pTRE2-SORCS3(human)-Puro
$113.00
In stock
SKU
PL42303
NM_014978.3;SORCS
100μg liquid plasmid(Instantly):$134
| name | pTRE2-SORCS3(human)-Puro | another name | apTRE2-SORCS3(human)-Puro |
| Insert Size (bp) | 3669bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | TRE | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTCGAGTTTA CCACTCCCTA TCAGTGATAG AGAAAAGTGA AAGTCGAGTT TACCACTCCC TATCAGTGAT AGAGAAAAGT GAAAGTCGAG TTTACCACTC CCTATCAGTG ATAGAGAAAA GTGAAAGTCG AGTTTACCAC TCCCTATCAG TGATAGAGAA AAGTGAAAGT CGAGTTTACC ACTCCCTATC AGTGATAGAG AAAAGTGAAA GTCGAGTTTA CCACTCCCTA TCAGTGATAG AGAAAAGTGA AAGTCGAGTT TACCACTCCC TATCAGTGAT AGAGAAAAGT GAAAGTCGAG CTCGGTACCC GGGTCGAGGT AGGCGTGTAC GGTGGGAGGC CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCGCC TGGAGACGCC ATCCACGCTG TTTTGACCTC CATAGAAGAC ACCGGGACCG ATCCAGCCTC CGCGGCCCCG AATTCGAGCT CGGTACCCGG GGATCCGCCA CCATGGAGGC GGCGCGCACG GAGCGCCCCG CAGGCAGGCC GGGGGCGCCG CTTGTCCGGA CGGGGCTCCT ACTCTTGTCG ACGTGGGTCC TGGCCGGCGC CGAGATCACT TGGGACGCGA CAGGCGGTCC CGGACGCCCG GCGGCCCCGG CTTCGCGGCC ACCGGCGTTG TCTCCACTCT CGCCGCGGGC AGTGGCCAGC CAGTGGCCGG AGGAGCTGGC GTCGGCGCGG AGAGCCGCCG TGCTGGGGCG CCGGGCCGGA CCAGAGCTGC TGCCCCAGCA GGGCGGCGGC AGAGGCGGTG AGATGCAGGT GGAAGCCGGA GGGACATCAC CGGCAGGCGA GCGGCGGGGC CGGGGCATCC CAGCTCCTGC CAAGCTTGGC GGCGCGAGGA GGAGTCGCCG GGCGCAGCCC CCAATCACCC AGGAACGCGG GGACGCCTGG GCCACTGCTC CGGCCGATGG TTCCAGAGGA AGCCGTCCCC TTGCTAAGGG TTCCCGGGAG GAGGTGAAGG CGCCGCGGGC TGGGGGGTCG GCGGCTGAAG ACCTCCGGCT GCCCAGCACC TCCTTCGCGC TGACCGGGGA CTCGGCCCAC AACCAAGCCA TGGTGCACTG GTCGGGACAC AACAGCAGCG TCATACTTAT CCTGACGAAG CTGTATGACT TCAACCTGGG CAGCGTGACT GAGAGTTCAC TATGGAGGTC GACAGATTAT GGCACCACCT ATGAAAAGCT GAATGACAAA GTGGGTTTGA AGACTGTCCT CAGTTACCTC TATGTCAATC CAACCAACAA AAGGAAGATT ATGCTTCTCA GTGATCCTGA GATGGAGAGC AGCATATTGA TCAGCTCAGA CGAAGGGGCG ACCTATCAGA AGTATCGGCT CACCTTCTAT ATCCAGAGCC TGCTCTTTCA TCCCAAGCAA GAGGACTGGG TGCTGGCCTA CAGTTTGGAT CAAAAGCTCT ACAGCTCCAT GGACTTTGGA AGACGGTGGC AACTCATGCA TGAACGCATC ACACCCAACA GGTTTTATTG GTCGGTGGCC GGATTGGATA AGGAGGCGGA CCTGGTGCAC ATGGAGGTGC GGACCACGGA TGGATATGCT CACTACCTCA CCTGCAGGAT CCAGGAATGT GCCGAGACAA CTAGAAGTGG GCCTTTTGCC CGCTCCATTG ACATCAGTTC CCTGGTTGTC CAGGATGAAT ATATCTTCAT TCAGGTAACA ACTAGTGGAA GAGCCAGCTA CTACGTGTCT TATCGAAGAG AGGCCTTTGC TCAGATAAAG CTGCCTAAGT ACTCGTTGCC AAAGGACATG CACATCATCA GTACAGACGA GAACCAAGTA TTTGCTGCGG TCCAAGAATG GAACCAGAAC GACACGTACA ACCTCTACAT CTCAGACACG CGTGGGATTT ACTTCACTCT GGCCATGGAG AACATCAAGA GCAGCAGAGG TCTAATGGGG AACATCATTA TTGAATTGTA TGAGGTAGCA GGTATCAAAG GGATATTTCT GGCAAACAAG AAGGTGGACG ACCAGGTGAA GACATACATC ACTTACAACA AAGGCAGGGA TTGGCGCCTG CTGCAAGCTC CGGATGTGGA CCTGAGAGGA AGCCCAGTGC ACTGCCTGCT GCCCTTCTGT TCCTTACATC TGCACCTGCA ACTCTCTGAA AATCCATATT CCTCAGGAAG AATCTCTAGC AAGGAGACAG CCCCAGGACT TGTGGTGGCT ACAGGCAACA TTGGCCCGGA GCTCTCATAT ACTGATATTG GTGTGTTCAT CTCCTCCGAT GGGGGCAACA CATGGAGACA GATCTTTGAT GAAGAGTACA ATGTCTGGTT CCTAGACTGG GGTGGTGCCC TCGTGGCCAT GAAACACACA CCTCTGCCAG TCAGGCATTT GTGGGTGAGT TTTGATGAGG GCCACTCTTG GGACAAGTAT GGTTTCACTT CGGTTCCTCT CTTTGTTGAC GGGGCTCTGG TGGAGGCAGG AATGGAGACC CACATCATGA CAGTTTTTGG CCACTTCAGC CTCCGCTCCG AATGGCAATT GGTGAAAGTG GACTACAAAT CTATCTTCAG CCGGCATTGC ACCAAGGAGG ACTATCAGAC CTGGCACCTG CTCAATCAGG GAGAGCCTTG TGTCATGGGA GAAAGGAAAA TATTCAAGAA ACGTAAGCCA GGAGCTCAGT GTGCCCTGGG CCGAGACCAC TCAGGATCAG TGGTCTCAGA ACCCTGTGTC TGTGCCAATT GGGACTTCGA GTGTGACTAT GGGTATGAGA GACATGGGGA GAGCCAGTGT GTCCCAGCTT TCTGGTACAA TCCAGCATCC CCATCAAAGG ACTGCAGCCT TGGTCAAAGC TACCTTAACA GCACTGGGTA TCGGCGGATT GTGTCCAACA ACTGCACAGA TGGGCTAAGG GAGAAGTACA CCGCCAAGGC CCAGATGTGC CCTGGAAAAG CCCCTCGGGG CCTCCATGTG GTGACGACCG ATGGGCGGCT GGTGGCAGAG CAGGGGCACA ATGCAACTTT CATCATCCTC ATGGAGGAGG GTGATCTACA AAGGACAAAC ATCCAGCTTG ACTTTGGGGA TGGGATTGCT GTGTCCTACG CAAACTTCAG CCCCATCGAG GACGGCATCA AGCACGTGTA TAAGAGTGCG GGGATCTTCC AGGTGACAGC CTATGCAGAG AACAACCTTG GCTCAGACAC AGCTGTCCTC TTCCTGCATG TGGTTTGTCC TGTGGAGCAT GTTCATCTCC GAGTTCCATT TGTTGCCATA AGAAATAAGG AGGTCAACAT CAGTGCAGTC GTGTGGCCCA GTCAACTGGG GACCCTTACC TATTTCTGGT GGTTCGGCAA TAGCACAAAG CCTCTCATCA CTTTGGACAG CAGCATTTCC TTCACATTCC TTGCAGAAGG AACCGACACC ATCACAGTCC AGGTGGCTGC TGGGAATGCC CTCATCCAGG ACACAAAAGA GATTGCAGTT CATGAATATT TCCAGTCCCA GCTTTTATCA TTCTCTCCTA ATCTGGATTA CCACAATCCT GACATTCCTG AGTGGAGAAA AGATATTGGC AATGTCATCA AGCGAGCTCT GGTTAAAGTA ACCAGTGTCC CAGAGGACCA GATCCTCATT GCCGTGTTTC CTGGTCTCCC CACTTCAGCA GAGCTTTTCA TTCTTCCACC CAAGAACCTG ACAGAGAGGA GGAAAGGCAA TGAAGGGGAC CTGGAACAAA TTGTAGAAAC ACTGTTTAAT GCTCTCAACC AAAATTTGGT CCAGTTTGAG CTGAAGCCGG GGGTACAAGT CATTGTGTAT GTCACACAGC TGACGTTAGC TCCATTGGTG GACTCCAGTG CTGGGCACAG CAGCTCAGCC ATGCTTATGC TATTATCAGT GGTATTTGTT GGCCTGGCTG TGTTTTTGAT CTACAAGTTT AAAAGGAAAA TCCCTTGGAT TAACATCTAT GCTCAAGTCC AACACGACAA GGAGCAGGAG ATGATTGGGT CAGTGAGCCA AAGTGAAAAC GCCCCCAAAA TCACACTCAG TGACTTTACG GAGCCTGAGG AGCTGCTGGA CAAAGAGCTG GACACGCGGG TCATAGGAGG CATTGCCACT ATTGCAAACA GCGAAAGCAC AAAGGAGATC CCCAACTGCA CTAGTGTTTA AGCTAGCGCG GCCGCATCGA TAAGCTTGTC GACGATATCT CTAGAGCTGA GAACTTCAGG GTGAGTTTGG GGACCCTTGA TTGTTCTTTC TTTTTCGCTA TTGTAAAATT CATGTTATAT GGAGGGGGCA AAGTTTTCAG GGTGTTGTTT AGAATGGGAA GATGTCCCTT GTATCACCAT GGACCCTCAT GATAATTTTG TTTCTTTCAC TTTCTACTCT GTTGACAACC ATTGTCTCCT CTTATTTTCT TTTCATTTTC TGTAACTTTT TCGTTAAACT TTAGCTTGCA TTTGTAACGA ATTTTTAAAT TCACTTTTGT TTATTTGTCA GATTGTAAGT ACTTTCTCTA ATCACTTTTT TTTCAAGGCA ATCAGGGTAT ATTATATTGT ACTTCAGCAC AGTTTTAGAG AACAATTGTT ATAATTAAAT GATAAGGTAG AATATTTCTG CATATAAATT CTGGCTGGCG TGGAAATATT CTTATTGGTA GAAACAACTA CACCCTGGTC ATCATCCTGC CTTTCTCTTT ATGGTTACAA TGATATACAC TGTTTGAGAT GAGGATAAAA TACTCTGAGT CCAAACCGGG CCCCTCTGCT AACCATGTTC ATGCCTTCTT CTCTTTCCTA CAGCTCCTGG GCAACGTGCT GGTTGTTGTG CTGTCTCATC ATTTTGGCAA AGAATTCACT CCTCAGGTGC AGGCTGCCTA TCAGAAGGTG GTGGCTGGTG TGGCCAATGC CCTGGCTCAC AAATACCACT GAGATCTTTT TCCCTCTGCC AAAAATTATG GGGACATCAT GAAGCCCCTT GAGCATCTGA CTTCTGGCTA ATAAAGGAAA TTTATTTTCA TTGCAATAGT GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT GGGAGGGCAA ATCATTTAAA ACATCAGAAT GAGTATTTGG TTTAGAGTTT GGCAACATAT GCCCATATGC TGGCTGCCAT GAACAAAGGT TGGCTATAAA GAGGTCATCA GTATATGAAA CAGCCCCCTG CTGTCCATTC CTTATTCCAT AGAAAAGCCT TGACTTGAGG TTAGATTTTT TTTATATTTT GTTTTGTGTT ATTTTTTTCT TTAACATCCC TAAAATTTTC CTTACATGTT TTACTAGCCA GATTTTTCCT CCTCTCCTGA CTACTCCCAG TCATAGCTGT CCCTCTTCTC TTATGGAGAT CCCTCGACTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAC ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTTCACTC GAGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTTGC ATGCCTGCAG GTCGGCCGCC ACGACCGGTG CCGCCACCAT CCCCTGACCC ACGCCCCTGA CCCCTCACAA GGAGACGACC TTCCATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGACGCCCG CCCCACGACC CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGGCTC CGACCGAAGC CACCCGGGGC GGCCCCGCCG ACCCCGCACC CGCCCCCGAG GCCCACCGAC TCTAGAGGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGC
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