NM_000722.4;CACNA2; CACNL2A; CCHL2A; LINC01112;ncRNA-N3
100μg liquid plasmid(Instantly):$120
| name | PiggyBac-CMV-CACNA2D1(human)-Hyg | another name | aPiggyBac-CMV-CACNA2D1(human)-Hyg |
| Insert Size (bp) | 3276bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | EF1a-R：gagccagtacacgacatcac | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG ACACCTGGGG GGATACGGGG AAAAGGCCTC CAAGGCCTAC TAGTATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGATT CTAGAGCCAC CATGGCTGCT GGCTGCCTGC TGGCCTTGAC TCTGACACTT TTCCAATCTT TGCTCATCGG CCCCTCGTCG GAGGAGCCGT TCCCTTCGGC CGTCACTATC AAATCATGGG TGGATAAGAT GCAAGAAGAC CTTGTCACAC TGGCAAAAAC AGCAAGTGGA GTCAATCAGC TTGTTGATAT TTATGAGAAA TATCAAGATT TGTATACTGT GGAACCAAAT AATGCACGCC AGCTGGTAGA AATTGCAGCC AGGGATATTG AGAAACTTCT GAGCAACAGA TCTAAAGCCC TGGTGCGCCT GGCATTGGAA GCGGAGAAAG TTCAAGCAGC TCACCAGTGG AGAGAAGATT TTGCAAGCAA TGAAGTTGTC TACTACAATG CAAAGGATGA TCTCGATCCT GAGAAAAATG ACAGTGAGCC AGGCAGCCAG AGGATAAAAC CTGTTTTCAT TGAAGATGCT AATTTTGGAC GACAAATATC TTATCAGCAC GCAGCAGTCC ATATTCCTAC TGACATCTAT GAGGGCTCAA CAATTGTGTT AAATGAACTC AACTGGACAA GTGCCTTAGA TGAAGTTTTC AAAAAGAATC GCGAGGAAGA CCCTTCATTA TTGTGGCAGG TTTTTGGCAG TGCCACTGGC CTAGCTCGAT ATTATCCAGC TTCACCATGG GTTGATAATA GTAGAACTCC AAATAAGATT GACCTTTATG ATGTACGCAG AAGACCATGG TACATCCAAG GAGCTGCATC TCCTAAAGAC ATGCTTATTC TGGTGGATGT GAGTGGAAGT GTTAGTGGAT TGACACTTAA ACTGATCCGA ACATCTGTCT CCGAAATGTT AGAAACCCTC TCAGATGATG ATTTCGTGAA TGTAGCTTCA TTTAACAGCA ATGCTCAGGA TGTAAGCTGT TTTCAGCACC TTGTCCAAGC AAATGTAAGA AATAAAAAAG TGTTGAAAGA CGCGGTGAAT AATATCACAG CCAAAGGAAT TACAGATTAT AAGAAGGGCT TTAGTTTTGC TTTTGAACAG CTGCTTAATT ATAATGTTTC CAGAGCAAAC TGCAATAAGA TTATTATGCT ATTCACGGAT GGAGGAGAAG AGAGAGCCCA GGAGATATTT AACAAATACA ATAAAGATAA AAAAGTACGT GTATTCACGT TTTCAGTTGG TCAACACAAT TATGACAGAG GACCTATTCA GTGGATGGCC TGTGAAAACA AAGGTTATTA TTATGAAATT CCTTCCATTG GTGCAATAAG AATCAATACT CAGGAATATT TGGATGTTTT GGGAAGACCA ATGGTTTTAG CAGGAGACAA AGCTAAGCAA GTCCAATGGA CAAATGTGTA CCTGGATGCA TTGGAACTGG GACTTGTCAT TACTGGAACT CTTCCGGTCT TCAACATAAC CGGCCAATTT GAAAATAAGA CAAACTTAAA GAACCAGCTG ATTCTTGGTG TGATGGGAGT AGATGTGTCT TTGGAAGATA TTAAAAGACT GACACCACGT TTTACACTGT GCCCCAATGG GTATTACTTT GCAATCGATC CTAATGGTTA TGTTTTATTA CATCCAAATC TTCAGCCAAA GAACCCCAAA TCTCAGGAGC CAGTAACATT GGATTTCCTT GATGCAGAGT TAGAGAATGA TATTAAAGTG GAGATTCGAA ATAAGATGAT TGATGGGGAA AGTGGAGAAA AAACATTCAG AACTCTGGTT AAATCTCAAG ATGAGAGATA TATTGACAAA GGAAACAGGA CATACACATG GACACCTGTC AATGGCACAG ATTACAGTTT GGCCTTGGTA TTACCAACCT ACAGTTTTTA CTATATAAAA GCCAAACTAG AAGAGACAAT AACTCAGGCC AGATCAAAAA AGGGCAAAAT GAAGGATTCG GAAACCCTGA AGCCAGATAA TTTTGAAGAA TCTGGCTATA CATTCATAGC ACCAAGAGAT TACTGCAATG ACCTGAAAAT ATCGGATAAT AACACTGAAT TTCTTTTAAA TTTCAACGAG TTTATTGATA GAAAAACTCC AAACAACCCA TCATGTAACG CGGATTTGAT TAATAGAGTC TTGCTTGATG CAGGCTTTAC AAATGAACTT GTCCAAAATT ACTGGAGTAA GCAGAAAAAT ATCAAGGGAG TGAAAGCACG ATTTGTTGTG ACTGATGGTG GGATTACCAG AGTTTATCCC AAAGAGGCTG GAGAAAATTG GCAAGAAAAC CCAGAGACAT ATGAGGACAG CTTCTATAAA AGGAGCCTAG ATAATGATAA CTATGTTTTC ACTGCTCCCT ACTTTAACAA AAGTGGACCT GGTGCCTATG AATCGGGCAT TATGGTAAGC AAAGCTGTAG AAATATATAT TCAAGGGAAA CTTCTTAAAC CTGCAGTTGT TGGAATTAAA ATTGATGTAA ATTCCTGGAT AGAGAATTTC ACCAAAACCT CAATCAGAGA TCCGTGTGCT GGTCCAGTTT GTGACTGCAA AAGAAACAGT GACGTAATGG ATTGTGTGAT TCTGGATGAT GGTGGGTTTC TTCTGATGGC AAATCATGAT GATTATACTA ATCAGATTGG AAGATTTTTT GGAGAGATTG ATCCCAGCTT GATGAGACAC CTGGTTAATA TATCAGTTTA TGCTTTTAAC AAATCTTATG ATTATCAGTC AGTATGTGAG CCCGGTGCTG CACCAAAACA AGGAGCAGGA CATCGCTCAG CATATGTGCC ATCAGTAGCA GACATATTAC AAATTGGCTG GTGGGCCACT GCTGCTGCCT GGTCTATTCT ACAGCAGTTT CTCTTGAGTT TGACCTTTCC ACGACTCCTT GAGGCAGTTG AGATGGAGGA TGATGACTTC ACGGCCTCCC TGTCCAAGCA GAGCTGCATT ACTGAACAAA CCCAGTATTT CTTCGATAAC GACAGTAAAT CATTCAGTGG TGTATTAGAC TGTGGAAACT GTTCCAGAAT CTTTCATGGA GAAAAGCTTA TGAACACCAA CTTAATATTC ATAATGGTTG AGAGCAAAGG GACATGTCCA TGTGACACAC GACTGCTCAT ACAAGCGGAG CAGACTTCTG ACGGTCCAAA TCCTTGTGAC ATGGTTAAGC AACCCAGATA CCGAAAAGGG CCTGATGTCT GCTTTGATAA CAATGTCTTG GAGGATTATA CTGACTGTGG TGGTGTTTCT GGATTAAATC CCTCCCTGTG GTATATCATT GGAATCCAGT TTCTACTACT TTGGCTGGTA TCTGGCAGCA CACACCGCCT GTTATGAGCG GCCGCGAAGG ATCTGCGATC GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGAACG GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG CTGAAGCTTC GAGGGGCTCG CATCTCTCCT TCACGCGCCC GCCGCCCTAC CTGAGGCCGC CATCCACGCC GGTTGAGTCG CGTTCTGCCG CCTCCCGCCT GTGGTGCCTC CTGAACTGCG TCCGCCGTCT AGGTAAGTTT AAAGCTCAGG TCGAGACCGG GCCTTTGTCC GGCGCTCCCT TGGAGCCTAC CTAGACTCAG CCGGCTCTCC ACGCTTTGCC TGACCCTGCT TGCTCAACTC TACGTCTTTG TTTCGTTTTC TGTTCTGCGC CGTTACAGAT CCAAGCTGTG ACCGGCGCCT ACGTCGACAT GAAAAAGCCT GAACTCACCG CGACGTCTGT CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC CTGATGCAGC TCTCGGAGGG CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT GGATATGTCC TGCGGGTAAA TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTTAT CGGCACTTTG CATCGGCCGC GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAATTCAGC GAGAGCCTGA CCTATTGCAT CTCCCGCCGT GCACAGGGTG TCACGTTGCA AGACCTGCCT GAAACCGAAC TGCCCGCTGT TCTGCAGCCG GTCGCGGAGG CCATGGATGC GATCGCTGCG GCCGATCTTA GCCAGACGAG CGGGTTCGGC CCATTCGGAC CGCAAGGAAT CGGTCAATAC ACTACATGGC GTGATTTCAT ATGCGCGATT GCTGATCCCC ATGTGTATCA CTGGCAAACT GTGATGGACG ACACCGTCAG TGCGTCCGTC GCGCAGGCTC TCGATGAGCT GATGCTTTGG GCCGAGGACT GCCCCGAAGT CCGGCACCTC GTGCACGCGG ATTTCGGCTC CAACAATGTC CTGACGGACA ATGGCCGCAT AACAGCGGTC ATTGACTGGA GCGAGGCGAT GTTCGGGGAT TCCCAATACG AGGTCGCCAA CATCTTCTTC TGGAGGCCGT GGTTGGCTTG TATGGAGCAG CAGACGCGCT ACTTCGAGCG GAGGCATCCG GAGCTTGCAG GATCGCCGCG GCTCCGGGCG TATATGCTCC GCATTGGTCT TGACCAACTC TATCAGAGCT TGGTTGACGG CAATTTCGAT GATGCAGCTT GGGCGCAGGG TCGATGCGAC GCAATCGTCC GATCCGGAGC CGGGACTGTC GGGCGTACAC AAATCGCCCG CAGAAGCGCG GCCGTCTGGA CCGATGGCTG TGTAGAAGTA CTCGCCGATA GTGGAAACCG ACGCCCCAGC ACTCGTCCGA GGGCAAAGGA ATAGAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCAGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGAATTGAC TCAAATGATG TCAATTAGTC TATCAGAAGC TATCTGGTCT CCCTTCCGGG GGACAAGACA TCCCTGTTTA ATATTTAAAC AGCAGTGTTC CCAAACTGGG TTCTTATATC CCTTGCTCTG GTCAACCAGG TTGCAGGGTT TCCTGTCCTC ACAGGAACGA AGTCCCTAAA GAAACAGTGG CAGCCAGGTT TAGCCCCGGA ATTGACTGGA TTCCTTTTTT AGGGCCCATT GGTATGGCTT TTTCCCCGTA TCCCCCCAGG TGTCTGCAGG CTCAAAGAGC AGCGAGAAGC GTTCAGAGGA AAGCGATCCC GTGCCACCTT CCCCGTGCCC GGGCTGTCCC CGCACGCTGC CGGCTCGGGG ATGCGGGGGG AGCGCCGGAC CGGAGCGGAG CCCCGGGCGG CTCGCTGCTG CCCCCTAGCG GGGGAGGGAC GTAATTACAT CCCTGGGGGC TTTGGGGGGG GGCTGTCCCT GATATCTATA ACAAGAAAAT ATATATATAA TAAGTTATCA CGTAAGTAGA ACATGAAATA ACAATATAAT TATCGTATGA GTTAAATCTT AAAAGTCACG TAAAAGATAA TCATGCGTCA TTTTGACTCA CGCGGTCGTT ATAGTTCAAA ATCAGTGACA CTTACCGCAT TGACAAGCAC GCCTCACGGG AGCTCCAAGC GGCGACTGAG ATGTCCTAAA TGCACAGCGA CGGATTCGCG CTATTTAGAA AGAGAGAGCA ATATTTCAAG AATGCATGCG TCAATTTTAC GCAGACTATC TTTCTAGGGT TAATCTAGCT GCATCAGGAT CATATCGTCG GGTCTTTTTT CCGGCTCAGT CATCGCCCAA GCTGGCGCTA TCTGGGCATC GGGGAGGAAG AAGCCCGTGC CTTTTCCCGC GAGGTTGAAG CGGCATGGAA AGAGTTTGCC GAGGATGACT GCTGCTGCAT TGACGTTGAG CGAAAACGCA CGTTTACCAT GATGATTCGG GAAGGTGTGG CCATGCACGC CTTTAACGGT GAACTGTTCG TTCAGGCCAC CTGGGATACC AGTTCGTCGC GGCTTTTCCG GACACAGTTC CGGATGGTCA GCCCGAAGCG CATCAGCAAC CCGAACAATA CCGGCGACAG CCGGAACTGC CGTGCCGGTG TGCAGATTAA TGACAGCGGT GCGGCGCTGG GATATTACGT CAGCGAGGAC GGGTATCCTG GCTGGATGCC GCAGAAATGG ACATGGATAC CCCGTGAGTT ACCCGGCGGG CGCGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA T
