NM_207655.2;9030024J15Rik; Erbb; Errb1; Errp; wa-2;wa2; Wa5
100μg liquid plasmid(Instantly):$120
| name | pLV3-CMV-3×HA-Egfr(mouse)-CopGFP | another name | apLV3-CMV-3×HA-Egfr(mouse)-CopGFP |
| Insert Size (bp) | 3633bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTTAACTTT TAAAAGAAAA GGGGGGATTG GGGGGTACAG TGCAGGGGAA AGAATAGTAG ACATAATAGC AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA ATTTTATCGA TACTAGTATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC TCCATAGAAG ATTCTAGAGC CACCATGTAC CCATACGACG TACCTGATTA TGCAACTTAT CCTTATGATG TACCTGATTA TGCTACCTAT CCTTATGATG TCCCAGACTA CGCAGCTAGC CGACCCTCAG GGACCGCGAG AACCACACTG CTGGTGTTGC TGACCGCGCT CTGCGCCGCA GGTGGGGCGT TGGAGGAAAA GAAAGTCTGC CAAGGCACAA GTAACAGGCT CACCCAACTG GGCACTTTTG AAGACCACTT TCTGAGCCTG CAGAGGATGT ACAACAACTG TGAAGTGGTC CTTGGGAACT TGGAAATTAC CTATGTGCAA AGGAATTACG ACCTTTCCTT CTTAAAGACC ATCCAGGAGG TGGCCGGCTA TGTCCTCATT GCCCTCAACA CCGTGGAGAG AATCCCTTTG GAGAACCTGC AGATCATCAG GGGAAATGCT CTTTATGAAA ACACCTATGC CTTAGCCATC CTGTCCAACT ATGGGACAAA CAGAACTGGG CTTAGGGAAC TGCCCATGCG GAACTTACAG GAAATCCTGA TTGGTGCTGT GCGATTCAGC AACAACCCCA TCCTCTGCAA TATGGATACT ATCCAGTGGA GGGACATCGT CCAAAACGTC TTTATGAGCA ACATGTCAAT GGACTTACAG AGCCATCCGA GCAGTTGCCC CAAATGTGAT CCAAGCTGTC CCAATGGAAG CTGCTGGGGA GGAGGAGAGG AGAACTGCCA GAAATTGACC AAAATCATCT GTGCCCAGCA ATGTTCCCAT CGCTGTCGTG GCAGGTCCCC CAGTGACTGC TGCCACAACC AATGTGCTGC GGGGTGTACA GGGCCCCGAG AGAGTGACTG TCTGGTCTGC CAAAAGTTCC AAGATGAGGC CACATGCAAA GACACCTGCC CACCACTCAT GCTGTACAAC CCCACCACCT ATCAGATGGA TGTCAACCCT GAAGGGAAGT ACAGCTTTGG TGCCACCTGT GTGAAGAAGT GCCCCCGAAA CTACGTGGTG ACAGATCATG GCTCATGTGT CCGAGCCTGT GGGCCTGACT ACTACGAAGT GGAAGAAGAT GGCATCCGCA AGTGTAAAAA ATGTGATGGG CCCTGTCGCA AAGTTTGTAA TGGCATAGGC ATTGGTGAAT TTAAAGACAC ACTCTCCATA AATGCTACAA ACATCAAACA CTTCAAATAC TGCACTGCCA TCAGCGGGGA CCTTCACATC CTGCCAGTGG CCTTTAAGGG GGATTCTTTC ACGCGCACTC CTCCTCTAGA CCCACGAGAA CTAGAAATTC TAAAAACCGT AAAGGAAATA ACAGGCTTTT TGCTGATTCA GGCTTGGCCT GATAACTGGA CTGACCTCCA TGCTTTCGAG AACCTAGAAA TAATACGTGG CAGAACAAAG CAACATGGTC AGTTTTCTTT GGCGGTCGTT GGCCTGAACA TCACATCACT GGGGCTGCGT TCCCTCAAGG AGATCAGTGA TGGGGATGTG ATCATTTCTG GAAACCGAAA TTTGTGCTAC GCAAACACAA TAAACTGGAA AAAACTCTTC GGGACACCCA ATCAGAAAAC CAAAATCATG AACAACAGAG CTGAGAAAGA CTGCAAGGCC GTGAACCACG TCTGCAATCC TTTATGCTCC TCGGAAGGCT GCTGGGGCCC TGAGCCCAGG GACTGTGTCT CCTGCCAGAA TGTGAGCAGA GGCAGGGAGT GCGTGGAGAA ATGCAACATC CTGGAGGGGG AACCAAGGGA GTTTGTGGAA AATTCTGAAT GCATCCAGTG CCATCCAGAA TGTCTGCCCC AGGCCATGAA CATCACCTGT ACAGGCAGGG GACCAGACAA CTGCATCCAG TGTGCCCACT ACATTGATGG CCCACACTGT GTCAAGACCT GCCCAGCTGG CATCATGGGA GAGAACAACA CTCTGGTCTG GAAGTATGCA GATGCCAATA ATGTCTGCCA CCTATGCCAC GCCAACTGTA CCTATGGATG TGCTGGGCCA GGTCTTCAAG GATGTGAAGT GTGGCCATCT GGGCCAAAGA TACCATCTAT TGCCACTGGG ATTGTGGGTG GCCTCCTCTT CATAGTGGTG GTGGCCCTTG GGATTGGCCT ATTCATGCGA AGACGTCACA TTGTTCGAAA GCGTACACTA CGCCGCCTGC TTCAAGAGAG AGAGCTCGTG GAACCTCTCA CACCCAGCGG AGAAGCTCCA AACCAAGCCC ACTTGAGGAT ATTAAAGGAA ACAGAATTCA AAAAGATCAA AGTTCTGGGT TCGGGAGCAT TTGGCACAGT GTATAAGGGT CTCTGGATCC CAGAAGGTGA GAAAGTAAAA ATCCCGGTGG CCATCAAGGA GTTAAGAGAA GCCACATCTC CAAAAGCCAA CAAAGAAATC CTTGACGAAG CCTATGTGAT GGCTAGTGTG GACAACCCTC ATGTATGCCG CCTCCTGGGC ATCTGTCTGA CCTCCACTGT CCAGCTCATT ACACAGCTCA TGCCCTACGG TTGCCTCCTG GACTACGTCC GAGAACACAA GGACAACATT GGCTCCCAGT ACCTCCTCAA CTGGTGTGTG CAGATTGCAA AGGGCATGAA CTACCTGGAA GATCGGCGTT TGGTGCACCG TGACTTGGCA GCCAGGAATG TACTGGTGAA GACACCACAG CATGTCAAGA TCACAGATTT TGGGCTGGCC AAACTGCTTG GTGCTGAAGA GAAAGAATAT CATGCCGAGG GGGGCAAAGT GCCTATCAAG TGGATGGCTT TGGAATCAAT TTTACACCGA ATTTATACAC ACCAAAGTGA TGTCTGGAGC TATGGTGTCA CTGTGTGGGA ACTGATGACC TTTGGGTCCA AGCCTTATGA TGGAATCCCA GCAAGTGACA TCTCATCCAT CCTAGAGAAA GGAGAGCGCC TTCCACAGCC ACCTATCTGC ACCATCGATG TCTACATGAT CATGGTCAAG TGCTGGATGA TAGATGCTGA TAGCCGCCCA AAGTTCCGAG AGTTGATTCT TGAATTCTCC AAAATGGCCC GAGACCCACA GCGCTACCTT GTTATCCAGG GGGATGAAAG AATGCATTTG CCAAGCCCTA CAGACTCCAA CTTTTACCGA GCCCTGATGG ATGAAGAGGA CATGGAGGAT GTAGTTGATG CTGATGAGTA TCTTATCCCA CAGCAAGGCT TCTTCAACAG CCCGTCCACG TCGAGGACTC CCCTCTTGAG TTCTCTGAGT GCAACTAGCA ACAATTCCAC TGTGGCTTGC ATTAATAGAA ATGGGAGCTG CCGTGTCAAA GAAGACGCCT TCTTGCAGCG GTACAGCTCC GACCCCACAG GTGCTGTAAC AGAGGACAAC ATAGATGACG CATTCCTCCC TGTACCTGAA TATGTAAACC AATCTGTTCC CAAGAGGCCA GCAGGCTCTG TGCAGAACCC TGTCTATCAC AATCAGCCCC TGCATCCAGC TCCTGGAAGA GACCTGCATT ATCAAAATCC CCACAGCAAT GCAGTGGGCA ACCCTGAGTA TCTCAACACT GCCCAGCCTA CCTGTCTCAG TAGTGGGTTT AACAGCCCTG CACTCTGGAT CCAGAAAGGC AGTCACCAAA TGAGCCTAGA CAACCCTGAC TACCAGCAGG ACTTCTTCCC CAAGGAAACC AAGCCAAATG GCATATTTAA GGGCCCCACA GCTGAAAATG CAGAGTACCT ACGGGTGGCA CCTCCAAGCA GTGAGTTTAT TGGAGCATGA GCGGCCGCGA AGGATCTGCG ATCGCTCCGG TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT CGGCAATTGA ACGGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGCTGAAGC TTCGAGGGGC TCGCATCTCT CCTTCACGCG CCCGCCGCCC TACCTGAGGC CGCCATCCAC GCCGGTTGAG TCGCGTTCTG CCGCCTCCCG CCTGTGGTGC CTCCTGAACT GCGTCCGCCG TCTAGGTAAG TTTAAAGCTC AGGTCGAGAC CGGGCCTTTG TCCGGCGCTC CCTTGGAGCC TACCTAGACT CAGCCGGCTC TCCACGCTTT GCCTGACCCT GCTTGCTCAA CTCTACGTCT TTGTTTCGTT TTCTGTTCTG CGCCGTTACA GATCCAAGCT GTGACCGGCG CCTACGCTAG ACGCCACCAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCAAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGGTG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGTGCTGG TGGGCAGCTT CGCCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TTCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGCTGC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCCATCGCCT TCGCCAGATC CCGCGCTCAG TCGTCCAATT CTGCCGTGGA CGGCACCGCC GGACCCGGCT CCACCGGATC TCGCTAAGTC GACAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCCTGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG AAAATAAGAT CTGCTTTTTG CTTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TAGTAGTTCA TGTCATCTTA TTATTCAGTA TTTATAACTT GCAAAGAAAT GAATATCAGA GAGTGAGAGG AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGCTCTAGCT ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGA CTTTTGCAGA GACGGCCCAA ATTCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GCCAAGCTG
