NM_133187.3;1110032E23Rik; 2210419I08Rik; Ened;Fam198b; GaskbB
100μg liquid plasmid(Instantly):$91
| name | pHBAd-U6-MCS-CMV-Gask1b(mouse)-P2A-EGFP | another name | apHBAd-U6-MCS-CMV-Gask1b(mouse)-P2A-EGFP |
| Insert Size (bp) | 1554bp | Plasmid host | Mammalian cell,Adenovirus |
| Plasmid uses | Protein expression,Interfere with silence | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTCATCAATA ATATACCTTA TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGNTCTAGA CTCGAGCGGC CGCCCCCTTC ACCGAGGGCC TATTTCCCAT GATTCCTTCA TATTTGCATA TACGATACAA GGCTGTTAGA GAGATAATTG GAATTAATTT GACTGTAAAC ACAAAGATAT TAGTACAAAA TACGTGACGT AGAAAGTAAT AATTTCTTGG GTAGTTTGCA GTTTTAAAAT TATGTTTTAA AATGGACTAT CATATGCTTA CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAACA CCGGTCCGCA GAATTCGGAT CCATTAGGCG GCCGCGTGGA TAACCGTATT ACCGCCATGC ATTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCAC GCGTCCAGGT ACCGCCACCA TGACCTGTCC AGACAAGCCG GGGCAGCTCG TAAACTGGTT TGTCTGCTCT CTGTGCGCCC CACGAGTGTG TAAACTTTGG AGTAGTCGGC GTCCCAGGAC CAGACGGAAC CTCCTGCTGG GCACTGCCTG TGCCATCTAC CTGGGCTTTC TGGTGAGCCA GGTGGGCAGA GGTTCCTTTC AGCATGGGCA GGCGACTGAC AGGGGACCAC CCAACGGTCA CGACATCTTC AAGGTACCCT TCTCTGAGAT TCCCCTGGAT GGTACCCTGG CCCCTCCAGA GTTGCAGGGC AATGGAAGCA CTTTGCAGCC CAACGTGGTG TACATTACTC TACGCTCCAA GCGCAGCAAG CCTGCGAATA TCCGTGGCAC GGTGAAACCC AAGCGCAGGA AGAAGTATGC GGTGGCATCT GCAGCCCCTG ATCAGGAGGT GCTGGTTAGA CCATCCCTTA TACAGCAGGA AGCTGCACGG GCAGCAGATG CTGAAGTACC TGGCTATGTT CAGGGTTACC TAACCAAGGT TGGTGAACGA CCCTGGAGGG TGCTGCGTGG TCCTGGAGTC AGAACTAGAG GTTCAAACTT GCAGCAGCCT AGGGCCCGAG AGAGCAACAT TAGGATTTAT AGCGAGAGCG CCCCCTCATG GCTGAGCAAA GAAGACATCC GCAGAATGCG CCTCCTGGCT GATAGTGAGG TGGCTAGCAT CCTACCTATC TCTAAGAGTG GTACCCGCTT GCTGGTGTTG GAAGGGAGCA CCTCAGGCTC TGTGCCTGGC TGCGGGCCTA GCCCCTGTGG GCTTCTCAAG CAGCCGCTGG ACATGAGTGA AGTGTTCGCC TTCCACCTGG ACAGGATCCT GGGTCTCAAC AGGACCCTGC CATCTGTGAG CAGGAAATTA GAGTTCATCC AAGATGGCCG ACCTCGCCCT ATCATTCTCT GGGACTCATC TCTAGCATCT GCAAGCAATG ATAGCCATTC CTCTGTTAAG ATCACCTGGG GAACTTATCA GCGGTTGCTA AAACAGAAGT GCTGGCTGAA TGGCCGGGTT CCCAGACCTG AGTGGGACTG CACTGAAATC CATCACCACG AGTGGTCCAA GATGGCTCTC TTTGATTTTT TGTTACAGAT TTATAATCGC TTGGATACAA ATTGCTGCGG ATTCCGACCT CGAAAGGAAG ATGCCTGTAT ACAGAATGGA CTGCGGTCGA ACTGTGAGGA CCAAACTTCT GTGACGTTAG CACACATTAT CCAGAGAAAG AATGACCCAA GGCATTTGGT CTTCATAAAC AATAAGGGCT TCTTTGATAG AAGCGAAGAT AACTTAAACT TTAAGTTGTT AGAAGGCATC CGAGAGTTTC CTGAATCTGC AGTTTCTGTT TTGAAGAGCC AACACTTAAG ACAGAAGCTC CTTCAGTCTC TGTTCCTTGA TCAGGTTTAT TGGGAAAGTC AAGGAGGCAG GCAAGGCATC GAGAAGCTTA TCGACGTGAT AGAGCGGAGA GCCAGAATCC TTATCACCTA CATCAACGCA CACGGGGCTA GAGTATTACC TATGAATGAA GATATCGCCA CTAACTTCTC CCTGTTGAAA CAAGCAGGGG ATGTCGAAGA GAATCCCGGG CCACCGGTCG CCACCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAGCTTG AGCTCGTCGA CTTCGAGCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG ATCGTCTAGC ATCGAAGATC CAATAACTTC GTATAGCATA CATTATACGA AGTTATAAGT AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAACACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACTCTAG GCAAAATAGC ACCCTCCCGC TCCAGAACAA CATACAGCGC TTCACAGCGG CAGCCTAACA GTCAGCCTTA CCAGTAAAAA AGAAAACCTA TTAAAAAAAC ACCACTCGAC ACGGCACCAG CTCAATCAGT CACAGTGTAA AAAAGGGCCA AGTGCAGAGC GAGTATATAT AGGACTAAAA AATGACGTAA CGGTTAAAGT CCACAAAAAA CACCCAGAAA ACCGCACGCG AACCTACGCC CAGAAACGAA AGCCAAAAAA CCCACAACTT CCTCAAATCG TCACTTCCGT TTTCCCACGT TACGTAACTT CCCATTTTAA GAAAACTACA ATTCCCAACA CATACAAGTT ACTCCGCCCT AAAACCTACG TCACCCGCCC CGTTCCCACG CCCCGCGCCA CGTCACAAAC TCCACCCCCT CATTATCATA TTGGCTTCAA TCCAAAATAA GGTATA
