pT3-EF1a-CTNNB1(human)
$84.00
In stock
SKU
PL42497
NM_001904.4;armadillo; CTNNB; EVR7; MRD19; NEDSDV
100μg liquid plasmid(Instantly):$105
| name | pT3-EF1a-CTNNB1(human) | another name | apT3-EF1a-CTNNB1(human) |
| Insert Size (bp) | 2346bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | EF1a | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT ACTTCCACCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTAGCTT GGTACTAATA CGACTCACTA TAGGGAGACC CAAGCTGGCT AGGTAAGCTT GGTACCGAGC TCGGATCCAC TAGTCCAGTG TGGTGGAATT CTGCAGATAT CAACAAGTTT GTACAAAAAA GCAGGCTCCG CGGCCGCCCC CTTCACCATG GCTACTCAAG CTGATTTGAT GGAGTTGGAC ATGGCCATGG AACCAGACAG AAAAGCGGCT GTTAGTCACT GGCAGCAACA GTCTTACCTG GACTCTGGAA TCCATTCTGG TGCCACTACC ACAGCTCCTT CTCTGAGTGG TAAAGGCAAT CCTGAGGAAG AGGATGTGGA TACCTCCCAA GTCCTGTATG AGTGGGAACA GGGATTTTCT CAGTCCTTCA CTCAAGAACA AGTAGCTGAT ATTGATGGAC AGTATGCAAT GACTCGAGCT CAGAGGGTAC GAGCTGCTAT GTTCCCTGAG ACATTAGATG AGGGCATGCA GATCCCATCT ACACAGTTTG ATGCTGCTCA TCCCACTAAT GTCCAGCGTT TGGCTGAACC ATCACAGATG CTGAAACATG CAGTTGTAAA CTTGATTAAC TATCAAGATG ATGCAGAACT TGCCACACGT GCAATCCCTG AACTGACAAA ACTGCTAAAT GACGAGGACC AGGTGGTGGT TAATAAGGCT GCAGTTATGG TCCATCAGCT TTCTAAAAAG GAAGCTTCCA GACACGCTAT CATGCGTTCT CCTCAGATGG TGTCTGCTAT TGTACGTACC ATGCAGAATA CAAATGATGT AGAAACAGCT CGTTGTACCG CTGGGACCTT GCATAACCTT TCCCATCATC GTGAGGGCTT ACTGGCCATC TTTAAGTCTG GAGGCATTCC TGCCCTGGTG AAAATGCTTG GTTCACCAGT GGATTCTGTG TTGTTTTATG CCATTACAAC TCTCCACAAC CTTTTATTAC ATCAAGAAGG AGCTAAAATG GCAGTGCGTT TAGCTGGTGG GCTGCAGAAA ATGGTTGCCT TGCTCAACAA AACAAATGTT AAATTCTTGG CTATTACGAC AGACTGCCTT CAAATTTTAG CTTATGGCAA CCAAGAAAGC AAGCTCATCA TACTGGCTAG TGGTGGACCC CAAGCTTTAG TAAATATAAT GAGGACCTAT ACTTACGAAA AACTACTGTG GACCACAAGC AGAGTGCTGA AGGTGCTATC TGTCTGCTCT AGTAATAAGC CGGCTATTGT AGAAGCTGGT GGAATGCAAG CTTTAGGACT TCACCTGACA GATCCAAGTC AACGTCTTGT TCAGAACTGT CTTTGGACTC TCAGGAATCT TTCAGATGCT GCAACTAAAC AGGAAGGGAT GGAAGGTCTC CTTGGGACTC TTGTTCAGCT TCTGGGTTCA GATGATATAA ATGTGGTCAC CTGTGCAGCT GGAATTCTTT CTAACCTCAC TTGCAATAAT TATAAGAACA AGATGATGGT CTGCCAAGTG GGTGGTATAG AGGCTCTTGT GCGTACTGTC CTTCGGGCTG GTGACAGGGA AGACATCACT GAGCCTGCCA TCTGTGCTCT TCGTCATCTG ACCAGCCGAC ACCAAGAAGC AGAGATGGCC CAGAATGCAG TTCGCCTTCA CTATGGACTA CCAGTTGTGG TTAAGCTCTT ACACCCACCA TCCCACTGGC CTCTGATAAA GGCTACTGTT GGATTGATTC GAAATCTTGC CCTTTGTCCC GCAAATCATG CACCTTTGCG TGAGCAGGGT GCCATTCCAC GACTAGTTCA GTTGCTTGTT CGTGCACATC AGGATACCCA GCGCCGTACG TCCATGGGTG GGACACAGCA GCAATTTGTG GAGGGGGTCC GCATGGAAGA AATAGTTGAA GGTTGTACCG GAGCCCTTCA CATCCTAGCT CGGGATGTTC ACAACCGAAT TGTTATCAGA GGACTAAATA CCATTCCATT GTTTGTGCAG CTGCTTTATT CTCCCATTGA AAACATCCAA AGAGTAGCTG CAGGGGTCCT CTGTGAACTT GCTCAGGACA AGGAAGCTGC AGAAGCTATT GAAGCTGAGG GAGCCACAGC TCCTCTGACA GAGTTACTTC ACTCTAGGAA TGAAGGTGTG GCGACATATG CAGCTGCTGT TTTGTTCCGA ATGTCTGAGG ACAAGCCACA AGATTACAAG AAACGGCTTT CAGTTGAGCT GACCAGCTCT CTCTTCAGAA CAGAGCCAAT GGCTTGGAAT GAGACTGCTG ATCTTGGACT TGATATTGGT GCCCAGGGAG AACCCCTTGG ATATCGCCAG GATGATCCTA GCTATCGTTC TTTTCACTCT GGTGGATATG GCCAGGATGC CTTGGGTATG GACCCCATGA TGGAACATGA GATGGGTGGC CACCACCCTG GTGCTGACTA TCCAGTTGAT GGGCTGCCAG ATCTGGGGCA TGCCCAGGAC CTCATGGATG GGCTGCCTCC AGGTGACAGC AATCAGCTGG CCTGGTTTGA TACTGACCTG TAATTTAAAC CCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AATAACTTCG TATAATGTAT GCTATACGAA GTTATATCGT ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGAATTC CTTGAAATAC ATCCACAGGT ACACCTCCAA TTGACTCAAA TGATGTCAAT TAGTCTATCA GAAGCTTCTA AAGCCATGAC ATCATTTTCT GGAATTTTCC AAGCTGTTTA AAGGCACAGT CAACTTAGTG TATGTAAACT TCTGACCCAC TGGAATTGTG ATACAGTGAA TTATAAGTGA AATAATCTGT CTGTAAACAA TTGTTGGAAA AATGACTTGT GTCATGCACA AAGTAGATGT CCTAACTGAC TTGCCAAAAC TATTGTTTGT TAACAAGAAA TTTGTGGAGT AGTTGAAAAA CGAGTTTTAA TGACTCCAAC TTAAGTGTAT GTAAACTTCC GACTTCAACT GTAGGGTACC GAGCTCGAAT TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGACTCTAG ACCTATACAG TTGAAGTCGG AAGTTTACAT ACACTTAAGT TGGAGTCATT AAAACTCGTT TTTCAACTAC TCCACAAATT TCTTGTTAAC AAACAATAGT TTTGGCAAGT CAGTTAGGAC ATCTACTTTG TGCATGACAC AAGTCATTTT TCCAACAATT GTTTACAGAC AGATTATTTC ACTTATAATT CACTGTATCA CAATTCCAGT GGGTCAGAAG TTTACATACA CTAAGTTGAC TGTGCCTTTA AACAGCTTGG AAAATTCCAG AAAATGATGT CATGGCTTTA GAAGCTTCTG ATAGACTAAT TGACATCATT TGAGTCAATT GGAGGTGTAC CTGTGGATGT ATTTCAAGGG GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGCGG CCGCTAGCTC GAGATCTTAA TTAAACTAGT ATCGATATAA CTTCGTATAA TGTATGCTAT ACGAAGTTAT ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGGCTTTTG CAAAAAGCTT TGCAAAGATG GATAAAGTTT TAAACAGAGA GGAATCTTTG CAGCTAATGG ACCTTCTAGG TCTTGAAAGG AGTGGGAATT
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