pT7-6×His-HCFC1(human)
$142.00
In stock
SKU
PL43170
NM_005334.3;CFF; HCF; HCF-1; HCF1; HFC1; MRX3; PPP1R89
100μg liquid plasmid(Instantly):$185
| name | pT7-6×His-HCFC1(human) | another name | apT7-6×His-HCFC1(human) |
| Insert Size (bp) | 6105bp | Plasmid host | Escherichia coli |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | ||
| Promoter | T7 | Replicon | pBR322 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | Low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC AGCAGCGGCC TGGTGCCGCG CGGCAGCCAT ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGCGGAT CCATGGCTTC GGCCGTGTCG CCCGCCAACT TGCCAGCGGT GCTTCTGCAG CCCCGCTGGA AGCGAGTGGT GGGCTGGTCG GGTCCGGTGC CACGGCCCCG CCACGGCCAC CGCGCCGTGG CCATCAAGGA GCTCATCGTG GTGTTTGGCG GCGGCAACGA GGGAATAGTG GACGAACTGC ACGTGTACAA CACGGCAACC AACCAGTGGT TCATCCCAGC CGTGAGGGGG GACATTCCCC CTGGGTGTGC AGCCTATGGC TTCGTGTGTG ACGGGACTCG CCTCCTGGTG TTTGGTGGGA TGGTGGAGTA TGGGAAATAC AGCAATGACC TCTACGAACT CCAGGCGAGC CGGTGGGAGT GGAAGAGACT CAAAGCAAAG ACGCCCAAAA ACGGGCCCCC TCCGTGTCCT CGACTCGGGC ACAGCTTCTC CCTTGTGGGC AACAAATGCT ACCTGTTTGG GGGTCTGGCC AATGATAGCG AGGACCCAAA GAACAACATT CCAAGGTACC TGAATGACTT ATATATCCTG GAATTACGGC CAGGCTCTGG AGTGGTAGCC TGGGACATTC CCATCACTTA CGGGGTCCTA CCACCACCCC GGGAGTCACA TACTGCCGTG GTCTACACCG AAAAAGACAA TAAGAAGTCC AAGCTGGTGA TCTACGGCGG GATGAGTGGC TGCAGGCTGG GGGACCTGTG GACCCTAGAT ATTGACACCC TGACGTGGAA TAAGCCCAGT CTCAGCGGGG TGGCGCCTCT TCCTCGCAGT CTCCACTCGG CAACCACCAT CGGAAATAAA ATGTACGTGT TTGGTGGCTG GGTGCCTCTC GTCATGGATG ACGTCAAAGT GGCCACACAC GAGAAGGAGT GGAAGTGTAC CAACACGCTG GCTTGTCTCA ACCTGGATAC CATGGCCTGG GAGACCATCC TGATGGATAC ACTGGAGGAC AACATCCCCC GTGCTCGGGC TGGCCACTGC GCAGTCGCCA TCAACACCCG CCTGTACATT TGGAGTGGGC GTGACGGCTA CCGCAAGGCC TGGAACAACC AGGTCTGCTG CAAGGACCTC TGGTACCTAG AGACAGAAAA GCCACCACCC CCAGCCCGAG TACAACTGGT ACGCGCCAAC ACCAACTCCC TGGAGGTGAG CTGGGGGGCA GTGGCAACAG CCGACAGCTA CCTTCTCCAG CTCCAGAAAT ATGACATTCC TGCCACGGCT GCTACTGCCA CCTCCCCTAC ACCCAATCCG GTCCCATCTG TGCCTGCCAA CCCTCCCAAG AGCCCTGCCC CAGCAGCAGC CGCACCTGCT GTGCAGCCGC TGACCCAAGT AGGCATCACG CTCCTGCCCC AGGCTGCCCC CGCACCCCCG ACCACCACCA CCATCCAGGT CTTGCCAACG GTGCCTGGCA GCTCCATTTC TGTGCCCACC GCAGCCAGGA CTCAAGGTGT CCCTGCTGTT CTCAAAGTGA CCGGTCCTCA GGCTACAACA GGAACTCCAT TGGTCACCAT GCGACCTGCC AGCCAGGCTG GGAAAGCCCC TGTCACCGTG ACCTCCCTTC CCGCCGGAGT GCGGATGGTT GTGCCAACAC AGAGTGCCCA GGGAACGGTG ATTGGCAGTA GCCCACAGAT GAGTGGGATG GCCGCACTGG CCGCTGCGGC CGCTGCCACC CAGAAGATCC CCCCTTCCTC GGCACCCACG GTGCTGAGTG TCCCAGCGGG TACCACCATC GTGAAGACCA TGGCTGTGAC ACCTGGCACT ACCACCCTCC CAGCCACTGT GAAGGTGGCC TCCTCGCCAG TCATGGTGAG CAACCCTGCC ACTCGCATGC TGAAGACTGC AGCCGCCCAG GTGGGGACAT CGGTTTCCTC CGCCACCAAC ACGTCTACCC GCCCTATCAT CACAGTGCAC AAGTCAGGCA CTGTGACAGT GGCCCAGCAA GCCCAGGTGG TGACCACAGT TGTGGGCGGG GTCACCAAGA CCATCACCCT GGTGAAGAGC CCCATCTCTG TCCCAGGAGG CAGTGCTCTG ATTTCCAATC TGGGCAAAGT GATGTCGGTG GTCCAGACCA AACCAGTTCA GACTTCAGCA GTCACAGGCC AGGCGTCCAC GGGTCCTGTG ACTCAGATCA TCCAGACCAA AGGGCCCCTG CCAGCGGGAA CAATCCTGAA GCTGGTGACC TCAGCAGATG GCAAGCCCAC CACCATCATC ACTACCACGC AGGCCAGTGG GGCGGGGACC AAGCCCACCA TCCTGGGCAT CAGCAGCGTC TCCCCCAGTA CCACCAAGCC CGGCACGACC ACCATCATCA AAACCATCCC CATGTCGGCC ATCATCACCC AGGCGGGCGC CACGGGTGTG ACCAGCAGTC CTGGCATCAA GTCCCCCATC ACCATCATCA CCACCAAGGT GATGACTTCA GGAACTGGAG CACCTGCGAA AATCATCACT GCTGTCCCCA AAATTGCCAC TGGCCACGGG CAGCAGGGAG TGACCCAGGT GGTGCTTAAG GGGGCCCCGG GACAGCCAGG CACCATCCTC CGCACTGTGC CCATGGGGGG TGTTCGCCTG GTCACACCCG TCACCGTCTC CGCCGTCAAG CCAGCCGTCA CCACGTTGGT TGTGAAAGGC ACCACAGGTG TCACGACCCT AGGCACAGTG ACAGGCACCG TCTCCACCAG CCTTGCCGGG GCGGGGGGCC ACAGCACTAG TGCTTCCCTG GCCACGCCCA TCACCACCTT GGGCACCATT GCCACCCTCT CAAGCCAGGT GATCAACCCC ACTGCCATCA CTGTGTCGGC CGCACAGACC ACGCTGACAG CGGCAGGCGG GCTCACAACC CCAACCATCA CCATGCAGCC CGTGTCCCAG CCCACCCAGG TAACTCTGAT CACGGCACCT AGTGGGGTGG AGGCCCAGCC TGTGCATGAC CTCCCTGTGT CCATTCTGGC CTCCCCGACT ACAGAACAGC CCACCGCCAC AGTTACCATC GCCGACTCAG GCCAGGGTGA TGTGCAGCCT GGCACTGTCA CCTTGGTGTG CTCCAACCCA CCCTGTGAGA CCCACGAGAC TGGCACCACC AACACGGCCA CCACTACTGT TGTGGCTAAC CTTGGGGGAC ACCCCCAGCC CACCCAAGTG CAGTTCGTCT GTGACAGACA GGAGGCAGCT GCTTCTCTTG TGACCTCGAC TGTGGGCCAG CAGAATGGTA GCGTGGTCCG AGTCTGTTCG AACCCGCCCT GCGAGACCCA CGAGACGGGC ACCACCAACA CCGCCACCAC CGCCACCTCC AACATGGCCG GGCAGCATGG CTGCTCAAAC CCACCCTGCG AGACCCACGA GACGGGCACC ACCAACACTG CCACTACAGC CATGTCGAGC GTCGGCGCCA ACCACCAGCG AGATGCCCGT CGGGCCTGTG CAGCTGGCAC CCCTGCCGTG ATCCGGATCA GTGTGGCCAC TGGGGCGCTG GAGGCAGCCC AGGGCTCTAA GTCCCAGTGC CAAACCCGCC AGACCAGCGC GACCAGCACC ACCATGACTG TGATGGCCAC CGGGGCCCCG TGCTCGGCCG GCCCACTCCT TGGGCCGAGC ATGGCACGGG AGCCCGGGGG CCGCAGCCCT GCTTTTGTGC AGTTGGCCCC TCTGAGCAGC AAAGTCAGGC TGAGCAGCCC AAGCATTAAG GACCTTCCTG CGGGGCGCCA CAGCCATGCG GTCAGCACCG CTGCCATGAC CCGTTCCAGC GTGGGTGCTG GGGAGCCCCG CATGGCACCT GTGTGCGAGA GCCTCCAGGG TGGCTCGCCC AGCACCACAG TGACTGTGAC AGCCCTGGAG GCACTGCTGT GCCCCTCGGC CACCGTGACC CAAGTCTGCT CCAACCCACC ATGTGAGACC CACGAGACAG GCACCACCAA CACCGCCACT ACCTCGAATG CAGGCAGCGC CCAGAGGGTG TGCTCCAACC CGCCATGCGA GACCCACGAG ACGGGCACCA CCCACACGGC CACCACCGCT ACTTCAAACG GGGGCACGGG CCAGCCCGAG GGTGGGCAGC AGCCCCCTGC TGGTCGCCCC TGTGAGACAC ACCAGACCAC TTCCACTGGC ACCACCATGT CGGTCAGCGT GGGTGCCCTG CTTCCCGACG CCACTTCTTC CCACAGGACC GTGGAGTCTG GCCTAGAGGT GGCGGCGGCA CCCAGCGTCA CCCCCCAGGC TGGCACCGCG CTGCTGGCTC CTTTCCCAAC ACAGAGGGTG TGCTCCAACC CCCCCTGTGA GACCCACGAG ACGGGCACCA CTCACACGGC CACCACTGTC ACTTCCAACA TGAGTTCAAA CCAAGACCCC CCACCTGCTG CCAGCGATCA GGGAGAGGTG GAGAGCACCC AGGGCGACAG CGTGAACATC ACCAGCTCCA GTGCCATCAC GACAACCGTG TCCTCCACAC TGACGCGGGC TGTGACCACC GTGACGCAGT CCACACCGGT CCCGGGCCCC TCTGTGCCGC CCCCAGAGGA ACTCCAGGTG TCGCCAGGTC CTCGCCAGCA GCTGCCGCCA CGGCAGCTTC TGCAGTCGGC TTCCACAGCC CTGATGGGGG AGTCCGCCGA GGTCCTGTCA GCCTCCCAGA CCCCTGAGCT CCCGGCCGCC GTGGATCTGA GCAGCACAGG GGAGCCATCT TCGGGCCAGG AGTCTGCCGG CTCTGCGGTG GTGGCCACTG TGGTGGTCCA GCCACCCCCA CCCACACAGT CCGAAGTAGA CCAGTTATCA CTTCCCCAAG AGCTAATGGC CGAGGCCCAA GCTGGCACCA CCACCCTCAT GGTAACGGGG CTCACCCCCG AGGAGCTGGC AGTGACGGCT GCTGCAGAAG CAGCTGCCCA GGCCGCAGCC ACGGAGGAAG CCCAGGCCCT GGCCATCCAG GCGGTGCTCC AGGCCGCGCA GCAGGCCGTC ATGGGCACCG GCGAGCCCAT GGACACCTCC GAGGCAGCAG CAACCGTGAC TCAGGCGGAG CTGGGGCACC TGTCGGCCGA GGGTCAGGAG GGCCAGGCCA CCACCATACC CATTGTGCTG ACACAGCAGG AGCTGGCTGC CCTGGTGCAG CAGCAGCAGC TGCAGGAGGC CCAGGCCCAG CAGCAGCATC ACCACCTCCC CACTGAGGCC CTGGCCCCTG CCGACAGTCT CAACGACCCA GCCATTGAGA GCAATTGCCT CAATGAGCTG GCCGGCACGG TCCCCAGCAC TGTGGCGCTG CTGCCCTCAA CGGCCACTGA GAGCCTGGCT CCATCCAACA CATTTGTGGC CCCCCAGCCG GTTGTGGTGG CCAGCCCAGC CAAGCTGCAG GCTGCAGCTA CCCTGACCGA AGTGGCCAAT GGCATCGAGT CCCTGGGTGT GAAGCCAGAC CTGCCGCCCC CACCCAGCAA AGCCCCCATG AAGAAGGAAA ACCAGTGGTT TGATGTGGGA GTCATTAAGG GCACCAATGT AATGGTGACA CACTATTTCC TGCCACCAGA TGATGCTGTC CCATCAGACG ATGATTTGGG CACCGTCCCT GACTATAACC AGCTGAAGAA GCAGGAGCTG CAGCCAGGCA CAGCCTATAA GTTTCGTGTT GCCGGAATCA ATGCCTGTGG CCGGGGGCCC TTCAGCGAAA TCTCAGCCTT TAAGACGTGC CTGCCTGGTT TCCCAGGGGC CCCTTGTGCC ATTAAAATCA GCAAAAGTCC GGATGGTGCT CACCTCACCT GGGAGCCACC CTCTGTGACC TCCGGCAAGA TTATCGAGTA CTCCGTGTAC CTGGCCATCC AGAGCTCACA GGCTGGGGGC GAGCTCAAGA GCTCCACCCC GGCCCAGCTG GCCTTCATGC GGGTGTACTG CGGGCCCAGC CCCTCCTGCC TGGTGCAGTC CTCCAGCCTT TCCAACGCCC ACATCGACTA CACCACCAAG CCCGCCATCA TCTTCCGCAT CGCCGCCCGC AATGAGAAGG GCTATGGCCC GGCCACACAA GTGAGGTGGC TGCAGGAAAC CAGTAAAGAC AGCTCTGGCA CCAAGCCAGC CAACAAGCGG CCCATGTCCT CTCCAGAAAT GAAATCTGCT CCAAAGAAAT CTAAGGCCGA TGGTCAGTGA CTCGAGCACC ACCACCACCA CCACTGAGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GAT
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