pCMV-SCN10A(human)-3×FLAG-Neo
$128.00
In stock
SKU
PL43080
NM_006514.4;FEPS2; Nav1.8; PN3; SNS
100μg liquid plasmid(Instantly):$149
| name | pCMV-SCN10A(human)-3×FLAG-Neo | another name | apCMV-SCN10A(human)-3×FLAG-Neo |
| Insert Size (bp) | 5871bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT TGGTACCGAG CTCGGATCCA CTAGTCCAGT GTGGTGGAAT TCGCCACCAT GGAATTCCCC ATTGGATCCC TCGAAACTAA CAACTTCCGT CGCTTTACTC CGGAGTCACT GGTGGAGATA GAGAAGCAAA TTGCTGCCAA GCAGGGAACA AAGAAAGCCA GAGAGAAGCA TAGGGAGCAG AAGGACCAAG AAGAGAAGCC TCGGCCCCAG CTGGACTTGA AAGCCTGCAA CCAGCTGCCC AAGTTCTATG GTGAGCTCCC AGCAGAACTG ATCGGGGAGC CCCTGGAGGA TCTAGATCCG TTCTACAGCA CACACCGGAC ATTTATGGTG CTGAACAAAG GGAGGACCAT TTCCCGGTTT AGTGCCACTC GGGCCCTGTG GCTATTCAGT CCTTTCAACC TGATCAGAAG AACGGCCATC AAAGTGTCTG TCCACTCGTG GTTCAGTTTA TTTATTACGG TCACTATTTT GGTTAATTGT GTGTGCATGA CCCGAACTGA CCTTCCAGAG AAAATTGAAT ATGTCTTCAC TGTCATTTAC ACCTTTGAAG CCTTGATAAA GATACTGGCA AGAGGATTTT GTCTAAATGA GTTCACGTAC CTGAGAGATC CTTGGAACTG GCTGGATTTT AGCGTCATTA CCCTGGCATA TGTTGGCACA GCAATAGATC TCCGTGGGAT CTCAGGCCTG CGGACATTCA GAGTTCTTAG AGCATTAAAA ACAGTTTCTG TGATCCCAGG CCTGAAGGTC ATTGTGGGGG CCCTGATTCA CTCAGTGAAG AAACTGGCTG ATGTGACCAT CCTCACCATC TTCTGCCTAA GTGTTTTTGC CTTGGTGGGG CTGCAACTCT TCAAGGGCAA CCTCAAAAAT AAATGTGTCA AGAATGACAT GGCTGTCAAT GAGACAACCA ACTACTCATC TCACAGAAAA CCAGATATCT ACATAAATAA GCGAGGCACT TCTGACCCCT TACTGTGTGG CAATGGATCT GACTCAGGCC ACTGCCCTGA TGGTTATATC TGCCTTAAAA CTTCTGACAA CCCGGATTTT AACTACACCA GCTTTGATTC CTTTGCTTGG GCTTTCCTCT CACTGTTCCG CCTCATGACA CAGGATTCCT GGGAACGCCT CTACCAGCAG ACCCTGAGGA CTTCTGGGAA AATCTATATG ATCTTTTTTG TGCTCGTAAT CTTCCTGGGA TCTTTCTACC TGGTCAACTT GATCTTGGCT GTAGTCACCA TGGCGTATGA GGAGCAGAAC CAGGCAACCA CTGATGAAAT TGAAGCAAAG GAGAAGAAGT TCCAGGAGGC CCTCGAGATG CTCCGGAAGG AGCAGGAGGT GCTAGCAGCA CTAGGGATTG ACACAACCTC TCTCCACTCC CACAATGGAT CACCTTTAAC CTCCAAAAAT GCCAGTGAGA GAAGGCATAG AATAAAGCCA AGAGTGTCAG AGGGCTCCAC AGAAGACAAC AAATCACCCC GCTCTGATCC TTACAACCAG CGCAGGATGT CTTTTCTAGG CCTCGCCTCT GGAAAACGCC GGGCTAGTCA TGGCAGTGTG TTCCATTTCC GGTCCCCTGG CCGAGATATC TCACTCCCTG AGGGAGTCAC AGATGATGGA GTCTTTCCTG GAGACCACGA AAGCCATCGG GGCTCTCTGC TGCTGGGTGG GGGTGCTGGC CAGCAAGGCC CCCTCCCTAG AAGCCCTCTT CCTCAACCCA GCAACCCTGA CTCCAGGCAT GGAGAAGATG AACACCAACC GCCGCCCACT AGTGAGCTTG CCCCTGGAGC TGTCGATGTC TCGGCATTCG ATGCAGGACA AAAGAAGACT TTCTTGTCAG CAGAATACTT AGATGAACCT TTCCGGGCCC AAAGGGCAAT GAGTGTTGTC AGTATCATAA CCTCCGTCCT TGAGGAACTC GAGGAGTCTG AACAGAAGTG CCCACCCTGC TTGACCAGCT TGTCTCAGAA GTATCTGATC TGGGATTGCT GCCCCATGTG GGTGAAGCTC AAGACAATTC TCTTTGGGCT TGTGACGGAT CCCTTTGCAG AGCTCACCAT CACCTTGTGC ATCGTGGTGA ACACCATCTT CATGGCCATG GAGCACCATG GCATGAGCCC TACCTTCGAA GCCATGCTCC AGATAGGCAA CATCGTCTTT ACCATATTTT TTACTGCTGA AATGGTCTTC AAAATCATTG CCTTCGACCC ATACTATTAT TTCCAGAAGA AGTGGAATAT CTTTGACTGC ATCATCGTCA CTGTGAGTCT GCTAGAGCTG GGCGTGGCCA AGAAGGGAAG CCTGTCTGTG CTGCGGAGCT TCCGCTTGCT GCGCGTATTC AAGCTGGCCA AATCCTGGCC CACCTTAAAC ACACTCATCA AGATCATCGG AAACTCAGTG GGGGCACTGG GGAACCTCAC CATCATCCTG GCCATCATTG TCTTTGTCTT TGCTCTGGTT GGCAAGCAGC TCCTAGGGGA AAACTACCGT AACAACCGAA AAAATATCTC CGCGCCCCAT GAAGACTGGC CCCGCTGGCA CATGCACGAC TTCTTCCACT CTTTCCTCAT TGTCTTCCGT ATCCTCTGTG GAGAGTGGAT TGAGAACATG TGGGCCTGCA TGGAAGTTGG CCAAAAATCC ATATGCCTCA TCCTTTTCTT GACGGTGATG GTGCTAGGGA ACCTGGTGGT GCTTAACCTG TTCATCGCCC TGCTATTGAA CTCTTTCAGT GCTGACAACC TCACAGCCCC GGAGGACGAT GGGGAGGTGA ACAACCTGCA GGTGGCCCTG GCACGGATCC AGGTCTTTGG CCATCGTACC AAACAGGCTC TTTGCAGCTT CTTCAGCAGG TCCTGCCCAT TCCCCCAGCC CAAGGCAGAG CCTGAGCTGG TGGTGAAACT CCCACTCTCC AGCTCCAAGG CTGAGAACCA CATTGCTGCC AACACTGCCA GGGGGAGCTC TGGAGGGCTC CAAGCTCCCA GAGGCCCCAG GGATGAGCAC AGTGACTTCA TCGCTAATCC GACTGTGTGG GTCTCTGTGC CCATTGCTGA GGGTGAATCT GATCTTGATG ACTTGGAGGA TGATGGTGGG GAAGATGCTC AGAGCTTCCA GCAGGAAGTG ATCCCCAAAG GACAGCAGGA GCAGCTGCAG CAAGTCGAGA GGTGTGGGGA CCACCTGACA CCCAGGAGCC CAGGCACTGG AACATCTTCT GAGGACCTGG CTCCATCCCT GGGTGAGACG TGGAAAGATG AGTCTGTTCC TCAGGTCCCT GCTGAGGGAG TGGACGACAC AAGCTCCTCT GAGGGCAGCA CGGTGGACTG CCTAGATCCT GAGGAAATCC TGAGGAAGAT CCCTGAGCTG GCAGATGACC TGGAAGAACC AGATGACTGC TTCACAGAAG GATGCATTCG CCACTGTCCC TGCTGCAAAC TGGATACCAC CAAGAGTCCA TGGGATGTGG GCTGGCAGGT GCGCAAGACT TGCTACCGTA TCGTGGAGCA CAGCTGGTTT GAGAGCTTCA TCATCTTCAT GATCCTGCTC AGCAGTGGAT CTCTGGCCTT TGAAGACTAT TACCTGGACC AGAAGCCCAC GGTGAAAGCT TTGCTGGAGT ACACTGACAG GGTCTTCACC TTTATCTTTG TGTTCGAGAT GCTGCTTAAG TGGGTGGCCT ATGGCTTCAA AAAGTACTTC ACCAATGCCT GGTGCTGGCT GGACTTCCTC ATTGTGAATA TCTCACTGAT AAGTCTCACA GCGAAGATTC TGGAATATTC TGAAGTGGCT CCCATCAAAG CCCTTCGAAC CCTTCGCGCT CTGCGGCCAC TGCGGGCTCT TTCTCGATTT GAAGGCATGC GGGTGGTGGT GGATGCCCTG GTGGGCGCCA TCCCATCCAT CATGAATGTC CTCCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGTGTGAA CCTCTTCGCA GGGAAGTTTT GGAGGTGCAT CAACTATACC GATGGAGAGT TTTCCCTTGT ACCTTTGTCG ATTGTGAATA ACAAGTCTGA CTGCAAGATT CAAAACTCCA CTGGCAGCTT CTTCTGGGTC AATGTGAAAG TCAACTTTGA TAATGTTGCA ATGGGTTACC TTGCACTTCT GCAGGTGGCA ACCTTTAAAG GCTGGATGGA CATTATGTAT GCAGCTGTTG ATTCCCGGGA GGTCAACATG CAACCCAAGT GGGAGGACAA CGTGTACATG TATTTGTACT TTGTCATCTT CATCATTTTT GGAGGCTTCT TCACACTGAA TCTCTTTGTT GGGGTCATAA TTGACAACTT CAATCAACAG AAAAAAAAGT TAGGGGGCCA GGACATCTTC ATGACAGAGG AGCAGAAGAA ATACTACAAT GCCATGAAGA AGTTGGGCTC CAAGAAGCCC CAGAAGCCCA TCCCACGGCC CCTGAACAAG TTCCAGGGTT TTGTCTTTGA CATCGTGACC AGACAAGCTT TTGACATCAC CATCATGGTC CTCATCTGCC TCAACATGAT CACCATGATG GTGGAGACTG ATGACCAAAG TGAAGAAAAG ACGAAAATTC TGGGCAAAAT CAACCAGTTC TTTGTGGCCG TCTTCACAGG CGAATGTGTC ATGAAGATGT TCGCTTTGAG GCAGTACTAC TTCACAAATG GCTGGAATGT GTTTGACTTC ATTGTGGTGG TTCTCTCCAT TGCGAGCCTG ATTTTTTCTG CAATTCTTAA GTCACTTCAA AGTTACTTCT CCCCAACGCT CTTCAGAGTC ATCCGCCTGG CCCGAATTGG CCGCATCCTC AGACTGATCC GAGCGGCCAA GGGGATCCGC ACACTGCTCT TTGCCCTCAT GATGTCCCTG CCTGCCCTCT TCAACATCGG GCTGTTGCTA TTCCTTGTCA TGTTCATCTA CTCTATCTTC GGTATGTCCA GCTTTCCCCA TGTGAGGTGG GAGGCTGGCA TCGACGACAT GTTCAACTTC CAGACCTTCG CCAACAGCAT GCTGTGCCTC TTCCAGATTA CCACGTCGGC CGGCTGGGAT GGCCTCCTCA GCCCCATCCT CAACACAGGG CCCCCCTACT GTGACCCCAA TCTGCCCAAC AGCAATGGCA CCAGAGGGGA CTGTGGGAGC CCAGCCGTAG GCATCATCTT CTTCACCACC TACATCATCA TCTCCTTCCT CATCATGGTC AACATGTACA TTGCAGTGAT TCTGGAGAAC TTCAATGTGG CCACGGAGGA GAGCACTGAG CCCCTGAGTG AGGACGACTT TGACATGTTC TATGAGACCT GGGAGAAGTT TGACCCAGAG GCCACTCAGT TTATTACCTT TTCTGCTCTC TCGGACTTTG CAGACACTCT CTCTGGTCCC CTGAGAATCC CAAAACCCAA TCGAAATATA CTGATCCAGA TGGACCTGCC TTTGGTCCCT GGAGATAAGA TCCACTGCTT GGACATCCTT TTTGCTTTCA CCAAGAATGT CCTAGGAGAA TCCGGGGAGT TGGATTCTCT GAAGGCAAAT ATGGAGGAGA AGTTTATGGC AACTAATCTT TCAAAATCAT CCTATGAACC AATAGCAACC ACTCTCCGAT GGAAGCAAGA AGACATTTCA GCCACTGTCA TTCAAAAGGC CTATCGGAGC TATGTGCTGC ACCGCTCCAT GGCACTCTCT AACACCCCAT GTGTGCCCAG AGCTGAGGAG GAGGCTGCAT CACTCCCAGA TGAAGGTTTT GTTGCATTCA CAGCAAATGA AAATTGTGTA CTCCCAGACA AATCTGAAAC TGCTTCTGCC ACATCATTCC CACCGTCCTA TGAGAGTGTC ACTAGAGGCC TTAGTGATAG AGTCAACATG AGGACATCTA GCTCAATACA AAATGAAGAT GAAGCCACCA GTATGGAGCT GATTGCCCCT GGGCCCCTCG AGGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTA GTGAGGGCCC GATATCTCGA CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAAACGG GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC CTGCCATAGC AGATCTGCGC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGCATCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGGGGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA TGGTCGACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC AACAAGGCAA GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGG
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