NM_198834.3;ACAC; Acac1; ACACAD; ACACalpha; ACC; ACC1;ACCA; ACCalpha; hACC1
100μg liquid plasmid(Instantly):$178
| name | pWPXL-ACACA(human) | another name | apWPXL-ACACA(human) |
| Insert Size (bp) | 7152bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTGGAAGGGC TAATTCACTC CCAAAGAAGA CAAGATATCC TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA TTAGCAGAAC TACACACCAG GGCCAGGGGT CAGATATCCA CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC CAGATAAGGT AGAAGAGGCC AATAAAGGAG AGAACACCAG CTTGTTACAC CCTGTGAGCC TGCATGGGAT GGATGACCCG GAGAGAGAAG TGTTAGAGTG GAGGTTTGAC AGCCGCCTAG CATTTCATCA CGTGGCCCGA GAGCTGCATC CGGAGTACTT CAAGAACTGC TGATATCGAG CTTGCTACAA GGGACTTTCC GCTGGGGACT TTCCAGGGAG GCGTGGCCTG GGCGGGACTG GGGAGTGGCG AGCCCTCAGA TCCTGCATAT AAGCAGCTGC TTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT GGAGAAGTGA ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA GTAGCACCCA CCAAGGCAAA GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG ACGCTGACGG TACAGGCCAG ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG CTGAGGGCTA TTGAGGCGCA ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTAAT ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG AATTATTGGA ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC TGTGGTATAT AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT TTGCTGTACT TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCAGA CCCACCTCCC AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA GAAGGTGGAG AGAGAGACAG AGACAGATCC ATTCGATTAG TGAACGGATC TCGACGGTAT CGATGTCGAC GATAAGCTTT GCAAAGATGG ATAAAGTTTT AAACAGAGAG GAATCTTTGC AGCTAATGGA CCTTCTAGGT CTTGAAAGGA GTGGGAATTG GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGAACC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG GTAAGTGCCG TGTGTGGTTC CCGCGGGCCT GGCCTCTTTA CGGGTTATGG CCCTTGCGTG CCTTGAATTA CTTCCACTGG CTGCAGTACG TGATTCTTGA TCCCGAGCTT CGGGTTGGAA GTGGGTGGGA GAGTTCGAGG CCTTGCGCTT AAGGAGCCCC TTCGCCTCGT GCTTGAGTTG AGGCCTGGCC TGGGCGCTGG GGCCGCCGCG TGCGAATCTG GTGGCACCTT CGCGCCTGTC TCGCTGCTTT CGATAAGTCT CTAGCCATTT AAAATTTTTG ATGACCTGCT GCGACGCTTT TTTTCTGGCA AGATAGTCTT GTAAATGCGG GCCAAGATCT GCACACTGGT ATTTCGGTTT TTGGGGCCGC GGGCGGCGAC GGGGCCCGTG CGTCCCAGCG CACATGTTCG GCGAGGCGGG GCCTGCGAGC GCGGCCACCG AGAATCGGAC GGGGGTAGTC TCAAGCTGGC CGGCCTGCTC TGGTGCCTGG CCTCGCGCCG CCGTGTATCG CCCCGCCCTG GGCGGCAAGG CTGGCCCGGT CGGCACCAGT TGCGTGAGCG GAAAGATGGC CGCTTCCCGG CCCTGCTGCA GGGAGCTCAA AATGGAGGAC GCGGCGCTCG GGAGAGCGGG CGGGTGAGTC ACCCACACAA AGGAAAAGGG CCTTTCCGTC CTCAGCCGTC GCTTCATGTG ACTCCACGGA GTACCGGGCG CCGTCCAGGC ACCTCGATTA GTTCTCGAGC TTTTGGAGTA CGTCGTCTTT AGGTTGGGGG GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA AGTTAGGCCA GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGA ATTTCGACAT TTAAATTTAA TTAATCTCGA CGGTATCGGT TAACTTTTAA AAGAAAAGGG GGGATTGGGG GGTACAGTGC AGGGGAAAGA ATAGTAGACA TAATAGCAAC AGACATACAA ACTAAAGAAT TACAAAAACA AATTACAAAA ATTCAAAATT TTCCGATCAC GAGACTAGCC TCGAGGTTTA AACTACGGGA TCCAGGCCTA AGCTTACGCG TCCTAGCGCT ACCGGTCGCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TCCGGACTCA GATCTCGACT AGCTAGTAGC ATGTGGTGGT CTACTCTGAT GTCAATCTTG AGGGCTAGGT CTTTTTGGAA GTGGATATCT ACTCAGACAG TAAGAATTAT AAGAGCTGTA AGAGCTCATT TTGGAGGAAT AATGGATGAA CCATCTCCCT TGGCCCAACC TCTGGAGCTG AACCAGCACT CTCGATTCAT AATAGGTTCT GTGTCTGAAG ATAACTCAGA GGATGAGATC AGCAACCTGG TGAAGTTGGA CCTACTGGAG GAGAAGGAGG GCTCCTTGTC ACCTGCTTCT GTTGGCTCAG ATACACTCTC TGATTTGGGG ATCTCTAGCC TACAGGATGG CTTGGCCTTG CACATAAGGT CCAGCATGTC TGGCTTGCAC CTAGTAAAGC AGGGCCGAGA CAGAAAGAAA ATAGATTCTC AACGAGATTT CACTGTGGCT TCTCCAGCAG AATTTGTTAC TCGCTTTGGG GGAAATAAAG TGATTGAGAA GGTTCTTATT GCTAACAATG GCATTGCAGC AGTGAAATGC ATGCGGTCTA TCCGTAGGTG GTCTTATGAA ATGTTTCGAA ATGAACGTGC AATTAGATTC GTTGTCATGG TCACACCTGA AGACCTTAAA GCCAATGCAG AATACATTAA GATGGCAGAT CACTATGTGC CAGTGCCTGG AGGACCAAAC AACAACAACT ATGCAAATGT GGAATTAATT CTTGATATTG CTAAAAGGAT CCCAGTACAA GCAGTGTGGG CTGGCTGGGG TCATGCTTCT GAGAATCCCA AACTACCGGA ACTTCTCTTG AAAAATGGCA TTGCCTTCAT GGGTCCTCCA AGCCAGGCCA TGTGGGCTTT AGGGGATAAG ATTGCATCTT CCATAGTGGC TCAAACTGCA GGTATCCCAA CTCTTCCCTG GAGCGGCAGT GGTCTTCGTG TGGACTGGCA GGAAAATGAT TTTTCAAAAC GTATCTTAAA TGTTCCCCAG GAGCTATATG AAAAAGGTTA TGTGAAAGAT GTGGATGATG GGCTACAGGC AGCTGAGGAA GTTGGATATC CAGTAATGAT CAAGGCCTCA GAGGGAGGAG GAGGGAAGGG AATTAGAAAA GTCAACAATG CAGATGACTT CCCTAATCTC TTCAGACAGG TTCAAGCTGA AGTTCCTGGA TCTCCCATAT TTGTGATGAG ACTAGCCAAA CAATCTCGTC ATCTGGAGGT GCAGATCTTA GCGGACCAAT ATGGCAATGC TATCTCTTTG TTTGGTCGTG ATTGCTCTGT ACAACGCAGG CATCAGAAGA TTATTGAAGA AGCACCTGCT ACTATTGCTA CTCCAGCAGT ATTTGAACAC ATGGAACAGT GTGCGGTGAA ACTTGCCAAA ATGGTGGGTT ATGTGAGTGC TGGGACTGTG GAATACCTGT ACAGCCAGGA TGGCAGCTTC TACTTTCTGG AATTGAATCC TCGGCTGCAG GTAGAGCACC CTTGTACAGA GATGGTGGCT GATGTCAATC TCCCTGCAGC ACAGCTCCAG ATTGCCATGG GGATTCCTCT ATATAGAATC AAGGATATCC GTATGATGTA TGGGGTATCT CCCTGGGGTG ATTCTCCCAT TGATTTTGAA GATTCTGCAC ACGTTCCTTG TCCAAGGGGC CATGTTATTG CTGCTCGGAT CACTAGTGAA AATCCAGATG AGGGTTTTAA GCCCAGCTCA GGAACAGTTC AGGAGCTAAA TTTCCGCAGC AATAAGAATG TTTGGGGATA TTTCAGTGTT GCTGCTGCAG GGGGACTTCA TGAATTTGCT GATTCTCAGT TTGGTCACTG CTTTTCTTGG GGAGAAAACA GAGAAGAGGC AATTTCAAAC ATGGTGGTGG CTTTGAAGGA GCTGTCTATT CGGGGTGACT TTCGAACTAC AGTTGAATAC CTGATCAAAT TGTTAGAGAC TGAAAGCTTT CAGATGAACA GAATTGATAC TGGCTGGCTG GACAGACTGA TAGCAGAAAA AGTACAGGCT GAGCGACCTG ACACCATGTT GGGGGTTGTG TGTGGTGCCC TCCACGTGGC AGATGTGAGC CTGCGGAATA GCGTCTCTAA CTTCCTTCAC TCCTTAGAAA GGGGTCAAGT CCTTCCTGCT CATACACTTC TGAATACAGT AGATGTTGAA CTTATCTATG AGGGAGTCAA GTATGTACTT AAGGTGACTC GACAGTCCCC CAACTCCTAT GTGGTGATCA TGAATGGCTC ATGTGTAGAA GTAGATGTAC ATCGGCTGAG TGACGGTGGA CTGCTCTTGT CCTATGATGG CAGCAGTTAT ACTACGTATA TGAAAGAGGA AGTGGATAGA TATCGCATCA CAATTGGCAA TAAAACCTGT GTGTTTGAGA AGGAAAATGA CCCATCGGTG ATGCGCTCAC CTTCTGCTGG GAAGTTAATC CAGTACATTG TAGAAGATGG AGGTCATGTG TTTGCCGGCC AGTGCTATGC TGAGATTGAG GTAATGAAGA TGGTAATGAC CTTAACAGCT GTGGAGTCTG GCTGTATCCA TTACGTCAAG CGACCTGGAG CAGCTCTTGA CCCTGGCTGT GTACTAGCCA AAATGCAACT GGACAACCCC AGCAAGGTTC AGCAGGCTGA ACTTCACACA GGTAGTCTGC CACGGATCCA GAGCACGGCA CTCAGAGGCG AGAAACTCCA TCGAGTGTTC CATTATGTCC TGGATAATCT GGTCAATGTA ATGAATGGAT ACTGCCTTCC AGATCCTTTC TTTAGCAGCA AGGTAAAAGA CTGGGTAGAG CGATTGATGA AAACCCTCAG AGATCCCTCC CTGCCTCTCC TAGAATTGCA AGATATTATG ACCAGTGTGT CTGGCCGCAT TCCCCCCAAT GTGGAGAAGT CTATCAAGAA GGAAATGGCT CAGTATGCTA GCAACATCAC ATCAGTCCTC TGTCAGTTTC CCAGCCAGCA GATTGCAAAC ATCCTAGATA GCCATGCAGC TACATTGAAC CGGAAATCTG AACGGGAAGT CTTCTTTATG AATACTCAGA GCATTGTTCA GCTGGTACAG AGGTACCGAA GTGGCATCCG AGGCCACATG AAGGCTGTGG TGATGGATCT GCTCCGGCAG TACCTGCGAG TAGAGACACA ATTCCAGAAT GGTCACTATG ACAAATGTGT ATTCGCCCTC CGAGAAGAGA ATAAAAGTGA CATGAACACT GTACTGAACT ACATCTTCTC TCACGCTCAA GTCACCAAGA AGAATCTTCT GGTCACAATG CTTATTGATC AGTTGTGTGG CCGGGACCCT ACTCTCACTG ATGAGCTGCT GAATATTCTC ACAGAGCTAA CTCAACTCAG TAAGACCACC AATGCCAAAG TAGCACTTCG AGCACGCCAG GTTCTTATTG CCTCCCATTT GCCATCATAT GAGCTTCGCC ATAACCAAGT AGAGTCTATC TTCCTATCAG CTATTGACAT GTATGGACAT CAATTTTGCA TTGAGAACCT GCAGAAACTC ATCCTATCAG AAACATCTAT TTTTGATGTC CTACCAAACT TCTTCTATCA CAGCAACCAA GTAGTGAGGA TGGCAGCTCT GGAGGTGTAT GTTCGAAGGG CTTATATTGC CTATGAACTT AACAGCGTAC AACACCGCCA GCTTAAGGAC AACACCTGTG TGGTGGAATT CCAGTTCATG CTGCCCACAT CTCATCCAAA CAGAGGGAAC ATCCCTACGC TAAACAGAAT GTCCTTCTCC TCCAACCTCA ACCACTATGG CATGACCCAT GTAGCTAGTG TCAGCGATGT ACTGTTGGAC AACTCATTCA CTCCACCTTG TCAGCGGATG GGCGGAATGG TCTCTTTTCG GACTTTTGAA GATTTTGTCA GGATCTTTGA TGAAGTGATG GGCTGCTTCT CTGACTCCCC ACCCCAGAGT CCCACATTCC CTGAGGCAGG TCACACGTCT CTTTATGATG AGGATAAGGT TCCCAGGGAT GAACCAATTC ACATTCTCAA TGTGGCTATC AAGACTGACT GTGATATTGA GGATGACAGG CTGGCAGCTA TGTTCAGAGA ATTTACCCAG CAAAATAAAG CTACCCTGGT TGACCATGGG ATCCGGCGCC TTACTTTCCT GGTTGCACAA AAGGATTTCA GAAAGCAGGT CAACTATGAG GTGGATCGGA GATTTCATAG AGAATTCCCT AAATTTTTTA CATTCCGAGC AAGGGATAAG TTTGAGGAGG ATCGTATCTA TCGTCATCTG GAGCCTGCTC TGGCTTTCCA GTTAGAGCTG AACCGGATGA GAAATTTTGA CCTCACTGCC ATTCCATGTG CTAATCACAA GATGCACCTG TATCTCGGGG CAGCCAAGGT GGAAGTGGGC ACAGAAGTGA CAGACTACAG GTTCTTTGTT CGTGCAATCA TCAGGCATTC TGATCTGGTC ACCAAGGAAG CTTCTTTTGA ATATCTGCAA AATGAAGGGG AGCGGCTACT CCTGGAAGCC ATGGATGAGT TGGAAGTTGC TTTTAACAAT ACAAATGTCC GCACTGACTG TAACCACATC TTCCTCAACT TTGTGCCCAC GGTTATCATG GACCCATCAA AGATTGAGGA ATCCGTGCGG AGCATGGTAA TGCGGTATGG AAGTCGCCTG TGGAAATTGC GCGTCCTCCA GGCAGAACTG AAAATCAACA TTCGCCTGAC GCCAACTGGA AAAGCAATTC CCATCCGCCT CTTCCTGACA AACGAGTCTG GCTATTACTT GGATATCAGC CTATACAAGG AAGTGACTGA CTCCAGGACA GCACAGATCA TGTTTCAGGC ATATGGAGAC AAACAGGGAC CACTGCATGG AATGTTAATC AATACTCCAT ATGTGACCAA AGACCTGCTG CAATCAAAGA GGTTCCAGGC ACAATCCTTA GGGACAACAT ACATATATGA TATCCCAGAG ATGTTTCGGC AGTCCCTGAT CAAACTCTGG GAGTCTATGT CCACTCAAGC ATTTCTTCCA TCTCCCCCTC TGCCTTCTGA CATGCTGACT TACACTGAAC TGGTACTGGA TGATCAAGGT CAGCTGGTCC ACATGAACAG GCTTCCAGGA GGAAATGAGA TTGGCATGGT AGCTTGGAAA ATGACCTTTA AAAGTCCTGA ATATCCAGAA GGCCGAGATA TCATTGTTAT TGGCAATGAC ATCACATACC GAATTGGGTC CTTTGGGCCT CAAGAGGATT TGTTATTTCT CAGAGCTTCC GAACTTGCTA GGGCAGAAGG TATTCCACGC ATCTATGTAT CAGCCAACAG TGGAGCAAGA ATCGGACTGG CAGAAGAAAT TCGCCATATG TTTCATGTGG CCTGGGTAGA TCCTGAGGAT CCTTACAAGG GATACAGGTA TTTATATCTG ACTCCTCAAG ATTATAAGAG AGTCAGTGCT CTCAACTCTG TCCATTGTGA ACACGTGGAA GATGAAGGAG AATCCAGGTA CAAGATAACA GATATTATTG GGAAAGAAGA GGGAATTGGA CCCGAGAACC TTCGAGGTTC TGGAATGATT GCTGGAGAAT CCTCATTGGC CTATAATGAG ATCATTACCA TCAGCCTGGT GACGTGCCGG GCCATTGGGA TTGGGGCTTA CCTTGTCCGG CTGGGACAGA GAACCATCCA GGTTGAGAAT TCTCACTTAA TTCTAACAGG AGCTGGAGCC CTCAACAAAG TCCTCGGGCG GGAAGTGTAC ACCTCCAATA ACCAGCTGGG GGGCATCCAG ATTATGCACA ACAATGGGGT GACCCACTGC ACTGTGTGTG ATGACTTTGA AGGGGTTTTC ACTGTCCTGC ACTGGCTGTC TTACATGCCC AAGAGCGTGC ACAGTTCAGT TCCTCTTCTG AACTCAAAGG ATCCTATAGA CAGAATCATC GAGTTTGTTC CCACAAAGAC CCCATACGAT CCTCGATGGA TGCTAGCAGG CCGTCCTCAC CCAACCCAAA AAGGTCAGTG GTTGAGTGGC TTTTTTGACT ATGGATCTTT CTCAGAGATT ATGCAGCCCT GGGCACAGAC TGTGGTGGTT GGTAGAGCCA GGCTAGGAGG AATACCTGTG GGAGTTGTTG CTGTAGAAAC CCGAACAGTA GAACTAAGTA TCCCAGCTGA TCCAGCAAAC CTGGATTCTG AAGCCAAGAT AATCCAGCAG GCTGGCCAGG TTTGGTTCCC AGATTCTGCG TTTAAGACGT ATCAGGCCAT CAAGGACTTC AACCGGGAAG GGCTGCCTCT GATGGTCTTT GCCAACTGGA GAGGCTTCTC TGGTGGAATG AAAGATATGT ACGACCAAGT GCTGAAGTTT GGTGCTTACA TTGTGGATGG CTTGAGGGAG TGCTGCCAGC CTGTGCTGGT TTACATTCCT CCCCAGGCTG AGCTGCGGGG TGGCTCCTGG GTGGTGATTG ACTCCTCCAT CAACCCCCGG CACATGGAGA TGTATGCTGA CCGAGAAAGC AGGGGATCTG TTCTGGAGCC AGAAGGGACA GTAGAAATCA AATTCCGCAG AAAGGATCTG GTGAAAACCA TGCGTCGGGT GGACCCAGTC TACATCCACT TGGCTGAGCG ATTGGGGACC CCAGAGCTAA GCACAGCTGA GCGGAAGGAG TTGGAGAACA AGTTGAAGGA GCGGGAGGAA TTCCTAATTC CCATTTACCA TCAGGTAGCC GTGCAGTTTG CTGACTTGCA CGACACACCA GGCCGGATGC AGGAGAAGGG TGTTATTAGC GATATCCTGG ATTGGAAAAC ATCCCGTACC TTCTTCTACT GGCGGCTGAG GCGTCTTCTG CTGGAGGACC TGGTCAAGAA GAAAATCCAC AATGCCAACC CTGAGCTGAC TGATGGCCAG ATTCAAGCCA TGTTAAGGCG CTGGTTTGTG GAAGTGGAAG GAACAGTGAA GGCTTATGTT TGGGACAATA ATAAGGATCT GGCGGAGTGG CTAGAGAAAC AGCTGACAGA GGAGGATGGT GTTCACTCGG TAATAGAGGA AAACATCAAA TGCATCAGCA GAGACTACGT CCTCAAGCAA ATCCGCAGCT TGGTCCAGGC CAATCCAGAG GTTGCCATGG ATTCCATCAT CCATATGACG CAGCACATAT CACCCACTCA GCGAGCAGAA GTCATACGGA TCCTCTCCAC AATGGATTCC CCTTCCACGT AGGAATTCCC GGGACTAGTC ATATGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG GTACGTATGG CCAGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATG GGATCAATTC ACCATGGGAA TAACTTCGTA TAGCATACAT TATACGAAGT TATGCTGCTT TTTGCTTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGCATCTA GAATTAATTC CGTGTATTCT ATAGTGTCAC CTAAATCGTA TGTGTATGAT ACATAAGGTT ATGTATTAAT TGTAGCCGCG TTCTAACGAC AATATGTACA AGCCTAATTG TGTAGCATCT GGCTTACTGA AGCAGACCCT ATCATCTCTC TCGTAAACTG CCGTCAGAGT CGGTTTGGTT GGACGAACCT TCTGAGTTTC TGGTAACGCC GTCCCGCACC CGGAAATGGT CAGCGAACCA ATCAGCAGGG TCATCGCTAG CCAGATCCTC TACGCCGGAC GCATCGTGGC CGGCATCACC GGCGCCACAG GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG GGAAGATCGG GCTCGCCACT TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC AGGCCCCGTG GCCGGGGGAC TGTTGGGCGC CATCTCCTTG CATGCACCAT TCCTTGCGGC GGCGGTGCTC AACGGCCTCA ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA GGGAGAGCGT CGAATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTG GACACAAGAC AGGCTTGCGA GATATGTTTG AGAATACCAC TTTATCCCGC GTCAGGGAGA GGCAGTGCGT AAAAAGACGC GGACTCATGT GAAATACTGG TTTTTAGTGC GCCAGATCTC TATAATCTCG CGCAACCTAT TTTCCCCTCG AACACTTTTT AAGCCGTAGA TAAACAGGCT GGGACACTTC ACATGAGCGA AAAATACATC GTCACCTGGG ACATGTTGCA GATCCATGCA CGTAAACTCG CAAGCCGACT GATGCCTTCT GAACAATGGA AAGGCATTAT TGCCGTAAGC CGTGGCGGTC TGTACCGGGT GCGTTACTGG CGCGTGAACT GGGTATTCGT CATGTCGATA CCGTTTGTAT TTCCAGCTAC GATCACGACA ACCAGCGCGA GCTTAAAGTG CTGAAACGCG CAGAAGGCGA TGGCGAAGGC TTCATCGTTA TTGATGACCT GGTGGATACC GGTGGTACTG CGGTTGCGAT TCGTGAAATG TATCCAAAAG CGCACTTTGT CACCATCTTC GCAAAACCGG CTGGTCGTCC GCTGGTTGAT GACTATGTTG TTGATATCCC GCAAGATACC TGGATTGAAC AGCCGTGGGA TATGGGCGTC GTATTCGTCC CGCCAATCTC CGGTCGCTAA TCTTTTCAAC GCCTGGCACT GCCGGGCGTT GTTCTTTTTA ACTTCAGGCG GGTTACAATA GTTTCCAGTA AGTATTCTGG AGGCTGCATC CATGACACAG GCAAACCTGA GCGAAACCCT GTTCAAACCC CGCTTTAAAC ATCCTGAAAC CTCGACGCTA GTCCGCCGCT TTAATCACGG CGCACAACCG CCTGTGCAGT CGGCCCTTGA TGGTAAAACC ATCCCTCACT GGTATCGCAT GATTAACCGT CTGATGTGGA TCTGGCGCGG CATTGACCCA CGCGAAATCC TCGACGTCCA GGCACGTATT GTGATGAGCG ATGCCGAACG TACCGACGAT GATTTATACG ATACGGTGAT TGGCTACCGT GGCGGCAACT GGATTTATGA GTGGGCCCCG GATCTTTGTG AAGGAACCTT ACTTCTGTGG TGTGACATAA TTGGACAAAC TACCTACAGA GATTTAAAGC TCTAAGGTAA ATATAAAATT TTTAAGTGTA TAATGTGTTA AACTACTGAT TCTAATTGTT TGTGTATTTT AGATTCCAAC CTATGGAACT GATGAATGGG AGCAGTGGTG GAATGCCTTT AATGAGGAAA ACCTGTTTTG CTCAGAAGAA ATGCCATCTA GTGATGATGA GGCTACTGCT GACTCTCAAC ATTCTACTCC TCCAAAAAAG AAGAGAAAGG TAGAAGACCC CAAGGACTTT CCTTCAGAAT TGCTAAGTTT TTTGAGTCAT GCTGTGTTTA GTAATAGAAC TCTTGCTTGC TTTGCTATTT ACACCACAAA GGAAAAAGCT GCACTGCTAT ACAAGAAAAT TATGGAAAAA TATTCTGTAA CCTTTATAAG TAGGCATAAC AGTTATAATC ATAACATACT GTTTTTTCTT ACTCCACACA GGCATAGAGT GTCTGCTATT AATAACTATG CTCAAAAATT GTGTACCTTT AGCTTTTTAA TTTGTAAAGG GGTTAATAAG GAATATTTGA TGTATAGTGC CTTGACTAGA GATCATAATC AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT CAACTGGATA ACTCAAGCTA ACCAAAATCA TCCCAAACTT CCCACCCCAT ACCCTATTAC CACTGCCAAT TACCTAGTGG TTTCATTTAC TCTAAACCTG TGATTCCTCT GAATTATTTT CATTTTAAAG AAATTGTATT TGTTAAATAT GTACTACAAA CTTAGTAG
