pRetroX-TetOne-Puro
$113.00
In stock
SKU
PL43685
100μg liquid plasmid(Instantly):$134
| name | pRetroX-TetOne-Puro | another name | apRetroX-TetOne-Puro |
| Insert Size (bp) | Plasmid host | Mammary cell,retrovirus | |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | TRE | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTAGC TTTGCTCTTA GGAGTTTCCT AATACATCCC AAACTCAAAT ATATAAAGCA TTTGACTTGT TCTATGCGCC TAGGAGCTTG GCCCATTGCA TACGTTGTAT CCATATCATA ATATGTACAT TTATATTGGC TCATGTCCAA CATTACCGCC ATGTTGACAT TGATTATTGA CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC ATCTAGAGCG CTTCTGCTCC CCGAGCTCTA TAAAAGAGCC CACAACCCCT CACTCGGCGC GCCAGTCCTC CGATTGACTG AGTCGCCCGG GTACCCGTGT ATCCAATAAA CCCTCTTGCA GTTGCATCCG ACTTGTGGTC TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG TGATTGACTA CCCGTCAGCG GGGGTCTTTC ATTTGGGGGC TCGTCCGGGA TCGGGAGACC CCTGCCCAGG GACCACCGAC CCACCACCGG GAGGTAAGCT GGCCAGCAAC TTATCTGTGT CTGTCCGATT GTCTAGTGTC TATGACTGAT TTTATGCGCC TGCGTCGGTA CTAGTTAGCT AACTAGCTCT GTATCTGGCG GACCCGTGGT GGAACTGACG AGTTCGGAAC ACCCGGCCGC AACCCTGGGA GACGTCCCAG GGACTTCGGG GGCCGTTTTT GTGGCCCGAC CTGAGTCCTA AAATCCCGAT CGTTTAGGAC TCTTTGGTGC ACCCCCCTTA GAGGAGGGAT ATGTGGTTCT GGTAGGAGAC GAGAACCTAA AACAGTTCCC GCCTCCGTCT GAATTTTTGC TTTCGGTTTG GGACCGAAGC CGCGCCGCGC GTCTTGTCTG CTGCAGCATC GTTCTGTGTT GTCTCTGTCT GACTGTGTTT CTGTATTTGT CTGAAAATGA GGGCCCGGGC TAGCCTGTTA CCACTCCCTT AAGTTTGACC TTAGGTCACT GGAAAGATGT CGAGCGGATC GCTCACAACC AGTCGGTAGA TGTCAAGAAG AGACGTTGGG TTACCTTCTG CTCTGCAGAA TGGCCAACCT TTAACGTCGG ATGGCCGCGA GACGGCACCT TTAACCGAGA CCTCATCACC CAGGTTAAGA TCAAGGTCTT TTCACCTGGC CCGCATGGAC ACCCAGACCA GGTGGGGTAC ATCGTGACCT GGGAAGCCTT GGCTTTTGAC CCCCCTCCCT GGGTCAAGCC CTTTGTACAC CCTAAGCCTC CGCCTCCTCT TCCTCCATCC GCCCCGTCTC TCCCCCTTGA ACCTCCTCGT TCGACCCCGC CTCGATCCTC CCTTTATCCA GCCCTCACTC CTTCTCTAGG CGCCCCCATA TGGCCATATG AGATCCTATA TGGGGCACCC CCGCCCCTTG TAAACTTCCC TGACCCTGAC ATGACAAGAG TTACTAACAG CCCCTCTCTC CAAGCTCACT TACAGGCTCT CTACTTAGTC CAGCACGAAG TCTGGAGACC TCTGGCGGCA GCCTACCAAG AACAACTGGA CCGACCGGTG GTACCTCACC CTTACCGAGT CGGCGACACA GTGTGGGTCC GCCGACACCA GACTAAGAAC CTAGAACCTC GCTGGAAAGG ACCTTACACA GTCCTGCTGA CCACCCCCAC CGCCCTCAAA GTAGACGGCA TCGCAGCTTG GATACACGCC GCCCACGTGA AGGCTGCCGA CCCCGGGGGT GGACCATCCT CTAGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAGGCAA TAGCATCACA AATTTCACAA ATAAGGCATT TTTTTCACTG CATTCTAGTT TTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCTCAAAT CCCTCGGAAG CTGCGCCTGT CTTAGGTTGG AGTGATACAT TTTTATCACT TTTACCCGTC TTTGGATTAG GCAGTAGCTC TGACGGCCCT CCTGTCTTAG GTTAGTGAAA AATGTCACTC TCTTACCCGT CATTGGCTGT CCAGCTTAGC TCGCAGGGGA GGTGGTCTGG ATCCGCCGGC ACCGGTGTAT ACGGGAATTC TTTACGAGGG TAGGAAGTGG TACGGAAAGT TGGTATAAGA CAAAAGTGTT GTGGAATTGA AGTTTACTCA AAAAATCAGC ACTCTTTTAT AGGCGCCCTG GTTTACATAA GCAAAGCTTA TACGTTCTCT ATCACTGATA GGGAGTAAAC TGGATATACG TTCTCTATCA CTGATAGGGA GTAAACTGTA GATACGTTCT CTATCACTGA TAGGGAGTAA ACTGGTCATA CGTTCTCTAT CACTGATAGG GAGTAAACTC CTTATACGTT CTCTATCACT GATAGGGAGT AAAGTCTGCA TACGTTCTCT ATCACTGATA GGGAGTAAAC TCTTCATACG TTCTCTATCA CTGATAGGGA GTAAACTCGA GGTGATAATT CCACGGGGTT GGGGTTGCGC CTTTTCCAAG GCAGCCCTGG GTTTGCGCAG GGACGCGGCT GCTCTGGGCG TGGTTCCGGG AAACGCAGCG GCGCCGACCC TGGGTCTCGC ACATTCTTCA CGTCCGTTCG CAGCGTCACC CGGATCTTCG CCGCTACCCT TGTGGGCCCC CCGGCGACGC TTCCTGCTCC GCCCCTAAGT CGGGAAGGTT CCTTGCGGTT CGCGGCGTGC CGGACGTGAC AAACGGAAGC CGCACGTCTC ACTAGTACCC TCGCAGACGG ACAGCGCCAG GGAGCAATGG CAGCGCGCCG ACCGCGATGG GCTGTGGCCA ATAGCGGCTG CTCAGCAGGG CGCGCCGAGA GCAGCGGCCG GGAAGGGGCG GTGCGGGAGG CGGGGTGTGG GGCGGTAGTG TGGGCCCTGT TCCTGCCCGC GCGGTGTTCC GCATTCTGCA AGCCTCCGGA GCGCACGTCG GCAGTCGGCT CCCTCGTTGA CCGAATCACC GACCTCTCTC CCCAGGGGGA TCATCGAATT ACCATGTCTA GACTGGACAA GAGCAAAGTC ATAAACTCTG CTCTGGAATT ACTCAATGGA GTCGGTATCG AAGGCCTGAC GACAAGGAAA CTCGCTCAAA AGCTGGGAGT TGAGCAGCCT ACCCTGTACT GGCACGTGAA GAACAAGCGG GCCCTGCTCG ATGCCCTGCC AATCGAGATG CTGGACAGGC ATCATACCCA CTCCTGCCCC CTGGAAGGCG AGTCATGGCA AGACTTTCTG CGGAACAACG CCAAGTCATA CCGCTGTGCT CTCCTCTCAC ATCGCGACGG GGCTAAAGTG CATCTCGGCA CCCGCCCAAC AGAGAAACAG TACGAAACCC TGGAAAATCA GCTCGCGTTC CTGTGTCAGC AAGGCTTCTC CCTGGAGAAC GCACTGTACG CTCTGTCCGC CGTGGGCCAC TTTACACTGG GCTGCGTATT GGAGGAACAG GAGCATCAAG TAGCAAAAGA GGAAAGAGAG ACACCTACCA CCGATTCTAT GCCCCCACTT CTGAAACAAG CAATTGAGCT GTTCGACCGG CAGGGAGCCG AACCTGCCTT CCTTTTCGGC CTGGAACTAA TCATATGTGG CCTGGAGAAA CAGCTAAAGT GCGAAAGCGG CGGGCCGACC GACGCCCTTG ACGATTTTGA CTTAGACATG CTCCCAGCCG ATGCCCTTGA CGACTTTGAC CTTGATATGC TGCCTGCTGA CGCTCTTGAC GATTTTGACC TTGACATGCT CCCCGGGTAA CTAAGTAAGA ATTGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAACG CGACCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCCGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGACC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGAACGCG TAATTCCCTG TTAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGATATTCTT AACTATGTTG CTCCTTTTAC GCTGTGTGGA TATGCTGCTT TAATGCCTCT GTATCATGCT ATTGCTTCCC GTACGGCTTT CGTTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCCGTCAA CGTGGCGTGG TGTGCTCTGT GTTTGCTGAC GCAACCCCCA CTGGCTGGGG CATTGCCACC ACCTGTCAAC TCCTTTCTGG GACTTTCGCT TTCCCCCTCC CGATCGCCAC GGCAGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTAGGT TGCTGGGCAC TGATAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCGATT CCTGCGGAGA TATCAGTGGT CCAGGCTCTA GTTTTGACTC AACAATATCA CCAGCTGAAG CCTATAGAGT ACGAGCCATA GATAAAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG GGAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCA GACATCAACA AAAGAGCCCA CAACCCCTCA CTCGGGGCGC CAGTCCTCCG ATTGACTGAG TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAC GCGTATGCCG CATAGTTAAG CCACCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGGCTCCG ACCGAAGCCG ACCCGGGCGG CCCCGCCGAC CCCGCACCCG CCCCCGAGGC CCACCGACTC TAGAGGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGGC GCGTCGAATT AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AA
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