pCMV-Adamts12(rat)-3×FLAG-Neo
$113.00
In stock
SKU
PL43514
NM_001106420.4
100μg liquid plasmid(Instantly):$134
| name | pCMV-Adamts12(rat)-3×FLAG-Neo | another name | apCMV-Adamts12(rat)-3×FLAG-Neo |
| Insert Size (bp) | 4800bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Rat | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCGCCACC ATGCCATGTG CTCAGGGGAA CTGGATGGCA AAGCTCTCCA TGGTGGCCCA GCTTCTTAAC TTTGGGGCTT TTTGCCATGG GAGACAAGCT CAGCCCTGGC CGGTTCGCTT CCCAGACCCG AAGCAAGAAC ATTTTATCAA GTCCCTGCCA GAATACCACA TAGTGAGTCC TGTCCAAGTA GATGCCAGTG GCCACTTTCT GTCCTATGGC CTGCATCACC CTGTCACTGG CAGCAGGAAG AAGAGAGCTG CAGGTGGTTC TGGGGACCAG GTGTACTACA GGATTTCACA CGAAGAAAAA AACCTGTTTT TCAATCTGAC AGTCAACTGG GAATTTCTTT CCAATGGCTA TGTCGTGGAG AGGAGATATG GGAACCTTTC CCATGTGAAG ATGGCGGCTT CCTCAGGGCA GCCCTGCCAT CTCAGGGGCA CAGTTCTGCA ACAGGGCCCC ACAATCAGGA TGGGGACAGC AGCTCTTAGT GCCTGCCAAG GACTGACTGG ATTTTTCCAT CTACCACATG GAGACTTTTT CATTGAGCCT GTTAAAAAGC ATCCACTGAC CGAGGAAGGT TACCAGCCTC ATGTCATATA CAGGAGGCAG AGCTACAGAG TTCCAGAGAC AAAGGAGCCC ACCTGCGGAT TAAAGGACAG CCTTGATAAC TCTGTGAAGC AAGAGCTCCA GCGGGAGAAG TGGGAGAGGA AGAACTGGCC AAGCAGAAGC CTCTCCCGAC GCTCCATAAG CAAGGAGAGA TGGGTGGAGA CCCTCGTGGT AGCTGACACG AAGATGGTAG AGTACCATGG AAGTGAGAAC GTGGAGTCGT ACATCCTCAC TATCATGAAT ATGGTCACTG GGTTGTTTCA TAACCCAAGC ATTGGAAACG CAGTGCACAT TGTTGTGGTT CGGCTCATTC TACTTGAAGA AGAGGAGCAA GGATTGAAAA TAGTTCACCA TGCAGAGAAG ACACTGTCCA GCTTCTGCAA GTGGCAGAAG AGCATCAATC CCAAGAGTGA CCTCAACCCT GTCCATCATG ATGTGGCTGT CCTTATCACC AGAAAGGACA TCTGTGCTGG TGTCAATCGC CCTTGTGAGA CTCTGGGGCT GTCCCAGCTG TCAGGAATGT GCCAGCCTCA CAGGAGCTGT AACATCAACG AAGACTCCGG TCTCCCCCTG GCTTTCACCA TTGCGCATGA ACTTGGGCAC AGCTTCGGCA TCCAACATGA TGGGAAGGAA AATGACTGTG AGCCCGTGGG CAGACACCCA TACATCATGT CCCAACAAAT TCAGTATGAC CCAACTCCAC TGACATGGTC GAAATGCAGC AAGGAGTACA TCACTCGCTT CTTGGACCGA GGCAGGGGGT TCTGTCTTGA TGACGTCCCC AGAAAGAAAG GCTTAAAGTC CAATGTCATT GCCCCTGGAG TGATTTATGA TGTTCATCAC CAGTGCCAGC TCCAATACGG CCCAAATGCT ACATTCTGCC AGGAAGTGGA AAATGTTTGC CAGACACTGT GGTGCTCAGT GAAAGGCTTC TGTCGCTCTA AGCTGGATGC TGCTGCAGAT GGGACACGTT GTGGTGAGAA GAAGTGGTGT ATGGCTGGAA AGTGTATCAC AGTGGGGAAG AAACCAGAGA GCATCCCTGG AGGCTGGGGA CGCTGGTCAC CCTGGTCACA CTGTTCGAGG ACATGTGGGG CAGGAGCTCA GAGCGCAGAG AGGCTCTGCA ACAACCCAGA ACCAAAGTTT GGAGGGAAAT ACTGCACCGG AGAAAGAAAA CGCTATCGCT TATGCAATGT CCACCCCTGC CGCTCAGACA CTCCAACTTT CCGACAGATG CAGTGCAGTG AATTTGACAC AGTCCCCTAC AAAAACCAAT TCTACCGCTG GTTCCCAGTG TTTAATCCAG CACATCCTTG TGAGCTGTAC TGCAGACCCA TAGATGAACA GTTTTCTGAG AGAATGCTGG AAGCCGTCAT TGATGGCACC CCGTGCTTTG AAGGTGGCAA CAGCAGAAAT GTCTGTATTA ATGGCATATG TAAGAGGGTT GGCTGTGACT ATGAGATTGA TTCCAATGCT ACAGAGGATC GCTGTGGCGT GTGCCTTGGA GATGGCTCTG CCTGCCAGAC TGTGAAGAAG GTGTTTAGAC AGAAGGAAGG ATCCGGTTAC ATTGACATCG GACTTATTCC CAAAGGTGCA AGGGATATAA GAGTGATGGA AATCAAGGCA GCCGGCAATT TCCTGGCCAT TAGGAGTGAA GATCCAGAAA AATATTACCT CAACGGAGGA TTTATCATCC AGTGGAATGG AAACTATAAA CTGGCAGGGA CCGTCTTCCA GTATGACAGG AAAGGAGATC TGGAGAGACT TATGGCTCCA GGCCCCACCA ACGAGTCAGT GTGGCTCCAG CTACTATTCC AGGTGACTAA CCCCGGTATC AAGTATGAGT ACACAGTCCG GAAAGATGGC CTTGACAATG ATGTGGAGAA GTTGCTGTAC TTCTGGCAAT TTGGCCGCTG GACAGAGTGC AGTGTAACAT GTGGAACAGG TATCCGCCGG CAGACTGCTC ATTGTGTCAA GAAGGGCCAT GGGATAGTGA AAACTACCTT CTGCAACCCA GAAACACAGC CCAGCGTGAG ACAGAAAAAG TGCTATGAAA AGGACTGTCC ACCCAGGTGG TGGGCCGGAG AGTGGGAAGC ATGTTCAATG ACATGTGGGC CGTACGGAGA AAAAAAGAGA ACAGTGCTGT GCATCCAGAC CATGGGCTCT GATGAGCAGG CTCTCCCAGC TACAGACTGC CAGCACCTGC TGAAGCCCAA GACACTGGTT TCCTGCAACA GAGACATTCT GTGTCCATCA GATTGGACAG TAGGCAACTG GAGTGAGTGC TCTGTTTCCT GTGGTGGTGG AGTGCGGATT CGCAGTGTCA CATGTGCCAA GAACCTTAAT GAACCCTGTG ACAAGACACG GAAACCCAAC AGTCGAGCTC TGTGTGGCCT CCAGCAGTGC CCATTCAGCC GTAGAGTTCT GAAACCCAAT AAAGATACAG TTCCCAGTGG GAAAAATCCA ACAACATCAG AACATGACCA CTTCAAGCCC ATCCCAGCAT CTACATCCAG GCCCACGCCA CTGTCCACAC CCACAGTGCC TGAGTCTGTG AGCACAAGTA CCCCCACAAT CAACAGCCTT GGCTCCACCA TAACATCCCA AGAAGAACCA GATGGGATAG GATGGCAGAA TAATTCAACC CAAGCTGAAG AGGACTCCCA CATCCCTACT TCTGTTGGAA GCACTTCCCA GACTCCCCTC ACTTCCTGGT CTTGGAGTAT GCAGCCTGAT GATGAAAACG TTTCCAGTTC AGCTATAGGT CCAACTTCAG AGAGTGACTT TTGGGCAACC ACAAGTGATT CTGGCTTATC ATCTAGCAAT GCTATGACTT GGCAGGTAAC TCCATTTTAT AGCACTGCGA CCACAGAACC GGAAGTGGAG ATACATAGTG GCTCAGGGGA AGACAGTGAC CAGCCTTTGA ACAAGGAGGA AAACAACTCT GTGCTATGGA ACAAGATCAG AGTACCTGAA CGTGATGCTC CCATGGAGAT GGATGCAGAA ATACCACTTG GACCTCCACC AACATCTTAT GTGACAGAAG AGTCCTCATG GCCTCCCTTC AGTACAATGA TGAAAAGCTC ACTACCTGCC TGGTCCTTCA AAAATGAGAC ACCCAGGGAT GAGGGGATGA TCACTGAAAA ATCAGGGAAT ATTCCACTCC CTCTTGGAGG AGACCACCAG ACAACATCCC CAGAGAAGTT AGGAAACAAT GACCAACTGG CATCAGCAAA TAGTACAAAT CCAACCCAAG GCTCTGGGCC TGTCCTGACT GAGGAAGATG CCTCCACTCT GATTGAGGAG GGATTTTTGC TAAATGCTTC CAATTACAAG CATCTCATGA AGGACCACAG CCCTGCACAC TGGATTGTTG GAAATTGGAG TAAATGCTCT ACCACCTGTG GGCTGGGGGC CTACTGGAGG AGTGTGGAGT GTAGCACCGG GATGAACGCT GACTGCGCCG CCATTCAGAG GCCAGACCCT GCTAAGAAGT GCCACCTGCG CCCCTGTGCT GGCTGGCGAG TGGGAAACTG GAGCAAGTGC TCTCGAAACT GCAGTGGAGG TTTCAAGATT CGTGAGGTTC AGTGCATGGA CGGCGTGGAC CATCACCGGA GCCTGAGGCC ATTTCACTGT CAGTTCCTGG CCGGCGTTCC TCCTCCGCTG AGTATGAGCT GTAATCTGGA GCCTTGCGAA GAGTGGAAAG TGGAACCCTG GAGCCAGTGT TCTAGGTCCT GTGGAGGCGG TGTTCAGGAG AGAGGAGTGT TTTGCCCAGG AGGTCTCTGC GACTGGACAA AAAGACCTGC ATCCACTGTG CCCTGCAACA GACACCTTTG CTGTCACTGG GCCACTGGGA ACTGGGAACT GTGCACCACT TCCTGTGGTG GTGGCTCTCA GAAGAGGACC GTTCATTGCA TCCCTTCAGA AAACAGTACA ACTGAAGATC AAGACCAGTG TTTCTGTGAC CACCAAGCCA GACCCCCAGA ATTCCAAAAC TGCAACCAGC AAGCCTGCCG GAAGAGTGCT GATTTAACTT GCACGAAGGA CAGACTTTCA ACCAGCTTCT GCCAGACACT AAAATCCATG AAGAAGTGCT CGGTGCCATC AGTACGAGTC CAGTGCTGTC TCTCGTGTCC ACAGACACAG AGCATCCACA CCCAGAGGCA AAGAAAACAG CAGATGCTCC AAAATCATGA CACGCTCCTC GAGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGT AGTGAGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTC
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