NM_007366.5;CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R
100μg liquid plasmid(Instantly):$134
| name | pLV3-CMV-PLA2R1(human)-P2A-mCherry-Puro | another name | apLV3-CMV-PLA2R1(human)-P2A-mCherry-Puro |
| Insert Size (bp) | 4392bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Red |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCGCGCAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGA GCTGCAAGCT TAATGTAGTC TTATGCAATA CTCTTGTAGT CTTGCAACAT GGTAACGATG AGTTAGCAAC ATGCCTTACA AGGAGAGAAA AAGCACCGTG CATGCCGATT GGTGGAAGTA AGGTGGTACG ATCGTGCCTT ATTAGGAAGG CAACAGACGG GTCTGACATG GATTGGACGA ACCACTGAAT TGCCGCATTG CAGAGATATT GTATTTAAGT GCCTAGCTCG ATACATAAAC GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCGCTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTTGG AATCACACGA CCTGGATGGA GTGGGACAGA GAAATTAACA ATTACACAAG CTTAATACAC TCCTTAATTG AAGAATCGCA AAACCAGCAA GAAAAGAATG AACAAGAATT ATTGGAATTA GATAAATGGG CAAGTTTGTG GAATTGGTTT AACATAACAA ATTGGCTGTG GTATATAAAA TTATTCATAA TGATAGTAGG AGGCTTGGTA GGTTTAAGAA TAGTTTTTGC TGTACTTTCT ATAGTGAATA GAGTTAGGCA GGGATATTCA CCATTATCGT TTCAGACCCA CCTCCCAACC CCGAGGGGAC CCGACAGGCC CGAAGGAATA GAAGAAGAAG GTGGAGAGAG AGACAGAGAC AGATCCATTC GATTAGTGAA CGGATCTCGA CGGTATCGGT TAACTTTTAA AAGAAAAGGG GGGATTGGGG GGTACAGTGC AGGGGAAAGA ATAGTAGACA TAATAGCAAC AGACATACAA ACTAAAGAAT TACAAAAACA AATTACAAAA TTCAAAATTT TATCGATAAG CTTGGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGA CTCTAGACGG GATCCGCCAC CATGCTGCTG TCGCCGTCGC TGCTGCTGCT GCTGCTGCTG GGGGCGCCGC GGGGCTGCGC CGAGGGTGTG GCGGCGGCGC TTACCCCCGA GCGGCTCCTG GAGTGGCAGG ATAAAGGAAT ATTTGTTATC CAAAGTGAGA GTCTCAAGAA ATGCATTCAA GCAGGTAAAT CGGTTCTGAC CCTGGAGAAC TGCAAGCAAG CAAACAAGCA CATGCTGTGG AAATGGGTTT CAAACCATGG CCTCTTTAAC ATAGGAGGCA GTGGTTGCCT GGGCCTGAAT TTCTCCGCCC CAGAGCAGCC ATTAAGCTTA TATGAATGTG ACTCCACCCT CGTTTCCTTA CGGTGGCGCT GTAACAGGAA GATGATCACA GGCCCGCTGC AGTACTCTGT CCAGGTGGCG CATGACAACA CAGTGGTGGC CTCACGGAAG TATATTCATA AGTGGATTTC TTATGGGTCA GGTGGTGGAG ACATTTGTGA ATATCTACAC AAAGATTTGC ATACAATCAA AGGGAACACC CACGGGATGC CGTGTATGTT TCCCTTCCAG TATAACCATC AGTGGCATCA TGAATGTACC CGTGAAGGTC GGGAAGATGA CTTACTGTGG TGTGCCACGA CAAGCCGTTA TGAAAGAGAT GAAAAGTGGG GATTTTGCCC TGATCCCACC TCTGCAGAAG TAGGTTGTGA TACTATTTGG GAGAAGGACC TCAATTCACA CATTTGCTAC CAGTTCAACC TGCTTTCATC TCTCTCTTGG AGTGAGGCAC ATTCTTCATG CCAGATGCAA GGAGGTACGC TGTTAAGTAT TACAGATGAA ACTGAAGAAA ATTTCATAAG GGAGCACATG AGCAGTAAAA CAGTGGAGGT GTGGATGGGC CTCAATCAGC TGGATGAACA CGCTGGCTGG CAGTGGTCTG ATGGAACGCC GCTCAACTAT CTGAATTGGA GCCCAGAGGT AAATTTTGAG CCATTTGTTG AAGATCACTG TGGAACATTT AGTTCATTTA TGCCAAGTGC CTGGAGGAGT CGGGATTGTG AGTCCACCTT GCCATATATA TGTAAAAAAT ATCTAAACCA CATTGATCAT GAAATAGTTG AAAAAGATGC GTGGAAATAT TATGCTACCC ACTGTGAGCC TGGCTGGAAT CCCTACAATC GTAATTGCTA CAAACTTCAG AAAGAAGAAA AGACCTGGCA TGAGGCTCTG CGTTCTTGTC AGGCTGATAA CAGTGCATTA ATAGACATAA CCTCATTAGC AGAGGTGGAG TTTCTTGTAA CCCTCCTTGG AGATGAAAAT GCATCAGAAA CATGGATTGG TTTGAGCAGC AATAAAATTC CAGTTTCCTT TGAATGGTCT AATGACTCTT CAGTCATCTT TACTAATTGG CACACACTTG AGCCCCACAT TTTTCCAAAT AGAAGCCAGC TGTGTGTCTC AGCAGAGCAG TCTGAGGGAC ACTGGAAAGT CAAAAATTGT GAAGAAAGAC TTTTTTACAT TTGTAAAAAA GCAGGCCATG TCCTCTCTGA TGCTGAATCA GGATGTCAAG AGGGATGGGA GAGACATGGT GGATTCTGTT ACAAAATTGA CACAGTCCTT CGAAGCTTTG ACCAAGCTTC CAGCGGTTAT TACTGTCCTC CTGCACTTGT AACCATTACA AACAGGTTTG AACAGGCTTT TATTACCAGT TTGATCAGTA GTGTGGTAAA AATGAAGGAC AGTTATTTTT GGATAGCTCT TCAGGACCAA AATGATACGG GAGAATACAC TTGGAAGCCA GTAGGGCAGA AACCCGAGCC GGTGCAGTAC ACACACTGGA ACACACACCA GCCGCGCTAC AGTGGTGGCT GTGTTGCCAT GCGAGGAAGG CATCCACTTG GTCGCTGGGA AGTGAAGCAC TGTCGGCACT TTAAGGCAAT GTCCTTGTGC AAGCAGCCAG TTGAAAATCA GGAAAAAGCA GAGTATGAAG AGAGATGGCC CTTTCACCCC TGCTATTTGG ACTGGGAGTC AGAGCCTGGT CTGGCCAGTT GCTTCAAGGT ATTTCATAGT GAAAAAGTTC TGATGAAAAG AACATGGAGA GAAGCTGAAG CATTTTGCGA AGAATTTGGA GCTCATCTTG CAAGCTTTGC CCATATTGAG GAAGAGAATT TTGTGAATGA GCTCTTACAT TCAAAATTTA ATTGGACAGA AGAAAGGCAG TTCTGGATTG GATTTAATAA AAGAAACCCA CTGAATGCCG GCTCATGGGA GTGGTCTGAT AGAACTCCTG TTGTCTCTTC GTTTTTAGAC AACACTTATT TTGGAGAAGA TGCAAGAAAC TGTGCTGTTT ATAAGGCAAA CAAAACATTG CTGCCCTTAC ACTGTGGTTC CAAACGTGAA TGGATATGCA AAATCCCAAG AGATGTGAAA CCCAAGATTC CGTTCTGGTA CCAGTACGAT GTACCCTGGC TCTTTTATCA GGATGCAGAA TACCTTTTTC ATACCTTTGC CTCAGAATGG TTGAACTTTG AGTTTGTCTG TAGCTGGCTG CACAGTGATC TTCTCACAAT TCATTCTGCA CATGAGCAAG AATTCATCCA CAGCAAAATA AAAGCGCTAT CAAAGTATGG TGCAAGTTGG TGGATTGGAC TTCAAGAAGA AAGAGCCAAT GATGAATTTC GCTGGAGAGA TGGAACACCA GTGATATACC AGAACTGGGA CACAGGAAGA GAAAGAACTG TGAATAATCA GAGCCAGAGA TGTGGCTTTA TTTCTTCTAT AACAGGACTC TGGGGTAGTG AAGAGTGTTC AGTTTCTATG CCTAGTATCT GTAAGCGAAA AAAGGTTTGG CTCATAGAGA AAAAGAAAGA TACACCAAAA CAACATGGAA CGTGTCCCAA AGGATGGCTA TATTTTAACT ATAAGTGCCT TCTGCTGAAT ATCCCCAAAG ACCCAAGCAG TTGGAAGAAC TGGACGCATG CTCAACATTT CTGTGCTGAA GAAGGGGGGA CCCTGGTCGC CATTGAAAGT GAGGTGGAGC AAGCTTTCAT TACTATGAAT CTTTTTGGCC AGACCACCAG TGTGTGGATA GGTTTACAAA ATGATGATTA TGAAACATGG CTAAATGGAA AGCCTGTGGT ATATTCTAAC TGGTCTCCAT TTGATATAAT AAATATTCCA AGTCACAATA CCACTGAAGT TCAGAAACAC ATTCCTCTCT GTGCCTTACT CTCAAGTAAT CCTAATTTTC ATTTCACTGG AAAATGGTAT TTTGAAGACT GTGGAAAGGA AGGCTATGGG TTTGTTTGTG AAAAAATGCA AGATACTTCT GGACACGGTG TAAATACATC TGATATGTAT CCAATGCCCA ATACCTTAGA ATATGGAAAC AGAACTTACA AAATAATTAA TGCAAATATG ACTTGGTATG CAGCAATAAA AACCTGCCTG ATGCACAAAG CACAACTGGT CAGCATCACA GACCAGTATC ACCAGTCCTT CCTCACTGTT GTCCTCAACC GGCTAGGATA TGCCCACTGG ATTGGACTGT TCACCACAGA TAATGGTCTT AATTTTGACT GGTCTGATGG CACCAAATCT TCTTTCACTT TTTGGAAAGA TGAGGAGTCC TCCCTCCTTG GTGACTGCGT TTTTGCCGAC AGCAACGGAC GCTGGCATAG CACAGCCTGC GAGTCATTTC TGCAAGGTGC CATTTGTCAT GTGCCACCTG AAACAAGACA ATCTGAACAC CCAGAGTTGT GCTCAGAAAC ATCTATTCCC TGGATAAAAT TTAAAAGTAA TTGCTACAGT TTTTCTACAG TCCTAGACAG TATGAGTTTT GAGGCTGCTC ATGAATTTTG CAAAAAGGAA GGTTCTAATC TTTTAACAAT CAAGGATGAG GCTGAAAATG CATTTCTCCT AGAAGAGCTG TTTGCTTTTG GTTCTTCTGT CCAGATGGTT TGGTTGAATG CTCAATTTGA TGGTAACAAT GAAACCATAA AGTGGTTTGA TGGAACTCCC ACAGACCAGT CAAACTGGGG CATTCGGAAG CCAGACACAG ACTACTTCAA GCCCCATCAT TGTGTTGCCT TGAGGATCCC TGAAGGATTA TGGCAGCTAT CCCCGTGTCA AGAAAAAAAA GGCTTTATAT GTAAAATGGA GGCAGATATT CACACTGCAG AGGCGCTGCC AGAAAAAGGA CCAAGTCACA GCATCATTCC TCTTGCGGTT GTACTGACAC TGATAGTCAT TGTGGCCATT TGCACACTTT CCTTCTGCAT ATACAAGCAT AACGGTGGCT TCTTCAGGAG ACTTGCAGGG TTTCGGAATC CTTACTATCC TGCAACCAAC TTTAGTACAG TATATTTAGA AGAAAATATT CTCATTTCTG ATCTTGAGAA GAGTGACCAA CTCGAGGCTA CTAACTTCAG CCTGCTGAAG CAGGCTGGAG ACGTGGAGGA GAACCCTGGA CCTATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA GTAACGCGTC GACCAGTGGT CGACCCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT ACCATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG CCAGTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCCT GGAATTCGAG CTCGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA AGACAAGATC TGCTTTTTGC TTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT AGTAGTTCAT GTCATCTTAT TATTCAGTAT TTATAACTTG CAAAGAAATG AATATCAGAG AGTGAGAGGA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GCTCTAGCTA TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG GACGTACCCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAG
