pCMV-PHLPP2(human)-Neo
$99.00
In stock
SKU
PL43173
NM_015020.3;PHLPPL; PPM3B
100μg liquid plasmid(Instantly):$120
| name | pCMV-PHLPP2(human)-Neo | another name | apCMV-PHLPP2(human)-Neo |
| Insert Size (bp) | 3972bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC CACTAGTCCA GTGTGGTGGA ATTCGCCACC ATGAAACGCA ATGGGAGCAG AAATTGTTTG AATAGGAGAA GTAGGTTTGG TTCTCGAGAA AGAGACTGGC TAAGAGAAGA TGTAAAGAGA GGCTGTGTTT ACCTTTATGG AGCAGACACT ACCACTGCCA CTACAACCAC CACCACCTCC TCTTCCTCTT CCTCCTCCTC CTCTTCCTCT GACTTACATC TCGTCCTTTG CACTGTAGAG ACACCAGCAT CAGAAATATG TGCTGGAGAG GGAAGAGAAA GTCTTTATTT ACAGCTTCAT GGAGACCTGG TCAGGAGACT GGAACCTACT GAACGACCTC TTCAGATCGT TTATGATTAC TTATCCAGGC TGGGATTTGA TGATCCTGTG CGCATACAGG AGGAGGCTAC AAATCCTGAC CTCGGCTGTA TGATTCGATT TTATGGTGAA AAACCATGCC ACATGGATCG TTTGGATCGA ATCCTATTGT CTGGCATCTA TAATGTACGC AAGGGAAAGA CCCAGCTGCA TAAGTGGGCT GAGCGCCTAG TTGTCCTCTG TGGTACCTGC CTTATCGTTT CCTCAGTGAA GGATTGTCAA ACTGGAAAGA TGCACATTTT GCCTCTGGTT GGTGGAAAGA TAGAAGAAGT GAAGCGACGG CAATACTCCC TTGCTTTCAG CTCAGCAGGA GCCCAAGCTC AGACCTATCA TGTCAGCTTC GAGACTTTGG CCGAGTACCA GCGATGGCAA CGGCAAGCAT CCAAGGTGGT GTCCCAGCGA ATCAGTACCG TGGATCTCTC GTGTTACAGC CTCGAGGAGG TTCCTGAGCA TCTCTTCTAT AGTCAAGATA TTACCTACCT CAACTTGCGA CACAACTTCA TGCAGTTAGA AAGACCCGGA GGCCTCGATA CACTCTACAA ATTTTCTCAA CTGAAGGGCC TGAACTTGTC CCATAATAAA CTTGGGTTGT TTCCTATATT GTTATGCGAG ATCTCTACCC TGACTGAGCT CAACCTTTCC TGTAATGGAT TTCATGACCT ACCAAGTCAA ATTGGCAATC TGCTAAATCT TCAAACCCTC TGTCTTGATG GCAACTTTCT GACTACTTTA CCTGAAGAAT TGGGAAATCT ACAACAGCTT TCCTCCTTGG GAATTTCCTT CAACAACTTT AGTCAAATTC CTGAGGTTTA TGAGAAACTC ACTATGTTAG ATAGAGTGGT TATGGCAGGA AATTGCCTGG AAGTCCTGAA CTTAGGGGTG CTGAATAGGA TGAACCATAT CAAGCATGTG GATTTAAGGA TGAACCATTT GAAAACCATG GTTATTGAAA ATCTGGAGGG AAATAAACAC ATCACCCACG TGGATCTGCG GGACAACCGA CTGACTGACT TGGATCTTAG CTCCTTATGC AGCTTGGAAC AGCTGCACTG TGGGCGGAAT CAGCTGAGGG AGCTAACACT CAGTGGCTTT TCCCTTCGGA CCCTCTATGC CAGTTCCAAC AGGCTGACAG CAGTGAACGT CTATCCAGTA CCCAGCCTGC TCACTTTCTT GGATCTCTCC CGAAACCTGC TAGAGTGTGT CCCTGACTGG GCCTGTGAAG CAAAGAAGAT AGAAGTATTA GATGTGAGCT ATAATCTTCT CACAGAGGTT CCCGTGAGAA TTCTGAGTAG CTTGAGTCTT AGAAAACTGA TGCTGGGACA CAATCATGTG CAAAACCTTC CAACACTGGT AGAGCACATC CCCCTCGAGG TGCTGGATCT TCAGCATAAT GCACTCACGA GGCTGCCAGA CACCCTCTTC TCCAAGGCCT TAAATCTCAG ATACTTGAAT GCATCTGCAA ATAGTCTGGA GTCTTTACCA TCCGCCTGCA CTGGAGAGGA GAGTTTGAGT ATGCTGCAGC TGCTTTATCT GACCAACAAT CTCCTGACGG ATCAGTGCAT ACCTGTCCTG GTAGGGCACC TGCACCTGCG AATCTTGCAC CTTGCAAACA ATCAGTTACA GACCTTTCCT GCAAGCAAAC TAAATAAATT GGAGCAATTG GAGGAACTGA ACCTAAGTGG CAACAAGCTT AAAACCATTC CCACAACCAT AGCAAACTGT AAAAGGCTGC ACACCCTTGT TGCACACTCC AACAACATCA GCATTTTCCC AGAAATACTG CAGTTGCCTC AGATCCAGTT TGTAGACCTA AGTTGCAACG ACTTGACAGA AATCCTGATT CCAGAGGCTT TGCCTGCTAC ATTACAAGAC CTTGACCTGA CTGGAAATAC AAATCTGGTT CTGGAACACA AGACACTGGA CATATTTAGC CATATCACAA CCCTGAAAAT TGATCAGAAA CCTTTGCCAA CCACAGATTC TACAGTTACG TCAACCTTCT GGAGCCATGG ACTGGCTGAG ATGGCAGGGC AGAGAAATAA GCTGTGTGTC TCAGCACTTG CTATGGATAG CTTTGCAGAG GGGGTGGGAG CTGTGTATGG CATGTTTGAT GGAGACCGAA ATGAGGAGCT CCCGCGCCTG CTGCAGTGTA CGATGGCAGA TGTGCTTTTA GAAGAGGTAC AGCAGTCAAC TAATGACACA GTTTTCATGG CTAACACCTT CTTGGTATCT CACAGGAAAT TAGGAATGGC TGGCCAGAAG TTGGGCTCCT CCGCTCTCCT GTGCTACATC CGCCCTGACA CTGCCGATCC AGCAAGTAGC TTTAGCTTGA CTGTAGCCAA TGTTGGCACG TGCCAAGCAG TCCTGTGCCG AGGTGGGAAG CCAGTGCCCC TCTCTAAAGT CTTCAGCCTG GAGCAGGACC CAGAGGAGGC TCAAAGGGTG AAGGACCAAA AAGCCATCAT CACAGAGGAC AACAAAGTGA ATGGGGTAAC CTGCTGTACC CGGATGCTGG GCTGTACATA CCTCTACCCT TGGATCCTCC CCAAGCCCCA CATATCTTCC ACTCCGCTGA CCATTCAAGA TGAGTTGCTG ATTCTGGGAA ACAAAGCATT GTGGGAACAC TTGTCCTACA CAGAAGCTGT CAATGCTGTA CGTCACGTAC AAGACCCATT AGCAGCTGCT AAGAAGCTGT GCACATTAGC GCAGAGCTAT GGCTGTCAGG ACAATGTAGG GGCGATGGTA GTTTATTTGA ATATTGGTGA GGAAGGCTGC ACTTGTGAAA TGAATGGGCT CACCCTCCCA GGTCCTGTGG GATTTGCTTC AACCACCACT ATCAAGGATG CCCCTAAGCC AGCCACTCCA TCCTCTAGCA GTGGGATTGC CTCTGAGTTC AGCAGTGAGA TGTCCACCTC AGAGGTGAGC AGTGAAGTGG GGTCCACTGC TTCTGATGAG CATAATGCTG GGGGCCTGGA CACTGCCTTG CTTCCGAGGC CAGAGCGGCG CTGCAGCCTC CACCCAACAC CCACCTCTGG GCTGTTTCAG CGCCAGCCTT CTTCTGCTAC CTTCTCCAGT AACCAGTCTG ACAACGGCCT GGACAGTGAT GATGACCAGC CCGTTGAGGG GGTCATAACC AATGGCAGCA AGGTAGAGGT GGAAGTAGAC ATCCACTGCT GCAGGGGGAG GGATCTGGAG AACTCACCCC CTCTCATAGA GAGTTCTCCT ACCCTGTGTT CTGAGGAACA TGCTAGAGGG TCGTGTTTTG GGATCCGAAG ACAGAACAGT GTGAATAGTG GCATGCTCCT GCCAATGAGC AAGGACAGGA TGGAGTTACA GAAGTCTCCC TCCACCTCCT GCCTCTATGG GAAGAAACTC TCCAATGGCT CTATTGTGCC CCTAGAGGAC AGCCTGAACC TCATTGAAGT GGCCACAGAA GTGCCCAAGA GGAAAACTGG CTATTTTGCT GCCCCCACTC AGATGGAACC AGAGGACCAG TTTGTTGTGC CTCATGACCT GGAAGAAGAA GTGAAGGAAC AAATGAAACA GCACCAGGAC AGCCGGCTCG AGCCTGAGCC CCATGAAGAG GATCGGACCG AGCCCCCGGA GGAGTTCGAC ACAGCACTAT GACTCGAGTC TAGAGGGCCC GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGT
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