pLV2-TRE3GS-NLS-HCN4(human)-FLAG-NLS-TetOne-Hyg
$99.00
In stock
SKU
PL42747
NM_005477.2;SSS2
100μg liquid plasmid(Instantly):$120
| name | pLV2-TRE3GS-NLS-HCN4(human)-FLAG-NLS-TetOne-Hyg | another name | apLV2-TRE3GS-NLS-HCN4(human)-FLAG-NLS-TetOne-Hyg |
| Insert Size (bp) | 3612bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | TRE3GS | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTACTAAGT TTGTAGTACA TATTTAACAA ATACAATTTC TTTAAAAACT ACTAAGTTTG TAGTACATAT TTAACAAATA CAATTTCTTT AAAATGAAAA TAATTCAGAG GAATCACAGG TTTAGAGTAA ATGAAACCAC TAGGTAATTG GCAGTGGTAA TAGGGTATGG GGTGGGAAGT TTGGGATGAT TTTGGTTAGC TTGAGTTATC CAGTTGATCC AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT GATCTCCCGA TCCGTCGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTCT AGCTAGAGGT CGACGGTAAA ACGCTAGCAA TGTCAAGGCC TCTCACTCTC TGATATTCAT TTCTTTGCAA GTTATAAATA CTGAATAATA AGATGACATG AACTACTACT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGGCA AAAAGCAGCT GCTTATATGC AGGATCTGAG GGCTCGCCAC TCCCCAGTCC CGCCCAGGCC ACGCCTCCCT GGAAAGTCCC CAGCGGAAAG TCCCTTGTAG CAAGCTCGAT ATCAGCAGTT CTTGAAGTAC TCCGGATGCA GCTCTCGGGC CACGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AACACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCTGGTG TTCTCTCCTT TATTGGCCTC TTCTACCTTA TCTGGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGACCCCT GGCCCTGGTG TGTAGTTCTG CTAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCT CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACTGCAGAAT TAATTCCAGG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG CGAAGGCGAA GACGCGGAAG AGGCCGCAGA GCCGGCAGCA GGCCGCGGGA AGGAAGGTCC GCTGGATTGA GGGCCGAAGG GACGTAGCAG AAGGACGTCC CGCGCAGAAT CCAGGTGGCA ACACAGGCGA GCAGCCATGG AAAGGACGTC AGCTTCCCCG ACAACACCAC GGAATTGTCA GTGCCCAACA GCCGAGCCCC TGTCCAGCAG CGGGCAAGGC AGGCGGCGAT GAGTTCCGCC GTGGCAATAG GGAGGGGGAA AGCGAAAGTC CCGGAAAGGA GCTGACAGGT GGTGGCAATG CCCCAACCAG TGGGGGTTGC GTCAGCAAAC ACAGTGCACA CCACGCCACG TTGCCTGACA ACGGGCCACA ACTCCTCATA AAGAGACAGC AACCAGGATT TATACAAGGA GGAGAAAATG AAAGCCATAC GGGAAGCAAT AGCATGATAC AAAGGCATTA AAGCAGCGTA TCCACATAGC GTAAAAGGAG CAACATAGTT AAGAATACCA GTCAATCTTT CACAAATTTT GTAATCCAGA GGTTGATTGT TCCAGACGCG TCTATTCCTT TGCCCTCGGA CGAGTGCTGG GGCGTCGGTT TCCACTATCG GCGAGTACTT CTACACAGCC ATCGGTCCAG ACGGCCGCGC TTCTGCGGGC GATTTGTGTA CGCCCGACAG TCCCGGCTCC GGATCGGACG ATTGCGTCGC ATCGACCCTG CGCCCAAGCT GCATCATCGA AATTGCCGTC AACCAAGCTC TGATAGAGTT GGTCAAGACC AATGCGGAGC ATATACGCCC GGAGCCGCGG CGATCCTGCA AGCTCCGGAT GCCTCCGCTC GAAGTAGCGC GTCTGCTGCT CCATACAAGC CAACCACGGC CTCCAGAAGA AGATGTTGGC GACCTCGTAT TGGGAATCCC CGAACATCGC CTCGCTCCAG TCAATGACCG CTGTTATGCG GCCATTGTCC GTCAGGACAT TGTTGGAGCC GAAATCCGCG TGCACGAGGT GCCGGACTTC GGGGCAGTCC TCGGCCCAAA GCATCAGCTC ATCGAGAGCC TGCGCGACGG ACGCACTGAC GGTGTCGTCC ATCACAGTTT GCCAGTGATA CACATGGGGA TCAGCAATCG CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC TTGCGGTCCG AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC CATGGCCTCC GCGACCGGCT GCAGAACAGC GGGCAGTTCG GTTTCAGGCA GGTCTTGCAA CGTGACACCC TGTGCACGGC GGGAGATGCA ATAGGTCAGG CTCTCGCTGA ATTCCCCAAT GTCAAGCACT TCCGGAATCG GGAGCGCGGC CGATGCAAAG TGCCGATAAA CATAACGATC TTTGTAGAAA CCATCGGCGC AGCTATTTAC CCGCAGGACA TATCCACGCC CTCCTACATC GAAGCTGAAA GCACGAGATT CTTCGCCCTC CGAGAGCTGC ATCAGGTCGG AGACGCTGTC GAACTTTTCG ATCAGAAACT TCTCGACAGA CGTCGCGGTG AGTTCAGGCT TTTTCATCAC GTGCTGATCA GATCCGAAAA TGGATATACA AGCTCCCGGG AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCG CGCGTTTACC CGGGGAGCAT GTCAAGGTCA AAATCGTCAA GAGCGTCAGC AGGCAGCATA TCAAGGTCAA AGTCGTCAAG GGCATCGGCT GGGAGCATGT CTAAGTCAAA ATCGTCAAGG GCGTCGGTCG GCCCGCCGCT TTCGCACTTT AGCTGTTTCT CCAGGCCACA TATGATTAGT TCCAGGCCGA AAAGGAAGGC AGGTTCGGCT CCCTGCCGGT CGAACAGCTC AATTGCTTGT TTCAGAAGTG GGGGCATAGA ATCGGTGGTA GGTGTCTCTC TTTCCTCTTT TGCTACTTGA TGCTCCTGTT CCTCCAATAC GCAGCCCAGT GTAAAGTGGC CCACGGCGGA CAGAGCGTAC AGTGCGTTCT CCAGGGAGAA GCCTTGCTGA CACAGGAACG CGAGCTGATT TTCCAGGGTT TCGTACTGTT TCTCTGTTGG GCGGGTGCCG AGATGCACTT TAGCCCCGTC GCGATGTGAG AGGAGAGCAC AGCGGTATGA CTTGGCGTTG TTCCGCAGAA AGTCTTGCCA TGACTCGCCT TCCAGGGGGC AGGAGTGGGT ATGATGCCTG TCCAGCATCT CGATTGGCAG GGCATCGAGC AGGGCCCGCT TGTTCTTCAC GTGCCAGTAC AGGGTAGGCT GCTCAACTCC CAGCTTTTGA GCGAGTTTCC TTGTCGTCAG GCCTTCGATA CCGACTCCAT TGAGTAATTC CAGAGCAGAG TTTATGACTT TGCTCTTGTC CAGTCTAGAC ATGGTAATTC GATGATCCCC CTGGGGAGAG AGGTCGGTGA TTCGGTCAAC GAGGGAGCCG ACTGCCGACG TGCGCTCCGG AGGCTTGCAG AATGCGGAAC ACCGCGCGGG CAGGAACAGG GCCCACACTA CCGCCCCACA CCCCGCCTCC CGCACCGCCC CTTCCCGGCC GCTGCTCTCG GCGCGCCCTG CTGAGCAGCC GCTATTGGCC ACAGCCCATC GCGGTCGGCG CGCTGCCATT GCTCCCTGGC GCTGTCCGTC TGCGAGGGTA CTAGTGAGAC GTGCGGCTTC CGTTTGTCAC GTCCGGCACG CCGCGAACCG CAAGGAACCT TCCCGACTTA GGGGCGGAGC AGGAAGCGTC GCCGGGGGGC CCACAAGGGT AGCGGCGAAG ATCCGGGTGA CGCTGCGAAC GGACGTGAAG AATGTGCGAG ACCCAGGGTC GGCGCCGCTG CGTTTCCCGG AACCACGCCC AGAGCAGCCG CGTCCCTGCG CAAACCCAGG GCTGCCTTGG AAAAGGCGCA ACCCCAACCC CGTGGAATTA TCACCTCGAG TTTACTCCCT ATCAGTGATA GAGAACGTAT GAAGAGTTTA CTCCCTATCA GTGATAGAGA ACGTATGCAG ACTTTACTCC CTATCAGTGA TAGAGAACGT ATAAGGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGA CCAGTTTACT CCCTATCAGT GATAGAGAAC GTATCTACAG TTTACTCCCT ATCAGTGATA GAGAACGTAT ATCCAGTTTA CTCCCTATCA GTGATAGAGA ACGTATAAGC TTTGCTTATG TAAACCAGGG CGCCTATAAA AGAGTGCTGA TTTTTTGAGT AAACTTCAAT TCCACAACAC TTTTGTCTTA TACCAACTTT CCGTACCACT TCCTACCCTC GTAAAGAATT CCCGTATACA CCGGTGCCGG CGGATCCGCC ACCATGCCTA AGAAAAAGCG GAAAGTGATG GACAAGCTGC CGCCGTCCAT GCGCAAGCGG CTCTACAGCC TCCCGCAGCA GGTGGGGGCC AAGGCGTGGA TCATGGACGA GGAAGAGGAC GCCGAGGAGG AGGGGGCCGG GGGCCGCCAA GACCCCAGCC GCAGGAGCAT CCGGCTGCGG CCACTGCCCT CGCCCTCCCC CTCGGCGGCC GCGGGTGGCA CGGAGTCCCG GAGCTCGGCC CTCGGGGCAG CGGACAGCGA AGGGCCGGCC CGCGGCGCGG GCAAGTCCAG CACGAACGGC GACTGCAGGC GCTTCCGCGG GAGCCTGGCC TCGCTGGGCA GCCGGGGCGG CGGCAGCGGC GGCACGGGGA GCGGCAGCAG TCACGGACAC CTGCATGACT CCGCGGAGGA GCGGCGGCTC ATCGCCGAGG GCGACGCGTC CCCCGGCGAG GACAGGACGC CCCCAGGCCT GGCGGCCGAG CCCGAGCGCC CCGGCGCCTC GGCGCAGCCC GCAGCCTCGC CGCCGCCGCC CCAGCAGCCA CCGCAGCCGG CCTCCGCCTC CTGCGAGCAG CCCTCGGTGG ACACCGCTAT CAAAGTGGAG GGAGGCGCGG CTGCCGGCGA CCAGATCCTC CCGGAGGCCG AGGTGCGCCT GGGCCAGGCC GGCTTCATGC AGCGCCAGTT CGGGGCCATG CTCCAACCCG GGGTCAACAA ATTCTCCCTA AGGATGTTCG GCAGCCAGAA AGCCGTGGAG CGCGAACAGG AGAGGGTCAA GTCGGCCGGA TTTTGGATTA TCCACCCCTA CAGTGACTTC AGATTTTACT GGGACCTGAC CATGCTGCTG CTGATGGTGG GAAACCTGAT TATCATTCCT GTGGGCATCA CCTTCTTCAA GGATGAGAAC ACCACACCCT GGATTGTCTT CAATGTGGTG TCAGACACAT TCTTCCTCAT CGACTTGGTC CTCAACTTCC GCACAGGGAT CGTGGTGGAG GACAACACAG AGATCATCCT GGACCCGCAG CGGATTAAAA TGAAGTACCT GAAAAGCTGG TTCATGGTAG ATTTCATTTC CTCCATCCCC GTGGACTACA TCTTCCTCAT TGTGGAGACA CGCATCGACT CGGAGGTCTA CAAGACTGCC CGGGCCCTGC GCATTGTCCG CTTCACGAAG ATCCTCAGCC TCTTACGCCT GTTACGCCTC TCCCGCCTCA TTCGATATAT TCACCAGTGG GAAGAGATCT TCCACATGAC CTACGACCTG GCCAGCGCCG TGGTGCGCAT CGTGAACCTC ATCGGCATGA TGCTCCTGCT CTGCCACTGG GACGGCTGCC TGCAGTTCCT GGTACCCATG CTACAGGACT TCCCTGACGA CTGCTGGGTG TCCATCAACA ACATGGTGAA CAACTCCTGG GGGAAGCAGT ACTCCTACGC GCTCTTCAAG GCCATGAGCC ACATGCTGTG CATCGGCTAC GGGCGGCAGG CGCCCGTGGG CATGTCCGAC GTCTGGCTCA CCATGCTCAG CATGATCGTG GGTGCCACCT GCTACGCCAT GTTCATTGGC CACGCCACTG CCCTCATCCA GTCCCTGGAC TCCTCCCGGC GCCAGTACCA GGAAAAGTAC AAGCAGGTGG AGCAGTACAT GTCCTTTCAC AAGCTCCCGC CCGACACCCG GCAGCGCATC CACGACTACT ACGAGCACCG CTACCAGGGC AAGATGTTCG ACGAGGAGAG CATCCTGGGC GAGCTAAGCG AGCCCCTGCG GGAGGAGATC ATCAACTTTA ACTGTCGGAA GCTGGTGGCC TCCATGCCAC TGTTTGCCAA TGCGGACCCC AACTTCGTGA CGTCCATGCT GACCAAGCTG CGTTTCGAGG TCTTCCAGCC TGGGGACTAC ATCATCCGGG AAGGCACCAT TGGCAAGAAG ATGTACTTCA TCCAGCATGG CGTGGTCAGC GTGCTCACCA AGGGCAACAA GGAGACCAAG CTGGCCGACG GCTCCTACTT TGGAGAGATC TGCCTGCTGA CCCGGGGCCG GCGCACAGCC AGCGTGAGGG CCGACACCTA CTGCCGCCTC TACTCGCTGA GCGTGGACAA CTTCAATGAG GTGCTGGAGG AGTACCCCAT GATGCGAAGG GCCTTCGAGA CCGTGGCGCT GGACCGCCTG GACCGCATTG GCAAGAAGAA CTCCATCCTC CTCCACAAAG TCCAGCACGA CCTCAACTCC GGCGTCTTCA ACTACCAGGA GAATGAGATC ATCCAGCAGA TTGTGCAGCA TGACCGGGAG ATGGCCCACT GCGCGCACCG CGTCCAGGCT GCTGCCTCTG CCACCCCAAC CCCCACGCCC GTCATCTGGA CCCCGCTGAT CCAGGCACCA CTGCAGGCTG CCGCTGCCAC CACTTCTGTG GCCATAGCCC TCACCCACCA CCCTCGCCTG CCTGCTGCCA TCTTCCGCCC TCCCCCAGGA TCTGGGCTGG GCAACCTCGG TGCCGGGCAG ACGCCAAGGC ACCTGAAACG GCTGCAGTCC CTGATCCCTT CTGCGCTGGG CTCCGCCTCG CCCGCCAGCA GCCCGTCCCA GGTGGACACA CCGTCTTCAT CCTCCTTCCA CATCCAACAG CTGGCTGGAT TCTCTGCCCC CGCTGGACTG AGCCCACTCC TGCCCTCATC CAGCTCCTCC CCACCCCCCG GGGCCTGTGG CTCCCCCTCG GCTCCCACAC CATCAGCTGG CGTAGCCGCC ACCACCATAG CCGGGTTTGG CCACTTCCAC AAGGCGCTGG GTGGCTCCCT GTCCTCCTCC GACTCTCCCC TGCTCACCCC GCTGCAGCCA GGCGCCCGCT CCCCGCAGGC TGCCCAGCCA TCTCCCGCGC CACCCGGGGC CCGGGGAGGC CTGGGACTCC CGGAGCACTT CCTGCCACCC CCACCCTCAT CCAGATCCCC GTCATCTAGC CCCGGGCAGC TGGGCCAGCC TCCCGGGGAG TTGTCCCTAG GTCTGGCCAC TGGCCCACTG AGCACGCCAG AGACACCCCC ACGGCAGCCT GAGCCGCCGT CCCTTGTGGC AGGGGCCTCT GGGGGGGCTT CCCCTGTAGG CTTTACTCCC CGAGGAGGTC TCAGCCCCCC TGGCCACAGC CCAGGCCCCC CAAGAACCTT CCCGAGTGCC CCGCCCCGGG CCTCTGGCTC CCACGGATCC TTGCTCCTGC CACCTGCATC CAGCCCCCCA CCACCCCAGG TCCCCCAGCG CCGGGGCACA CCCCCGCTCA CCCCCGGCCG CCTCACCCAG GACCTCAAGC TCATCTCCGC GTCTCAGCCA GCCCTGCCTC AGGACGGGGC GCAGACTCTC CGCAGAGCCT CCCCGCACTC CTCAGGGGAG TCCATGGCTG CCTTCCCGCT CTTCCCCAGG GCTGGGGGTG GCAGCGGGGG CAGTGGGAGC AGCGGGGGCC TCGGTCCCCC TGGGAGGCCC TATGGTGCCA TCCCCGGCCA GCACGTCACT CTGCCTCGGA AGACATCCTC AGGTTCTTTG CCACCCCCTC TGTCTTTGTT TGGGGCAAGA GCCACCTCTT CTGGGGGGCC CCCTCTGACT GCTGGACCCC AGAGGGAACC TGGGGCCAGG CCTGAGCCAG TGCGCTCCAA ACTGCCATCC AATCTAGACT ACAAGGACGA CGATGACAAG CCCAAGAAAA AACGCAAGGT ATGAGACCAC CTCCCCTGCG AGCTAAGCTG GACAGCCAAT GACGGGTAAG AGAGTGACAT TTTTCACTAA CCTAAGACAG GAGGGCCGTC AGAGCTACTG CCTAATCCAA AGACGGGTAA AAGTGATAAA AATGTATCAC TCCAACCTAA GACAGGCGCA GCTTCCGAGG GATTTGAGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA AAACTAGAAT GCAGTGAAAA AAATGCCTTA TTTGTGAAAT TTGTGATGCT ATTGCCTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAAGTCGAT AAACTGGATC TCTGCTGTCC CTGTAATAAA CCCGAAAATT TTGAATTTTT GTAATTTGTT TTTGTAGTTC TTTAGTTTGT ATGTCTGTTG CTATTATGTC TACTATTCTT TCCCCTGCAC TGTACCCCCC AATCCCCCCT TTTCTTTTAA AGGCGATACC GTCGAGATCC GTTCACTAAT CGAATGGATC TGTCTCTGTC TCTCTCTCCA CCTTCTTCTT CTATTCCTTC GGGCCTGTCG GGTCCCCTCG GGGTTGGGAG GTGGGTCTGA AACGATAATG GTGAATATCC CTGCCTAACT CTATTCACTA TAGAAAGTAC AGCAAAAACT ATTCTTAAAC CTACCAAGCC TCCTACTATC ATTATGAATA ATTTTATATA CCACAGCCAA TTTGTTATGT TAAACCAATT CCACAAACTT GCCCATTTAT CTAATTCCAA TAATTCTTGT TCATTCTTTT CTTGCTGGTT TTGCGATTCT TCAATTAAGG AGTGTATTAA GCTTGTGTAA TTGTTAATTT CTCTGTCCCA CTCCATCCAG GTCGTGTGAT TCCAAATCTG TTCCAGAGAT TTATTACTCC AACTAGCATT CCAAGGCACA GCAGTGGTGC AAATGAGTTT TCCAGAGCAA CCCCAAATCC CCAGGAGCTG TTGATCCTTT AGGTATCTTT CCACAGCCAG GATTCTTGCC TGGAGCTGCT TGATGCCCCA GACTGTGAGT TGCAACAGAT GCTGTTGCGC CTCAATAGCC CTCAGCAAAT TGTTCTGCTG CTGCACTATA CCAGACAATA ATTGTCTGGC CTGTACCGTC AGCGTCATTG ACGCTGCGCC CATAGTGCTT CCTGCTGCTC CCAAGAACCC AAGGAACAAA GCTCCTATTC CCACTGCTCT TTTTTCTCTC TGCACCACTC TTCTCTTTGC CTTGGTGGGT GCTACTCCTA ATGGTTCAAT TTTTACTACT TTATATTTAT ATAATTCACT TCTCCAATTG TCCCTCATAT CTCCTCCTCC AGGTCTGAAG ATCAGCGGCC GGCCGCTTGC TGTGCGGTGG TCTTACTTTT GTTTTGCTCT TCCTCTATCT TGTCTAAAGC TTCCTTGGTG TCTTTTATCT CTATCCTTTG ATGCACACAA TAGAGGGTTG CTACTGTATT ATATAATGAT CTAAGTTCTT CTGATCCTGT CTGAAGGGAT GGTTGTAGCT GTCCCAGTAT TTGTCTACAG CCTTCTGATG TTTCTAACAG GCCAGGATTA ACTGCGAATC GTTCTAGCTC CCTGCTTGCC CATACTATAT GTTTTAATTT ATATTTTTTC TTTCCCCCTG GCCTTAACCG AATTTTTTCC CATCGCGATC TAATTCTCCC CCGCTTAATA CTGACGCTCT CGCACCCATC TCTCTCCTTC TAGCCTCCGC TAGTCAAAAT TTTTGGCGTA CTCACCAGTC GCCGCCCCTC GCCTCTTGCC GTGCGCGCTT CAGCAAGCCG AGTCCTGCGT CGAGAGAGCT CCTCTGGTTT CCCTTTCGCT TTCAAGTCCC TGTTCGGGCG CCACTGCTAG AGATTTTCCA CACTGACTAA AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGGCAAAAAG CAGCTGCTTA TATGCAGGAT CTGAGGGCTC GCCACTCCCC AGTCCCGCCC AGGCCACGCC TCCCTGGAAA GTCCCCAGCG GAAAGTCCCT TGTAGCAAGC TCGATATCAG CAGTTCTTGA AGTACTCCGG ATGCAGCTCT CGGGCCACGT GATGAAATGC TAGGCGGCTG TCAAACCTCC ACTCTAACAC TTCTCTCTCC GGGTCATCCA TCCCATGCAG GCTCACAGGG TGTAACAAGC TGGTGTTCTC TCCTTTATTG GCCTCTTCTA CCTTATCTGG CTCAACTGGT ACTAGCTTGT AGCACCATCC AAAGGTCAGT GGATATCTGA CCCCTGGCCC TGGTGTGTAG TTCTGCTAAT CAGGGAAGTA GCCTTGTGTG TGGTAGATCC ACAGATCAAG GATATCTTGT CTTCTTTGGG AGTGAATTAG CCCTTCCA
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