pPIK3C3(human)-mCherry-hsp86-BSD
$84.00
In stock
SKU
PL43196
NM_002647.3;hVps34; Vps34; VPS34
100μg liquid plasmid(Instantly):$105
| name | pPIK3C3(human)-mCherry-hsp86-BSD | another name | apPIK3C3(human)-mCherry-hsp86-BSD |
| Insert Size (bp) | 2661bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGGCCGCTAA CGTAACAGAC TTAGGAGGAG ATCTAGATCT TTCATATATA TAATATTTTA AAGGGGTAAA AAAAAAAATA TATATAATAA TAACAAATAA ATAAATAAAG GAAAACAATA CAATACAATA TAATATAATT AATTTTATGT ATATATTAAG AAATATCTTA AAAAAAAAAA AAAAAAATCT GTTTCATATA AAGAATATAT AATATTTATA AAACGATGTT ATTCTTATAA AAAAAAAAAA AGAAAAAAAA AAATAAAAAA TAAAAGGATA CCCCACCTAG TCAAAAATAA ATAATATTAT ATGTATTAAA ATAATATTAT GGGTAATTTA ATATTATATA TATATATATA TATATATATA ATTTAAGTTT TTGTAGATTA TATATAATAT ATAATATATT ATATATTATA TATATATATA TAAATATATA TTGAAGGTGT TTTAAAAAAA AAAAAAAAGC CCCGCGGAAA GGGGCCATTG GATATATATT TAGTATTCCA TAGTATATAG TTAAATATAA TAATATTATA GTAAATTTTT GCATGAATTT AAAGAAAAAA AAAAAAAAAA TAACAAAAAG GAGTATTATA TAAATATAAC AAAATAATAA ATACTATACC TATATATATA TATATATATA TATACATATA TATAAATATA TAAATATATA AATATATTTA TATAAAAATA ATATTTTATA TATAATATTA TATTTTTATA ATATCTTTTT TTTTTTAAAT ATCATCTTTG TTTCTTTCTT TCTTTCTTTC TTTCTTTCTT TTTTTTTTTT TTTGATTTAT ATATTATTTT ATTTTGTATT TACAAAATAT AATAAAATAA TTTCATGTTT AGCAAAAATT TTAAATAAAA AATAATAATA TAAAATAAAA AGCATCATTT AATATAAAAT AATAAAATTA AAATAAAATA AGATAATTAC CAAAAATAAT AAAAAAAATT AAAAAGTATT AAAAAATATA TATCATATAT AAATATATAA ATTTTAACAA CGTGAGAAAT TAAGAAAAAA ATTATATTTA TATAAATAAT ATAATATTTA ATATATATCT CATATAATAT TATATATATA TATATATATA TATATATATT TTTTAAAAAA AAAGAAAAAA TAAGTTTATT TTTACCTTTT AATAAAAACA TATTTTTAGA TATACATAAA AATTTTTATA TTATTTTATT GGTATATATA TATATATATA TATATATAAT AATATATATA TAATAAAATA TATAAATATT TACATAAATA TTTTACTTTA AAAAGAAATT TTCCTTTTTT ATTATATTTT TTTTCTTCCC ACATTTCGAA TAAACTCGAG CCACCATGGG GGAAGCAGAG AAGTTTCACT ACATCTATAG TTGTGACCTG GATATCAACG TCCAGCTTAA GATAGGAAGC TTGGAAGGGA AGAGAGAACA AAAGAGTTAT AAAGCTGTCC TGGAAGACCC AATGTTGAAG TTCTCAGGAC TATATCAAGA GACATGCTCT GATCTTTATG TTACTTGTCA AGTTTTTGCA GAAGGGAAGC CTTTGGCCTT GCCAGTGAGA ACATCCTACA AAGCATTTAG TACAAGATGG AACTGGAATG AATGGCTGAA ACTACCAGTA AAATACCCTG ACCTGCCCAG GAATGCCCAA GTGGCCCTCA CCATATGGGA TGTGTATGGT CCCGGAAAAG CAGTGCCTGT AGGAGGAACA ACGGTTTCGC TCTTTGGAAA ATACGGCATG TTTCGCCAAG GGATGCATGA CTTGAAAGTC TGGCCTAATG TAGAAGCAGA TGGATCAGAA CCCACAAAAA CTCCTGGCAG AACAAGTAGC ACTCTCTCAG AAGATCAGAT GAGCCGTCTT GCCAAGCTCA CCAAAGCTCA TCGACAAGGA CACATGGTGA AAGTAGATTG GCTGGATAGA TTGACATTTA GAGAAATAGA AATGATAAAT GAGAGTGAAA AACGAAGTTC TAATTTCATG TACCTGATGG TTGAATTTCG ATGTGTCAAG TGTGATGATA AGGAATATGG TATTGTTTAT TATGAAAAGG ACGGTGATGA ATCATCTCCA ATTTTAACAA GTTTTGAATT AGTGAAAGTT CCTGACCCCC AGATGTCTAT GGAGAATTTA GTTGAGAGCA AACACCACAA GCTTGCCCGG AGTTTAAGAA GTGGACCTTC TGACCACGAT CTGAAACCCA ATGCTGCCAC GAGAGATCAG TTAAATATTA TTGTGAGTTA TCCACCAACC AAGCAACTTA CATATGAAGA ACAAGATCTT GTTTGGAAGT TTAGATATTA TCTTACGAAT CAAGAAAAAG CCTTGACAAA ATTCTTGAAA TGTGTTAATT GGGATCTACC TCAAGAGGCC AAACAGGCCT TGGAACTTCT GGGAAAATGG AAGCCGATGG ATGTAGAGGA CTCCTTGGAG CTGTTATCCT CTCATTACAC CAACCCAACT GTGAGGCGTT ATGCTGTTGC CCGGTTGCGA CAGGCCGATG ATGAGGATTT GTTGATGTAC CTATTACAAT TGGTCCAGGC TCTCAAATAT GAAAATTTTG ATGATATAAA GAATGGATTG GAACCTACCA AGAAGGATAG TCAGAGTTCA GTGTCAGAAA ATGTGTCAAA TTCTGGAATA AATTCTGCAG AAATAGATAG CTCCCAAATT ATAACCAGCC CCCTTCCTTC AGTCTCTTCA CCTCCTCCTG CATCAAAAAC AAAAGAAGTT CCAGATGGCG AAAATCTGGA ACAAGATCTC TGTACCTTCT TGATATCGAG AGCCTGCAAA AACTCAACAC TGGCTAATTA TTTATACTGG TATGTGATAG TGGAATGTGA AGATCAAGAT ACTCAGCAGA GAGATCCAAA GACCCATGAG ATGTACTTGA ACGTAATGAG AAGATTCAGC CAAGCATTGT TGAAGGGTGA TAAGTCTGTC AGAGTTATGC GTTCTTTGCT GGCTGCACAA CAGACATTTG TAGATCGGTT GGTGCATCTA ATGAAGGCAG TACAACGCGA AAGTGGAAAT CGTAAGAAAA AGAATGAGAG ACTACAGGCA TTGCTTGGAG ATAATGAAAA GATGAATTTG TCAGATGTGG AACTTATCCC GTTGCCTTTA GAACCCCAAG TGAAAATTAG AGGAATAATT CCGGAAACAG CTACACTGTT TAAAAGTGCC CTTATGCCTG CACAGTTGTT TTTTAAGACG GAAGATGGAG GCAAATATCC AGTTATATTT AAGCATGGAG ATGATTTACG TCAAGATCAA CTTATTCTTC AAATCATTTC ACTCATGGAC AAGCTGTTAC GGAAAGAAAA TCTGGACTTG AAATTGACAC CTTATAAGGT GTTAGCCACC AGTACAAAAC ATGGCTTCAT GCAGTTTATC CAGTCAGTTC CTGTGGCTGA AGTTCTTGAT ACAGAGGGAA GCATTCAGAA CTTTTTTAGA AAATATGCAC CAAGTGAGAA TGGGCCAAAT GGGATTAGTG CTGAGGTCAT GGACACTTAC GTTAAAAGCT GTGCTGGATA TTGCGTGATC ACCTATATAC TTGGAGTTGG AGACAGGCAC CTGGATAACC TTTTGCTAAC AAAAACAGGC AAACTCTTCC ACATAGACTT TGGATATATT TTGGGTCGGG ATCCAAAGCC TCTTCCTCCA CCAATGAAGC TGAATAAAGA AATGGTAGAA GGAATGGGGG GCACACAGAG TGAGCAGTAC CAAGAGTTCC GTAAACAGTG TTACACGGCT TTCCTCCACC TGCGAAGGTA TTCTAATCTG ATTTTGAACT TGTTTTCCTT GATGGTTGAT GCAAACATTC CAGATATTGC ACTTGAACCA GATAAAACTG TGAAAAAGGT TCAGGATAAA TTCCGCTTAG ACCTGTCGGA TGAAGAGGCT GTGCATTACA TGCAGAGTCT GATTGATGAG AGTGTCCATG CTCTTTTTGC TGCAGTGGTG GAACAGATTC ACAAGTTTGC CCAGTACTGG AGAAAAACTA GTACAAGTTA TCCATATGAT AATCCAGATT ATGCACCAGT TGCAACATTA GGTACCATGG TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAGAC CATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCTGAGG TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG ACGAGCTGTA CAAGCCCGGG TCGAGGGATA TGGCAGCTTA ATGTTCGTTT TTCTTATTTA TATATTTATA CCAATTGATT GTATTTATAA CTGTAAAAAT GTGTATGTTG TGTGCATATT TTTTTTTGTG CATGCACATG CATGTAAATA GCTAAAATTA TGAACATTTT ATTTTTTGTT CAGAAAAAAA AAACTTTACA CACATAAAAT GGCTAGTATG AATAGCCATA TTTTATATAA ATTAAATCCT ATGAATTTAT GACCATATTA AAAATTTAGA TATTTATGGA ACATAATATG TTTGAAACAA TAAGACAAAA TTATTATTAT TATTATTATT TTTACTGTTA TAATTATGTG TCTCCTTCAA TGATTCATAA ATAGTTGGAC TTGATTTTTA AAATGTTTAT AATATGATTA GCATAGTTAA ATAAAAAAAG TTGAAAAATT AAAAAAAAAC ATATAAACAC AAATGATGGT TTTTCCTTCA ATTTCGATAT CAATTTATAG AAACAAAATA TATACTTGTA TAATTTTATT TTTTTATATA AATCATTACA TATATAATTA TACAATATTT TTTCTAAGAG ATAATTATAT ATTAATATAT ATAAAAAAAG GTGTTTTTTT TTTTTTTTTT TATTTTTATT TTTATTTTAT GGTAATATTT TATTTTCCTT ATTTTATAAA TTATATTAGT TTATATGTGA TTAATTTTAT ATATTATCAA TTTATATATT TTTAAATGCT TACTTAATTA TCTTTTTTTT TTTTTTTTTT TTTTTTTCCC CTCTTTTTAT ATTAATTTAT TTTTGAAAAA ATTGATATAT ATATATATAT ATAATATATA TATATACATG TAGTAGTATT AAACAATGTA TAATATATAT AAATAATATA TTTATATATT TCATTTCAAT TTTAATTTTT TTTGGTTTTT TTTTTTTTTC TTTTTGTCAT ATTTAAAAAA AATTATATTC ATATAAGTTA TGCATTTTTT ATAAACATTA TTCAATATAT GTATAATATA ATATATATAT ATATATTAAT GTATTATTCC AATGTGCATG ATAAAAGAAA AAAATAATAT TTATAAAAAA AAAGAAAAAT AAAACAAAAA AAGAAAAAAA AAAAAAAAAA AAAAAAAATA CAAAAATAAA TAATATAATT TATAATTATA TATTCTTGTC ACAATAAAAA TATATATATA TATATATATA TTTATAATAT GTATATTTTA AACTAGAAAA GGAATAACTA ATATTTTATT TATTATCATT CAAGATTTAT ATTTTATAAT AATAAATACC TAATAGAAAT ATATCAGGAT CCATGGCCAA GCCTTTGTCT CAAGAAGAAT CCACCCTCAT TGAAAGAGCA ACGGCTACAA TCAACAGCAT CCCCATCTCT GAAGACTACA GCGTCGCCAG CGCAGCTCTC TCTAGCGACG GCCGCATCTT CACTGGTGTC AATGTATATC ATTTTACTGG GGGACCTTGT GCAGAACTCG TGGTGCTGGG CACTGCTGCT GCTGCGGCAG CTGGCAACCT GACTTGTATC GTCGCGATCG GAAATGAGAA CAGGGGCATC TTGAGCCCCT GCGGACGGTG CCGACAGGTG CTTCTCGATC TGCATCCTGG GATCAAAGCG ATAGTGAAGG ACAGTGATGG ACAGCCGACG GCAGTTGGGA TTCGTGAATT GCTGCCCTCT GGTTATGTGT GGGAGGGCTA AAAGCTTATT TAATAATAGA TTAAAAATAT TATAAAAATA AAAACATAAA CACAGAAATT ACAAAAAAAA TACATATGAA TTTTTTTTTG TAATCTTCCT TATAAATATA GAATAATGAA TCATATAAAA CATATCATTA TTCATTTATT TACATTTAAA ATTATTGTTT CAGTATCTTT AATTTATTAT GTATATATAA AAATAACTTA CAATTTTATT AATAAACAAT ATATGTTTAT TAATTCATGT TTTGTAATTT ATGGGATAGC GATTTTTTTT ACTGTCTGTA TTTTTCTTTT TTAATTATGT TTTAATTGTA TTTTATTTTT ATTATTGTTC TTTTTATAGT ATTATTTTAA AACAAAATGT ATTTTCTAAG AACTTATAAT AATAATAAAT ATAAATTTTA ATAAAAATTA TATTTATCTT TTACAATATG AACATAAAGT ACAACATTAA TATATAGCTT TTAATATTTT TATTCCTAAT CATGTAAATC TTAAATTTTT CTTTTTAAAC ATATGTTAAA TATTTATTTC TCATTATATA TAAGAACATA TTTATTAAAT CTAGAATTCT ATAGTGAGTC GTATTACAAT TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGC CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATCGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTATTT AGGTGACACT ATAGAATACT C
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
