pCMV-3×HA-APC(human)-Neo
$171.00
In stock
SKU
PL43641
NM_000038.6;BTPS2; DESMD; DP2; DP2.5; DP3; GS; PPP1R46
100μg liquid plasmid(Instantly):$192
| name | pCMV-3×HA-APC(human)-Neo | another name | apCMV-3×HA-APC(human)-Neo |
| Insert Size (bp) | 8532bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCCACCAT GGCGGCGTAC CCATACGACG TACCTGATTA TGCAACTTAT CCTTATGATG TACCTGATTA TGCTAGCTAT CCTTATGATG TCCCAGACTA CGCATCAGCG GAAAAGCTTA TGGCTGCAGC TTCATATGAT CAGTTGTTAA AGCAAGTTGA GGCACTGAAG ATGGAGAACT CAAATCTTCG ACAAGAGCTA GAAGATAATT CCAATCATCT TACAAAACTG GAAACTGAGG CATCTAATAT GAAGGAAGTA CTTAAACAAC TACAAGGAAG TATTGAAGAT GAAGCTATGG CTTCTTCTGG ACAGATTGAT TTATTAGAGC GTCTTAAAGA GCTTAACTTA GATAGCAGTA ATTTCCCTGG AGTAAAACTG CGGTCAAAAA TGTCCCTCCG TTCTTATGGA AGCCGGGAAG GATCTGTATC AAGCCGTTCT GGAGAGTGCA GTCCTGTTCC TATGGGTTCA TTTCCAAGAA GAGGGTTTGT AAATGGAAGC AGAGAAAGTA CTGGATATTT AGAAGAACTT GAGAAAGAGA GGTCATTGCT TCTTGCTGAT CTTGACAAAG AAGAAAAGGA AAAAGACTGG TATTACGCTC AACTTCAGAA TCTCACTAAA AGAATAGATA GTCTTCCTTT AACTGAAAAT TTTTCCTTAC AAACAGATAT GACCAGAAGG CAATTGGAAT ATGAAGCAAG GCAAATCAGA GTTGCGATGG AAGAACAACT AGGTACCTGC CAGGATATGG AAAAACGAGC ACAGCGAAGA ATAGCCAGAA TTCAGCAAAT CGAAAAGGAC ATACTTCGTA TACGACAGCT TTTACAGTCC CAAGCAACAG AAGCAGAGAG GTCATCTCAG AACAAGCATG AAACCGGCTC ACATGATGCT GAGCGGCAGA ATGAAGGTCA AGGAGTGGGA GAAATCAACA TGGCAACTTC TGGTAATGGT CAGGGTTCAA CTACACGAAT GGACCATGAA ACAGCCAGTG TTTTGAGTTC TAGTAGCACA CACTCTGCAC CTCGAAGGCT GACAAGTCAT CTGGGAACCA AGGTGGAAAT GGTGTATTCA TTGTTGTCAA TGCTTGGTAC TCATGATAAG GATGATATGT CGCGAACTTT GCTAGCTATG TCTAGCTCCC AAGACAGCTG TATATCCATG CGACAGTCTG GATGTCTTCC TCTCCTCATC CAGCTTTTAC ATGGCAATGA CAAAGACTCT GTATTGTTGG GAAATTCCCG GGGCAGTAAA GAGGCTCGGG CCAGGGCCAG TGCAGCACTC CACAACATCA TTCACTCACA GCCTGATGAC AAGAGAGGCA GGCGTGAAAT CCGAGTCCTT CATCTTTTGG AACAGATACG CGCTTACTGT GAAACCTGTT GGGAGTGGCA GGAAGCTCAT GAACCAGGCA TGGACCAGGA CAAAAATCCA ATGCCAGCTC CTGTTGAACA TCAGATCTGT CCTGCTGTGT GTGTTCTAAT GAAACTTTCA TTTGATGAAG AGCATAGACA TGCAATGAAT GAACTAGGGG GACTACAGGC CATTGCAGAA TTATTGCAAG TGGACTGTGA AATGTATGGG CTTACTAATG ACCACTACAG TATTACACTA AGACGATATG CTGGAATGGC TTTGACAAAC TTGACTTTTG GAGATGTAGC CAACAAGGCT ACGCTATGCT CTATGAAAGG CTGCATGAGA GCACTTGTGG CCCAACTAAA ATCTGAAAGT GAAGACTTAC AGCAGGTTAT TGCGAGTGTT TTGAGGAATT TGTCTTGGCG AGCAGATGTA AATAGTAAAA AGACGTTGCG AGAAGTTGGA AGTGTGAAAG CATTGATGGA ATGTGCTTTA GAAGTTAAAA AGGAATCAAC CCTCAAAAGC GTATTGAGTG CCTTATGGAA TTTGTCAGCA CATTGCACTG AGAATAAAGC TGATATATGT GCTGTAGATG GTGCACTTGC ATTTTTGGTT GGCACTCTTA CTTACCGGAG CCAGACAAAC ACTTTAGCCA TTATTGAAAG TGGAGGTGGG ATATTACGGA ATGTGTCCAG CTTGATAGCT ACAAATGAGG ACCACAGGCA AATCCTAAGA GAGAACAACT GTCTACAAAC TTTATTACAA CACTTAAAAT CTCATAGTTT GACAATAGTC AGTAATGCAT GTGGAACTTT GTGGAATCTC TCAGCAAGAA ATCCTAAAGA CCAGGAAGCA TTATGGGACA TGGGGGCAGT TAGCATGCTC AAGAACCTCA TTCATTCAAA GCACAAAATG ATTGCTATGG GAAGTGCTGC AGCTTTAAGG AATCTCATGG CAAATAGGCC TGCGAAGTAC AAGGATGCCA ATATTATGTC TCCTGGCTCA AGCTTGCCAT CTCTTCATGT TAGGAAACAA AAAGCCCTAG AAGCAGAATT AGATGCTCAG CACTTATCAG AAACTTTTGA CAATATAGAC AATTTAAGTC CCAAGGCATC TCATCGTAGT AAGCAGAGAC ACAAGCAAAG TCTCTATGGT GATTATGTTT TTGACACCAA TCGACATGAT GATAATAGGT CAGACAATTT TAATACTGGC AACATGACTG TCCTTTCACC ATATTTGAAT ACTACAGTGT TACCCAGCTC CTCTTCATCA AGAGGAAGCT TAGATAGTTC TCGTTCTGAA AAAGATAGAA GTTTGGAGAG AGAACGCGGA ATTGGTCTAG GCAACTACCA TCCAGCAACA GAAAATCCAG GAACTTCTTC AAAGCGAGGT TTGCAGATCT CCACCACTGC AGCCCAGATT GCCAAAGTCA TGGAAGAAGT GTCAGCCATT CATACCTCTC AGGAAGACAG AAGTTCTGGG TCTACCACTG AATTACATTG TGTGACAGAT GAGAGAAATG CACTTAGAAG AAGCTCTGCT GCCCATACAC ATTCAAACAC TTACAATTTC ACTAAGTCGG AAAATTCAAA TAGGACATGT TCTATGCCTT ATGCCAAATT AGAATACAAG AGATCTTCAA ATGATAGTTT AAATAGTGTC AGTAGTAGTG ATGGTTATGG TAAAAGAGGT CAAATGAAAC CCTCGATTGA ATCCTATTCT GAAGATGATG AAAGTAAGTT TTGCAGTTAT GGTCAATACC CAGCCGACCT AGCCCATAAA ATACATAGTG CAAATCATAT GGATGATAAT GATGGAGAAC TAGATACACC AATAAATTAT AGTCTTAAAT ATTCAGATGA GCAGTTGAAC TCTGGAAGGC AAAGTCCTTC ACAGAATGAA AGATGGGCAA GACCCAAACA CATAATAGAA GATGAAATAA AACAAAGTGA GCAAAGACAA TCAAGGAATC AAAGTACAAC TTATCCTGTT TATACTGAGA GCACTGATGA TAAACACCTC AAGTTCCAAC CACATTTTGG ACAGCAGGAA TGTGTTTCTC CATACAGGTC ACGGGGAGCC AATGGTTCAG AAACAAATCG AGTGGGTTCT AATCATGGAA TTAATCAAAA TGTAAGCCAG TCTTTGTGTC AAGAAGATGA CTATGAAGAT GATAAGCCTA CCAATTATAG TGAACGTTAC TCTGAAGAAG AACAGCATGA AGAAGAAGAG AGACCAACAA ATTATAGCAT AAAATATAAT GAAGAGAAAC GTCATGTGGA TCAGCCTATT GATTATAGTT TAAAATATGC CACAGATATT CCTTCATCAC AGAAACAGTC ATTTTCATTC TCAAAGAGTT CATCTGGACA AAGCAGTAAA ACCGAACATA TGTCTTCAAG CAGTGAGAAT ACGTCCACAC CTTCATCTAA TGCCAAGAGG CAGAATCAGC TCCATCCAAG TTCTGCACAG AGTAGAAGTG GTCAGCCTCA AAAGGCTGCC ACTTGCAAAG TTTCTTCTAT TAACCAAGAA ACAATACAGA CTTATTGTGT AGAAGATACT CCAATATGTT TTTCAAGATG TAGTTCATTA TCATCTTTGT CATCAGCTGA AGATGAAATA GGATGTAATC AGACGACACA GGAAGCAGAT TCTGCTAATA CCCTGCAAAT AGCAGAAATA AAAGAAAAGA TTGGAACTAG GTCAGCTGAA GATCCTGTGA GCGAAGTTCC AGCAGTGTCA CAGCACCCTA GAACCAAATC CAGCAGACTG CAGGGTTCTA GTTTATCTTC AGAATCAGCC AGGCACAAAG CTGTTGAATT TTCTTCAGGA GCGAAATCTC CCTCCAAAAG TGGTGCTCAG ACACCCAAAA GTCCACCTGA ACACTATGTT CAGGAGACCC CACTCATGTT TAGCAGATGT ACTTCTGTCA GTTCACTTGA TAGTTTTGAG AGTCGTTCGA TTGCCAGCTC CGTTCAGAGT GAACCATGCA GTGGAATGGT AAGTGGCATT ATAAGCCCCA GTGATCTTCC AGATAGCCCT GGACAAACCA TGCCACCAAG CAGAAGTAAA ACACCTCCAC CACCTCCTCA AACAGCTCAA ACCAAGCGAG AAGTACCTAA AAATAAAGCA CCTACTGCTG AAAAGAGAGA GAGTGGACCT AAGCAAGCTG CAGTAAATGC TGCAGTTCAG AGGGTCCAGG TTCTTCCAGA TGCTGATACT TTATTACATT TTGCCACGGA AAGTACTCCA GATGGATTTT CTTGTTCATC CAGCCTGAGT GCTCTGAGCC TCGATGAGCC ATTTATACAG AAAGATGTGG AATTAAGAAT AATGCCTCCA GTTCAGGAAA ATGACAATGG GAATGAAACA GAATCAGAGC AGCCTAAAGA ATCAAATGAA AACCAAGAGA AAGAGGCAGA AAAAACTATT GATTCTGAAA AGGACCTATT AGATGATTCA GATGATGATG ATATTGAAAT ACTAGAAGAA TGTATTATTT CTGCCATGCC AACAAAGTCA TCACGTAAAG CAAAAAAGCC AGCCCAGACT GCTTCAAAAT TACCTCCACC TGTGGCAAGG AAACCAAGTC AGCTGCCTGT GTACAAACTT CTACCATCAC AAAACAGGTT GCAACCCCAA AAGCATGTTA GTTTTACACC GGGGGATGAT ATGCCACGGG TGTATTGTGT TGAAGGGACA CCTATAAACT TTTCCACAGC TACATCTCTA AGTGATCTAA CAATCGAATC CCCTCCAAAT GAGTTAGCTG CTGGAGAAGG AGTTAGAGGA GGGGCACAGT CAGGTGAATT TGAAAAACGA GATACCATTC CTACAGAAGG CAGAAGTACA GATGAGGCTC AAGGAGGAAA AACCTCATCT GTAACCATAC CTGAATTGGA TGACAATAAA GCAGAGGAAG GTGATATTCT TGCAGAATGC ATTAATTCTG CTATGCCCAA AGGGAAAAGT CACAAGCCTT TCCGTGTGAA AAAGATAATG GACCAGGTCC AGCAAGCATC TGCGTCTTCT TCTGCACCCA ACAAAAATCA GTTAGATGGT AAGAAAAAGA AACCAACTTC ACCAGTAAAA CCTATACCAC AAAATACTGA ATATAGGACA CGTGTAAGAA AAAATGCAGA CTCAAAAAAT AATTTAAATG CTGAGAGAGT TTTCTCAGAC AACAAAGATT CAAAGAAACA GAATTTGAAA AATAATTCCA AGGTCTTCAA TGATAAGCTC CCAAATAATG AAGATAGAGT CAGAGGAAGT TTTGCTTTTG ATTCACCTCA TCATTACACG CCTATTGAAG GAACTCCTTA CTGTTTTTCA CGAAATGATT CTTTGAGTTC TCTAGATTTT GATGATGATG ATGTTGACCT TTCCAGGGAA AAGGCTGAAT TAAGAAAGGC AAAAGAAAAT AAGGAATCAG AGGCTAAAGT TACCAGCCAC ACAGAACTAA CCTCCAACCA ACAATCAGCT AATAAGACAC AAGCTATTGC AAAGCAGCCA ATAAATCGAG GTCAGCCTAA ACCCATACTT CAGAAACAAT CCACTTTTCC CCAGTCATCC AAAGACATAC CAGACAGAGG GGCAGCAACT GATGAAAAGT TACAGAATTT TGCTATTGAA AATACTCCGG TTTGCTTTTC TCATAATTCC TCTCTGAGTT CTCTCAGTGA CATTGACCAA GAAAACAACA ATAAAGAAAA TGAACCTATC AAAGAGACTG AGCCCCCTGA CTCACAGGGA GAACCAAGTA AACCTCAAGC ATCAGGCTAT GCTCCTAAAT CATTTCATGT TGAAGATACC CCAGTTTGTT TCTCAAGAAA CAGTTCTCTC AGTTCTCTTA GTATTGACTC TGAAGATGAC CTGTTGCAGG AATGTATAAG CTCCGCAATG CCAAAAAAGA AAAAGCCTTC AAGACTCAAG GGTGATAATG AAAAACATAG TCCCAGAAAT ATGGGTGGCA TATTAGGTGA AGATCTGACA CTTGATTTGA AAGATATACA GAGACCAGAT TCAGAACATG GTCTATCCCC TGATTCAGAA AATTTTGATT GGAAAGCTAT TCAGGAAGGT GCAAATTCCA TAGTAAGTAG TTTACATCAA GCTGCTGCTG CTGCATGTTT ATCTAGACAA GCTTCGTCTG ATTCAGATTC CATCCTTTCC CTGAAATCAG GAATCTCTCT GGGATCACCA TTTCATCTTA CACCTGATCA AGAAGAAAAA CCCTTTACAA GTAATAAAGG CCCACGAATT CTAAAACCAG GGGAGAAAAG TACATTGGAA ACTAAAAAGA TAGAATCTGA AAGTAAAGGA ATCAAAGGAG GAAAAAAAGT TTATAAAAGT TTGATTACTG GAAAAGTTCG ATCTAATTCA GAAATTTCAG GCCAAATGAA ACAGCCCCTT CAAGCAAACA TGCCTTCAAT CTCTCGAGGC AGGACAATGA TTCATATTCC AGGAGTTCGA AATAGCTCCT CAAGTACAAG TCCTGTTTCT AAAAAAGGCC CACCCCTTAA GACTCCAGCC TCCAAAAGCC CTAGTGAAGG TCAAACAGCC ACCACTTCTC CTAGAGGAGC CAAGCCATCT GTGAAATCAG AATTAAGCCC TGTTGCCAGG CAGACATCCC AAATAGGTGG GTCAAGTAAA GCACCTTCTA GATCAGGATC TAGAGATTCG ACCCCTTCAA GACCTGCCCA GCAACCATTA AGTAGACCTA TACAGTCTCC TGGCCGAAAC TCAATTTCCC CTGGTAGAAA TGGAATAAGT CCTCCTAACA AATTATCTCA ACTTCCAAGG ACATCATCCC CTAGTACTGC TTCAACTAAG TCCTCAGGTT CTGGAAAAAT GTCATATACA TCTCCAGGTA GACAGATGAG CCAACAGAAC CTTACCAAAC AAACAGGTTT ATCCAAGAAT GCCAGTAGTA TTCCAAGAAG TGAGTCTGCC TCCAAAGGAC TAAATCAGAT GAATAATGGT AATGGAGCCA ATAAAAAGGT AGAACTTTCT AGAATGTCTT CAACTAAATC AAGTGGAAGT GAATCTGATA GATCAGAAAG ACCTGTATTA GTACGCCAGT CAACTTTCAT CAAAGAAGCT CCAAGCCCAA CCTTAAGAAG AAAATTGGAG GAATCTGCTT CATTTGAATC TCTTTCTCCA TCATCTAGAC CAGCTTCTCC CACTAGGTCC CAGGCACAAA CTCCAGTTTT AAGTCCTTCC CTTCCTGATA TGTCTCTATC CACACATTCG TCTGTTCAGG CTGGTGGATG GCGAAAACTC CCACCTAATC TCAGTCCCAC TATAGAGTAT AATGATGGAA GACCAGCAAA GCGCCATGAT ATTGCACGGT CTCATTCTGA AAGTCCTTCT AGACTTCCAA TCAATAGGTC AGGAACCTGG AAACGTGAGC ACAGCAAACA TTCATCATCC CTTCCTCGAG TAAGCACTTG GAGAAGAACT GGAAGTTCAT CTTCAATTCT TTCTGCTTCA TCAGAATCCA GTGAAAAAGC AAAAAGTGAG GATGAAAAAC ATGTGAACTC TATTTCAGGA ACCAAACAAA GTAAAGAAAA CCAAGTATCC GCAAAAGGAA CATGGAGAAA AATAAAAGAA AATGAATTTT CTCCCACAAA TAGTACTTCT CAGACCGTTT CCTCAGGTGC TACAAATGGT GCTGAATCAA AGACTCTAAT TTATCAAATG GCACCTGCTG TTTCTAAAAC AGAGGATGTT TGGGTGAGAA TTGAGGACTG TCCCATTAAC AATCCTAGAT CTGGAAGATC TCCCACAGGT AATACTCCCC CGGTGATTGA CAGTGTTTCA GAAAAGGCAA ATCCAAACAT TAAAGATTCA AAAGATAATC AGGCAAAACA AAATGTGGGT AATGGCAGTG TTCCCATGCG TACCGTGGGT TTGGAAAATC GCCTGAACTC CTTTATTCAG GTGGATGCCC CTGACCAAAA AGGAACTGAG ATAAAACCAG GACAAAATAA TCCTGTCCCT GTATCAGAGA CTAATGAAAG TTCTATAGTG GAACGTACCC CATTCAGTTC TAGCAGCTCA AGCAAACACA GTTCACCTAG TGGGACTGTT GCTGCCAGAG TGACTCCTTT TAATTACAAC CCAAGCCCTA GGAAAAGCAG CGCAGATAGC ACTTCAGCTC GGCCATCTCA GATCCCAACT CCAGTGAATA ACAACACAAA GAAGCGAGAT TCCAAAACTG ACAGCACAGA ATCCAGTGGA ACCCAAAGTC CTAAGCGCCA TTCTGGGTCT TACCTTGTGA CATCTGTTTA AGAATTCTGC AGATATCCAG CACAGTGGCG GCCGCTCGAG TCTAGAGGGC CCGTTTAAAC CCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTC
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