NM_002211.4;CD29; FNRB; GPIIA; MDF2; MSK12; VLA-BETA;VLAB
100μg liquid plasmid(Instantly):$105
| name | pRV-MMLV-ITGB1(human)-Puro | another name | apRV-MMLV-ITGB1(human)-Puro |
| Insert Size (bp) | 2397bp | Plasmid host | Mammary cell,retrovirus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | Replicon | pUC | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT ATTCCCAATA AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG GGTCTCCTCA GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA GATTTGGAGA CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC GGTCGTTTCG TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGTTTGCGC CTGCGTCTGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCTGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT TGTGGCCCGA CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT CTGGTAGGAG ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGAACCGAA GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC TGTCTGACTG TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG CCCTCACTCC TTCTCTAGGC GCCGGAATTA GATCTCTCGA GCCACCATGA ATTTACAACC AATTTTCTGG ATTGGACTGA TCAGTTCAGT TTGCTGTGTG TTTGCTCAAA CAGATGAAAA TAGATGTTTA AAAGCAAATG CCAAATCATG TGGAGAATGT ATACAAGCAG GGCCAAATTG TGGGTGGTGC ACAAATTCAA CATTTTTACA GGAAGGAATG CCTACTTCTG CACGATGTGA TGATTTAGAA GCCTTAAAAA AGAAGGGTTG CCCTCCAGAT GACATAGAAA ATCCCAGAGG CTCCAAAGAT ATAAAGAAAA ATAAAAATGT AACCAACCGT AGCAAAGGAA CAGCAGAGAA GCTCAAGCCA GAGGATATTA CTCAGATCCA ACCACAGCAG TTGGTTTTGC GATTAAGATC AGGGGAGCCA CAGACATTTA CATTAAAATT CAAGAGAGCT GAAGACTATC CCATTGACCT CTACTACCTT ATGGACCTGT CTTACTCAAT GAAAGACGAT TTGGAGAATG TAAAAAGTCT TGGAACAGAT CTGATGAATG AAATGAGGAG GATTACTTCG GACTTCAGAA TTGGATTTGG CTCATTTGTG GAAAAGACTG TGATGCCTTA CATTAGCACA ACACCAGCTA AGCTCAGGAA CCCTTGCACA AGTGAACAGA ACTGCACCAG CCCATTTAGC TACAAAAATG TGCTCAGTCT TACTAATAAA GGAGAAGTAT TTAATGAACT TGTTGGAAAA CAGCGCATAT CTGGAAATTT GGATTCTCCA GAAGGTGGTT TCGATGCCAT CATGCAAGTT GCAGTTTGTG GATCACTGAT TGGCTGGAGG AATGTTACAC GGCTGCTGGT GTTTTCCACA GATGCCGGGT TTCACTTTGC TGGAGATGGG AAACTTGGTG GCATTGTTTT ACCAAATGAT GGACAATGTC ACCTGGAAAA TAATATGTAC ACAATGAGCC ATTATTATGA TTATCCTTCT ATTGCTCACC TTGTCCAGAA ACTGAGTGAA AATAATATTC AGACAATTTT TGCAGTTACT GAAGAATTTC AGCCTGTTTA CAAGGAGCTG AAAAACTTGA TCCCTAAGTC AGCAGTAGGA ACATTATCTG CAAATTCTAG CAATGTAATT CAGTTGATCA TTGATGCATA CAATTCCCTT TCCTCAGAAG TCATTTTGGA AAACGGCAAA TTGTCAGAAG GCGTAACAAT AAGTTACAAA TCTTACTGCA AGAACGGGGT GAATGGAACA GGGGAAAATG GAAGAAAATG TTCCAATATT TCCATTGGAG ATGAGGTTCA ATTTGAAATT AGCATAACTT CAAATAAGTG TCCAAAAAAG GATTCTGACA GCTTTAAAAT TAGGCCTCTG GGCTTTACGG AGGAAGTAGA GGTTATTCTT CAGTACATCT GTGAATGTGA ATGCCAAAGC GAAGGCATCC CTGAAAGTCC CAAGTGTCAT GAAGGAAATG GGACATTTGA GTGTGGCGCG TGCAGGTGCA ATGAAGGGCG TGTTGGTAGA CATTGTGAAT GCAGCACAGA TGAAGTTAAC AGTGAAGACA TGGATGCTTA CTGCAGGAAA GAAAACAGTT CAGAAATCTG CAGTAACAAT GGAGAGTGCG TCTGCGGACA GTGTGTTTGT AGGAAGAGGG ATAATACAAA TGAAATTTAT TCTGGCAAAT TCTGCGAGTG TGATAATTTC AACTGTGATA GATCCAATGG CTTAATTTGT GGAGGAAATG GTGTTTGCAA GTGTCGTGTG TGTGAGTGCA ACCCCAACTA CACTGGCAGT GCATGTGACT GTTCTTTGGA TACTAGTACT TGTGAAGCCA GCAACGGACA GATCTGCAAT GGCCGGGGCA TCTGCGAGTG TGGTGTCTGT AAGTGTACAG ATCCGAAGTT TCAAGGGCAA ACGTGTGAGA TGTGTCAGAC CTGCCTTGGT GTCTGTGCTG AGCATAAAGA ATGTGTTCAG TGCAGAGCCT TCAATAAAGG AGAAAAGAAA GACACATGCA CACAGGAATG TTCCTATTTT AACATTACCA AGGTAGAAAG TCGGGACAAA TTACCCCAGC CGGTCCAACC TGATCCTGTG TCCCATTGTA AGGAGAAGGA TGTTGACGAC TGTTGGTTCT ATTTTACGTA TTCAGTGAAT GGGAACAACG AGGTCATGGT TCATGTTGTG GAGAATCCAG AGTGTCCCAC TGGTCCAGAC ATCATTCCAA TTGTAGCTGG TGTGGTTGCT GGAATTGTTC TTATTGGCCT TGCATTACTG CTGATATGGA AGCTTTTAAT GATAATTCAT GACAGAAGGG AGTTTGCTAA ATTTGAAAAG GAGAAAATGA ATGCCAAATG GGACACGGGT GAAAATCCTA TTTATAAGAG TGCCGTAACA ACTGTGGTCA ATCCGAAGTA TGAGGGAAAA TGAGAATTCT ACCGGGTAGG GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCCACA TCCACCGGTA GGCGCCAACC GGCTCCGTTC TTTGGTGGCC CCTTCGCGCC ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG GACGTGACAA ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA GCACCGCTGA GCAATGGAAG CGGGTAGGCC TTTGGGGCAG CGGCCAATAG CAGCTTTGCT CCTTCGCTTT CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG CTCAGGGGCG GGCTCAGGGG CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG CCCGGCATTC TGCACGCTTC AAAAGCGCAC GTCTGCCGCG CTGTTCTCCT CTTCCTCATC TCCGGGCCTT TCGACCTGCA GCCCAAGCTT ACCATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG ATAAAATAAA AGATTTTATT TAGTCTCCAG AAAAAGGGGG GAATGAAAGA CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA TTTTGCAAGG CATGGAAAAT ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTTAGGAA CAGAGAGACA GCAGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG GTCCCCAGAT GCGGTCCCGC CCTCAGCAGT TTCTAGAGAA CCATCAGATG TTTCCAGGGT GCCCCAAGGA CCTGAAATGA CCCTGTGCCT TATTTGAACT AACCAATCAG TTCGCTTCTC GCTTCTGTTC GCGCGCTTCT GCTCCCCGAG CTCAATAAAA GAGCCCACAA CCCCTCACTC GGCGCGCCAG TCCTCCGATA GACTGCGTCG CCCGGGTACC CGTGTATCCA ATAAACCCTC TTGCAGTTGC ATCCGACTTG TGGTCTCGCT GTTCCTTGGG AGGGTCTCCT CTGAGTGATT GACTACCCGT CAGCGGGGGT CTTTCATGGG TAACAGTTTC TTGAAGTTGG AGAACAACAT TCTGAGGGTA GGAGTCGAAT ATTAAGTAAT CCTGACTCAA TTAGCCACTG TTTTGAATCC ACATACTCCA ATACTCCTGA AATAGTTCAT TATGGACAGC GCAGAAAGAG CTGGGGAGAA TTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGCG ATTAGTCCAA TTTGTTAAAG ACAGGATATC AGTGGTCCAG GCTCTAGTTT TGACTCAACA ATATCACCAG CTGAAGCCTA TAGAGTACGA GCCATAGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA AAGGGGGGAA
