NM_005765.3;APT6M8-9; ATP6IP2; ATP6M8-9; CDG2R; ELDF10;HT028; M8-9; MRXE; MRXSH; MSTP009; PRR; RENR; XMRE; XPDS
100μg liquid plasmid(Instantly):$105
| name | pGADT7-ATP6AP2(human) | another name | apGADT7-ATP6AP2(human) |
| Insert Size (bp) | 1053bp | Plasmid host | Yeast bacterium |
| Plasmid uses | hybridization | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | LEU2 | Fluorescent labeling | |
| Promoter | ADH1 | Replicon | 2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | T7：TAATACGACTCACTATAGGG | |
| 3′ sequencing primer | ADH1-R：GAGTCACTTTAAAATTTGTATACAC | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGCATGCCTG CAGGTCGAGA TCCGGGATCG AAGAAATGAT GGTAAATGAA ATAGGAAATC AAGGAGCATG AAGGCAAAAG ACAAATATAA GGGTCGAACG AAAAATAAAG TGAAAAGTGT TGATATGATG TATTTGGCTT TGCGGCGCCG AAAAAACGAG TTTACGCAAT TGCACAATCA TGCTGACTCT GTGGCGGACC CGCGCTCTTG CCGGCCCGGC GATAACGCTG GGCGTGAGGC TGTGCCCGGC GGAGTTTTTT GCGCCTGCAT TTTCCAAGGT TTACCCTGCG CTAAGGGGCG AGATTGGAGA AGCAATAAGA ATGCCGGTTG GGGTTGCGAT GATGACGACC ACGACAACTG GTGTCATTAT TTAAGTTGCC GAAAGAACCT GAGTGCATTT GCAACATGAG TATACTAGAA GAATGAGCCA AGACTTGCGA GACGCGAGTT TGCCGGTGGT GCGAACAATA GAGCGACCAT GACCTTGAAG GTGAGACGCG CATAACCGCT AGAGTACTTT GAAGAGGAAA CAGCAATAGG GTTGCTACCA GTATAAATAG ACAGGTACAT ACAACACTGG AAATGGTTGT CTGTTTGAGT ACGCTTTCAA TTCATTTGGG TGTGCACTTT ATTATGTTAC AATATGGAAG GGAACTTTAC ACTTCTCCTA TGCACATATA TTAATTAAAG TCCAATGCTA GTAGAGAAGG GGGGTAACAC CCCTCCGCGC TCTTTTCCGA TTTTTTTCTA AACCGTGGAA TATTTCGGAT ATCCTTTTGT TGTTTCCGGG TGTACAATAT GGACTTCCTC TTTTCTGGCA ACCAAACCCA TACATCGGGA TTCCTATAAT ACCTTCGTTG GTCTCCCTAA CATGTAGGTG GCGGAGGGGA GATATACAAT AGAACAGATA CCAGACAAGA CATAATGGGC TAAACAAGAC TACACCAATT ACACTGCCTC ATTGATGGTG GTACATAACG AACTAATACT GTAGCCCTAG ACTTGATAGC CATCATCATA TCGAAGTTTC ACTACCCTTT TTCCATTTGC CATCTATTGA AGTAATAATA GGCGCATGCA ACTTCTTTTC TTTTTTTTTC TTTTCTCTCT CCCCCGTTGT TGTCTCACCA TATCCGCAAT GACAAAAAAA ATGATGGAAG ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA GATGTCGTTG TTCCAGAGCT GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC TTCATTCACG CACACTACTC TCTAATGAGC AACGGTATAC GGCCTTCCTT CCAGTTACTT GAATTTGAAA TAAAAAAAAG TTTGCTGTCT TGCTATCAAG TATAAATAGA CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTTGCAAA GATGGATAAA GCGGAATTAA TTCCCGAGCC TCCAAAAAAG AAGAGAAAGG TCGAATTGGG TACCGCCGCC AATTTTAATC AAAGTGGGAA TATTGCTGAT AGCTCATTGT CCTTCACTTT CACTAACAGT AGCAACGGTC CGAACCTCAT AACAACTCAA ACAAATTCTC AAGCGCTTTC ACAACCAATT GCCTCCTCTA ACGTTCATGA TAACTTCATG AATAATGAAA TCACGGCTAG TAAAATTGAT GATGGTAATA ATTCAAAACC ACTGTCACCT GGTTGGACGG ACCAAACTGC GTATAACGCG TTTGGAATCA CTACAGGGAT GTTTAATACC ACTACAATGG ATGATGTATA TAACTATCTA TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGA TCTTTAATAC GACTCACTAT AGGGCGAGCG CCGCCATGGA GTACCCATAC GACGTACCAG ATTACGCTCA TATGGCCATG GAGGCCAGTG AATTCATGGC TGTGTTTGTC GTGCTCCTGG CGTTGGTGGC GGGTGTTTTG GGGAACGAGT TTAGTATATT AAAATCACCA GGGTCTGTTG TTTTCCGAAA TGGAAATTGG CCTATACCAG GAGAGCGGAT CCCAGACGTG GCTGCATTGT CCATGGGCTT CTCTGTGAAA GAAGACCTTT CTTGGCCAGG ACTCGCAGTG GGTAACCTGT TTCATCGTCC TCGGGCTACC GTCATGGTGA TGGTGAAGGG AGTGAACAAA CTGGCTCTAC CCCCAGGCAG TGTCATTTCG TACCCTTTGG AGAATGCAGT TCCTTTTAGT CTTGACAGTG TTGCAAATTC CATTCACTCC TTATTTTCTG AGGAAACTCC TGTTGTTTTG CAGTTGGCTC CCAGTGAGGA AAGAGTGTAT ATGGTAGGGA AGGCAAACTC AGTGTTTGAA GACCTTTCAG TCACCTTGCG CCAGCTCCGT AATCGCCTGT TTCAAGAAAA CTCTGTTCTC AGTTCACTCC CCCTCAATTC TCTGAGTAGG AACAATGAAG TTGACCTGCT CTTTCTTTCT GAACTGCAAG TGCTACATGA TATTTCAAGC TTGCTGTCTC GTCATAAGCA TCTAGCCAAG GATCATTCTC CTGATTTATA TTCACTGGAG CTGGCAGGTT TGGATGAAAT TGGGAAGCGT TATGGGGAAG ACTCTGAACA ATTCAGAGAT GCTTCTAAGA TCCTTGTTGA CGCTCTGCAA AAGTTTGCAG ATGACATGTA CAGTCTTTAT GGTGGGAATG CAGTGGTAGA GTTAGTCACT GTCAAGTCAT TTGACACCTC CCTCATTAGG AAGACAAGGA CTATCCTTGA GGCAAAACAA GCGAAGAACC CAGCAAGTCC CTATAACCTT GCATATAAGT ATAATTTTGA ATATTCCGTG GTTTTCAACA TGGTACTTTG GATAATGATC GCCTTGGCCT TGGCTGTGAT TATCACCTCT TACAATATTT GGAACATGGA TCCTGGATAT GATAGCATCA TTTATAGGAT GACAAACCAG AAGATTCGAA TGGATTGAGG ATCCATCGAG CTCGAGCTGC AGATGAATCG TAGATACTGA AAAACCCCGC AAGTTCACTT CAACTGTGCA TCGTGCACCA TCTCAATTTC TTTCATTTAT ACATCGTTTT GCCTTCTTTT ATGTAACTAT ACTCCTCTAA GTTTCAATCT TGGCCATGTA ACCTCTGATC TATAGAATTT TTTAAATGAC TAGAATTAAT GCCCATCTTT TTTTTGGACC TAAATTCTTC ATGAAAATAT ATTACGAGGG CTTATTCAGA AGCTTTGGAC TTCTTCGCCA GAGGTTTGGT CAAGTCTCCA ATCAAGGTTG TCGGCTTGTC TACCTTGCCA GAAATTTACG AAAAGATGGA AAAGGGTCAA ATCGTTGGTA GATACGTTGT TGACACTTCT AAATAAGCGA ATTTCTTATG ATTTATGATT TTTATTATTA AATAAGTTAT AAAAAAAATA AGTGTATACA AATTTTAAAG TGACTCTTAG GTTTTAAAAC GAAAATTCTT ATTCTTGAGT AACTCTTTCC TGTAGGTCAG GTTGCTTTCT CAGGTATAGC ATGAGGTCGC TCTTATTGAC CACACCTCTA CCGGCCGGTC GAAATTCCCC TACCCTATGA ACATATTCCA TTTTGTAATT TCGTGTCGTT TCTATTATGA ATTTCATTTA TAAAGTTTAT GTACAAATAT CATAAAAAAA GAGAATCTTT TTAAGCAAGG ATTTTCTTAA CTTCTTCGGC GACAGCATCA CCGACTTCGG TGGTACTGTT GGAACCACCT AAATCACCAG TTCTGATACC TGCATCCAAA ACCTTTTTAA CTGCATCTTC AATGGCCTTA CCTTCTTCAG GCAAGTTCAA TGACAATTTC AACATCATTG CAGCAGACAA GATAGTGGCG ATAGGGTTGA CCTTATTCTT TGGCAAATCT GGAGCAGAAC CGTGGCATGG TTCGTACAAA CCAAATGCGG TGTTCTTGTC TGGCAAAGAG GCCAAGGACG CAGATGGCAA CAAACCCAAG GAACCTGGGA TAACGGAGGC TTCATCGGAG ATGATATCAC CAAACATGTT GCTGGTGATT ATAATACCAT TTAGGTGGGT TGGGTTCTTA ACTAGGATCA TGGCGGCAGA ATCAATCAAT TGATGTTGAA CCTTCAATGT AGGAAATTCG TTCTTGATGG TTTCCTCCAC AGTTTTTCTC CATAATCTTG AAGAGGCCAA AACATTAGCT TTATCCAAGG ACCAAATAGG CAATGGTGGC TCATGTTGTA GGGCCATGAA AGCGGCCATT CTTGTGATTC TTTGCACTTC TGGAACGGTG TATTGTTCAC TATCCCAAGC GACACCATCA CCATCGTCTT CCTTTCTCTT ACCAAAGTAA ATACCTCCCA CTAATTCTCT GACAACAACG AAGTCAGTAC CTTTAGCAAA TTGTGGCTTG ATTGGAGATA AGTCTAAAAG AGAGTCGGAT GCAAAGTTAC ATGGTCTTAA GTTGGCGTAC AATTGAAGTT CTTTACGGAT TTTTAGTAAA CCTTGTTCAG GTCTAACACT ACCTGTACCC CATTTAGGAC CACCCACAGC ACCTAACAAA ACGGCATCAA CCTTCTTGGA GGCTTCCAGC GCCTCATCTG GAAGTGGGAC ACCTGTAGCG TCGATAGCAG CACCACCAAT TAAATGATTT TCGAAATCGA ACTTGACATT GGAACGAACA TCAGAAATAG CTTTAAGAAC CTTAATGGCT TCGGCTGTGA TTTCTTGACC AACGTGGTCA CCTGGCAAAA CGACGATCTT CTTAGGGGCA GACATTAGAA TGGTATATCC TTGAAATATA TATATATATT GCTGAAATGT AAAAGGTAAG AAAAGTTAGA AAGTAAGACG ATTGCTAACC ACCTATTGGA AAAAACAATA GGTCCTTAAA TAATATTGTC AACTTCAAGT ATTGTGATGC AAGCATTTAG TCATGAACGC TTCTCTATTC TATATGAAAA GCCGGTTCCG GCGCTCTCAC CTTTCCTTTT TCTCCCAATT TTTCAGTTGA AAAAGGTATA TGCGTCAGGC GACCTCTGAA ATTAACAAAA AATTTCCAGT CATCGAATTT GATTCTGTGC GATAGCGCCC CTGTGTGTTC TCGTTATGTT GAGGAAAAAA ATAATGGTTG CTAAGAGATT CGAACTCTTG CATCTTACGA TACCTGAGTA TTCCCACAGT TGGGGATCTC GACTCTAGCT AGAGGATCAA TTCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GATAACTTCG TATAATGTAT GCTATACGAA GTTATTAGGT CTGAAGAGGA GTTTACGTCC AGCCAAGCTA GCTTGGCTGC AGGTCGAGCG GCCGCGATCC GGAACCCTTA ATATAACTTC GTATAATGTA TGCTATACGA AGTTATCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT AACGCATTTA AGCATAAACA CGCACTATGC CGTTCTTCTC ATGTATATAT ATATACAGGC AACACGCAGA TATAGGTGCG ACGTGAACAG TGAGCTGTAT GTGCGCAGCT CGCGTTGCAT TTTCGGAAGC GCTCGTTTTC GGAAACGCTT TGAAGTTCCT ATTCCGAAGT TCCTATTCTC TAGCTAGAAA GTATAGGAAC TTCAGAGCGC TTTTGAAAAC CAAAAGCGCT CTGAAGACGC ACTTTCAAAA AACCAAAAAC GCACCGGACT GTAACGAGCT ACTAAAATAT TGCGAATACC GCTTCCACAA ACATTGCTCA AAAGTATCTC TTTGCTATAT ATCTCTGTGC TATATCCCTA TATAACCTAC CCATCCACCT TTCGCTCCTT GAACTTGCAT CTAAACTCGA CCTCTACATT TTTTATGTTT ATCTCTAGTA TTACTCTTTA GACAAAAAAA TTGTAGTAAG AACTATTCAT AGAGTGAATC GAAAACAATA CGAAAATGTA AACATTTCCT ATACGTAGTA TATAGAGACA AAATAGAAGA AACCGTTCAT AATTTTCTGA CCAATGAAGA ATCATCAACG CTATCACTTT CTGTTCACAA AGTATGCGCA ATCCACATCG GTATAGAATA TAATCGGGGA TGCCTTTATC TTGAAAAAAT GCACCCGCAG CTTCGCTAGT AATCAGTAAA CGCGGGAAGT GGAGTCAGGC TTTTTTTATG GAAGAGAAAA TAGACACCAA AGTAGCCTTC TTCTAACCTT AACGGACCTA CAGTGCAAAA AGTTATCAAG AGACTGCATT ATAGAGCGCA CAAAGGAGAA AAAAAGTAAT CTAAGATGCT TTGTTAGAAA AATAGCGCTC TCGGGATGCA TTTTTGTAGA ACAAAAAAGA AGTATAGATT CTTTGTTGGT AAAATAGCGC TCTCGCGTTG CATTTCTGTT CTGTAAAAAT GCAGCTCAGA TTCTTTGTTT GAAAAATTAG CGCTCTCGCG TTGCATTTTT GTTTTACAAA AATGAAGCAC AGATTCTTCG TTGGTAAAAT AGCGCTTTCG CGTTGCATTT CTGTTCTGTA AAAATGCAGC TCAGATTCTT TGTTTGAAAA ATTAGCGCTC TCGCGTTGCA TTTTTGTTCT ACAAAATGAA GCACAGATGC TTCGTTGCT
