pPSdown2
$99.00
In stock
SKU
PL42440
100μg liquid plasmid(Instantly):$120
| name | pPSdown2 | another name | apPSdown2 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Signal report | Fragment type | Promoter |
| Fragment species | Empty vector | Prokaryotic resistance | Spe |
| Selective marker | Bar | Fluorescent labeling | Green |
| Promoter | Replicon | pSa | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTAAAACGAC GGCCAGTGAG CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGGTACCG GGCCCCCCCT CGAGGTCGAC GGTATCGATA AGCTTGATAT CGAATTCCGC GGCCGCAGGA GCTGCCGTCT TCAGTGCCTG CAGGCATAGA AGACCCGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCTTCAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCACG GCATGGACGA GCTGTACAAG TAAAGCGGCC GCAAATTGCC CAGAATTAAT TCCCCAACTG CACAGAACAA ATTTCCCTTT GCTTTTGTGT AGACCTCAAA ACTTTATCCC CCATCTTTGA TCTTATCCCT TGTTTTTCTG CTTTTTTCTT CTTTCTTGGG TTTTGATTTC CGGACTTAAC GTTTGTTTTC CGGTTTGCGA GACATATTCT ATCGGATTCT CAACTGTCTG ATGAAATAAA TATGTAATGT TCTATAAGTC TTTCAATTTG ATGGGATGTT CCACTTCTGT ATAGGAGCCA ATTGATTGAC AACATGCATC AATCGGAGCT CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTGCGCGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCTCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCACTCC GAGACCACAA GGCGCGCCTA GTGGTCTCCA GGAAGGAAAG AACATGAAGG CCTTGACAGG ATATATTGGC GGGTAAACTA AGTCGCTGTA TGTGTTTGTT TGAGATCTCA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAGAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGTG TAACATTGGT CTAGTGATTA TTTGCCGACT ACCTTGGTGA TCTCGCCTTT CACGTAGTGA ACAAATTCTT CCAACTGATC TGCGCGCGAG GCCAAGCGAT CTTCTTGTCC AAGATAAGCC TGCCTAGCTT CAAGTATGAC GGGCTGATAC TGGGCCGGCA GGCGCTCCAT TGCCCAGTCG GCAGCGACAT CCTTCGGCGC GATTTTGCCG GTTACTGCGC TGTACCAAAT GCGGGACAAC GTAAGCACTA CATTTCGCTC ATCGCCAGCC CAGTCGGGCG GCGAGTTCCA TAGCGTTAAG GTTCCATTTA GCGCCTCAAA TAGATCCTGT TCAGGAACCG GATCAAAGAG TTCCTCCGCC GCTGGACCTA CCAAGGCAAC GCTATGTTCT CTTGCTTTTG TCAGCAAGAT AGCCAGATCA ATGTCGATCG TGGCTGGCTC GAAGATACCT GCAAGAATGT CATTGCGCTG CCATTCTCCA AATTGCAGTT CGCGCTTAGC TGGATAACGC CACGGAATGA TGTCGTCGTG CACAACAATG GTGACTTCTA CAGCGCGGAG AATCTCGCTC TCTCCAGGGG AAGCCGAAGT TTCCAAAAGG TCGTTGATCA AAGCTCGCCG CGTTGTTTCA TCAAGCCTTA CGGTCACCGT AACCAGCAAA TCAATATCAC TGTGTGGCTT CAGGCCGCCA TCCACTGCGG AGCCGTACAA ATGTACGGCC AGCAACGTCG GTTCGAGATG GCGCTCGATG ACGCCAACTA CCTCTGATAG TTGAGTCGAT ACTTCGGCGA TCACCGCTTC CCTCATAACA CCCCTTGTAT TACTGTTTAT GTAAGCAGAC AGTTTTATTG TTCATGATGA TATATTTTTA TCTTGTGCAA TGTAACATCA GAGATTTTGA GACACAACGT GGCTTTGTTG AATAAATCGA ACTTTTGCTG AGTTGAAGGA TCAGATCACG CATCTTCCCG ACAACGCAGA CCGTTCCGTG GCAAAGCAAA AGTTCAAAAT CACCAACTGG TCCACCTACA ACAAAGCTCT CATCAACCGT GGCTCCCTCA CTTTCTGGCT GGATGATGGG GCGATTCAGG CGATCCCCAT CCAACAGCCC GCCGTCGAGC GGGCTTTTTT ATCCCCGGAA GCCTGTGGAT AGAGGGTAGT TATCCACGTG AAACCGCTAA TGCCCCGCAA AGCCTTGATT CACGGGGCTT TCCGGCCCGC TCCAAAAACT ATCCACGTGA AATCGCTAAT CAGGGTACGT GAAATCGCTA ATCGGAGTAC GTGAAATCGC TAATAAGGTC ACGTGAAATC GCTAATCAAA AAGGCACGTG AGAACGCTAA TAGCCCTTTC AGATCAACAG CTTGCAAACA CCCCTCGCTC CGGCAAGTAG TTACAGCAAG TAGTATGTTC AATTAGCTTT TCAATTATGA ATATATATAT CAATTATTGG TCGCCCTTGG CTTGTGGACA ATGCGCTACG CGCACCGGCT CCGCCCGTGG ACAACCGCAA GCGGTTGCCC ACCGTCGAGC GCCTTTGCCC ACAACCCGGC GGCCGGCCGC AACAGATCGT TTTATAAATT TTTTTTTTTT GAAAAAGAAA AAGCCCGAAA GGCGGCAACC TCTCGGGCTT CTGGATTTCC GATCCCCGGA ATTAGATCTT GGCAGGATAT ATTGTGGTGT AACGTTATCA GCTTGCATGC CGGTCGATCT AGTAACATAG ATGACACCGC GCGCGATAAT TTATCCTAGT TTGCGCGCTA TATTTTGTTT TCTATCGCGT ATTAAATGTA TAATTGCGGG ACTCTAATCA TAAAAACCCA TCTCATAAAT AACGTCATGC ATTACATGTT AATTATTACA TGCTTAACGT AATTCAACAG AAATTATATG ATAATCATCG CAAGACCGGC AACAGGATTC AATCTTAAGA AACTTTATTG CCAAATGTTT GAACGATCTG CTTGACTCTA GGGGTCATCA GATTTCGGTG ACGGGCAGGA CCGGACGGGG CGGCACCGGC AGGCTGAAGT CCAGCTGCCA GAAACCCACG TCATGCCAGT TCCCGTGCTT GAAGCCGGCC GCCCGCAGCA TGCCGCGGGG GGCATATCCG AGCGCCTCGT GCATGCGCAC GCTCGGGTCG TTGGGCAGCC CGATGACAGC GACCACGCTC TTGAAGCCCT GTGCCTCCAG GGACTTCAGC AGGTGGGTGT AGAGCGTGGA GCCCAGTCCC GTCCGCTGGT GGCGGGGGGA GACGTACACG GTGGACTCGG CCGTCCAGTC GTAGGCGTTG CGTGCCTTCC AGGGACCCGC GTAGGCGATG CCGGCGACCT CGCCGTCCAC CTCGGCGACG AGCCAGGGAT AGCGCTCCCG CAGACGGACG AGGTCGTCCG TCCACTCCTG CGGTTCCTGC GGCTCGGTAC GGAAGTTGAC CGTGCTTGTC TCGATGTAGT GGTTGACGAT GGTGCAGACC GCCGGCATGT CCGCCTCGGT GGCACGGCGG ATGTCGGCCG GGCGTCGTTC TGGGCTCATG GTAGATCCCC TCGATCGAGT TGAGAGTGAA TATGAGACTC TAATTGGATA CCGAGGGGAA TTTATGGAAC GTCAGTGGAG CATTTTTGAC AAGAAATATT TGCTAGCTTA TAGTGACCTT AGGCGACTTT TGAACGCGCA ATAATGGTTT CTGACGTATG TGCTTAGCTC ATTAAACTCC AGAAACCCGC GGCTCAGTGG CTCCTTCAAC GTTGCGGTTC TGTCAGTTCC AAACGTAAAA CGGCTTGTCC CGCGTCATCG GCGGGGGTCA TAACGTGACT CCCTTAATTC TCCGCTCATG ATCGATAACA TTAACGCTTA CAATTTCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTT
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