mCherry-MS2
$70.00
In stock
SKU
PL42790
100μg liquid plasmid(Instantly):$91
| name | mCherry-MS2 | another name | amCherry-MS2 |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | Red | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCTAGAGTCG ACCTGCAGCC CAAGCTTGGG CTGAACATCG AGCGCCAGGG CTCCGTAAAG CTACTAGAGC ACAGGCGGTG CCCCAACGTC CTGGGGCCTC TCCACTAATA ACGGCTACTT CCAATTGATT GGACGCGCCA TCTTGCCTGC CTTATGCATA TTCAGCGGTG AACTGAATAT TCATGAACGA GGCCCGTCCC GTCCCTCCCT CCCTTCCCCC CCCCCCGGAA CCCGCTCCGG AGGACCCGAA GGGCCCCGCC TTCATTACCG ATGCGTAGGA CAAACCATTT TCCCGATGTG TGTGGGGGGA TACTAATGAG AGACTTTAGC TGAAAATGAG CCTGAACTCC GAAGCTGAGT AAAAATGGCC TAACTTTATC CTCCGTTCTG TAAGTCCTCG GTTTGAGTGC ACGGGAAACC CGAAAGGAGG ACGACAGGAC CAGGAACATT CTCCTCCTCC TGTCGCGTCA GAAAGAACAC CCAACCAGGG AGCCGGAGCC CTAGCGTCAA CAACTCCGCC GCGCGCGCTC CGTGTAGGCC GGTGCGGGCG GCCCCGTAGC GCAAGGGAGG GCGGGAAAGG AAGGGGCGGG ACACAAGGGC GAATCTATAA AGGGCGTCAC TCAGCCAGTT CTCTCCTCAG AAGCGCCGAG AGCGCGACCG GGACGGTTGG AGAAGAAGGT GGCTCCCGGA AGGGGGAGAG ACAAACTGCC GTAACCTCTG CCGTTCAGGA TCACTAGTCC ACCATGGGCT ACCCCTACGA CGTGCCCGAC TACGCCATCG AAGGCCGCCA TATGCTAGCC GTTAAAATGG CTTCTAACTT TACTCAGTTC GTTCTCGTCG ACAATGGCGG AACTGGCGAC GTGACTGTCG CCCCAAGCAA CTTCGCTAAC GGGATCGCTG AATGGATCAG CTCTAACTCG CGTTCACAGG CTTACAAAGT AACCTGTAGC GTTCGTCAGA GCTCTGCGCA GAATCGCAAA TACACCATCA AAGTCGAGGT GCCTAAAGGC GCCTGGCGTT CGTACTTAAA TATGGAACTA ACCATTCCAA TTTTCGCCAC GAATTCCGAC TGCGAGCTTA TTGTTAAGGC AATGCAAGGT CTCCTAAAAG ATGGAAACCC GATTCCCTCA GCAATCGCAG CAAACTCCGG CATCTACGCG GATCCAGCCA CCATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCATCAT CAAGGAGTTC ATGCGCTTCA AGGTGCACAT GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGT GGCCCCCTGC CCTTCGCCTG GGACATCCTG TCCCCTCAGT TCATGTACGG CTCCAAGGCC TACGTGAAGC ACCCCGCCGA CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC GCGTGATGAA CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG ACGGCGAGTT CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC AAGCAGAGGC TGAAGCTGAA GGACGGCGGC CACTACGACG CTGAGGTCAA GACCACCTAC AAGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACAACG TCAACATCAA GTTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG AACGCGCCGA GGGCCGCCAC TCCACCGGCG GCATGGACGA GCTGTACAAG CCAAAAAAGA AGAGAAAGGT ATAAATCGAT TCTGCAGTAG CTCGCCGGCA GCTGACTAGC TAGAGCTGAG AACTTCAGGG TGAGTTTGGG GACCCTTGAT TGTTCTTTCT TTTTCGCTAT TGTAAAATTC ATGTTATATG GAGGGGGCAA AGTTTTCAGG GTGTTGTTTA GAATGGGAAG ATGTCCCTTG TATCACCATG GACCCTCATG ATAATTTTGT TTCTTTCACT TTCTACTCTG TTGACAACCA TTGTCTCCTC TTATTTTCTT TTCATTTTCT GTAACTTTTT CGTTAAACTT TAGCTTGCAT TTGTAACGAA TTTTTAAATT CACTTTTGTT TATTTGTCAG ATTGTAAGTA CTTTCTCTAA TCACTTTTTT TTCAAGGCAA TCAGGGTATA TTATATTGTA CTTCAGCACA GTTTTAGAGA ACAATTGTTA TAATTAAATG ATAAGGTAGA ATATTTCTGC ATATAAATTC TGGCTGGCGT GGAAATATTC TTATTGGTAG AAACAACTAC ACCCTGGTCA TCATCCTGCC TTTCTCTTTA TGGTTACAAT GATATACACT GTTTGAGATG AGGATAAAAT ACTCTGAGTC CAAACCGGGC CCCTCTGCTA ACCATGTTCA TGCCTTCTTC TCTTTCCTAC AGCTCCTGGG CAACGTGCTG GTTGTTGTGC TGTCTCATCA TTTTGGCAAA GAATTCACTC CTCAGGTGCA GGCTGCCTAT CAGAAGGTGG TGGCTGGTGT GGCCAATGCC CTGGCTCACA AATACCACTG AGATCTTTTT CCCTCTGCCA AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC TTCTGGCTAA TAAAGGAAAT TTATTTTCAT TGCAATAGTG TGTTGGAATT TTTTGTGTCT CTCACTCGGA AGGACATATG GGAGGGCAAA TCATTTAAAA CATCAGAATG AGTATTTGGT TTAGAGTTTG GCAACATATG CCCATATGCT GGCTGCCATG AACAAAGGTT GGCTATAAAG AGGTCATCAG TATATGAAAC AGCCCCCTGC TGTCCATTCC TTATTCCATA GAAAAGCCTT GACTTGAGGT TAGATTTTTT TTATATTTTG TTTTGTGTTA TTTTTTTCTT TAACATCCCT AAAATTTTCC TTACATGTTT TACTAGCCAG ATTTTTCCTC CTCTCCTGAC TACTCCCAGT CATAGCTGTC CCTCTTCTCT TATGGAGATC CCTCGAGCGG CCGCGGTACC CAATTCGCCC TATAGTGAGT CGTATTACAA TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTCGA AATTAACCCT CACTAAAGGG AACAAAAGCT GGAGCTCCAC CGCGGTGGCG GCCGC
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