pADAM23(human)-miniP-Fluc
$70.00
In stock
SKU
PL43470
100μg liquid plasmid(Instantly):$91
| name | pADAM23(human)-miniP-Fluc | another name | apADAM23(human)-miniP-Fluc |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Element |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Fluc | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTAACTG GCCGGTACCA TTACAGCTTG GTAAAAGGGC TAAGGAATAG GGAAAGAATT TCATTTATGA TGAAAGTGTC ACCCTTGGTA AGGAAAATAC AAATAAATAA TTATGAAAAA TCAACAGAGG AACCACCTCG ACATCCCCAC TCTAGGGGTG TAGGGAGGAG AGCAAAGAAA TTCTATGGAG GAGGTAGCAT TTGAACAACT GGTAAAACAC AGGTATACGG AAATGGAGAG AAAAATGTTG AGAGAAATGG AGAGAAAAAG CTTGAGCTAG GTAGGATACG CAAATTCAGC TGATGTAACA ATGACCAAAA TACCAGAGGC TTAAAGAAGA GTGAAGATTT CCTCCCCATG ATGAGCAGTC CAGAGCTGGT AGGGAGGCCC TACACACCTG CTGGCTTCTT GCTGTGCAAT CCTTTGCACC AGCCTTCACC TCATGGAACG AATGAGCTGT TCCAGCTCCC ATCACCCTGT CTGCCTTCCA GGAATCAGGA GGGAAAGGAG GAGACACAGG CTCGAGGATA TCAAGATCTG GCCTCGGCGG CCAAGCTTAG ACACTAGAGG GTATATAATG GAAGCTCGAC TTCCAGCTTG GCAATCCGGT ACTGTTGGTA AAGCCACCAT GGAAGATGCC AAAAACATTA AGAAGGGCCC AGCGCCATTC TACCCACTCG AAGACGGGAC CGCCGGCGAG CAGCTGCACA AAGCCATGAA GCGCTACGCC CTGGTGCCCG GCACCATCGC CTTTACCGAC GCACATATCG AGGTGGACAT TACCTACGCC GAGTACTTCG AGATGAGCGT TCGGCTGGCA GAAGCTATGA AGCGCTATGG GCTGAATACA AACCATCGGA TCGTGGTGTG CAGCGAGAAT AGCTTGCAGT TCTTCATGCC CGTGTTGGGT GCCCTGTTCA TCGGTGTGGC TGTGGCCCCA GCTAACGACA TCTACAACGA GCGCGAGCTG CTGAACAGCA TGGGCATCAG CCAGCCCACC GTCGTATTCG TGAGCAAGAA AGGGCTGCAA AAGATCCTCA ACGTGCAAAA GAAGCTACCG ATCATACAAA AGATCATCAT CATGGATAGC AAGACCGACT ACCAGGGCTT CCAAAGCATG TACACCTTCG TGACTTCCCA TTTGCCACCC GGCTTCAACG AGTACGACTT CGTGCCCGAG AGCTTCGACC GGGACAAAAC CATCGCCCTG ATCATGAACA GTAGTGGCAG TACCGGATTG CCCAAGGGCG TAGCCCTACC GCACCGCACC GCTTGTGTCC GATTCAGTCA TGCCCGCGAC CCCATCTTCG GCAACCAGAT CATCCCCGAC ACCGCTATCC TCAGCGTGGT GCCATTTCAC CACGGCTTCG GCATGTTCAC CACGCTGGGC TACTTGATCT GCGGCTTTCG GGTCGTGCTC ATGTACCGCT TCGAGGAGGA GCTATTCTTG CGCAGCTTGC AAGACTATAA GATTCAATCT GCCCTGCTGG TGCCCACACT ATTTAGCTTC TTCGCTAAGA GCACTCTCAT CGACAAGTAC GACCTAAGCA ACTTGCACGA GATCGCCAGC GGCGGGGCGC CGCTCAGCAA GGAGGTAGGT GAGGCCGTGG CCAAACGCTT CCACCTACCA GGCATCCGCC AGGGCTACGG CCTGACAGAA ACAACCAGCG CCATTCTGAT CACCCCCGAA GGGGACGACA AGCCTGGCGC AGTAGGCAAG GTGGTGCCCT TCTTCGAGGC TAAGGTGGTG GACTTGGACA CCGGTAAGAC ACTGGGTGTG AACCAGCGCG GCGAGCTGTG CGTCCGTGGC CCCATGATCA TGAGCGGCTA CGTTAACAAC CCCGAGGCTA CAAACGCTCT CATCGACAAG GACGGCTGGC TGCACAGCGG CGACATCGCC TACTGGGACG AGGACGAGCA CTTCTTCATC GTGGACCGGC TGAAGAGCCT GATCAAATAC AAGGGCTACC AGGTAGCCCC AGCCGAACTG GAGAGCATCC TGCTGCAACA CCCCAACATC TTCGACGCCG GGGTCGCCGG CCTGCCCGAC GACGATGCCG GCGAGCTGCC CGCCGCAGTC GTCGTGCTGG AACACGGTAA AACCATGACC GAGAAGGAGA TCGTGGACTA TGTGGCCAGC CAGGTTACAA CCGCCAAGAA GCTGCGCGGT GGTGTTGTGT TCGTGGACGA GGTGCCTAAA GGACTGACCG GCAAGTTGGA CGCCCGCAAG ATCCGCGAGA TTCTCATTAA GGCCAAGAAG GGCGGCAAGA TCGCCGTGTA ATAATTCTAG AGTCGGGGCG GCCGGCCGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGATAAGGA TCCGTCGACC GATGCCCTTG AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC GTAGGACAGG TGCCGGCAGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGCGGCCGC AAATGCTAAA CCACTGCAGT GGTTACCAGT GCTTGATCAG TGAGGCACCG ATCTCAGCGA TCTGCCTATT TCGTTCGTCC ATAGTGGCCT GACTCCCCGT CGTGTAGATC ACTACGATTC GTGAGGGCTT ACCATCAGGC CCCAGCGCAG CAATGATGCC GCGAGAGCCG CGTTCACCGG CCCCCGATTT GTCAGCAATG AACCAGCCAG CAGGGAGGGC CGAGCGAAGA AGTGGTCCTG CTACTTTGTC CGCCTCCATC CAGTCTATGA GCTGCTGTCG TGATGCTAGA GTAAGAAGTT CGCCAGTGAG TAGTTTCCGA AGAGTTGTGG CCATTGCTAC TGGCATCGTG GTATCACGCT CGTCGTTCGG TATGGCTTCG TTCAACTCTG GTTCCCAGCG GTCAAGCCGG GTCACATGAT CACCCATATT ATGAAGAAAT GCAGTCAGCT CCTTAGGGCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC GGTGTTGTCG CTCATGGTAA TGGCAGCACT ACACAATTCT CTTACCGTCA TGCCATCCGT AAGATGCTTT TCCGTGACCG GCGAGTACTC AACCAAGTCG TTTTGTGAGT AGTGTATACG GCGACCAAGC TGCTCTTGCC CGGCGTCTAT ACGGGACAAC ACCGCGCCAC ATAGCAGTAC TTTGAAAGTG CTCATCATCG GGAATCGTTC TTCGGGGCGG AAAGACTCAA GGATCTTGCC GCTATTGAGA TCCAGTTCGA TATAGCCCAC TCTTGCACCC AGTTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCGG GGTGTGCAAA AACAGGCAAG CAAAATGCCG CAAAGAAGGG AATGAGTGCG ACACGAAAAT GTTGGATGCT CATACTCGTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTACTAGT ACGTCTCTCA AGGATAAGTA AGTAATATTA AGGTACGGGA GGTATTGGAC AGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGAATC GATAGTACTA ACATACGCTC TCCATCAAAA CAAAACGAAA CAAAACAAAC TAGCAAAATA GGCTGTCCCC AGTGCAAGTG CAGGTGCCAG AACATTTCTC T
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