NM_181672.3;HINCUT-1; HRNT1; MRX106; O-GLCNAC; OGT1;XLID106
100μg liquid plasmid(Instantly):$120
| name | pCMV-OGT(human)-3×FLAG-Neo | another name | apCMV-OGT(human)-3×FLAG-Neo |
| Insert Size (bp) | 3141bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT TGGTACCGAG CTCGGATCCG CCACCATGGC GTCTTCCGTG GGCAACGTGG CCGACAGCAC AGAACCAACG AAACGTATGC TTTCCTTCCA AGGGTTAGCT GAGTTGGCAC ATCGAGAATA TCAGGCAGGA GATTTTGAGG CAGCTGAGAG ACACTGCATG CAGCTCTGGA GACAAGAGCC AGACAATACT GGTGTGCTTT TATTACTTTC ATCTATACAC TTCCAGTGTC GAAGGCTGGA CAGATCTGCT CACTTTAGCA CTCTGGCAAT TAAACAGAAC CCCCTTCTGG CAGAAGCTTA TTCGAATTTG GGGAATGTGT ACAAGGAAAG AGGGCAGTTG CAGGAGGCAA TTGAGCATTA TCGACATGCA TTGCGTCTCA AACCTGATTT CATCGATGGT TATATTAACC TGGCAGCCGC CTTGGTAGCA GCGGGTGACA TGGAAGGGGC AGTACAAGCT TACGTCTCTG CTCTTCAGTA CAATCCTGAT TTGTACTGTG TTCGCAGTGA CCTGGGGAAC CTGCTCAAAG CCCTGGGTCG CTTGGAAGAA GCCAAGGCAT GTTATTTGAA AGCAATTGAG ACGCAACCGA ACTTTGCAGT AGCTTGGAGT AATCTTGGCT GTGTTTTCAA TGCACAAGGG GAAATTTGGC TTGCAATTCA TCACTTTGAA AAGGCTGTCA CCCTTGACCC AAACTTTCTG GATGCTTATA TCAATTTAGG AAATGTCTTG AAAGAGGCAC GCATTTTTGA CAGAGCTGTG GCAGCTTATC TTCGTGCCCT AAGTTTGAGT CCAAATCACG CAGTGGTGCA CGGCAACCTG GCTTGTGTAT ACTATGAGCA AGGCCTGATA GATCTGGCAA TAGACACCTA CAGGCGGGCT ATCGAACTAC AACCACATTT CCCTGATGCT TACTGCAACC TAGCCAATGC TCTCAAAGAG AAGGGCAGTG TTGCTGAAGC AGAAGATTGT TATAATACAG CTCTCCGTCT GTGTCCCACC CATGCAGACT CTCTGAATAA CCTAGCCAAT ATCAAACGAG AACAGGGAAA CATTGAAGAG GCAGTTCGCT TGTATCGTAA AGCATTAGAA GTCTTCCCAG AGTTTGCTGC TGCCCATTCA AATTTAGCAA GTGTACTGCA GCAGCAGGGA AAACTGCAGG AAGCTCTGAT GCATTATAAG GAGGCTATTC GAATCAGTCC TACCTTTGCT GATGCCTACT CTAATATGGG AAACACTCTA AAGGAGATGC AGGATGTTCA GGGAGCCTTG CAGTGTTATA CGCGTGCCAT CCAAATTAAT CCTGCATTTG CAGATGCACA TAGCAATCTG GCTTCCATTC ATAAGGATTC AGGGAATATT CCAGAAGCCA TAGCTTCTTA CCGCACGGCT CTGAAACTTA AGCCTGATTT TCCTGATGCT TATTGTAACT TGGCTCATTG CCTGCAGATT GTCTGTGATT GGACAGACTA TGATGAGCGA ATGAAGAAGT TGGTCAGTAT TGTGGCTGAC CAGTTAGAGA AGAATAGGTT GCCTTCTGTG CATCCTCATC ATAGTATGCT ATATCCTCTT TCTCATGGCT TCAGGAAGGC TATTGCTGAG AGGCACGGCA ACCTGTGCTT AGATAAGATT AATGTTCTTC ATAAACCACC ATATGAACAT CCAAAAGACT TGAAGCTCAG TGATGGTCGG CTGCGTGTAG GATATGTGAG TTCCGACTTT GGGAATCATC CTACTTCTCA CCTTATGCAG TCTATTCCAG GCATGCACAA TCCTGATAAA TTTGAGGTGT TCTGTTATGC CCTGAGCCCA GACGATGGCA CAAACTTCCG AGTGAAGGTG ATGGCAGAAG CCAATCATTT CATTGATCTT TCTCAGATTC CATGCAATGG AAAAGCAGCT GATCGCATCC ATCAGGATGG AATTCATATC CTTGTAAATA TGAATGGCTA TACTAAGGGC GCTCGAAATG AGCTTTTTGC TCTCAGGCCA GCTCCTATTC AGGCAATGTG GCTGGGATAC CCTGGGACGA GTGGTGCGCT TTTCATGGAT TATATTATCA CTGATCAGGA AACTTCGCCA GCTGAAGTTG CTGAGCAGTA TTCCGAGAAA TTGGCTTATA TGCCCCACAC TTTTTTTATT GGTGATCATG CTAATATGTT CCCTCACCTG AAGAAAAAAG CAGTCATCGA TTTTAAGTCC AATGGGCACA TTTATGACAA TCGGATAGTT CTGAATGGCA TCGACCTCAA AGCATTTCTT GATAGTCTAC CAGATGTGAA AATTGTCAAG ATGAAGTGTC CTGATGGAGG AGACAATGCA GATAGCAGTA ACACAGCTCT TAATATGCCT GTTATTCCTA TGAATACTAT TGCAGAAGCA GTTATTGAAA TGATTAACCG AGGACAGATT CAAATAACAA TTAATGGATT CAGTATTAGC AATGGACTGG CAACTACTCA GATCAACAAT AAGGCTGCAA CTGGAGAGGA GGTTCCCCGT ACCATTATTG TAACCACCCG TTCTCAGTAC GGGTTACCAG AAGATGCCAT CGTATACTGT AACTTTAATC AGTTGTATAA AATTGACCCT TCTACTTTGC AGATGTGGGC AAACATTCTG AAGCGTGTTC CCAATAGTGT ACTCTGGCTG TTGCGTTTTC CAGCAGTAGG AGAACCTAAT ATTCAACAGT ATGCACAAAA CATGGGCCTG CCCCAGAACC GTATCATTTT TTCACCTGTT GCTCCTAAAG AGGAACACGT CAGGAGAGGC CAGCTGGCTG ATGTCTGCTT GGACACTCCA CTCTGTAATG GGCACACCAC AGGGATGGAT GTCCTCTGGG CAGGGACCCC CATGGTGACT ATGCCAGGAG AGACTCTTGC TTCTCGAGTT GCAGCATCCC AGCTCACTTG CTTAGGTTGT CTTGAGCTTA TTGCTAAAAA CAGACAAGAA TATGAAGACA TAGCTGTGAA GCTGGGAACT GATCTAGAAT ACCTGAAGAA AGTTCGTGGC AAAGTCTGGA AGCAAAGAAT ATCTAGCCCT CTGTTCAACA CCAAACAATA CACAATGGAA CTAGAGCGGC TCTATCTACA GATGTGGGAG CATTATGCAG CTGGCAACAA ACCTGACCAC ATGATTAAGC CTGTTGAAGT CACTGAGTCA GCACTCGAGG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGTAGTG AGGGCCCGAT ATCTCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GAAACGGGGG AGGCTAACTG AAACACGGAA GGAGACAATA CCGGAAGGAA CCCGCGCTAT GACGGCAATA AAAAGACAGA ATAAAACGCA CGGGTGTTGG GTCGTTTGTT CATAAACGCG GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCAGA TCTGCGCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGC ATCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGGGGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCGA CGGATCGGGA GATCTCCCGA TCCCCTATGG TCGACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATCT GCTCCCTGCT TGTGTGTTGG AGGTCGCTGA GTAGTGCGCG AGCAAAATTT AAGCTACAAC AAGGCAAGGC TTGACCGACA ATTGCATGAA GAATCTGCTT AGG
