pGADT7-SLC39A14(human)
$84.00
In stock
SKU
PL43792
NM_001128431.4;cig19; HCIN; HMNDYT2; LZT-Hs4; NET34;ZIP14
100μg liquid plasmid(Instantly):$105
| name | pGADT7-SLC39A14(human) | another name | apGADT7-SLC39A14(human) |
| Insert Size (bp) | 1479bp | Plasmid host | Yeast bacterium |
| Plasmid uses | hybridization | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | LEU2 | Fluorescent labeling | |
| Promoter | ADH1 | Replicon | 2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | T7：TAATACGACTCACTATAGGG | |
| 3′ sequencing primer | ADH1-R：GAGTCACTTTAAAATTTGTATACAC | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGCATGCCTG CAGGTCGAGA TCCGGGATCG AAGAAATGAT GGTAAATGAA ATAGGAAATC AAGGAGCATG AAGGCAAAAG ACAAATATAA GGGTCGAACG AAAAATAAAG TGAAAAGTGT TGATATGATG TATTTGGCTT TGCGGCGCCG AAAAAACGAG TTTACGCAAT TGCACAATCA TGCTGACTCT GTGGCGGACC CGCGCTCTTG CCGGCCCGGC GATAACGCTG GGCGTGAGGC TGTGCCCGGC GGAGTTTTTT GCGCCTGCAT TTTCCAAGGT TTACCCTGCG CTAAGGGGCG AGATTGGAGA AGCAATAAGA ATGCCGGTTG GGGTTGCGAT GATGACGACC ACGACAACTG GTGTCATTAT TTAAGTTGCC GAAAGAACCT GAGTGCATTT GCAACATGAG TATACTAGAA GAATGAGCCA AGACTTGCGA GACGCGAGTT TGCCGGTGGT GCGAACAATA GAGCGACCAT GACCTTGAAG GTGAGACGCG CATAACCGCT AGAGTACTTT GAAGAGGAAA CAGCAATAGG GTTGCTACCA GTATAAATAG ACAGGTACAT ACAACACTGG AAATGGTTGT CTGTTTGAGT ACGCTTTCAA TTCATTTGGG TGTGCACTTT ATTATGTTAC AATATGGAAG GGAACTTTAC ACTTCTCCTA TGCACATATA TTAATTAAAG TCCAATGCTA GTAGAGAAGG GGGGTAACAC CCCTCCGCGC TCTTTTCCGA TTTTTTTCTA AACCGTGGAA TATTTCGGAT ATCCTTTTGT TGTTTCCGGG TGTACAATAT GGACTTCCTC TTTTCTGGCA ACCAAACCCA TACATCGGGA TTCCTATAAT ACCTTCGTTG GTCTCCCTAA CATGTAGGTG GCGGAGGGGA GATATACAAT AGAACAGATA CCAGACAAGA CATAATGGGC TAAACAAGAC TACACCAATT ACACTGCCTC ATTGATGGTG GTACATAACG AACTAATACT GTAGCCCTAG ACTTGATAGC CATCATCATA TCGAAGTTTC ACTACCCTTT TTCCATTTGC CATCTATTGA AGTAATAATA GGCGCATGCA ACTTCTTTTC TTTTTTTTTC TTTTCTCTCT CCCCCGTTGT TGTCTCACCA TATCCGCAAT GACAAAAAAA ATGATGGAAG ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA GATGTCGTTG TTCCAGAGCT GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC TTCATTCACG CACACTACTC TCTAATGAGC AACGGTATAC GGCCTTCCTT CCAGTTACTT GAATTTGAAA TAAAAAAAAG TTTGCTGTCT TGCTATCAAG TATAAATAGA CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTTGCAAA GATGGATAAA GCGGAATTAA TTCCCGAGCC TCCAAAAAAG AAGAGAAAGG TCGAATTGGG TACCGCCGCC AATTTTAATC AAAGTGGGAA TATTGCTGAT AGCTCATTGT CCTTCACTTT CACTAACAGT AGCAACGGTC CGAACCTCAT AACAACTCAA ACAAATTCTC AAGCGCTTTC ACAACCAATT GCCTCCTCTA ACGTTCATGA TAACTTCATG AATAATGAAA TCACGGCTAG TAAAATTGAT GATGGTAATA ATTCAAAACC ACTGTCACCT GGTTGGACGG ACCAAACTGC GTATAACGCG TTTGGAATCA CTACAGGGAT GTTTAATACC ACTACAATGG ATGATGTATA TAACTATCTA TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGA TCTTTAATAC GACTCACTAT AGGGCGAGCG CCGCCATGGA GTACCCATAC GACGTACCAG ATTACGCTCA TATGGCCATG GAGGCCAGTG AATTCATGAA GCTGCTGCTG CTGCACCCGG CCTTCCAGAG CTGCCTCCTG CTGACCCTGC TTGGCTTATG GAGAACCACC CCTGAGGCTC ACGCTTCATC CCTGGGTGCA CCAGCTATCA GCGCTGCCTC CTTCCTGCAG GATCTAATAC ATCGGTATGG CGAGGGTGAC AGCCTCACTC TGCAGCAGCT GAAGGCCCTA CTCAACCACC TGGATGTGGG AGTGGGCCGG GGTAATGTCA CCCAGCACGT GCAAGGACAC AGGAACCTCT CCACGTGCTT TAGTTCTGGA GACCTCTTCA CTGCCCACAA TTTCAGCGAG CAGTCGCGGA TTGGGAGCAG CGAGCTCCAG GAGTTCTGCC CCACCATCCT CCAGCAGCTG GATTCCCGGG CCTGCACCTC GGAGAACCAG GAAAACGAGG AGAATGAGCA GACGGAGGAG GGGCGGCCAA GCGCTGTTGA AGTGTGGGGA TACGGTCTCC TCTGTGTGAC CGTCATCTCC CTCTGCTCCC TCCTGGGGGC CAGCGTGGTG CCCTTCATGA AGAAGACCTT TTACAAGAGG CTGCTGCTCT ACTTCATAGC TCTGGCGATT GGAACCCTCT ACTCCAACGC CCTCTTCCAG CTCATCCCGG AGGCATTTGG TTTCAACCCT CTGGAAGATT ATTATGTCTC CAAGTCTGCA GTGGTGTTTG GGGGCTTTTA TCTTTTCTTT TTCACAGAGA AGATCTTGAA GATTCTTCTT AAGCAGAAAA ATGAGCATCA TCATGGACAC AGCCATTATG CCTCTGAGTC GCTTCCCTCC AAGAAGGACC AGGAGGAGGG GGTGATGGAG AAGCTGCAGA ACGGGGACCT GGACCACATG ATTCCTCAGC ACTGCAGCAG TGAGCTGGAC GGCAAGGCGC CCATGGTGGA CGAGAAGGTC ATTGTGGGCT CGCTCTCTGT GCAGGACCTG CAGGCTTCCC AGAGTGCTTG CTACTGGCTG AAAGGTGTCC GCTACTCTGA TATCGGCACT CTGGCCTGGA TGATCACTCT GAGCGACGGC CTCCATAATT TCATCGATGG CCTGGCCATC GGTGCTTCCT TCACTGTGTC AGTTTTCCAA GGCATCAGCA CCTCGGTGGC CATCCTCTGT GAGGAGTTCC CACATGAGCT AGGAGACTTT GTCATCCTGC TCAACGCTGG GATGAGCATC CAACAAGCTC TCTTCTTCAA CTTCCTTTCT GCCTGCTGCT GCTACCTGGG TCTGGCCTTT GGCATCCTGG CCGGCAGCCA CTTCTCTGCC AACTGGATTT TTGCGCTAGC TGGAGGAATG TTCTTGTATA TTTCTCTGGC TGATATGTTC CCTGAGATGA ATGAGGTCTG TCAAGAGGAT GAAAGGAAGG GCAGCATCTT GATTCCATTT ATCATCCAGA ACCTGGGCCT CCTGACTGGA TTCACCATCA TGGTGGTCCT CACCATGTAT TCAGGACAGA TCCAGATTGG GTAGGGATCC ATCGAGCTCG AGCTGCAGAT GAATCGTAGA TACTGAAAAA CCCCGCAAGT TCACTTCAAC TGTGCATCGT GCACCATCTC AATTTCTTTC ATTTATACAT CGTTTTGCCT TCTTTTATGT AACTATACTC CTCTAAGTTT CAATCTTGGC CATGTAACCT CTGATCTATA GAATTTTTTA AATGACTAGA ATTAATGCCC ATCTTTTTTT TGGACCTAAA TTCTTCATGA AAATATATTA CGAGGGCTTA TTCAGAAGCT TTGGACTTCT TCGCCAGAGG TTTGGTCAAG TCTCCAATCA AGGTTGTCGG CTTGTCTACC TTGCCAGAAA TTTACGAAAA GATGGAAAAG GGTCAAATCG TTGGTAGATA CGTTGTTGAC ACTTCTAAAT AAGCGAATTT CTTATGATTT ATGATTTTTA TTATTAAATA AGTTATAAAA AAAATAAGTG TATACAAATT TTAAAGTGAC TCTTAGGTTT TAAAACGAAA ATTCTTATTC TTGAGTAACT CTTTCCTGTA GGTCAGGTTG CTTTCTCAGG TATAGCATGA GGTCGCTCTT ATTGACCACA CCTCTACCGG CCGGTCGAAA TTCCCCTACC CTATGAACAT ATTCCATTTT GTAATTTCGT GTCGTTTCTA TTATGAATTT CATTTATAAA GTTTATGTAC AAATATCATA AAAAAAGAGA ATCTTTTTAA GCAAGGATTT TCTTAACTTC TTCGGCGACA GCATCACCGA CTTCGGTGGT ACTGTTGGAA CCACCTAAAT CACCAGTTCT GATACCTGCA TCCAAAACCT TTTTAACTGC ATCTTCAATG GCCTTACCTT CTTCAGGCAA GTTCAATGAC AATTTCAACA TCATTGCAGC AGACAAGATA GTGGCGATAG GGTTGACCTT ATTCTTTGGC AAATCTGGAG CAGAACCGTG GCATGGTTCG TACAAACCAA ATGCGGTGTT CTTGTCTGGC AAAGAGGCCA AGGACGCAGA TGGCAACAAA CCCAAGGAAC CTGGGATAAC GGAGGCTTCA TCGGAGATGA TATCACCAAA CATGTTGCTG GTGATTATAA TACCATTTAG GTGGGTTGGG TTCTTAACTA GGATCATGGC GGCAGAATCA ATCAATTGAT GTTGAACCTT CAATGTAGGA AATTCGTTCT TGATGGTTTC CTCCACAGTT TTTCTCCATA ATCTTGAAGA GGCCAAAACA TTAGCTTTAT CCAAGGACCA AATAGGCAAT GGTGGCTCAT GTTGTAGGGC CATGAAAGCG GCCATTCTTG TGATTCTTTG CACTTCTGGA ACGGTGTATT GTTCACTATC CCAAGCGACA CCATCACCAT CGTCTTCCTT TCTCTTACCA AAGTAAATAC CTCCCACTAA TTCTCTGACA ACAACGAAGT CAGTACCTTT AGCAAATTGT GGCTTGATTG GAGATAAGTC TAAAAGAGAG TCGGATGCAA AGTTACATGG TCTTAAGTTG GCGTACAATT GAAGTTCTTT ACGGATTTTT AGTAAACCTT GTTCAGGTCT AACACTACCT GTACCCCATT TAGGACCACC CACAGCACCT AACAAAACGG CATCAACCTT CTTGGAGGCT TCCAGCGCCT CATCTGGAAG TGGGACACCT GTAGCGTCGA TAGCAGCACC ACCAATTAAA TGATTTTCGA AATCGAACTT GACATTGGAA CGAACATCAG AAATAGCTTT AAGAACCTTA ATGGCTTCGG CTGTGATTTC TTGACCAACG TGGTCACCTG GCAAAACGAC GATCTTCTTA GGGGCAGACA TTAGAATGGT ATATCCTTGA AATATATATA TATATTGCTG AAATGTAAAA GGTAAGAAAA GTTAGAAAGT AAGACGATTG CTAACCACCT ATTGGAAAAA ACAATAGGTC CTTAAATAAT ATTGTCAACT TCAAGTATTG TGATGCAAGC ATTTAGTCAT GAACGCTTCT CTATTCTATA TGAAAAGCCG GTTCCGGCGC TCTCACCTTT CCTTTTTCTC CCAATTTTTC AGTTGAAAAA GGTATATGCG TCAGGCGACC TCTGAAATTA ACAAAAAATT TCCAGTCATC GAATTTGATT CTGTGCGATA GCGCCCCTGT GTGTTCTCGT TATGTTGAGG AAAAAAATAA TGGTTGCTAA GAGATTCGAA CTCTTGCATC TTACGATACC TGAGTATTCC CACAGTTGGG GATCTCGACT CTAGCTAGAG GATCAATTCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGATA ACTTCGTATA ATGTATGCTA TACGAAGTTA TTAGGTCTGA AGAGGAGTTT ACGTCCAGCC AAGCTAGCTT GGCTGCAGGT CGAGCGGCCG CGATCCGGAA CCCTTAATAT AACTTCGTAT AATGTATGCT ATACGAAGTT ATCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATAACG CATTTAAGCA TAAACACGCA CTATGCCGTT CTTCTCATGT ATATATATAT ACAGGCAACA CGCAGATATA GGTGCGACGT GAACAGTGAG CTGTATGTGC GCAGCTCGCG TTGCATTTTC GGAAGCGCTC GTTTTCGGAA ACGCTTTGAA GTTCCTATTC CGAAGTTCCT ATTCTCTAGC TAGAAAGTAT AGGAACTTCA GAGCGCTTTT GAAAACCAAA AGCGCTCTGA AGACGCACTT TCAAAAAACC AAAAACGCAC CGGACTGTAA CGAGCTACTA AAATATTGCG AATACCGCTT CCACAAACAT TGCTCAAAAG TATCTCTTTG CTATATATCT CTGTGCTATA TCCCTATATA ACCTACCCAT CCACCTTTCG CTCCTTGAAC TTGCATCTAA ACTCGACCTC TACATTTTTT ATGTTTATCT CTAGTATTAC TCTTTAGACA AAAAAATTGT AGTAAGAACT ATTCATAGAG TGAATCGAAA ACAATACGAA AATGTAAACA TTTCCTATAC GTAGTATATA GAGACAAAAT AGAAGAAACC GTTCATAATT TTCTGACCAA TGAAGAATCA TCAACGCTAT CACTTTCTGT TCACAAAGTA TGCGCAATCC ACATCGGTAT AGAATATAAT CGGGGATGCC TTTATCTTGA AAAAATGCAC CCGCAGCTTC GCTAGTAATC AGTAAACGCG GGAAGTGGAG TCAGGCTTTT TTTATGGAAG AGAAAATAGA CACCAAAGTA GCCTTCTTCT AACCTTAACG GACCTACAGT GCAAAAAGTT ATCAAGAGAC TGCATTATAG AGCGCACAAA GGAGAAAAAA AGTAATCTAA GATGCTTTGT TAGAAAAATA GCGCTCTCGG GATGCATTTT TGTAGAACAA AAAAGAAGTA TAGATTCTTT GTTGGTAAAA TAGCGCTCTC GCGTTGCATT TCTGTTCTGT AAAAATGCAG CTCAGATTCT TTGTTTGAAA AATTAGCGCT CTCGCGTTGC ATTTTTGTTT TACAAAAATG AAGCACAGAT TCTTCGTTGG TAAAATAGCG CTTTCGCGTT GCATTTCTGT TCTGTAAAAA TGCAGCTCAG ATTCTTTGTT TGAAAAATTA GCGCTCTCGC GTTGCATTTT TGTTCTACAA AATGAAGCAC AGATGCTTCG TTGCT
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