAGCA CCAACGCCTG ACTGCCCCAT CCCCATCTTG TCTGCGACAG ATTCCTGGGA TAAGCCAAGT TCATTTTTCT TTTTTTCATA AATTGCTTTA AGGCGACGTG CGTCCTCAAG CTGCTCTTGT GTTAATGGTT TCTTTTTTGT GCTCATACGT TAAATCTATC ACCGCAAGGG ATAAATATCT AACACCGTGC GTG
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