pT3-EF1a-PRMT5(human)-3×Myc
$70.00
In stock
SKU
PL43176
NM_006109.5;HRMT1L5; HSL7; IBP72; JBP1; SKB1; SKB1Hs
100μg liquid plasmid(Instantly):$91
| name | pT3-EF1a-PRMT5(human)-3×Myc | another name | apT3-EF1a-PRMT5(human)-3×Myc |
| Insert Size (bp) | 1914bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | EF1a | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT ACTTCCACCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTAGCTT GGTACTAATA CGACTCACTA TAGGGAGACC CAAGCTGGCT AGGTAAGCTT GGTACCGAGC TCGGATCCAC TAGTCCAGTG TGGTGGAATT CTGCAGATAT CAACAAGTTT GTACAAAAAA GCAGGCTCCG CGGCCGCCCC CTTCACCATG GCGGCGATGG CGGTCGGGGG TGCTGGTGGG AGCCGCGTGT CCAGCGGGAG GGACCTGAAT TGCGTCCCCG AAATAGCTGA CACACTAGGG GCTGTGGCCA AGCAGGGGTT TGATTTCCTC TGCATGCCTG TCTTCCATCC GCGTTTCAAG AGGGAGTTCA TTCAGGAACC TGCTAAGAAT CGGCCCGGTC CCCAGACACG ATCAGACCTA CTGCTGTCAG GAAGGGACTG GAATACGCTA ATTGTGGGAA AGCTTTCTCC ATGGATTCGT CCAGACTCAA AAGTGGAGAA GATTCGCAGG AACTCCGAGG CGGCCATGTT ACAGGAGCTG AATTTTGGTG CATATTTGGG TCTTCCAGCT TTCCTGCTGC CCCTTAATCA GGAAGATAAC ACCAACCTGG CCAGAGTTTT GACCAACCAC ATCCACACTG GCCATCACTC TTCCATGTTC TGGATGCGGG TACCCTTGGT GGCACCAGAG GACCTGAGAG ATGATATAAT TGAGAATGCA CCAACTACAC ACACAGAGGA GTACAGTGGG GAGGAGAAAA CGTGGATGTG GTGGCACAAC TTCCGGACTT TGTGTGACTA TAGTAAGAGG ATTGCAGTGG CTCTTGAAAT TGGGGCTGAC CTCCCATCTA ATCATGTCAT TGATCGCTGG CTTGGGGAGC CCATCAAAGC AGCCATTCTC CCCACTAGCA TTTTCCTGAC CAATAAGAAG GGATTTCCTG TTCTTTCTAA GATGCACCAG AGGCTCATCT TCCGGCTCCT CAAGTTGGAG GTGCAGTTCA TCATCACAGG CACCAACCAC CACTCAGAGA AGGAGTTCTG CTCCTACCTC CAATACCTGG AATACTTAAG CCAGAACCGT CCTCCACCTA ATGCCTATGA ACTCTTTGCC AAGGGCTATG AAGACTATCT GCAGTCCCCG CTTCAGCCAC TGATGGACAA TCTGGAATCT CAGACATATG AAGTGTTTGA AAAGGACCCC ATCAAATACT CTCAGTACCA GCAGGCCATC TATAAATGTC TGCTAGACCG AGTACCAGAA GAGGAGAAGG ATACCAATGT CCAGGTACTG ATGGTGCTGG GAGCAGGACG GGGACCCCTG GTGAACGCTT CCCTGCGGGC AGCCAAGCAG GCCGACCGGC GGATAAAGCT GTATGCTGTG GAGAAAAACC CAAATGCCGT GGTGACGCTA GAGAACTGGC AGTTTGAAGA ATGGGGAAGC CAAGTGACCG TAGTCTCATC AGACATGAGG GAATGGGTGG CTCCAGAGAA AGCAGACATC ATTGTCAGTG AGCTTCTGGG CTCATTTGCT GACAATGAAT TGTCGCCTGA GTGCCTGGAT GGAGCCCAGC ACTTCCTAAA AGATGATGGT GTGAGCATCC CCGGGGAGTA CACTTCCTTT CTGGCTCCCA TCTCTTCCTC CAAGCTGTAC AATGAGGTCC GAGCCTGTAG GGAGAAGGAC CGTGACCCTG AGGCCCAGTT TGAGATGCCT TATGTGGTAC GGCTGCACAA CTTCCACCAG CTCTCTGCAC CCCAGCCCTG TTTCACCTTC AGCCATCCCA ACAGAGATCC TATGATTGAC AACAACCGCT ATTGCACCTT GGAATTTCCT GTGGAGGTGA ACACAGTACT ACATGGCTTT GCCGGCTACT TTGAGACTGT GCTTTATCAG GACATCACTC TGAGTATCCG TCCAGAGACT CACTCTCCTG GGATGTTCTC ATGGTTTCCC ATCCTCTTCC CTATTAAGCA GCCCATAACG GTACGTGAAG GCCAAACCAT CTGTGTGCGT TTCTGGCGAT GCAGCAATTC CAAGAAGGTG TGGTATGAGT GGGCTGTGAC AGCACCAGTC TGTTCTGCTA TTCATAACCC CACAGGCCGC TCATATACCA TTGGCCTCCC ATGGGAACAA AAACTAATAT CAGAGGAAGA TTTGGAACAG AAACTAATCA GTGAAGAAGA TTTAGAGCAA AAGTTAATTT CCGAGGAGGA CTTATAGTTT AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAATAAC TTCGTATAAT GTATGCTATA CGAAGTTATA TCGTATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGA ATTCCTTGAA ATACATCCAC AGGTACACCT CCAATTGACT CAAATGATGT CAATTAGTCT ATCAGAAGCT TCTAAAGCCA TGACATCATT TTCTGGAATT TTCCAAGCTG TTTAAAGGCA CAGTCAACTT AGTGTATGTA AACTTCTGAC CCACTGGAAT TGTGATACAG TGAATTATAA GTGAAATAAT CTGTCTGTAA ACAATTGTTG GAAAAATGAC TTGTGTCATG CACAAAGTAG ATGTCCTAAC TGACTTGCCA AAACTATTGT TTGTTAACAA GAAATTTGTG GAGTAGTTGA AAAACGAGTT TTAATGACTC CAACTTAAGT GTATGTAAAC TTCCGACTTC AACTGTAGGG TACCGAGCTC GAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT TGCATGCCTG CAGGTCGACT CTAGACCTAT ACAGTTGAAG TCGGAAGTTT ACATACACTT AAGTTGGAGT CATTAAAACT CGTTTTTCAA CTACTCCACA AATTTCTTGT TAACAAACAA TAGTTTTGGC AAGTCAGTTA GGACATCTAC TTTGTGCATG ACACAAGTCA TTTTTCCAAC AATTGTTTAC AGACAGATTA TTTCACTTAT AATTCACTGT ATCACAATTC CAGTGGGTCA GAAGTTTACA TACACTAAGT TGACTGTGCC TTTAAACAGC TTGGAAAATT CCAGAAAATG ATGTCATGGC TTTAGAAGCT TCTGATAGAC TAATTGACAT CATTTGAGTC AATTGGAGGT GTACCTGTGG ATGTATTTCA AGGGGATCCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GCGGCCGCTA GCTCGAGATC TTAATTAAAC TAGTATCGAT ATAACTTCGT ATAATGTATG CTATACGAAG TTATATGTAC GGGCCAGATA TACGCGTTGA CATTGATTAT TGACTAGGCT TTTGCAAAAA GCTTTGCAAA GATGGATAAA GTTTTAAACA GAGAGGAATC TTTGCAGCTA ATGGACCTTC TAGGTCTTGA AAGGAGTGGG AATT
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