pUG34-SCEN0M00190(yeast)-mCherry
$99.00
In stock
SKU
PL42291
100μg liquid plasmid(Instantly):$120
| name | pUG34-SCEN0M00190(yeast)-mCherry | another name | apUG34-SCEN0M00190(yeast)-mCherry |
| Insert Size (bp) | 1761bp | Plasmid host | Yeast bacterium |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Yeast | Prokaryotic resistance | Amp |
| Selective marker | HIS3 | Fluorescent labeling | Red |
| Promoter | MET17 | Replicon | pUC,CEN/ARS |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGGACGG ATCGCTTGCC TGTAACTTAC ACGCGCCTCG TATCTTTTAA TGATGGAATA ATTTGGGAAT TTACTCTGTG TTTATTTATT TTTATGTTTT GTATTTGGAT TTTAGAAAGT AAATAAAGAA GGTAGAAGAG TTACGGAATG AAGAAAAAAA AATAAACAAA GGTTTAAAAA ATTTCAACAA AAAGCGTACT TTACATATAT ATTTATTAGA CAAGAAAAGC AGATTAAATA GATATACATT CGATTAACGA TAAGTAAAAT GTAAAATCAC AGGATTTTCG TGTGTGGTCT TCTACACAGA CAAGATGAAA CAATTCGGCA TTAATACCTG AGAGCAGGAA GAGCAAGATA AAAGGTAGTA TTTGTTGGCG ATCCCCCTAG AGTCTTTTAC ATCTTCGGAA AACAAAAACT ATTTTTTCTT TAATTTCTTT TTTTACTTTC TATTTTTAAT TTATATATTT ATATTAAAAA ATTTAAATTA TAATTATTTT TATAGCACGT GATGAAAAGG ACCCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTACCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC CTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCGGATGC AAGGGTTCGA ATCCCTTAGC TCTCATTATT TTTTGCTTTT TCTCTTGAGG TCACATGATC GCAAAATGGC AAATGGCACG TGAAGCTGTC GATATTGGGG AACTGTGGTG GTTGGCAAAT GACTAATTAA GTTAGTCAAG GCGCCATCCT CATGAAAACT GTGTAACATA ATAACCGAAG TGTCGAAAAG GTGGCACCTT GTCCAATTGA ACACGCTCGA TGAAAAAAAT AAGATATATA TAAGGTTAAG TAAAGCGTCT GTTAGAAAGG AAGTTTTTCC TTTTTCTTGC TCTCTTGTCT TTTCATCTAC TATTTCCTTC GTGTAATACA GGGTCGTCAG ATACATAGAT ACAATTCTAT TACCCCCATC CATACTCTAG AATGAGTTCC GTCAATAAAG ATACTATTCA TGTTGCTGAA AGAAGTCTTC ATAAAGAACA CCTTACCGAA GGTGGTAACA TGGCCTTCCA CAACCATTTG AATGATTTTG CTCATATTGA AGATCCTCTG GAAAGAAGAA GATTGGCTTT GGAGTCCATC GATGACGAAG GTTTCGGTTG GCAACAAGTT AAGACCATCT CCATTGCTGG TGTTGGTTTC TTGACAGATT CTTATGATAT TTTTGCCATT AATTTGGGTA TCACTATGAT GTCCTACGTT TACTGGCACG GTAGTATGCC AGGTCCAAGT CAAACCTTGT TGAAGGTTTC CACTTCTGTT GGTACTGTTA TTGGTCAATT TGGTTTTGGT ACTTTAGCTG ATATTGTTGG TCGTAAGAGA ATTTATGGTA TGGAACTTAT TATCATGATT GTCTGTACCA TTCTGCAAAC CACTGTTGCT CATTCTCCTG CTATTAACTT CGTTGCTGTT TTAACATTCT ACCGTATTGT CATGGGTATT GGTATCGGTG GTGACTACCC ACTATCTTCT ATTATTACTT CTGAATTTGC CACTACCAAA TGGAGAGGTG CCATCATGGG TGCTGTCTTT GCTAACCAAG CTTGGGGTCA AATCTCCGGT GGTATCATCG CTCTTATCTT GGTTGCTGCT TACAAGGGCG AACTAGAATA CGCAAACTCT GGTGCTGAAT GTGATGCTAG ATGTCAAAAG GCTTGTGACC AAATGTGGAG AATCCTTATT GGGTTGGGTA CCGTTCTAGG GTTGGCATGT TTGTATTTCA GATTAACTAT TCCAGAATCT CCTAGATATC AATTGGATGT TAACGCTAAG TTGGAACTTG CTGCTGCCGC ACAAGAACAA GATGGCGAAA AGAAAATTCA CGACACCAGT GATGAAGACA TGGCAATTAA CGGTTTGGAA AGAGCTTCTA CTGCCGTCGA ATCTCTTGAC AATCATCCTC CAAAGGCTTC GTTCAAAGAT TTCTGCAGAC ATTTTGGTCA ATGGAAGTAC GGTAAGATTT TGCTAGGTAC TGCTGGTTCA TGGTTTACCT TAGATGTTGC TTTCTACGGG TTGAGTTTAA ACAGTGCTGT TATTCTGCAA ACCATCGGTT ATGCCGGTTC CAAAAACGTT TACAAGAAAC TGTATGATAC TGCTGTCGGT AATCTGATTT TGATTTGTGC TGGTTCATTA CCTGGTTACT GGGTATCCGT CTTCACTGTC GATATAATCG GTAGAAAACC AATTCAATTA GCCGGTTTCA TCATCTTGAC CGCTTTGTTC TGTGTCATCG GTTTCGCATA CCATAAACTT GGTGACCATG GTCTGTTGGC TCTTTACGTC ATTTGTCAAT TCTTCCAAAA CTTCGGTCCA AACACAACCA CCTTTATTGT TCCTGGTGAG TGTTTCCCAA CTCGTTACAG ATCTACTGCT CATGGTATTT CTGCTGCATC TGGTAAGGTC GGTGCCATTA TTGCACAAAC CGCTTTGGGT ACTCTAATCG ACCATAACTG TGCTAGAGAC GGTAAGCCAA CCAACTGTTG GTTACCTCAC GTCATGGAAA TTTTCGCCTT ATTCATGTTG TTGGGTATCT TCACAACCTT GTTGATCCCA GAAACTAAGA GAAAGACTCT AGAAGAAATT AACGAGCTAT ACCACGATGA AATCGATCCT GCTACGCTAA ACTTCAGAAA CAAGAATAAT GACATTGAAT CTTCCAGCCC ATCTCAACTT CAACATGAAG CATCTAGAAC TAGTGGATCC ATGGTGAGCA AGGGCGAGGA GGATAACATG GCCATCATCA AGGAGTTCAT GCGCTTCAAG GTGCACATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGTGG CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCTCAGTTC ATGTACGGCT CCAAGGCCTA CGTGAAGCAC CCCGCCGACA TCCCCGACTA CTTGAAGCTG TCCTTCCCCG AGGGCTTCAA GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC CCTGCAGGAC GGCGAGTTCA TCTACAAGGT GAAGCTGCGC GGCACCAACT TCCCCTCCGA CGGCCCCGTA ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCTCCGAGC GGATGTACCC CGAGGACGGC GCCCTGAAGG GCGAGATCAA GCAGAGGCTG AAGCTGAAGG ACGGCGGCCA CTACGACGCT GAGGTCAAGA CCACCTACAA GGCCAAGAAG CCCGTGCAGC TGCCCGGCGC CTACAACGTC AACATCAAGT TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGAA CGCGCCGAGG GCCGCCACTC CACCGGCGGC ATGGACGAGC TGTACAAGTA AGAATTCGAT ATCAAGCTTA TCGATACCGT CGACCTCGAG TCATGTAATT AGTTATGTCA CGCTTACATT CACGCCCTCC CCCCACATCC GCTCTAACCG AAAAGGAAGG AGTTAGACAA CCTGAAGTCT AGGTCCCTAT TTATTTTTTT ATAGTTATGT TAGTATTAAG AACGTTATTT ATATTTCAAA TTTTTCTTTT TTTTCTGTAC AGACGCGTGT ACGCATGTAA CATTATACTG AAAACCTTGC TTGAGAAGGT TTTGGGACGC TCGAAGGCTT TAATTTGCGG CCGGTACCCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GTTTACAATT TCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATAGATCCGT CGAGTTCAAG AGAAAAAAAA AGAAAAAGCA AAAAGAAAAA AGGAAAGCGC GCCTCGTTCA GAATGACACG TATAGAATGA TGCATTACCT TGTCATCTTC AGTATCATAC TGTTCGTATA CATACTTACT GACATTCATA GGTATACATA TATACACATG TATATATATC GTATGCTGCA GCTTTAAATA ATCGGTGTCA CTACATAAGA ACACCTTTGG TGGAGGGAAC ATCGTTGGTA CCATTGGGCG AGGTGGCTTC TCTTATGGCA ACCGCAAGAG CCTTGAACGC ACTCTCACTA CGGTGATGAT CATTCTTGCC TCGCAGACAA TCAACGTGGA GGGTAATTCT GCTAGCCTCT GCAAAGCTTT CAAGAAAATG CGGGATCATC TCGCAAGAGA GATCTCCTAC TTTCTCCCTT TGCAAACCAA GTTCGACAAC TGCGTACGGC CTGTTCGAAA GATCTACCAC CGCTCTGGAA AGTGCCTCAT CCAAAGGCGC AAATCCTGAT CCAAACCTTT TTACTCCACG CGCCAGTAGG GCCTCTTTAA AAGCTTGACC GAGAGCAATC CCGCAGTCTT CAGTGGTGTG ATGGTCGTCT ATGTGTAAGT CACCAATGCA CTCAACGATT AGCGACCAGC CGGAATGCTT GGCCAGAGCA TGTATCATAT GGTCCAGAAA CCCTATACCT GTGTGGACGT TAATCACTTG CGATTGTGTG GCCTGTTCTG CTACTGCTTC TGCCTCTTTT TCTGGGAAGA TCGAGTGCTC TATCGCTAGG GGACCACCCT TTAAAGAGAT CGCAATCTGA ATCTTGGTTT CATTTGTAAT ACGCTTTACT AGGGCTTTCT GCTCTGTCAT CTTTGCCTTC GTTTATCTTG CCTGCTCATT TTTTAGTATA TTCTTCGAAG AAATCACATT ACTTTATATA ATGTATAATT CATTATGTGA TAATGCCAAT CGCTAAGAAA AAAAAAGAGT CATCCGCTAG GGGAAAAAAA AAAATGAAAA TCATTACCGA GGCATAAAAA AATATAGAGT GTACTAGAGG AGGCCAAGAG TAATAGAAAA AGAAAATTGC GGGAAAGGAC TGTGTTATGA CTTCCCTGAC TAATGCCGTG TTCAAACGAT ACCTGGCAGT GACTCCTAGC GCTCACCAAG CTCTTAAAAC GGGAATTTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACGCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGA
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