pNiFty3-I-SEAP
$84.00
In stock
SKU
PL43647
100μg liquid plasmid(Instantly):$105
| name | pNiFty3-I-SEAP | another name | apNiFty3-I-SEAP |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Zeo | |
| Selective marker | Zeo | Fluorescent labeling | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGA ATCGTAACTA ACATACGCTC TCCATCAAAA CAAAACGAAA CAAAACAAAC TAGCAAAATA GGCTGTCCCC AGTGCAAGTG CAGGTGCCAG AACATTTCTC TATCGAAGGA TCTGCGATCG CTGAATTAGT TTCACTTTCC AGTTTCAGTT TCCAGTTTCA TTTTCCAGTT TCATTTTCCA GTTTCATTTT CCTGATATCC TGCAGGAGCT TGAATAAAAT GAATATTAGA AGCTGTTAGA ATAAGAGAAA ATGACAGAGG AAAACTGAAA GGGAGAACTG AAAGTGGGAA ATTCCTCTGA GGCAGAAAGG ACCATCCCTT ATAAATAGCA CAGGCCATGA AGGAAGATCA TTCTCACTGC AGCCTTTGAC AGCCTTTGCC TCATCTTGAA GCTTCTGCCT TCTCCCTCCT GTGAGTTTGG TAAGTCACTG ACTGTCTATG CCTGGGAAAG GGTGGGCAGG AGATGGGGCA GTGCAGGAAA AGTGGCACTA TGAACCCTGC AGCCCTAGGA ATGCATCTAG ACAATTGTAC TAACCTTCTT CTCTTTCCTC TCCTGACAGG TTGGTGTACA GTAGCTTCCA CCATGGTTCT GGGGCCCTGC ATGCTGCTGC TGCTGCTGCT GCTGGGCCTG AGGCTACAGC TCTCCCTGGG CATCATCCCA GTTGAGGAGG AGAACCCGGA CTTCTGGAAC CGCGAGGCAG CCGAGGCCCT GGGTGCCGCC AAGAAGCTGC AGCCTGCACA GACAGCCGCC AAGAACCTCA TCATCTTCCT GGGCGATGGG ATGGGGGTGT CTACGGTGAC AGCTGCCAGG ATCCTAAAAG GGCAGAAGAA GGACAAACTG GGGCCTGAGA TACCCCTGGC CATGGACCGC TTCCCATATG TGGCTCTGTC CAAGACATAC AATGTAGACA AACATGTGCC AGACAGTGGA GCCACAGCCA CGGCCTACCT GTGCGGGGTC AAGGGCAACT TCCAGACCAT TGGCTTGAGT GCAGCCGCCC GCTTTAACCA GTGCAACACG ACACGCGGCA ACGAGGTCAT CTCCGTGATG AATCGGGCCA AGAAAGCAGG GAAGTCAGTG GGAGTGGTAA CCACCACACG AGTGCAGCAC GCCTCGCCAG CCGGCACCTA CGCCCACACG GTGAACCGCA ACTGGTACTC GGACGCCGAC GTGCCTGCCT CGGCCCGCCA GGAGGGGTGC CAGGACATCG CTACGCAGCT CATCTCCAAC ATGGACATTG ACGTGATCCT AGGTGGAGGC CGAAAGTACA TGTTTCGCAT GGGAACCCCA GACCCTGAGT ACCCAGATGA CTACAGCCAA GGTGGGACCA GGCTGGACGG GAAGAATCTG GTGCAGGAAT GGCTGGCGAA GCGCCAGGGT GCCCGGTATG TGTGGAACCG CACTGAGCTC ATGCAGGCTT CCCTGGACCC GTCTGTGACC CATCTCATGG GTCTCTTTGA GCCTGGAGAC ATGAAATACG AGATCCACCG AGACTCCACA CTGGACCCCT CCCTGATGGA GATGACAGAG GCTGCCCTGC GCCTGCTGAG CAGGAACCCC CGCGGCTTCT TCCTCTTCGT GGAGGGTGGT CGCATCGACC ATGGTCATCA TGAAAGCAGG GCTTACCGGG CACTGACTGA GACGATCATG TTCGACGACG CCATTGAGAG GGCGGGCCAG CTCACCAGCG AGGAGGACAC GCTGAGCCTC GTCACTGCCG ACCACTCCCA CGTCTTCTCC TTCGGAGGCT ACCCCCTGCG AGGGAGCTCC ATCTTCGGGC TGGCCCCTGG CAAGGCCCGG GACAGGAAGG CCTACACGGT CCTCCTATAC GGAAACGGTC CAGGCTATGT GCTCAAGGAC GGCGCCCGGC CGGATGTTAC CGAGAGCGAG AGCGGGAGCC CCGAGTATCG GCAGCAGTCA GCAGTGCCCC TGGACGAAGA GACCCACGCA GGCGAGGACG TGGCGGTGTT CGCGCGCGGC CCGCAGGCGC ACCTGGTTCA CGGCGTGCAG GAGCAGACCT TCATAGCGCA CGTCATGGCC TTCGCCGCCT GCCTGGAGCC CTACACCGCC TGCGACCTGG CGCCCCCCGC CGGCACCACC GACGCCGCGC ACCCGGGGCG GTCCCGGTCC AAGCGTCTGG ATTGAAGCTA GCTGGCCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GAATTCTAAA ATACAGCATA GCAAAACTTT AACCTCCAAA TCAAGCCTCT ACTTGAATCC TTTTCTGAGG GATGAATAAG GCATAGGCAT CAGGGGCTGT TGCCAATGTG CATTAGCTGT TTGCAGCCTC ACCTTCTTTC ATGGAGTTTA AGATATAGTG TATTTTCCCA AGGTTTGAAC TAGCTCTTCA TTTCTTTATG TTTTAAATGC ACTGACCTCC CACATTCCCT TTTTAGTAAA ATATTCAGAA ATAATTTAAA TACATCATTG CAATGAAAAT AAATGTTTTT TATTAGGCAG AATCCAGATG CTCAAGGCCC TTCATAATAT CCCCCAGTTT AGTAGTTGGA CTTAGGGAAC AAAGGAACCT TTAATAGAAA TTGGACAGCA AGAAAGCGAG CTTCTAGCTT ATCCTCAGTC CTGCTCCTCT GCCACAAAGT GCACGCAGTT GCCGGCCGGG TCGCGCAGGG CGAACTCCCG CCCCCACGGC TGCTCGCCGA TCTCGGTCAT GGCCGGCCCG GAGGCGTCCC GGAAGTTCGT GGACACGACC TCCGACCACT CGGCGTACAG CTCGTCCAGG CCGCGCACCC ACACCCAGGC CAGGGTGTTG TCCGGCACCA CCTGGTCCTG GACCGCGCTG ATGAACAGGG TCACGTCGTC CCGGACCACA CCGGCGAAGT CGTCCTCCAC GAAGTCCCGG GAGAACCCGA GCCGGTCGGT CCAGAACTCG ACCGCTCCGG CGACGTCGCG CGCGGTGAGC ACCGGAACGG CACTGGTCAA CTTGGCCATG ATGGCCCTCC TATAGTGAGT CGTATTATAC TATGCCGATA TACTATGCCG ATGATTAATT GTCAACTACT GTTTGTAGGC GCCGGTCACA GCTTGGATCT GTAACGGCGC AGAACAGAAA ACGAAACAAA GACGTAGAGT TGAGCAAGCA GGGTCAGGCA AAGCGTGGAG AGCCGGCTGA GTCTAGGTAG GCTCCAAGGG AGCGCCGGAC AAAGGCCCGG TCTCGACCTG AGCTTTAAAC TTACCTAGAC GGCGGACGCA GTTCAGGAGG CACCACAGGC GGGAGGCGGC AGAACGCGAC TCAACCGGCG TGGATGGCGG CCTCAGGTAG GGCGGCGGGC GCGTGAAGGA GAGATGCGAG CCCCTCGAAG CTTCAGCTGT GTTCTGGCGG CAAACCCGTT GCGAAAAAGA ACGTTCACGG CGACTACTGC ACTTATATAC GGTTCTCCCC CACCCTCGGG AAAAAGGCGG AGCCAGTACA CGACATCACT TTCCCAGTTT ACCCCGCGCC ACCTTCTCTA GGCACCGGTT CAATTGCCGA CCCCTCCCCC CAACTTCTCG GGGACTGTGG GCGATGTGCG CTCTGCCCAC TGACACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGG CTAGTTAATT AACATTTAAA TCAGCGGCCG C
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