pGADT7-TARDBP(human)
$84.00
In stock
SKU
PL43728
NM_007375.4;ALS10; TDP-43
100μg liquid plasmid(Instantly):$105
| name | pGADT7-TARDBP(human) | another name | apGADT7-TARDBP(human) |
| Insert Size (bp) | 1245bp | Plasmid host | Yeast bacterium |
| Plasmid uses | hybridization | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | LEU2 | Fluorescent labeling | |
| Promoter | ADH1 | Replicon | 2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | T7：TAATACGACTCACTATAGGG | |
| 3′ sequencing primer | ADH1-R：GAGTCACTTTAAAATTTGTATACAC | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGCATGCCTG CAGGTCGAGA TCCGGGATCG AAGAAATGAT GGTAAATGAA ATAGGAAATC AAGGAGCATG AAGGCAAAAG ACAAATATAA GGGTCGAACG AAAAATAAAG TGAAAAGTGT TGATATGATG TATTTGGCTT TGCGGCGCCG AAAAAACGAG TTTACGCAAT TGCACAATCA TGCTGACTCT GTGGCGGACC CGCGCTCTTG CCGGCCCGGC GATAACGCTG GGCGTGAGGC TGTGCCCGGC GGAGTTTTTT GCGCCTGCAT TTTCCAAGGT TTACCCTGCG CTAAGGGGCG AGATTGGAGA AGCAATAAGA ATGCCGGTTG GGGTTGCGAT GATGACGACC ACGACAACTG GTGTCATTAT TTAAGTTGCC GAAAGAACCT GAGTGCATTT GCAACATGAG TATACTAGAA GAATGAGCCA AGACTTGCGA GACGCGAGTT TGCCGGTGGT GCGAACAATA GAGCGACCAT GACCTTGAAG GTGAGACGCG CATAACCGCT AGAGTACTTT GAAGAGGAAA CAGCAATAGG GTTGCTACCA GTATAAATAG ACAGGTACAT ACAACACTGG AAATGGTTGT CTGTTTGAGT ACGCTTTCAA TTCATTTGGG TGTGCACTTT ATTATGTTAC AATATGGAAG GGAACTTTAC ACTTCTCCTA TGCACATATA TTAATTAAAG TCCAATGCTA GTAGAGAAGG GGGGTAACAC CCCTCCGCGC TCTTTTCCGA TTTTTTTCTA AACCGTGGAA TATTTCGGAT ATCCTTTTGT TGTTTCCGGG TGTACAATAT GGACTTCCTC TTTTCTGGCA ACCAAACCCA TACATCGGGA TTCCTATAAT ACCTTCGTTG GTCTCCCTAA CATGTAGGTG GCGGAGGGGA GATATACAAT AGAACAGATA CCAGACAAGA CATAATGGGC TAAACAAGAC TACACCAATT ACACTGCCTC ATTGATGGTG GTACATAACG AACTAATACT GTAGCCCTAG ACTTGATAGC CATCATCATA TCGAAGTTTC ACTACCCTTT TTCCATTTGC CATCTATTGA AGTAATAATA GGCGCATGCA ACTTCTTTTC TTTTTTTTTC TTTTCTCTCT CCCCCGTTGT TGTCTCACCA TATCCGCAAT GACAAAAAAA ATGATGGAAG ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA GATGTCGTTG TTCCAGAGCT GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC TTCATTCACG CACACTACTC TCTAATGAGC AACGGTATAC GGCCTTCCTT CCAGTTACTT GAATTTGAAA TAAAAAAAAG TTTGCTGTCT TGCTATCAAG TATAAATAGA CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTTGCAAA GATGGATAAA GCGGAATTAA TTCCCGAGCC TCCAAAAAAG AAGAGAAAGG TCGAATTGGG TACCGCCGCC AATTTTAATC AAAGTGGGAA TATTGCTGAT AGCTCATTGT CCTTCACTTT CACTAACAGT AGCAACGGTC CGAACCTCAT AACAACTCAA ACAAATTCTC AAGCGCTTTC ACAACCAATT GCCTCCTCTA ACGTTCATGA TAACTTCATG AATAATGAAA TCACGGCTAG TAAAATTGAT GATGGTAATA ATTCAAAACC ACTGTCACCT GGTTGGACGG ACCAAACTGC GTATAACGCG TTTGGAATCA CTACAGGGAT GTTTAATACC ACTACAATGG ATGATGTATA TAACTATCTA TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGA TCTTTAATAC GACTCACTAT AGGGCGAGCG CCGCCATGGA GTACCCATAC GACGTACCAG ATTACGCTCA TATGGCCATG GAGGCCAGTG AATTCATGTC TGAATATATT CGGGTAACCG AAGATGAGAA CGATGAGCCC ATTGAAATAC CATCGGAAGA CGATGGGACG GTGCTGCTCT CCACGGTTAC AGCCCAGTTT CCAGGGGCGT GTGGGCTTCG CTACAGGAAT CCAGTGTCTC AGTGTATGAG AGGTGTCCGG CTGGTAGAAG GAATTCTGCA TGCCCCAGAT GCTGGCTGGG GAAATCTGGT GTATGTTGTC AACTATCCAA AAGATAACAA AAGAAAAATG GATGAGACAG ATGCTTCATC AGCAGTGAAA GTGAAAAGAG CAGTCCAGAA AACATCCGAT TTAATAGTGT TGGGTCTCCC ATGGAAAACA ACCGAACAGG ACCTGAAAGA GTATTTTAGT ACCTTTGGAG AAGTTCTTAT GGTGCAGGTC AAGAAAGATC TTAAGACTGG TCATTCAAAG GGGTTTGGCT TTGTTCGTTT TACGGAATAT GAAACACAAG TGAAAGTAAT GTCACAGCGA CATATGATAG ATGGACGATG GTGTGACTGC AAACTTCCTA ATTCTAAGCA AAGCCAAGAT GAGCCTTTGA GAAGCAGAAA AGTGTTTGTG GGGCGCTGTA CAGAGGACAT GACTGAGGAT GAGCTGCGGG AGTTCTTCTC TCAGTACGGG GATGTGATGG ATGTCTTCAT CCCCAAGCCA TTCAGGGCCT TTGCCTTTGT TACATTTGCA GATGATCAGA TTGCGCAGTC TCTTTGTGGA GAGGACTTGA TCATTAAAGG AATCAGCGTT CATATATCCA ATGCCGAACC TAAGCACAAT AGCAATAGAC AGTTAGAAAG AAGTGGAAGA TTTGGTGGTA ATCCAGGTGG CTTTGGGAAT CAGGGTGGAT TTGGTAATAG CAGAGGGGGT GGAGCTGGTT TGGGAAACAA TCAAGGTAGT AATATGGGTG GTGGGATGAA CTTTGGTGCG TTCAGCATTA ATCCAGCCAT GATGGCTGCC GCCCAGGCAG CACTACAGAG CAGTTGGGGT ATGATGGGCA TGTTAGCCAG CCAGCAGAAC CAGTCAGGCC CATCGGGTAA TAACCAAAAC CAAGGCAACA TGCAGAGGGA GCCAAACCAG GCCTTCGGTT CTGGAAATAA CTCTTATAGT GGCTCTAATT CTGGTGCAGC AATTGGTTGG GGATCAGCAT CCAATGCAGG GTCGGGCAGT GGTTTTAATG GAGGCTTTGG CTCAAGCATG GATTCTAAGT CTTCTGGCTG GGGAATGTAG GGATCCATCG AGCTCGAGCT GCAGATGAAT CGTAGATACT GAAAAACCCC GCAAGTTCAC TTCAACTGTG CATCGTGCAC CATCTCAATT TCTTTCATTT ATACATCGTT TTGCCTTCTT TTATGTAACT ATACTCCTCT AAGTTTCAAT CTTGGCCATG TAACCTCTGA TCTATAGAAT TTTTTAAATG ACTAGAATTA ATGCCCATCT TTTTTTTGGA CCTAAATTCT TCATGAAAAT ATATTACGAG GGCTTATTCA GAAGCTTTGG ACTTCTTCGC CAGAGGTTTG GTCAAGTCTC CAATCAAGGT TGTCGGCTTG TCTACCTTGC CAGAAATTTA CGAAAAGATG GAAAAGGGTC AAATCGTTGG TAGATACGTT GTTGACACTT CTAAATAAGC GAATTTCTTA TGATTTATGA TTTTTATTAT TAAATAAGTT ATAAAAAAAA TAAGTGTATA CAAATTTTAA AGTGACTCTT AGGTTTTAAA ACGAAAATTC TTATTCTTGA GTAACTCTTT CCTGTAGGTC AGGTTGCTTT CTCAGGTATA GCATGAGGTC GCTCTTATTG ACCACACCTC TACCGGCCGG TCGAAATTCC CCTACCCTAT GAACATATTC CATTTTGTAA TTTCGTGTCG TTTCTATTAT GAATTTCATT TATAAAGTTT ATGTACAAAT ATCATAAAAA AAGAGAATCT TTTTAAGCAA GGATTTTCTT AACTTCTTCG GCGACAGCAT CACCGACTTC GGTGGTACTG TTGGAACCAC CTAAATCACC AGTTCTGATA CCTGCATCCA AAACCTTTTT AACTGCATCT TCAATGGCCT TACCTTCTTC AGGCAAGTTC AATGACAATT TCAACATCAT TGCAGCAGAC AAGATAGTGG CGATAGGGTT GACCTTATTC TTTGGCAAAT CTGGAGCAGA ACCGTGGCAT GGTTCGTACA AACCAAATGC GGTGTTCTTG TCTGGCAAAG AGGCCAAGGA CGCAGATGGC AACAAACCCA AGGAACCTGG GATAACGGAG GCTTCATCGG AGATGATATC ACCAAACATG TTGCTGGTGA TTATAATACC ATTTAGGTGG GTTGGGTTCT TAACTAGGAT CATGGCGGCA GAATCAATCA ATTGATGTTG AACCTTCAAT GTAGGAAATT CGTTCTTGAT GGTTTCCTCC ACAGTTTTTC TCCATAATCT TGAAGAGGCC AAAACATTAG CTTTATCCAA GGACCAAATA GGCAATGGTG GCTCATGTTG TAGGGCCATG AAAGCGGCCA TTCTTGTGAT TCTTTGCACT TCTGGAACGG TGTATTGTTC ACTATCCCAA GCGACACCAT CACCATCGTC TTCCTTTCTC TTACCAAAGT AAATACCTCC CACTAATTCT CTGACAACAA CGAAGTCAGT ACCTTTAGCA AATTGTGGCT TGATTGGAGA TAAGTCTAAA AGAGAGTCGG ATGCAAAGTT ACATGGTCTT AAGTTGGCGT ACAATTGAAG TTCTTTACGG ATTTTTAGTA AACCTTGTTC AGGTCTAACA CTACCTGTAC CCCATTTAGG ACCACCCACA GCACCTAACA AAACGGCATC AACCTTCTTG GAGGCTTCCA GCGCCTCATC TGGAAGTGGG ACACCTGTAG CGTCGATAGC AGCACCACCA ATTAAATGAT TTTCGAAATC GAACTTGACA TTGGAACGAA CATCAGAAAT AGCTTTAAGA ACCTTAATGG CTTCGGCTGT GATTTCTTGA CCAACGTGGT CACCTGGCAA AACGACGATC TTCTTAGGGG CAGACATTAG AATGGTATAT CCTTGAAATA TATATATATA TTGCTGAAAT GTAAAAGGTA AGAAAAGTTA GAAAGTAAGA CGATTGCTAA CCACCTATTG GAAAAAACAA TAGGTCCTTA AATAATATTG TCAACTTCAA GTATTGTGAT GCAAGCATTT AGTCATGAAC GCTTCTCTAT TCTATATGAA AAGCCGGTTC CGGCGCTCTC ACCTTTCCTT TTTCTCCCAA TTTTTCAGTT GAAAAAGGTA TATGCGTCAG GCGACCTCTG AAATTAACAA AAAATTTCCA GTCATCGAAT TTGATTCTGT GCGATAGCGC CCCTGTGTGT TCTCGTTATG TTGAGGAAAA AAATAATGGT TGCTAAGAGA TTCGAACTCT TGCATCTTAC GATACCTGAG TATTCCCACA GTTGGGGATC TCGACTCTAG CTAGAGGATC AATTCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGATAACTT CGTATAATGT ATGCTATACG AAGTTATTAG GTCTGAAGAG GAGTTTACGT CCAGCCAAGC TAGCTTGGCT GCAGGTCGAG CGGCCGCGAT CCGGAACCCT TAATATAACT TCGTATAATG TATGCTATAC GAAGTTATCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATAACGCATT TAAGCATAAA CACGCACTAT GCCGTTCTTC TCATGTATAT ATATATACAG GCAACACGCA GATATAGGTG CGACGTGAAC AGTGAGCTGT ATGTGCGCAG CTCGCGTTGC ATTTTCGGAA GCGCTCGTTT TCGGAAACGC TTTGAAGTTC CTATTCCGAA GTTCCTATTC TCTAGCTAGA AAGTATAGGA ACTTCAGAGC GCTTTTGAAA ACCAAAAGCG CTCTGAAGAC GCACTTTCAA AAAACCAAAA ACGCACCGGA CTGTAACGAG CTACTAAAAT ATTGCGAATA CCGCTTCCAC AAACATTGCT CAAAAGTATC TCTTTGCTAT ATATCTCTGT GCTATATCCC TATATAACCT ACCCATCCAC CTTTCGCTCC TTGAACTTGC ATCTAAACTC GACCTCTACA TTTTTTATGT TTATCTCTAG TATTACTCTT TAGACAAAAA AATTGTAGTA AGAACTATTC ATAGAGTGAA TCGAAAACAA TACGAAAATG TAAACATTTC CTATACGTAG TATATAGAGA CAAAATAGAA GAAACCGTTC ATAATTTTCT GACCAATGAA GAATCATCAA CGCTATCACT TTCTGTTCAC AAAGTATGCG CAATCCACAT CGGTATAGAA TATAATCGGG GATGCCTTTA TCTTGAAAAA ATGCACCCGC AGCTTCGCTA GTAATCAGTA AACGCGGGAA GTGGAGTCAG GCTTTTTTTA TGGAAGAGAA AATAGACACC AAAGTAGCCT TCTTCTAACC TTAACGGACC TACAGTGCAA AAAGTTATCA AGAGACTGCA TTATAGAGCG CACAAAGGAG AAAAAAAGTA ATCTAAGATG CTTTGTTAGA AAAATAGCGC TCTCGGGATG CATTTTTGTA GAACAAAAAA GAAGTATAGA TTCTTTGTTG GTAAAATAGC GCTCTCGCGT TGCATTTCTG TTCTGTAAAA ATGCAGCTCA GATTCTTTGT TTGAAAAATT AGCGCTCTCG CGTTGCATTT TTGTTTTACA AAAATGAAGC ACAGATTCTT CGTTGGTAAA ATAGCGCTTT CGCGTTGCAT TTCTGTTCTG TAAAAATGCA GCTCAGATTC TTTGTTTGAA AAATTAGCGC TCTCGCGTTG CATTTTTGTT CTACAAAATG AAGCACAGAT GCTTCGTTGC T
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