pTriEx-P10-MOG(human)-3×FLAG
$84.00
In stock
SKU
PL43768
NM_002433.5;BTN6; BTNL11; MOGIG2; NRCLP7
100μg liquid plasmid(Instantly):$105
| name | pTriEx-P10-MOG(human)-3×FLAG | another name | apTriEx-P10-MOG(human)-3×FLAG |
| Insert Size (bp) | 759bp | Plasmid host | Escherichia coli,Mammary cell,Insect cells |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | T7,P10 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCCTGCATCT TTTAATCAAA TCCCAAGATG TGTATAAACG CGCCGGTATG TACAGGAAGA GGTTTATACT AAACTGTTAC ATTGCAAACG TGGTTTCGTG TGCCAAGTGT GAAAACCGAT GTTTAATCAA GGCTCTGACG CATTTCTACA ACCACGACTC CAAGTGTGTG GGTGAAGTCA TGCATCTTTT AATCAAATCC CAAGATGTGT ATAAACCACC AAACTGCCAA AAAATGAAAA CTGTCGACAA GCTCTGTCCG TTTGCTGGCA ACTGCAAGGG TCTCAATCCT ATTTGTAATT ATTGAATAAT AAAACAATTA TAAATGTCAA ATTTGTTTTT TATTAACGAT ACAAACCAAA CGCAACAAGA ACATTTGTAG TATTATCTAT AATTGAAAAC GCGTAGTTAT AATCGCTGAG GTAATATTTA AAATCATTTT CAAATGATTC ACAGTTAATT TGCGACAATA TAATTTTATT TTCACATAAA CTAGACGCCT TGTCGTCTTC TTCTTCGTAT TCCTTCTCTT TTTCATTTTT CTCTTCATAA AAATTAACAT AGTTATTATC GTATCCATAT ATGTATCTAT CGTATAGAGT AAATTTTTTG TTGTCATAAA TATATATGTC TTTTTTAATG GGGTGTATAG TACCGCTGCG CATAGTTTTT CTGTAATTTA CAACAGTGCT ATTTTCTGGT AGTTCTTCGG AGTGTGTTGC TTTAATTATT AAATTTATAT AATCAATGAA TTTGGGATCG TCGGTTTTGT ACAATATGTT GCCGGCATAG TACGCAGCTT CTTCTAGTTC AATTACACCA TTTTTTAGCA GCACCGGATT AACATAACTT TCCAAAATGT TGTACGAACC GTTAAACAAA AACAGTTCAC CTCCCTTTTC TATACTATTG TCTGCGAGCA GTTGTTTGTT GTTAAAAATA ACAGCCATTG TAATGAGACG CACAAACTAA TATCACAAAC TGGAAATGTC TATCAATATA TAGTTGCTGA TGGCCGGCCG TAATGAGACG CACAAACTAA TATCACAAAC TGGAAATGTC TATCAATATA TAGTTGCTCT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGACT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGCATGGTC GAGGTGAGCC CCACGTTCTG CTTCACTCTC CCCATCTCCC CCCCCTCCCC ACCCCCAATT TTGTATTTAT TTATTTTTTA ATTATTTTGT GCAGCGATGG GGGCGGGGGG GGGGGGGGGG CGCGCGCCAG GCGGGGCGGG GCGGGGCGAG GGGCGGGGCG GGGCGAGGCG GAGAGGTGCG GCGGCAGCCA ATCAGAGCGG CGCGCTCCGA AAGTTTCCTT TTATGGCGAG GCGGCGGCGG CGGCGGCCCT ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGCG CTGCCTTCGC CCCGTGCCCC GCTCCGCCGC CGCCTCGCGC CGCCCGCCCC GGCTCTGACT GACCGCGTTA CTCCCACAGG TGAGCGGGCG GGACGGCCCT TCTCCTTCGG GCTGTAATTA GCGCTTGGTT TAATGACGGC TTGTTTCTTT TCTGTGGCTG CGTGAAAGCC TTGAGGGGCT CCGGGAGGGC CCTTTGTGCG GGGGGAGCGG CTCGGGGCTG TCCGCGGGGG GACGGCTGCC TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC GGCTCTAGAG CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTGGATCG GACCGAAATT AATACGACTC ACTATAGGGG AATTGTGAGC GGATAACAAT TCCCCGGAGT TAATCCGGGA CCTTTAATTC AACCCAACAC AATATATTAT AGTTAAATAA GAATTATTAT CAAATCATTT GTATATTAAT TAAAATACTA TACTGTAAAT TACATTTTAT TTACAATCAA AGGAGATATA CCATGGCGAT ATCCCGGGAG CTCGTGGATC CGAATTCGCC ACCATGGCAA GCTTATCAAG ACCCTCTCTG CCCAGCTGCC TCTGCTCCTT CCTCCTCCTC CTCCTCCTCC AAGTGTCTTC CAGCTATGCA GGGCAGTTCA GAGTGATAGG ACCAAGACAC CCTATCCGGG CTCTGGTCGG GGATGAAGTG GAATTGCCAT GTCGCATATC TCCTGGGAAG AACGCTACAG GCATGGAGGT GGGGTGGTAC CGCCCCCCCT TCTCTAGGGT GGTTCATCTC TACAGAAATG GCAAGGACCA AGATGGAGAC CAGGCACCTG AATATCGGGG CCGGACAGAG CTGCTGAAAG ATGCTATTGG TGAGGGAAAG GTGACTCTCA GGATCCGGAA TGTAAGGTTC TCAGATGAAG GAGGTTTCAC CTGCTTCTTC CGAGATCATT CTTACCAAGA GGAGGCAGCA ATGGAATTGA AAGTAGAAGA TCCTTTCTAC TGGGTGAGCC CTGGAGTGCT GGTTCTCCTC GCGGTGCTGC CTGTGCTCCT CCTGCAGATC ACTGTTGGCC TCATCTTCCT CTGCCTGCAG TACAGACTGA GAGGAAAACT TCGAGCAGAG ATAGAGAATC TCCACCGGAC TTTTGATCCC CACTTTCTGA GGGTGCCCTG CTGGAAGATA ACCCTGTTTG TAATTGTGCC GGTTCTTGGA CCCTTGGTTG CCTTGATCAT CTGCTACAAC TGGCTACATC GAAGACTAGC AGGGCAATTC CTTGAAGAGC TACTCTTCCA CCTGGAAGCC CTCTCTGGCG GTACCGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA GTGACTCGAG AGTGATTAAC CTCAGGTGCA GGCTGCCTAT CAGAAGGTGG TGGCTGGTGT GGCCAATGCC CTGGCTCACA AATACCACTG AGATCGATCT TTTTCCCTCT GCCAAAAATT ATGGGGACAT CATGAAGCCC CTTGAGCATC TGACTTCTGG CTAATAAAGG AAATTTATTT TCATTGCAAT AGTGTGTTGG AATTTTTTGT GTCTCTCACT CGGAAGGACA TATGGGAGGG CAAATCATTT AAAACATCAG AATGAGTATT TGGTTTAGAG TTTGGCAACA TATGCCCATA TGTAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG CATGCGGAGG AAATTCTCCT TGAAGTTTCC CTGGTGTTCA AAGTAAAGGA GTTTGCACCA GACGCACCTC TGTTCACTGG TCCGGCGTAT TAAAACACGA TACATTGTTA TTAGTACATT TATTAAGCGC TAGATTCTGT GCGTTGTTGA TTTACAGACA ATTGTTGTAC GTATTTTAAT AATTCATTAA ATTTATAATC TTTAGGGTGG TATGTTAGAG CGAAAATCAA ATGATTTTCA GCGTCTTTAT ATCTGAATTT AAATATTAAA TCCTCAATAG ATTTGTAAAA TAGGTTTCGA TTAGTTTCAA ACAAGGGTTG TTTTTCCGAA CCGATGGCTG GACTATCTAA TGGATTTTCG CTCAACGCCA CAAAACTTGC CAAATCTTGT AGCAGCAATC TAGCTTTGTC GATATTCGTT TGTGTTTTGT TTTGTAATAA AGGTTCGACG TCGTTCAAAA TATTATGCGC TTTTGTATTT CTTTCATCAC TGTCGTTAGT GTACAATTGA CTCGACGTAA ACACGTTAAA TAGAGCTTGG ACATATTTAA CATCGGGCGT GTTAGCTTTA TTAGGCCGAT TATCGTCGTC GTCCCAACCC TCGTCGTTAG AAGTTGCTTC CGAAGACGAT TTTGCCATAG CCACACGACG CCTATTAATT GTGTCGGCTA ACACGTCCGC GATCAAATTT GTAGTTGAGC TTTTTGGAAT TGCGATCGCA TAACTTCGTA TAGCATACAT TATACGAAGT TATAAGCTCG GAACGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGTCCGCGC GTT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
