pCMV-GLI2(human)-3×HA-Puro
$113.00
In stock
SKU
PL42560
NM_001371271.1;CJS; HPE9; PHS2; THP1; THP2
100μg liquid plasmid(Instantly):$134
| name | pCMV-GLI2(human)-3×HA-Puro | another name | apCMV-GLI2(human)-3×HA-Puro |
| Insert Size (bp) | 4761bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC CACTAGTCCA GTGTGGTGGA ATTCGCCACC ATGGAGACGT CTGCCTCAGC CACTGCCTCC GAGAAGCAAG AAGCCAAAAG TGGGATCCTG GAGGCCGCTG GCTTCCCCGA CCCGGGTAAA AAGGCCTCTC CTTTGGTGGT GGCTGCAGCG GCAGCAGCAG CGGTAGCTGC CCAAGGAGTG CCGCAGCATC TCTTGCCACC ATTCCATGCG CCCCTACCGA TTGACATGCG ACACCAGGAA GGAAGGTACC ATTACGAGCC TCATTCTGTC CACGGTGTGC ACGGGCCCCC TGCCCTCAGC GGCAGCCCTG TCATCTCTGA CATCTCCTTG ATCCGGCTTT CCCCGCACCC GGCTGGCCCT GGGGAGTCCC CCTTCAACGC CCCCCACCCG TACGTGAACC CCCACATGGA GCACTACCTC CGTTCTGTGC ACAGCAGCCC CACGCTCTCC ATGATCTCTG CAGCCAGGGG CCTCAGCCCC GCTGATGTGG CCCAGGAGCA CCTTAAGGAG AGGGGACTGT TTGGCCTTCC TGCTCCAGGC ACCACCCCCT CAGACTATTA CCACCAGATG ACCCTCGTGG CAGGCCACCC CGCGCCCTAC GGGGACCTGC TGATGCAGAG CGGGGGCGCT GCCAGCGCAC CCCATCTCCA CGACTACCTC AACCCCGTGG ACGTGTCCCG TTTCTCCAGC CCGCGGGTGA CGCCCCGCCT GAGCCGCAAG CGGGCGCTGT CCATCTCCCC ACTCTCAGAC GCCAGCCTGG ACCTGCAGCG GATGATCCGC ACCTCACCCA ACTCGCTAGT GGCCTACATC AACAACTCCC GAAGCAGCTC GGCGGCCAGC GGTTCCTACG GGCATCTGTC AGCGGGTGCC CTCAGCCCAG CCTTCACCTT CCCCCACCCC ATCAACCCCG TGGCCTACCA GCAGATTCTG AGCCAGCAGA GGGGTCTGGG GTCAGCCTTT GGACACACAC CACCCCTGAT CCAGCCCTCA CCCACCTTCC TGGCCCAGCA GCCCATGGCC CTCACCTCCA TCAATGCCAC GCCCACCCAG CTCAGCAGCA GCAGCAACTG TCTGAGTGAC ACCAACCAGA ACAAGCAGAG CAGTGAGTCG GCCGTCAGCA GCACCGTCAA CCCTGTCGCC ATTCACAAGC GCAGCAAGGT CAAGACCGAG CCTGAGGGCC TGCGGCCGGC CTCCCCTCTG GCGCTGACGC AGGGCCAGGT GTCTGGACAC GGCTCATGTG GGTGTGCCCT TCCCCTCTCC CAGGAGCAGC TGGCTGACCT CAAGGAAGAT CTGGACAGGG ATGACTGTAA GCAGGAGGCT GAGGTGGTCA TCTATGAGAC CAACTGCCAC TGGGAAGACT GCACCAAGGA GTACGACACC CAGGAGCAGC TGGTGCATCA CATCAACAAC GAGCACATCC ACGGGGAGAA GAAGGAGTTT GTGTGCCGCT GGCAGGCCTG CACGCGGGAG CAGAAGCCCT TCAAGGCGCA GTACATGCTG GTGGTGCACA TGCGGCGACA CACGGGCGAG AAGCCCCACA AGTGCACGTT CGAGGGCTGC TCGAAGGCCT ACTCCCGCCT GGAGAACCTG AAGACACACC TGCGGTCCCA CACCGGGGAG AAGCCATATG TGTGTGAGCA CGAGGGCTGC AACAAAGCCT TCTCCAACGC CTCGGACCGC GCCAAGCACC AGAATCGCAC CCACTCCAAC GAGAAACCCT ACATCTGCAA GATCCCAGGC TGCACCAAGA GATACACAGA CCCCAGCTCT CTCCGGAAGC ATGTGAAAAC GGTCCACGGC CCAGATGCCC ACGTCACCAA GAAGCAGCGC AATGACGTGC ACCTCCGCAC ACCGCTGCTC AAAGAGAATG GGGACAGTGA GGCCGGCACG GAGCCTGGCG GCCCAGAGAG CACCGAGGCC AGCAGCACCA GCCAGGCCGT GGAGGACTGC CTGCACGTCA GAGCCATCAA GACCGAGAGC TCCGGGCTGT GTCAGTCCAG CCCCGGGGCC CAGTCGTCCT GCAGCAGCGA GCCCTCTCCT CTGGGCAGTG CCCCCAACAA TGACAGTGGC GTGGAGATGC CGGGGACGGG GCCCGGGAGC CTGGGAGACC TGACGGCACT GGATGACACA CCCCCAGGGG CCGACACCTC AGCCCTGGCT GCCCCCTCCG CTGGTGGCCT CCAGCTGCGC AAACACATGA CCACCATGCA CCGGTTCGAG CAGCTCAAGA AGGAGAAGCT CAAGTCACTC AAGGATTCCT GCTCATGGGC CGGGCCGACT CCACACACGC GGAACACCAA GCTGCCTCCC CTCCCGGGAA GTGGCTCCAT CCTGGAAAAC TTCAGTGGCA GTGGGGGCGG CGGGCCCGCG GGGCTGCTGC CGAACCCGCG GCTGTCGGAG CTGTCCGCGA GCGAGGTGAC CATGCTGAGC CAGCTGCAGG AGCGCCGCGA CAGCTCCACC AGCACGGTCA GCTCGGCCTA CACCGTGAGC CGCCGCTCCT CCGGCATCTC CCCCTACTTC TCCAGCCGCC GCTCCAGCGA GGCCTCGCCC CTGGGCGCCG GCCGCCCGCA CAACGCGAGC TCCGCTGACT CCTACGACCC CATCTCCACG GACGCGTCGC GGCGCTCGAG CGAGGCCAGC CAGTGCAGCG GCGGCTCCGG GCTGCTCAAC CTCACGCCGG CGCAGCAGTA CAGCCTGCGG GCCAAGTACG CGGCAGCCAC TGGCGGCCCC CCGCCCACTC CGCTGCCGGG CCTGGAGCGC ATGAGCCTGC GGACCAGGCT GGCGCTGCTG GACGCGCCCG AGCGCACGCT GCCCGCCGGC TGCCCACGCC CACTGGGGCC GCGGCGTGGC AGCGACGGGC CGACCTATGG CCACGGCCAC GCGGGGGCTG CGCCCGCCTT CCCCCACGAG GCTCCAGGCG GCGGAGCCAG GCGGGCCAGC GACCCTGTGC GGCGGCCCGA TGCCCTGTCC CTGCCGCGGG TGCAGCGCTT CCACAGCACC CACAACGTGA ACCCCGGCCC GCTGCCGCCC TGTGCCGACA GGCGAGGCCT CCGCCTGCAG AGCCACCCGA GCACCGACGG CGGCCTGGCC CGCGGCGCCT ACTCGCCCCG GCCGCCTAGC ATCAGCGAGA ACGTGGCGAT GGAGGCCGTG GCGGCAGGAG TGGACGGCGC GGGGCCCGAG GCCGACCTGG GGCTGCCGGA GGACGACCTG GTGCTTCCAG ACGACGTGGT GCAGTACATC AAGGCGCACG CCAGTGGCGC TCTGGACGAG GGCACCGGGC AGGTGTATCC CACGGAAAGC ACTGGCTTCT CTGACAACCC CAGACTACCC AGCCCGGGGC TGCACGGCCA GCGCAGGATG GTGGCTGCGG ACTCCAACGT GGGCCCCTCC GCCCCTATGC TGGGAGGATG CCAGTTAGGC TTTGGGGCGC CCTCCAGCCT GAACAAAAAT AACATGCCTG TGCAGTGGAA TGAGGTGAGC TCCGGCACCG TAGACGCCCT GGCCAGCCAG GTGAAGCCTC CACCCTTTCC TCAGGGCAAC CTGGCGGTGG TGCAGCAGAA GCCTGCCTTT GGCCAGTACC CGGGCTACAG TCCGCAAGGC CTACAGGCTA GCCCTGGGGG CCTGGACAGC ACGCAGCCAC ACCTGCAGCC CCGCAGCGGA GCCCCCTCCC AGGGCATCCC CAGGGTAAAC TACATGCAGC AGCTGCGACA GCCAGTGGCA GGCAGCCAGT GTCCTGGCAT GACTACCACT ATGAGCCCCC ATGCCTGCTA TGGCCAAGTC CACCCCCAGC TGAGCCCCAG CACCATCAGT GGGGCCCTCA ACCAGTTCCC CCAATCCTGC AGCAACATGC CAGCCAAGCC AGGGCATCTG GGGCACCCTC AGCAGACAGA AGTGGCACCT GACCCCACCA CGATGGGCAA TCGCCACAGG GAACTTGGGG TCCCCGATTC AGCCCTGGCT GGAGTGCCAC CACCTCACCC AGTCCAGAGC TACCCACAGC AGAGCCATCA CCTGGCAGCC TCCATGAGCC AGGAGGGCTA CCACCAGGTC CCCAGCCTTC TGCCTGCCCG CCAGCCTGGC TTCATGGAGC CCCAAACAGG CCCGATGGGG GTGGCTACAG CAGGCTTTGG CCTAGTGCAG CCCCGGCCTC CCCTCGAGCC CAGCCCCACT GGCCGCCACC GTGGGGTACG TGCTGTGCAG CAGCAGCTGG CCTACGCCAG GGCCACAGGC CATGCCATGG CTGCCATGCC GTCCAGTCAG GAAACAGCAG AGGCTGTGCC CAAGGGAGCG ATGGGCAACA TGGGGTCGGT GCCTCCCCAG CCGCCTCCGC AGGACGCAGG TGGGGCCCCG GACCACAGCA TGCTCTACTA CTACGGCCAG ATCCACATGT ACGAACAGGA TGGAGGCCTG GAGAACCTCG GGAGCTGCCA GGTCATGCGG TCCCAGCCAC CACAGCCACA GGCCTGTCAG GACAGCATCC AGCCCCAGCC CTTGCCCTCA CCAGGGGTCA ACCAGGTGTC CAGCACTGTG GACTCCCAGC TCCTGGAGGC CCCCCAGATT GACTTCGATG CCATCATGGA TGATGGCGAT CACTCGAGTT TGTTCTCGGG TGCTCTGAGC CCCAGCCTCC TCCACAGCCT CTCCCAGAAC TCCTCCCGCC TCACCACCCC CCGAAACTCC TTGACCCTGC CCTCCATCCC CGCAGGCATC AGCAACATGG CTGTCGGGGA CATGAGCTCC ATGCTCACCA GCCTCGCCGA GGAGAGCAAG TTCCTGAACA TGATGACCCT CGAGTACCCA TACGACGTAC CTGATTATGC AACTTATCCT TATGATGTAC CTGATTATGC TAGCTATCCT TATGATGTCC CAGACTACGC ATAGTCTAGA GGGCCCGTTT AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA CGACGTCCCC CGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG CCACACCGTC GACCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC CCGCAAGCCC GGTGCCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT
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