NM_029078.3;2500001H09Rik; 5730417B17Rik
100μg liquid plasmid(Instantly):$134
| name | pLV3-CMV-Pcf11(mouse)-3×FLAG-CopGFP-Puro | another name | apLV3-CMV-Pcf11(mouse)-3×FLAG-CopGFP-Puro |
| Insert Size (bp) | 4662bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGT CAGAGCAGAC GCCGGCGGAG GCCGGGGCTG CGGGGGCCCG GGAGGACGCC TGTCGGGATT ATCAGTCGTC GCTGGAAGAC CTGACCTTCA ATAGCAAACC GCACATCAAT ATGCTGACCA TTCTAGCCGA GGAGAACCTG CCCTTCGCCA AGGAGATCGT CTCTCTCATC GAGGCCCAAA CCGCCAAGGC TCCTTCCTCA GAGAAGCTTC CAGTTATGTA CCTTATGGAT TCTATCGTGA AAAATGTTGG AAGAGAATAT CTCACTGCCT TTACTAAAAA TCTAGTTGCA ACATTTATTT GTGTGTTTGA AAAGGTGGAT GAAAATACTA GAAAAAGTTT ATTTAAATTA CGTTCCACAT GGGATGAAAT ATTCCCTTTG AAGAAACTTT ATGCCTTGGA TGTCAGAGTC AATTCATTAG ATCCTGCTTG GCCTATTAAA CCTCTGCCTC CTAATGTGAA TACATCTAGC ATCCATGTGA ATCCCAAATT TTTAAATAAA TCGCCTGATG AGCCTTCAAC ACCTGGCACA GTGGTCAGTT CCCCCAGTAT CTCCACTCCT CCCATTGTAC CTGATATACA AAAGAATCTT ACCCAAGAAC AACTAATAAG GCAACAGTTA CTGGCAAAAC AAAAACAGTT GTTAGAACTT CAGCAGAAAA AGCTGGAGCT TGAGTTAGAA CAAGCTAAGG CACAGCTGGC AGTATCTCTG AGTGTTCAGC AGGAAACAGC CAACTTGGGT CCTGGATCAG TGCCGTCTAA ATTACATGTT CCACAAATTC CCACAATGGC TGTTAAAACT CCCCATCAGG TCCCTGTACA ACCTGACAAA AGCCGAGCAG GTCCATCCTT ACAAATGCAA GACTTGAAAG GAACTAACCG GGATCCCCGT CTTAACAGGA TGAGCCAACA TTCTTCCCAT GGCAAAGAGC AGAGTCATAG GAAAGAATTT GTAATGAACA CAATAAACCA GTCTGATATT AAAACAAGTA AGAATGTACC CTCTGAAAAG CTAAATTCAT CCAAACAAGA AAAAAGTAAA TCAGGTGAAA GAATAACCAA GAAAGAACTT GACCAGTTAG ATTCTAAATC CAAATCTAAG TCTAAATCAC CATCACCTTT GAAAAACAAA TTATCCCACA CAAAAGACTT GAAAAATCAA GACTCTGAAA GTATGAGGTT GTCTGATATG AGTAAGAGAG ATCCACGATT AAAGAAGCAT CTTCAGGATA AAGCTGAGGG CAAAGATGAA GATGTAAAAG AAAAGAGAAA AACTGCAGAA AAGAAGGAGA AAGATGAGCA CATGAAATCA TCTGAACACA GGGTGATTGG AAGTAGAAGT AAAATCATAA ATGGCATTGT CCAAAAACAA GACATGGTTA CAGAGGAGTT GGAAAAACAG GGGACAAAAC CAGGGAGATC GAGTACTAGA AAGAGATCAA GATCAAGGTC ACCTAAGTCT CGGTCACCAA TTATACATTC CCCAAAGAGA AGAGATAGAC GGTCACCCAA ACGAAGGCAA AGAAGTATGT CTCCCAATTT GGCACCCAAA GCTGGAAAGA TGCGTCAGTC GGGACTAAAA CAGTCACATA TGGAAGAATT CCCACCACCT TCCAGGGAAG AAAGAAATAT TAAGAGAAGT GCTAAGCAGG ATGTTAGAGA TCCCAGACGA CTGAAAAAGA TGGATGAGGA CCGGCCTCAA GAAACTGCAG GTCAACATTC TATGAAGTCA GGTGGTGACC CAAAGGAGAA TATAGAGAAT TGGCAAAGCT CTAAGTCTGC CAAAAGATGG AAATCTGGCT GGGAAGAAAA TAAAAGCTTG CAGCAGGGTG ATGAACATAG TAAACCTCCT CATCTAAGGC ATAGGGAGAG CTGGTCAAGC ACTAAAGGGA TCTTGTCACC TCGAGCCCCC AAGCAGCAGC ACCGACTAAG TGTAGATGCC AATCTTCAAA TTCCTAAAGA GTTAACTCTT GCAAGCAAAA GAGAATTGCT TCAAAAGACG AGCGAACGTT TAGCATCTGG TGAAATTACA CAGGATGAGT TCCTTGTTGT TGTGCATCAA ATTCGACAGC TATTTCAGTA TCAAGAAGGT GTACGAGAGG AGCAGAGATC ACCATTCAAT GATCGTTTTC CACTTAAGCG ACCTAGATAT GAAGATTCAG ATAAACCATT TGTAGATGGC CCAGCATCAA GATTTGCTGG CCTTGATACA AATCAGCGAC TTACAGCTCT AGCTGAAGAC CGACCATTAT TTGATGGACC TGGTAGGCCA TCTGTGACGA GAGATGGCCC AGCCAAGATG ATTTTTGAAG GACCTAATAA ATTAAGCCCT AGAATTGATG GACCTCCTAC ACCAGGTTCT CTTCGGTTTG ATGGGTCACC AGGACAAATG GGGGGAGGAG GCCCTATGAG ATTTGAAGGA CCACAAGGTC AGTTAGGAGG TGGGTGTCCT TTGAGATTTG AAGGTCCTCC AGGACCAGTA GGAACACCTC TGCGGTTTGA AGGGCCAATT GGTCAAGGAG GAGGAGGTGG TTTTCGATTT GAAGGTTCAC CTAGTTTGAG GTTTGAGGGA TCTACAGGTG GTTTACGATT TGAAGGACCA GGGGGTCAGC CCGTGGGTGG TCTCAGGTTT GAAGGACATC GTGGTCAACC TGTGGGTGGT CTCAGGTTTG AAGGACCTCA TGGACAGCCT GTGGGCAGTC TTAGATTTGA TAATCCTCGA GGTCAGCCTG TAGGAGGACT TAGATTTGAA GGGGGTCATG GTCCATCAGG GGCTGCAATT AGGTTTGATG GGCCTCATGG TCAGCCAGGT GGTGGTGGTG GTATCAGATT TGAGGGCCCT TTGCTACAGC AAGGAGTTGG AATGAGGTTT GAGGGTCCCC ATGGTCAGTC TGTAGCTGGT CTGAGATTTG AAGGACATAA TCAACTAGGT GGGAACCTTA GGTTTGAGGG ACCACATGGT CAACCAGGGG TTGGGATCAG GTTTGAAGGA CCTATAGTTC AACAAGGAGG TGGAATGAGG TTTGAAGGTC CTGTACCAGG AGGTGGCCTG AGAATTGAAG GGCCTCTGGG TCAAGGTGGT CCTAGATTTG AAGGTTGTCA CTCTTTAAGG TTTGATGGAC AGCCAGGTCA ACCATCACTT TTGCCAAGAT TTGATGGATT ACATGGCCAG CCAGGTCCTA GATTTGAAAG AACTGGTCAG CCAGGTCCAC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCAAG ATTTGATGGT GTACCTCAAA GATTTGATGG GCCACAACAC CAGCAAGCAT CAAGATTTGA TATTCCTCTT GGTCTACAAG GAACTCGATT TGACAATCAT CCTTCACAAA GGATTGAATC TTTCAATCAT TCTGGCCCAT ATAATGATCC ACCTGGCAAT ACTTTTAATG TTCCATCTCA AGGATTACAG TTCCAAAGAC ACGAACAAAT ATTTGATACA CCTCAAGGAC CAAATTTTAA TGGACCACAT GGCCCTGGAA ATCAGAATTT CCCAAATCCC ATTAACAGAG CTTCTGGACA CTATTTTGAT GAAAAGAATC TTCAGAGTTC TCAGTTTGGA AACTTTGGCA ATTTGCCTAC ACCAATATCA GTAGGAAATA TTCAGGCATC TCAACAGGTT CTTACTGGTG TTGCTCAGCC AGTAGCGTTT GGCCAAGGAC AACAGTTCTT ACCAGTTCAT CCACAGAATC CTGGAGCTTT TATTCAAAAT CCTTCAGGCG GTCTTCCCAA GGCATATCCT GATAATCATC TCAGTCAGGT GGATGTAAAC GAATTGTTTT CAAAACTGCT AAAAACAGGA ATTCTCAAAT TGTCACAGCC TGATTCAGCT ACAGCACAGG TAACCGAGGC TGTTGCTCAG CCTCCCCCTG AGGAGGACGA GGATCAGAAT GAAGATCAGG ATGTTCCAGA TCTTACCAAT TTCACCATTG AAGAATTGAA GCAACGTTAT GACAGTGTTA TAAACCGGCT GTACACTGGG ATTCAATGTT ACTCGTGTGG AATGAGGTTC ACGACATCCC AGACAGATGT TTATGCAGAT CACTTGGATT GGCATTATCG GCAAAACAGA ACCGAGAAAG ATGTTAGCAG AAAAGTCACT CATAGACGTT GGTACTACAG TCTAACAGAT TGGATAGAAT TTGAAGAAAT AGCTGATCTG GAAGAACGTG CAAAAAGCCA GTTTTTTGAA AAGGTTCATG AAGAGGTTGT CCTTAAAACT CAGGAGGCTG CTAAAGAAAA GGAGTTCCAA AGTGTACCTG CTGGACCAGC TGGAGCAGTT GAGAGTTGTG AAATCTGTCA AGAACAATTT GAACAGTACT GGGATGAAGA GGAGGAAGAA TGGCACTTAA AAAATGCTAT TAGAGTAGAC GGAAAGATTT ACCATCCATC ATGTTATGAA GATTATCAAA ATACATCTTC ATTTGATTGC ACACCATCTC CCAGCAAGAC ACCAGTTGAA AACCCTTTGA ACATTATGTT GAACATTGTC AAAAACGAAT TGCAAGAACC CTGTGAAAGT CCCAAAGTTA AGGAAGAACA AATTGATGCC CCACCAGCTT GTTCAGAAGA AAGTGTAGCA ACACCCACTG AAATTAAAAC AGAAAGTGAT ACAGTTGAGT CAGTTGGATC CGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGTAG CGGCCGCGAA GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC CCGCCGCCCT ACCTGAGGCC GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC CTGTGGTGCC TCCTGAACTG CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC GGGCCTTTGT CCGGCGCTCC CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG CCTGACCCTG CTTGCTCAAC TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG ATCCAAGCTG TGACCGGCGC CTACGCTAGA CGCCACCATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCAAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGGTGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGTGCTGGT GGGCAGCTTC GCCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT TCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGCTGCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC CCATCGCCTT CGCCAGATCC CGCGCTCAGT CGTCCAATTC TGCCGTGGAC GGCACCGCCG GACCCGGCTC CACCGGATCT CGCGAGGGCA GAGGAAGTCT TCTAACATGC GGTGACGTGG AGGAGAATCC CGGCCCTATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA TCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGAAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAGCTGACGT CCTTTCCATG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTCCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGCCGCCTC CCCGCCTGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAAATAA GATCTGCTTT TTGCTTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTAGTAGT TCATGTCATC TTATTATTCA GTATTTATAA CTTGCAAAGA AATGAATATC AGAGAGTGAG AGGAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGCTCTA GCTATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGACTTTTGC AGAGACGGCC CAAATTCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGCCAAGC TG
