pSV40-hRluc-5UTR-SRD5A2(human)-3UTR-Fluc
$84.00
In stock
SKU
PL42408
100μg liquid plasmid(Instantly):$105
| name | pSV40-hRluc-5UTR-SRD5A2(human)-3UTR-Fluc | another name | apSV40-hRluc-5UTR-SRD5A2(human)-3UTR-Fluc |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | UTR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Rluc,Fluc | |
| Promoter | SV40 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | psiCHECK-2-F：GAAGTTCCCTAACACCGAGT | |
| 3′ sequencing primer | Psicheck-2-R：CCCCCGCGAGGTCCGAAGAC | Copy number | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT CTGCATGGTA ACGCTGCCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC TCCTACGAGC ACCAAGACAA GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG ATCGAGTCCT GGGACGAGTG GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA GAGGGCGAGA AAATGGTGCT TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG ATCATGCGGA AACTGGAGCC TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG GGCGAGGTTA GACGGCCTAC CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC AAGCCCGACG TCGTCCAGAT TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT CTGCCTAAGA TGTTCATCGA GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA GCTAAGAAGT TCCCTAACAC CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG GACGCTCCAG ATGAAATGGG TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC GAGCAGTAAT TCTAGGCGAT CGCTCGAGCT GGGAGGGCAG CGGCCACCGG CGAGGAACAC GGCGCGATGC AGGTTCAGTG CCAGCAGAGC CCAGTGCTGG CAGGCAGCGC CACTTTGGTC GCCCTTGGGG CACTGGCCTT GTACGTCGCG AAGCCCTCCG GCTACGGGAA GCACACGGAG AGCCTGAAGC CGGCGGCTAC CCGCCTGCCA GCCCGCGCCG CCTGGTTCCT GCAGGAGCTG CCTTCCTTCG CGGTGCCCGC GGGGATCCTC GCCCGGCAGC CCCTCTCCCT CTTCGGGCCA CCTGGGACGG TACTTCTGGG CCTCTTCTGC CTACATTACT TCCACAGGAC ATTTGTGTAC TCACTGCTCA ATCGAGGGAG GCCTTATCCA GCTATACTCA TTCTCAGAGG CACTGCCTTC TGCACTGGAA ATGGAGTCCT TCAAGGCTAC TATCTGATTT ACTGTGCTGA ATACCCTGAT GGGTGGTACA CAGACATACG GTTTAGCTTG GGTGTCTTCT TATTTATTTT GGGAATGGGA ATAAACATTC ATAGTGACTA TATATTGCGC CAGCTCAGGA AGCCTGGAGA AATCAGCTAC AGGATTCCAC AAGGTGGCTT GTTTACGTAT GTTTCTGGAG CCAATTTCCT CGGTGAGATC ATTGAATGGA TCGGCTATGC CCTGGCCACT TGGTCCCTCC CAGCACTTGC ATTTGCATTT TTCTCACTTT GTTTCCTTGG GCTGCGAGCT TTTCACCACC ATAGGTTCTA CCTCAAGATG TTTGAGGACT ACCCCAAATC TCGGAAAGCC CTTATTCCAT TCATCTTTTA AAGGAACCAA ATTAAAAAGG AGCAGAGCTC CCACAATGCT GATGAAAACT GTCAAGCTGC TGAAACTGTA ATTTTCATGA TATAATAGTC CCGTATATAT GTAATAGTAG GTCTCCTGGC GTTCTGCCAG CTGGCCTGGG GATTCTGAGT GGTGTCTGCT TAGAGTTTAC TCCTACCCTT CCAGGGACCC CTATCCTGAT CCCCAACTGA AGCTTCAAAA AGCCACTTTT CCAAATGGCG ACAGTTGCTT CTTAGCTATT GCTCTGAGAA AGTACAAACT TCTCCTATGT CTTTCACCGG GCAATCCAAG TACATGTGGC TTCATACCCA CTCCCTGTCA ATGCAGGACA ACTCTGTAAT CAAGAATTTT TTGACTTGAA GGCAGTACTT ATAGACCTTA TTAAAGGTAT GCATTTTATA CATGTAACAG AGTAGCAGAA ATTTAAACTC TGAAGCCACA AAGACCCAGA GCAAACCCAC TCCCAAATGA AAACCCCAGT CATGGCTTCC TTTTTCTTGG TTAATTAGGA AAGATGAGAA ATTATTAGGT AGACCTTGAA TACAGGAGCC CTCTCCTCAT AGTGCTGAAA AGATACTGAT GCATTGACCT CATTTCAAAT TTGTGCAGTG TCTTAGTTGA TGAGTGCCTC TGTTTTCCAG AAGATTTCAC AATCCCCGGA AAACTGGTAT GGCTATTCTT GAAGGCCAGG TTTTAATAAC CACAAACAAA AAGGCATGAA CCTGGGTGGC TTATGAGAGA GTAGAGAACA ACATGACCCT GGATGGCTAC TAAGAGGATA GAGAACAGTT TTACAATAGA CATTGCAAAC TCTCATGTTT TTGGAAACTA GTGGCAATAT CCAAATAATG AGTAGTGTAA AACAAAGAGA ATTAATGATG AGGTTACATG CTGCTTGCCT CCACCAGATG TCCACAACAA TATGAAGTAC AGCAGAAGCC CCAAGCAACT TTCCTTTCCT GGAGCTTCTT CCTTGTAGTT CTCAGGACCT GTTCAAGAAG GTGTCTCCTA GGGGCAGCCT GAATGCCTCC CTCAAAGGAC CTGCAGGCAG AGACTGAAAA TTGCAGACAG AGGGGCACGT CTGGGCAGAA AACCTGTTTT GTTTGGCTCA GACATATAGT TTTTTTTTTT TTTACAAAGT TTCAAAAACT TAAAAATCAG GAGATTCCTT CATAAAACTC TAGCATTCTA GTTTCATTTA AAAAGTTGGA GGATCTGAAC ATACAGAGCC CACATTTCCA CACCAGAACT GGAACTACGT AGCTAGTAAG CATTTGAGTT TGCAAACTCT TGTGAAGGGG TCACCCCAGC ATGAGTGCTG AGATATGGAC TCTCTAAGGA AGGGGCCGAA CGCTTGTAAT TGGAATACAT GGAAATATTT GTCTTCTCAG GCCTATGTTT GCGGAATGCA TTGTCAATAT TTAGCAAACT GTTTTGACAA ATGAGCACCA GTGGTACTAA GCACAGAAAC TCACTATATA AGTCACATAG GAAACTTGAA AGGTCTGAGG ATGATGTAGA TTACTGAAAA ATGCAAATTG CAATCATATA AATAAGTGTT TTTGTTGTTC ATTAAATACC TTTAAATCAT GGATGTTAAG CAGTTTTGTT TGAATTAAAA TATCCTGGGA CTTCTGGAGA TTTTCACAAG GTATTGGGGT GTGCTTCCCT GTCCCGCCTG TCCCTCATTC CCATTCACCC TGTCTGTAGT GTCGTTTCCT TTCTTCTCTT CTCTCTTCAT GAAATTAGGG GCTGTGTGCT GGAAATAAAG AAGTATGACT TATACAGCCT CTCAGCCACA ATTCAGAACT ACAAGGAAGT ACAATAAAGG TGCAGGAAAA CGTCATGAGG TTAGATGCCA CTCCTGGTGC TGAGGAATAC ACAGGGGGAA TGAACACACT TGGTGAAGTA AATCTTCAGG TGTAGCTTGA GCTCTGTGAC ATAAAAGACT TCCAGTTCTC TCCATGACAT TGAAAACTAC CTTGCTTAAG TCCTTTATTT TACTACCATG TAAAACATCC AGGCCGGTCC TTGAGATGAA ATAAATATAC AATAAATGGC AGTACTCAGT CTCAGGACAA AACCGCCATT TGGCAATTTC ACTTTTTGCC ACAAGAGGGC GCTTTCTAAA ATAGTATTTA GTCCAACCTT GACTTAATTA GGTCCAACCC CAGTCCTGTT GCCTGATCGT GGACCTTTCA AGTTAGCATG ATCGTGGAAC TTCTTAATCT GAATATAGGG TAGAGTAGCG TGAACTCTAT GAAAGCTGAA ACCTTCCCTT GATAATTCAG GTATTCTCAG TGTTCTAGGG CTGTTGTGTT CAATGACAGT TTGTCATTGT TTAGGGCTAG GATATGTATG TGGATTAAAT ACAGTAAAGG TCTGAACATG TGCTCATCTC TAAACATATT TCTTCCCACT TTTAAAAATA GGGTGCAACA AAATTATAGT TAATTGAGGT TTCCATGGAC TGTTTTTTGG CTAAAATAAT CTAACAGTGT CCTAGCTCAC AAGATACTCT ATCTTGGTCT TGCTGAAGCT TTCACGGAAG AGGCGTTTTC CTGCCAACCT TGAAGAAAAT GTTGAGTTTT GTTTGTTTTT CAGAATTTAG ACTTAAAGAG CTGAAGCAGA CCTCAGACAT CAGCTATTCC TACCACTTCA CATTATAGGT AGTGACACTG AGACCCACAG AGTTAAAAGG CCTCCTCACA GCTGGTACAG AGCTGGTCAG AGCAGCCATC TCATTTTGTC CCTTTGAGGG TCAGATGTAT GCTTCTTCTG CTTAAGGCAC AGCCTTTAGG GCTGCCATCT GGATTTCTAC AGAGCTCATG CAGGAGAAAA ATAGCCAGAG CTTAAGTGCT GTTGCATAGT TCAGTTTATA CACCTATTGT CCCATGCCTG TTTGTGTGTG AGGCTTCCCT CGCTCCGTGG GAGCACCAGG CTCATGCCCT TTCATAAACC TATTTGACAG AATAGAGAGT TTTCCCTCAT TGGTGTAGTC AAGACAACAT GTGTGTTCAG CATGGAAAAA AGGTTTCAGG TCATAAACCC CCTTATCCCT AATGCTTACT TTCATAAAGA ACAACAGTGG CTCCACTGTA TTCTAAAAGG AAAAAGGAGC TCTAATGAGA AATACGCAAT TAAACAGCTA TTTAGGCGGC TTAGACCCTT TCAGGAACAA ATAAATAAAT CCGTGGTTTT ACAACCCTGA AGTTTGATAA TAGCTCTCTT GGATTTTTGT GGTTACCTCA TGGCAATCTC ACTAAGAAGC AGGGGAGCAA GCTCTTGGCA CATGAAGGAA TAGGCTCATG AAGATTCATT TCTTTTCCCG CTTGGCCTTT TGGTTCTCTA ATAAGCTGTG AGCTGAGGTC CATGCAGGGA AAACAAGGAG GGTAACGGCA AATGTAAAGT GAATTTTTCT GGAGAAGGAT GCTGATTATT TTATAGCATC TACACTATCC TATGATGAAA GAGGCATAGA GCCGGGTGGC AATGTGAGTT CTTGGGTGAT TTAATAAGCC ATCGAGTGGA TTTAATTTCT AAGTAAGACA TTTGTTTATT CTTTGTACAA GTCCATGGAG TTGTGATCTT AAAGAGTTCT GTGTTATTTG CATTGTGCCT CATTTCTCCC CATCAACCTT AGCTTGAGAT TTGGATGAAT AAAATAAATA ACTGGAGAAG GTTAAAGTAT GCACCAACCC GGGAATTCGT TTAAACCTAG AGCGGCCGCT GGCCGCAATA AAATATCTTT ATTTTCATTA CATCTGTGTG TTGGTTTTTT GTGTGAGGAT CTAAATGAGT CTTCGGACCT CGCGGGGGCC GCTTAAGCGG TGGTTAGGGT TTGTCTGACG CGGGGGGAGG GGGAAGGAAC GAAACACTCT CATTCGGAGG CGGCTCGGGG TTTGGTCTTG GTGGCCACGG GCACGCAGAA GAGCGCCGCG ATCCTCTTAA GCACCCCCCC GCCCTCCGTG GAGGCGGGGG TTTGGTCGGC GGGTGGTAAC TGGCGGGCCG CTGACTCGGG CGGGTCGCGC GCCCCAGAGT GTGACCTTTT CGGTCTGCTC GCAGACCCCC GGGCGGCGCC GCCGCGGCGG CGACGGGCTC GCTGGGTCCT AGGCTCCATG GGGACCGTAT ACGTGGACAG GCTCTGGAGC ATCCGCACGA CTGCGGTGAT ATTACCGGAG ACCTTCTGCG GGACGAGCCG GGTCACGCGG CTGACGCGGA GCGTCCGTTG GGCGACAAAC ACCAGGACGG GGCACAGGTA CACTATCTTG TCACCCGGAG GCGCGAGGGA CTGCAGGAGC TTCAGGGAGT GGCGCAGCTG CTTCATCCCC GTGGCCCGTT GCTCGCGTTT GCTGGCGGTG TCCCCGGAAG AAATATATTT GCATGTCTTT AGTTCTATGA TGACACAAAC CCCGCCCAGC GTCTTGTCAT TGGCGAATTC GAACACGCAG ATGCAGTCGG GGCGGCGCGG TCCCAGGTCC ACTTCGCATA TTAAGGTGAC GCGTGTGGCC TCGAACACCG AGCGACCCTG CAGCGACCCG CTTAAAAGCT TGGCATTCCG GTACTGTTGG TAAAGCCACC ATGGCCGATG CTAAGAACAT TAAGAAGGGC CCTGCTCCCT TCTACCCTCT GGAGGATGGC ACCGCTGGCG AGCAGCTGCA CAAGGCCATG AAGAGGTATG CCCTGGTGCC TGGCACCATT GCCTTCACCG ATGCCCACAT TGAGGTGGAC ATCACCTATG CCGAGTACTT CGAGATGTCT GTGCGCCTGG CCGAGGCCAT GAAGAGGTAC GGCCTGAACA CCAACCACCG CATCGTGGTG TGCTCTGAGA ACTCTCTGCA GTTCTTCATG CCAGTGCTGG GCGCCCTGTT CATCGGAGTG GCCGTGGCCC CTGCTAACGA CATTTACAAC GAGCGCGAGC TGCTGAACAG CATGGGCATT TCTCAGCCTA CCGTGGTGTT CGTGTCTAAG AAGGGCCTGC AGAAGATCCT GAACGTGCAG AAGAAGCTGC CTATCATCCA GAAGATCATC ATCATGGACT CTAAGACCGA CTACCAGGGC TTCCAGAGCA TGTACACATT CGTGACATCT CATCTGCCTC CTGGCTTCAA CGAGTACGAC TTCGTGCCAG AGTCTTTCGA CAGGGACAAA ACCATTGCCC TGATCATGAA CAGCTCTGGG TCTACCGGCC TGCCTAAGGG CGTGGCCCTG CCTCATCGCA CCGCCTGTGT GCGCTTCTCT CACGCCCGCG ACCCTATTTT CGGCAACCAG ATCATCCCCG ACACCGCTAT TCTGAGCGTG GTGCCATTCC ACCACGGCTT CGGCATGTTC ACCACCCTGG GCTACCTGAT TTGCGGCTTT CGGGTGGTGC TGATGTACCG CTTCGAGGAG GAGCTGTTCC TGCGCAGCCT GCAAGACTAC AAAATTCAGT CTGCCCTGCT GGTGCCAACC CTGTTCAGCT TCTTCGCTAA GAGCACCCTG ATCGACAAGT ACGACCTGTC TAACCTGCAC GAGATTGCCT CTGGCGGCGC CCCACTGTCT AAGGAGGTGG GCGAAGCCGT GGCCAAGCGC TTTCATCTGC CAGGCATCCG CCAGGGCTAC GGCCTGACCG AGACAACCAG CGCCATTCTG ATTACCCCAG AGGGCGACGA CAAGCCTGGC GCCGTGGGCA AGGTGGTGCC ATTCTTCGAG GCCAAGGTGG TGGACCTGGA CACCGGCAAG ACCCTGGGAG TGAACCAGCG CGGCGAGCTG TGTGTGCGCG GCCCTATGAT TATGTCCGGC TACGTGAATA ACCCTGAGGC CACAAACGCC CTGATCGACA AGGACGGCTG GCTGCACTCT GGCGACATTG CCTACTGGGA CGAGGACGAG CACTTCTTCA TCGTGGACCG CCTGAAGTCT CTGATCAAGT ACAAGGGCTA CCAGGTGGCC CCAGCCGAGC TGGAGTCTAT CCTGCTGCAG CACCCTAACA TTTTCGACGC CGGAGTGGCC GGCCTGCCCG ACGACGATGC CGGCGAGCTG CCTGCCGCCG TCGTCGTGCT GGAACACGGC AAGACCATGA CCGAGAAGGA GATCGTGGAC TATGTGGCCA GCCAGGTGAC AACCGCCAAG AAGCTGCGCG GCGGAGTGGT GTTCGTGGAC GAGGTGCCCA AGGGCCTGAC CGGCAAGCTG GACGCCCGCA AGATCCGCGA GATCCTGATC AAGGCTAAGA AAGGCGGCAA GATCGCCGTG TAATAATTCT AGAGTCGGGG CGGCCGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAA AATCGATAAG GATCCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGGCTCG AC
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