NM_016333.4;300-KD; Cwc21; CWF21; HSPC075; SRL300;SRm300
100μg liquid plasmid(Instantly):$192
| name | pLV3-CMV-SRRM2(human)-3×FLAG-Puro | another name | apLV3-CMV-SRRM2(human)-3×FLAG-Puro |
| Insert Size (bp) | 8259bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG TGACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA GAAGATTCTA GAGCTAGCGA ATTCGCCACC ATGTACAACG GGATCGGGCT GCCGACGCCC CGGGGCAGCG GCACCAACGG CTACGTCCAG CGCAACCTGT CCCTGGTGCG GGGCCGCCGG GGTGAGCGGC CTGACTACAA GGGAGAGGAG GAACTGCGGC GCCTGGAGGC TGCCCTGGTG AAGCGGCCTA ATCCTGACAT CCTGGACCAC GAGCGCAAGC GGCGCGTCGA GCTGCGATGC CTCGAGCTGG AGGAGATGAT GGAAGAGCAG GGGTACGAGG AACAGCAAAT TCAGGAAAAA GTGGCGACCT TTCGACTCAT GTTGCTGGAG AAGGATGTGA ACCCTGGGGG CAAGGAGGAG ACCCCAGGGC AGAGGCCAGC GGTCACGGAG ACTCACCAGT TGGCAGAATT AAATGAGAAG AAGAATGAAA GACTCCGTGC TGCCTTTGGC ATCAGTGATT CTTACGTAGA TGGCAGCTCT TTTGATCCTC AGCGTCGTGC CCGAGAAGCT AAACAACCAG CTCCTGAGCC TCCCAAACCT TACAGCCTTG TTCGGGAGTC TAGCAGTTCT CGCTCACCAA CCCCAAAGCA GAAGAAGAAG AAAAAGAAGA AAGATAGAGG ACGCAGGTCA GAGAGCAGCT CTCCTCGACG GGAGAGAAAG AAAAGCTCAA AGAAGAAGAA GCACAGGTCA GAATCTGAGT CCAAGAAACG TAAGCATAGG TCTCCCACTC CAAAGAGCAA ACGTAAATCT AAGGACAAAA AGCGAAAGCG GTCTCGAAGT ACAACACCAG CCCCCAAGAG CCGCCGGGCC CACCGTTCAA CTTCTGCTGA CTCTGCTTCC TCCTCCGATA CTTCCCGCAG TCGGTCTCGA AGTGCTGCAG CTAAAACTCA TACAACTGCC TTGGCTGGGC GAAGTCCTTC CCCTGCTTCA GGGCGACGCG GGGAGGGAGA TGCGCCTTTC AGTGAACCAG GTACTACCAG CACACAACGG CCTAGTAGCC CGGAGACTGC TACGAAACAG CCTAGCAGCC CTTATGAAGA CAAAGATAAA GACAAGAAGG AGAAATCTGC AACTCGACCT AGCCCCTCTC CGGAAAGGAG CAGCACAGGC CCAGAACCAC CTGCTCCCAC TCCGCTCCTT GCTGAGCGAC ATGGCGGCTC CCCACAACCC CTTGCAACCA CCCCCTTAAG CCAGGAGCCA GTGAACCCCC CATCTGAGGC CTCTCCAACT CGGGACCGTT CACCACCTAA GTCTCCCGAG AAACTTCCCC AGTCTTCTTC CTCAGAGAGC AGCCCACCAT CCCCTCAACC TACCAAAGTT TCTCGGCATG CCAGCTCTTC CCCAGAAAGT CCTAAACCTG CTCCAGCTCC AGGGTCCCAC CGAGAGATTT CTTCTTCTCC CACATCTAAG AATCGCTCAC ATGGCCGAGC AAAACGGGAT AAATCACATT CTCATACCCC CTCCCGTAGG ATGGGGAGGT CCCGTAGCCC TGCCACCGCT AAGAGAGGGC GATCTCGGTC TCGAACCCCT ACCAAGAGAG GTCATTCTCG ATCCCGATCT CCCCAGTGGC GTAGGTCCAG GTCTGCACAG AGGTGGGGAA GATCTAGAAG CCCCCAGCGA CGTGGCCGCT CTAGGTCTCC TCAGCGACCA GGCTGGTCTA GGAGCAGAAA TACCCAGAGA AGAGGCAGGT CTAGGTCAGC AAGGCGAGGG AGGTCCCACT CTAGATCCCC AGCCACTAGG GGTAGATCTC GTTCTAGAAC ACCAGCCCGC CGGGGCAGGT CCCGCTCTAG AACACCTGCC AGGCGGAGAT CACGATCCAG AACTCCCACC AGGCGTAGGT CTCGGTCTAG AACACCAGCC CGGAGGGGCA GGTCTCGGTC TAGAACACCT GCTAGGCGCA GATCTAGGAC CCGATCACCA GTACGACGCA GGTCTCGTAG TAGATCACCA GCCAGGAGAA GTGGCAGGTC ACGCTCTAGA ACCCCAGCTA GACGTGGCCG CTCACGCTCC AGAACCCCAG CCAGACGTGG CCGCTCACGC TCTAGAACCC CAGCTAGACG CAGTGGTCGC TCACGCTCCA GAACACCAGC CAGGAGAGGG AGGTCTCGGT CTAGGACACC AAGACGAGGA AGATCCCGCA GTAGAAGCTT AGTTAGACGT GGAAGATCTC ACTCTAGAAC ACCTCAAAGA AGAGGCAGAT CTGGCTCATC TTCAGAGCGG AAAAACAAAT CCAGAACATC TCAAAGAAGA AGCAGGTCCA ATTCAAGCCC AGAAATGAAG AAATCTCGCA TTTCTTCAAG GCGGAGCAGG TCTCTCTCTT CACCACGGTC CAAAGCAAAA TCTCGCTTGT CTTTGAGGCG CAGCCTTTCA GGGTCTTCCC CATGCCCTAA ACAAAAGTCA CAGACACCAC CCAGGCGCAG TCGCTCTGGA TCCTCCCAAC CTAAAGCTAA ATCTAGAACG CCACCCAGAC GCAGTCGCTC CAGTTCTTCT CCGCCACCTA AACAGAAATC TAAGACACCA TCAAGACAAA GTCATTCCAG TTCATCTCCT CATCCTAAAG TGAAATCTGG AACACCACCG AGGCAAGGGT CCATAACAAG TCCCCAGGCC AATGAGCAAT CTGTAACGCC ACAGAGACGG AGCTGTTTTG AATCATCACC TGACCCTGAG TTGAAATCTA GGACCCCTTC TAGACATAGC TGCTCAGGGT CCTCTCCTCC TAGAGTGAAA TCTAGCACAC CTCCCAGACA GAGCCCATCT AGGTCATCAT CTCCACAACC CAAAGTGAAG GCAATAATAT CACCAAGACA AAGAAGCCAT TCTGGCTCCT CTTCTCCAAG TCCTAGTAGG GTGACGTCGA GAACAACTCC ACGGCGAAGC AGATCAGTAT CTCCCTGCTC CAATGTGGAA TCCAGATTGT TGCCAAGATA CAGTCATTCT GGGTCCTCCT CACCAGATAC CAAAGTGAAA CCTGAAACAC CGCCAAGACA AAGTCACTCA GGGTCTATTT CACCATACCC CAAAGTAAAG GCCCAAACTC CACCGGGGCC AAGTCTTTCT GGATCAAAGT CACCATGTCC CCAAGAGAAG TCTAAAGACT CACTAGTTCA AAGTTGCCCT GGATCCCTCT CTCTCTGTGC AGGAGTAAAA TCTAGCACAC CACCAGGCGA GAGCTATTTT GGTGTCTCAT CTCTGCAACT GAAAGGACAA TCTCAAACTT CACCAGACCA CAGATCTGAT ACTTCAAGTC CAGAAGTGAG ACAGAGTCAT TCAGAATCAC CATCTCTGCA GAGCAAATCT CAAACATCAC CTAAGGGAGG TCGGTCCAGG TCTTCATCTC CAGTCACTGA GCTGGCATCC AGATCTCCAA TAAGACAAGA TAGAGGTGAG TTCTCAGCGA GTCCTATGTT GAAATCTGGA ATGTCTCCTG AGCAGAGCAG GTTCCAGTCT GACTCTTCTT CATATCCTAC AGTGGACTCG AATTCTCTCT TGGGGCAGAG TAGATTGGAG ACTGCTGAAT CAAAAGAGAA AATGGCCTTA CCCCCTCAGG AGGATGCTAC TGCATCACCT CCTAGACAGA AAGACAAATT TAGTCCCTTT CCAGTACAGG ATAGGCCTGA GTCTTCACTG GTATTCAAAG ACACACTTAG AACCCCGCCA AGGGAAAGAA GTGGTGCTGG GTCATCTCCA GAAACAAAAG AGCAAAATAG TGCATTGCCT ACGTCAAGCC AAGATGAAGA GTTAATGGAG GTGGTAGAGA AGTCTGAAGA ACCCGCAGGC CAAATCCTGT CTCATTTGTC TTCAGAACTT AAAGAAATGT CCACAAGTAA CTTTGAATCA TCTCCTGAAG TAGAAGAAAG GCCTGCTGTG TCTTTGACTC TTGATCAGAG CCAGTCACAG GCTTCTTTGG AAGCAGTAGA AGTCCCTTCA ATGGCCTCAT CTTGGGGTGG GCCACATTTT TCTCCAGAAC ATAAAGAACT GTCTAACTCC CCACTCAGGG AGAACAGCTT TGGATCACCT TTAGAATTTA GAAACTCAGG CCCACTTGGT ACAGAAATGA ATACTGGATT TTCTTCTGAG GTTAAAGAAG ATTTGAATGG ACCGTTTCTT AATCAGCTGG AAACAGATCC ATCTCTAGAC ATGAAAGAAC AATCGACAAG ATCCTCTGGA CACAGCAGTT CTGAGTTATC CCCAGATGCA GTGGAAAAGG CAGGGATGTC TTCAAATCAG AGCATCTCTT CACCTGTGCT TGATGCTGTA CCCAGAACAC CCTCGAGAGA AAGAAGTAGT TCTGCATCTT CTCCTGAAAT GAAAGATGGT TTACCCAGAA CTCCATCAAG GAGAAGCAGG TCTGGGTCTT CTCCAGGACT TAGAGATGGG TCTGGGACTC CCTCGAGGCA CAGCCTGTCT GGGTCCTCTC CTGGAATGAA AGATATACCT AGAACGCCAT CTAGAGGGAG AAGCGAATGT GATTCTTCCC CAGAACCGAA AGCTTTGCCT CAGACTCCTA GGCCGAGGAG TCGTTCTCCA TCATCCCCAG AGCTCAACAA CAAGTGTCTT ACCCCCCAGA GAGAAAGAAG CGGGTCAGAA TCATCAGTTG ATCAGAAAAC TGTGGCTCGG ACTCCCCTGG GGCAGAGAAG TCGTTCGGGA TCCTCTCAAG AACTTGATGT GAAACCCAGT GCATCCCCTC AGGAAAGAAG TGAGTCAGAC TCTTCTCCAG ATTCTAAAGC CAAGACAAGA ACCCCACTTC GGCAGAGGAG TCGGTCTGGA TCATCTCCAG AGGTTGACAG CAAATCTCGA CTATCCCCTC GGCGCAGTAG GTCTGGTTCC TCCCCTGAAG TGAAAGATAA GCCAAGAGCA GCACCCAGGG CACAGAGTGG TTCTGATTCC TCTCCTGAAC CTAAAGCTCC AGCCCCTCGG GCCCTTCCCA GACGAAGCAG ATCAGGTTCA TCAAGCAAAG GCAGAGGCCC TTCTCCTGAA GGAAGCAGCA GTACCGAGTC CTCTCCTGAA CATCCGCCCA AATCCAGAAC TGCTCGCAGA GGTTCCAGGT CATCACCAGA GCCCAAGACC AAGTCTCGTA CACCACCTCG ACGTCGCAGC TCTCGATCAT CTCCGGAGCT AACAAGGAAG GCCAGACTGT CCCGTAGAAG CCGCTCTGCC TCATCCTCAC CAGAAACTCG CTCTAGAACT CCCCCAAGGC ACCGGAGAAG TCCCTCAGTG TCTTCCCCGG AGCCAGCCGA AAAATCGAGG TCTTCACGCC GACGGCGCTC AGCTTCATCT CCACGCACTA AGACAACCTC AAGGAGAGGC CGCTCTCCTT CGCCAAAGCC TCGTGGACTC CAGAGGTCCC GTTCCCGCTC AAGGAGAGAG AAAACAAGAA CAACCCGACG TCGAGATAGG TCTGGATCTT CTCAGTCAAC CTCTCGGCGA AGACAGCGGA GCCGGTCAAG GTCGCGGGTT ACTCGGCGGC GGAGGGGAGG CTCTGGTTAT CACTCAAGGT CACCTGCCCG GCAGGAAAGT TCCCGGACCT CCTCTCGACG CCGAAGAGGC CGCTCTCGGA CACCCCCAAC CAGTCGGAAG CGTTCTCGCT CACGCACATC ACCAGCCCCG TGGAAACGCT CTAGATCTCG AGCCTCTCCA GCCACTCACC GGCGATCCAG GTCCAGAACC CCCCTGATAA GCCGACGTAG GTCCAGATCT CGAACTTCAC CAGTCAGCCG GAGACGGTCA AGGTCCAGGA CTTCAGTGAC TCGACGAAGA TCCCGGTCAA GAGCATCCCC AGTGAGCAGA AGGCGATCCA GATCCAGAAC GCCACCAGTA ACCCGCCGTC GTTCAAGGTC TAGAACGCCA ACAACACGCC GCCGCTCCCG TTCTAGAACT CCACCAGTGA CTCGCAGAAG GTCCAGATCC AGGACTCCAC CAGTAACCAG GAGGCGATCT CGAAGCAGAA CTTCGCCTAT CACTCGCAGA AGATCAAGAT CCAGAACATC TCCGGTCACC CGAAGGAGAT CTCGATCTCG CACATCTCCA GTAACTCGAA GAAGGTCCCG CTCTCGAACC TCACCAGTGA CACGCCGCCG CTCTAGGTCC CGGACACCTC CAGCTATTCG GCGCCGCTCT AGATCTCGAA CGCCACTGTT ACCACGCAAA CGTTCTCGAA GTCGCTCACC ACTTGCTATC CGCCGCCGCT CCAGATCCCG TACTCCACGA ACAGCTCGGG GTAAACGGTC CTTAACAAGA TCTCCTCCAG CCATCCGCAG GCGTTCTGCA TCTGGAAGTA GTTCTGATCG TTCACGATCT GCTACTCCTC CAGCAACAAG AAATCATTCT GGTTCACGGA CACCTCCAGT AGCACTCAAC AGTTCCAGAA TGAGCTGCTT CAGTCGTCCT AGCATGTCCC CAACACCTCT TGATCGCTGC AGATCACCTG GAATGCTTGA ACCCCTTGGC AGCTCTAGAA CACCCATGTC TGTCCTGCAG CAAGCCGGCG GCTCCATGAT GGATGGTCCA GGTCCCCGAA TACCTGACCA CCAGAGAACA TCTGTGCCAG AAAATCATGC TCAGTCCAGG ATTGCACTTG CCCTGACAGC TATCAGTCTT GGCACCGCTC GGCCTCCTCC GTCCATGTCT GCTGCTGGCC TTGCTGCAAG AATGTCCCAG GTTCCAGCCC CGGTGCCTCT CATGAGTCTC AGAACCGCAC CAGCAGCCAA CCTTGCCAGC AGGATTCCTG CAGCCTCTGC GGCAGCCATG AACCTAGCCA GCGCCAGGAC ACCTGCCATT CCAACAGCAG TGAACCTGGC TGACTCTCGA ACGCCAGCTG CAGCAGCGGC CATGAACTTG GCCAGCCCCA GAACAGCGGT GGCACCTTCG GCTGTGAACC TGGCTGACCC TCGCACTCCC ACAGCCCCAG CTGTGAACCT AGCAGGGGCC AGAACCCCAG CTGCCTTGGC AGCTCTGAGT CTCACAGGCT CTGGCACACC ACCAACTGCT GCAAACTATC CCTCCAGCTC CAGAACACCA CAGGCTCCAG CCTCTGCAAA CCTGGTGGGT CCTCGGTCTG CACATGCCAC AGCTCCTGTG AATATTGCCG GCTCCAGAAC CGCCGCAGCC TTGGCCCCCG CGAGCCTCAC CAGTGCTAGG ATGGCTCCAG CATTGTCTGG TGCAAACCTC ACCAGCCCCA GGGTGCCCCT TTCTGCCTAC GAGCGTGTCA GTGGCAGAAC CTCACCACCG CTCCTTGACC GAGCTAGGTC CAGAACACCA CCGTCTGCCC CAAGCCAATC TAGGATGACC TCTGAACGGG CTCCCTCCCC TTCCTCTAGA ATGGGCCAGG CTCCTTCACA GTCTCTTCTC CCTCCAGCAC AGGATCAGCC GAGGTCTCCT GTGCCTTCTG CTTTTTCAGA CCAATCCCGT TGTTTGATTG CCCAGACCAC CCCTGTAGCA GGGTCTCAGT CCCTTTCCTC TGGGGCAGTG GCAACGACCA CGTCCTCTGC TGGTGATCAC AATGGCATGC TCTCTGTCCC TGCCCCTGGG GTGCCCCACT CTGATGTGGG GGAGCCACCT GCCTCTACTG GGGCCCAGCA GCCTTCTGCA TTAGCCGCCC TGCAGCCAGC AAAGGAGCGG CGGAGTTCCT CCTCGTCGTC GTCGTCCTCT AGCTCCTCCT CTTCTTCATC ATCGTCGTCG TCGTCCTCCT CCTCCTCTGG CTCCAGTTCT AGTGACTCAG AGGGCTCTAG CCTTCCTGTG CAACCTGAGG TGGCACTGAA GAGGGTCCCC AGCCCCACCC CAGCCCCAAA GGAGGCTGTT CGAGAGGGAC GTCCTCCGGA GCCAACCCCA GCCAAACGGA AGAGGCGCTC TAGCAGTTCC AGTTCCAGCT CCTCCTCTTC ATCTTCCTCC TCCTCCTCCT CCTCCTCTTC TTCCTCCTCC TCTTCCTCTT CTTCTTCTTC CTCCTCATCT TCCTCCTCCT CGTCGTCTTC CTCCCCTTCC CCTGCTAAGC CTGGCCCTCA GGCCTTGCCC AAACCTGCAA GCCCCAAGAA GCCACCCCCT GGCGAGCGGA GGTCCCGCAG CCCCCGGAAG CCAATAGACT CCCTCAGGGA CTCTCGGTCC CTCAGCTACT CGCCTGTGGA GCGTCGCCGT CCCTCGCCCC AGCCCTCACC ACGGGACCAG CAGAGCAGCA GCAGTGAGCG GGGTTCCCGG AGAGGCCAGC GTGGGGACAG CCGCTCCCCC AGCCACAAGC GCAGGAGGGA GACACCTAGC CCTCGGCCCA TGAGACACCG CTCCTCCAGG TCTCCAGGAT CCGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTA GCGGCCGCGA AGGATCTGCG ATCGCTCCGG TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT CGGCAATTGA ACGGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGCTGAAGC TTCGAGGGGC TCGCATCTCT CCTTCACGCG CCCGCCGCCC TACCTGAGGC CGCCATCCAC GCCGGTTGAG TCGCGTTCTG CCGCCTCCCG CCTGTGGTGC CTCCTGAACT GCGTCCGCCG TCTAGGTAAG TTTAAAGCTC AGGTCGAGAC CGGGCCTTTG TCCGGCGCTC CCTTGGAGCC TACCTAGACT CAGCCGGCTC TCCACGCTTT GCCTGACCCT GCTTGCTCAA CTCTACGTCT TTGTTTCGTT TTCTGTTCTG CGCCGTTACA GATCCAAGCT GTGACCGGCG CCTACGCTAG ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC CCGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT CGACCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA GTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAAATAA GATCTGCTTT TTGCTTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTAGTAGT TCATGTCATC TTATTATTCA GTATTTATAA CTTGCAAAGA AATGAATATC AGAGAGTGAG AGGAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGCTCTA GCTATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGACTTTTGC AGAGACGGCC CAAATTCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGCCAAGC TG
