pDHtsk-GFP-G418
$113.00
In stock
SKU
PL42214
100μg liquid plasmid(Instantly):$134
| name | pDHtsk-GFP-G418 | another name | apDHtsk-GFP-G418 |
| Insert Size (bp) | Plasmid host | filiformFungus | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | gpdA | Replicon | pVS1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACATTAACG TTTACAATTT CGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAGCGCGCG TAATACGACT CACTATAGGG CGAATTGGGT ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CCTGCAGCCC GGGGGATCCG CGCAATTAAC CCTCACTAAA GGGAACAAAA GCTGGAGCTC CACCGCGGTG GCGGCCGCGA CGTTAACTGA TATTGAAGGA GCACTTTTTG GGCTTGGCTG GAGCTAGTGG AGGTCAACAA TGAATGCCTA TTTTGGTTTA GTCGTCCAGG CGGTGAGCAC AAAATTTGTG TCGTTTGACA AGATGGTTCA TTTAGGCAAC TGGTCAGATC AGCCCCACTT GTAGCAGTAG CGGCGGCGCT CGAAGTGTGA CTCTTATTAG CAGACAGGAA CTAGGACATT ATCATCATCT GCTGCTTGGT GCACGATAAC TTGGTGCGTT TGTCAAGCAA GGTAAGTGAA CGACCCGGTC ATACCTTCTT AAGGGGTTTC TGGAGTTTAA TGAGCTAAGC ACATACGTCA GAAACCATTA TTGCGCGTTC AAAAGTCGCC TAAGGTCACT ATCAGCTAGC AAATATTTCT TGTCAAAAAT GCTCCACTGA CGTTCCATAA ATTCCCCTCG GTATCCAATT AGAGTCTCAT ATTCACTCTC AATCCAAATA ATCTGCACCG GATCTGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GATGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACGGGATCTC TGCGGAACAG GCGGTCGAAG GTGCCGATAT CATTACGACA GCAACGGCCG ACAAGCACAA CGCCACGATC CTGAGCGACA ATATGATCGG GCCCGGCGTC CACATCAACG GCGTCGGCGG CGACTGCCCA GGCAAGACCG AGATGCACCG CGATATCTTG CTGCGTTCGG ATATTTTCGT GGAGTTCCCG CCACAGACCC GGATGATCCC CGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCACGCGT TTTCGGGTTT ACCTCTTCCA GATACAGCTC AACTGCAATG CATTAATGCA TTGGACCTCG CAACCCTAGT ACGCCCTTCA GGCTCCGGCG AAGCAGAAGA ATAGCTTAGC AGAGTCTATT TTCATTTTCG GGAGACGAGA TCAAGCAGAT CAACGGTCGT CAAGAGACCT ACGAGACTGA GGAATCCGCT CTTGGCTCCA CGCGACTATA TATTTGTCTC TAATTGTACT TTGACATGCT CCTCTTCTTT ACTCTGATAG CTTGACTATG AAAATTCCGT CACCAGCCCT GACTAGTGCA TTGCAGATGA GCTGTATCTG GAAGAGGTAA ACCCGAAACG CGTTTTATTC TTGTTGACAT GGAGCTATTA AATCACTAGA AGGCACTCTT TGCTGCTTGG ACAAATGAAC GTATCTTATC GAGATCCTGA ACACCATTTG TCTCAACTCC GGAGCTGACA TCGACACCAA CGATCTTATA TCCAGATTCG TCAAGCTGTT TGATGATTTC AGTAACGTTA AGTGGATCTC GGTGACGGGC AGGACCGGAC GGGGCGGTAC CGGGCCCCCC CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TACTTGTATA GTTCATCCAT GCCATGTGTA ATCCCAGCAG CTGTTACAAA CTCAAGAAGG ACCATGTGGT CTCTCTTTTC GTTGGGATCT TTCGAAAGGG CAGATTGTGT GGACAGGTAA TGGTTGTCTG GTAAAAGGAC AGGGCCATCG CCAATTGGAG TATTTTGTTG ATAATGGTCT GCTAGTTGAA CGCTTCCATC TTCAATGTTG TGTCTAATTT TGAAGTTAAC TTTGATTCCA TTCTTTTGTT TGTCTGCCAT GATGTATACA TTGTGTGAGT TATAGTTGTA TTCCAATTTG TGTCCAAGAA TGTTTCCATC TTCTTTAAAA TCAATACCTT TTAACTCGAT TCTATTAACA AGGGTATCAC CTTCAAACTT GACTTCAGCA CGTGTCTTGT AGTTCCCGTC ATCTTTGAAA AATATAGTTC TTTCCTGTAC ATAACCTTCG GGCATGGCAC TCTTGAAAAA GTCATGCTGT TTCATATGAT CTGGGTATCT CGCAAAGCAT TGAACACCAT AACCGAAAGT AGTGACAAGT GTTGGCCATG GAACAGGTAG TTTTCCAGTA GTGCAAATAA ATTTAAGGGT AAGTTTTCCG TATGTTGCAT CACCTTCACC CTCTCCACTG ACAGAAAATT TGTGCCCATT AACATCACCA TCTAATTCAA CAAGAATTGG GACAACTCCA GTGAAAAGTT CTTCTCCTTT ACTCATGGAT CCTCTAGAGT CGAGGTCGAC GGTATCGATA AGCTTGATTA AAGGTTCTTG GATGGGAAGA TGAATATACT GAAGATGGGA AAAGAAAGAG AAAAGAAAAG AGCAGCTGGT GGGGAGAGCA GGAAAATATG GCAACAAATG TTGGACTGAC GCAACGACCT TGTCAACCCC GCCGACACAC CGGGCGGACA GACGGGGCAA AGCTGCCTAC CAGGGACTGA GGGACCTCAG CAGGTCGAGT GCAGAGCACC GGATGGGTCG ACTGCCAGCT TGTGTTCCCG GTCTGCGCCG CTGGCCAGCT CCTGAGCGGC CTTTCCGGTT TCATACACCG GGCAAAGCAG GAGAGGCACG ATATTTGGAC GCCCTACAGA TGCCGGATGG GCCAATTAGG GAGCTTACGC GCCGGGTACT CGCTCTACCT ACTTCGGAGA AGGTACTATC TCGTGAATCT TTTACCAGAT CGGAAGCAAT TGGACTTCTG TACCTAGGTT AATGGCATGC TATTTCGCCG ACGGCTATAC ACCCCTGGCT TCACATTCTC CTTCGCTTAC TGCCGGTGAT TCGATGAAGC TCCATATTCT CCGATGATGC AATAGATTCT TGGTCAACGA GGGGCACACC AGCCTTTCCA CTTCGGGGCG GAGGGGCGGC CGGTCCCGGA TTAATAATCA TCCACTGCAC CTCAGAGCCG CCAGAGCTGT CTGGCGCAGT GGCGCTTATT ACTCAGCCCT TCTCTCTGCG TCCGTCCGTC TCTCCGCATG CTCTAGAGCG GCCGCCACCG CGGTGGAGCT CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTCCGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGhC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGA AGGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGCTAGAGCA GCTTGAGCTT GGATCAGATT GTCGTTTCCC GCCTTCAGTT TAAACTATCA GTGTTTGACA GGATATATTG GCGGGTAAAC CTAAGAGAAA AGAGCGTTTA TTAGAATAAC GGATATTTAA AAGGGCGTGA AAAGGTTTAT CCGTTCGTCC ATTTGTATGT GCATGCCAAC CACAGGGTTC CCCTCGGGAT CAAAGTACTT TGATCCAACC CCTCCGCTGC TATAGTGCAG TCGGCTTCTG ACGTTCAGTG CAGCCGTCTT CTGAAAACGA CATGTCGCAC AAGTCCTAAG TTACGCGACA GGCTGCCGCC CTGCCCTTTT CCTGGCGTTT TCTTGTCGCG TGTTTTAGTC GCATAAAGTA GAATACTTGC GACTAGAACC GGAGACATTA CGCCATGAAC AAGAGCGCCG CCGCTGGCCT GCTGGGCTAT GCCCGCGTCA GCACCGACGA CCAGGACTTG ACCAACCAAC GGGCCGAACT GCACGCGGCC GGCTGCACCA AGCTGTTTTC CGAGAAGATC ACCGGCACCA GGCGCGACCG CCCGGAGCTG GCCAGGATGC TTGACCACCT ACGCCCTGGC GACGTTGTGA CAGTGACCAG GCTAGACCGC CTGGCCCGCA GCACCCGCGA CCTACTGGAC ATTGCCGAGC GCATCCAGGA GGCCGGCGCG GGCCTGCGTA GCCTGGCAGA GCCGTGGGCC GACACCACCA CGCCGGCCGG CCGCATGGTG TTGACCGTGT TCGCCGGCAT TGCCGAGTTC GAGCGTTCCC TAATCATCGA CCGCACCCGG AGCGGGCGCG AGGCCGCCAA GGCCCGAGGC GTGAAGTTTG GCCCCCGCCC TACCCTCACC CCGGCACAGA TCGCGCACGC CCGCGAGCTG ATCGACCAGG AAGGCCGCAC CGTGAAAGAG GCGGCTGCAC TGCTTGGCGT GCATCGCTCG ACCCTGTACC GCGCACTTGA GCGCAGCGAG GAAGTGACGC CCACCGAGGC CAGGCGGCGC GGTGCCTTCC GTGAGGACGC ATTGACCGAG GCCGACGCCC TGGCGGCCGC CGAGAATGAA CGCCAAGAGG AACAAGCATG AAACCGCACC AGGACGGCCA GGACGAACCG TTTTTCATTA CCGAAGAGAT CGAGGCGGAG ATGATCGCGG CCGGGTACGT GTTCGAGCCG CCCGCGCACG TCTCAACCGT GCGGCTGCAT GAAATCCTGG CCGGTTTGTC TGATGCCAAG CTGGCGGCCT GGCCGGCCAG CTTGGCCGCT GAAGAAACCG AGCGCCGCCG TCTAAAAAGG TGATGTGTAT TTGAGTAAAA CAGCTTGCGT CATGCGGTCG CTGCGTATAT GATGCGATGA GTAAATAAAC AAATACGCAA GGGGAACGCA TGAAGGTTAT CGCTGTACTT AACCAGAAAG GCGGGTCAGG CAAGACGACC ATCGCAACCC ATCTAGCCCG CGCCCTGCAA CTCGCCGGGG CCGATGTTCT GTTAGTCGAT TCCGATCCCC AGGGCAGTGC CCGCGATTGG GCGGCCGTGC GGGAAGATCA ACCGCTAACC GTTGTCGGCA TCGACCGCCC GACGATTGAC CGCGACGTGA AGGCCATCGG CCGGCGCGAC TTCGTAGTGA TCGACGGAGC GCCCCAGGCG GCGGACTTGG CTGTGTCCGC GATCAAGGCA GCCGACTTCG TGCTGATTCC GGTGCAGCCA AGCCCTTACG ACATATGGGC CACCGCCGAC CTGGTGGAGC TGGTTAAGCA GCGCATTGAG GTCACGGATG GAAGGCTACA AGCGGCCTTT GTCGTGTCGC GGGCGATCAA AGGCACGCGC ATCGGCGGTG AGGTTGCCGA GGCGCTGGCC GGGTACGAGC TGCCCATTCT TGAGTCCCGT ATCACGCAGC GCGTGAGCTA CCCAGGCACT GCCGCCGCCG GCACAACCGT TCTTGAATCA GAACCCGAGG GCGACGCTGC CCGCGAGGTC CAGGCGCTGG CCGCTGAAAT TAAATCAAAA CTCATTTGAG TTAATGAGGT AAAGAGAAAA TGAGCAAAAG CACAAACACG CTAAGTGCCG GCCGTCCGAG CGCACGCAGC AGCAAGGCTG CAACGTTGGC CAGCCTGGCA GACACGCCAG CCATGAAGCG GGTCAACTTT CAGTTGCCGG CGGAGGATCA CACCAAGCTG AAGATGTACG CGGTACGCCA AGGCAAGACC ATTACCGAGC TGCTATCTGA ATACATCGCG CAGCTACCAG AGTAAATGAG CAAATGAATA AATGAGTAGA TGAATTTTAG CGGCTAAAGG AGGCGGCATG GAAAATCAAG AACAACCAGG CACCGACGCC GTGGAATGCC CCATGTGTGG AGGAACGGGC GGTTGGCCAG GCGTAAGCGG CTGGGTTGTC TGCCGGCCCT GCAATGGCAC TGGAACCCCC AAGCCCGAGG AATCGGCGTG ACGGTCGCAA ACCATCCGGC CCGGTACAAA TCGGCGCGGC GCTGGGTGAT GACCTGGTGG AGAAGTTGAA GGCCGCGCAG GCCGCCCAGC GGCAACGCAT CGAGGCAGAA GCACGCCCCG GTGAATCGTG GCAAGCGGCC GCTGATCGAA TCCGCAAAGA ATCCCGGCAA CCGCCGGCAG CCGGTGCGCC GTCGATTAGG AAGCCGCCCA AGGGCGACGA GCAACCAGAT TTTTTCGTTC CGATGCTCTA TGACGTGGGC ACCCGCGATA GTCGCAGCAT CATGGACGTG GCCGTTTTCC GTCTGTCGAA GCGTGACCGA CGAGCTGGCG AGGTGATCCG CTACGAGCTT CCAGACGGGC ACGTAGAGGT TTCCGCAGGG CCGGCCGGCA TGGCCAGTGT GTGGGATTAC GACCTGGTAC TGATGGCGGT TTCCCATCTA ACCGAATCCA TGAACCGATA CCGGGAAGGG AAGGGAGACA AGCCCGGCCG CGTGTTCCGT CCACACGTTG CGGACGTACT CAAGTTCTGC CGGCGAGCCG ATGGCGGAAA GCAGAAAGAC GACCTGGTAG AAACCTGCAT TCGGTTAAAC ACCACGCACG TTGCCATGCA GCGTACGAAG AAGGCCAAGA ACGGCCGCCT GGTGACGGTA TCCGAGGGTG AAGCCTTGAT TAGCCGCTAC AAGATCGTAA AGAGCGAAAC CGGGCGGCCG GAGTACATCG AGATCGAGCT AGCTGATTGG ATGTACCGCG AGATCACAGA AGGCAAGAAC CCGGACGTGC TGACGGTTCA CCCCGATTAC TTTTTGATCG ATCCCGGCAT CGGCCGTTTT CTCTACCGCC TGGCACGCCG CGCCGCAGGC AAGGCAGAAG CCAGATGGTT GTTCAAGACG ATCTACGAAC GCAGTGGCAG CGCCGGAGAG TTCAAGAAGT TCTGTTTCAC CGTGCGCAAG CTGATCGGGT CAAATGACCT GCCGGAGTAC GATTTGAAGG AGGAGGCGGG GCAGGCTGGC CCGATCCTAG TCATGCGCTA CCGCAACCTG ATCGAGGGCG AAGCATCCGC CGGTTCCTAA TGTACGGAGC AGATGCTAGG GCAAATTGCC CTAGCAGGGG AAAAAGGTCG AAAAGGTCTC TTTCCTGTGG ATAGCACGTA CATTGGGAAC CCAAAGCCGT ACATTGGGAA CCGGAACCCG TACATTGGGA ACCCAAAGCC GTACATTGGG AACCGGTCAC ACATGTAAGT GACTGATATA AAAGAGAAAA AAGGCGATTT TTCCGCCTAA AACTCTTTAA AACTTATTAA AACTCTTAAA ACCCGCCTGG CCTGTGCATA ACTGTCTGGC CAGCGCACAG CCGAAGAGCT GCAAAAAGCG CCTACCCTTC GGTCGCTGCG CTCCCTACGC CCCGCCGCTT CGCGTCGGCC TATCGCGGCC GCTGGCCGCT CAAAAATGGC TGGCCTACGG CCAGGCAATC TACCAGGGCG CGGACAAGCC GCGCCGTCGC CACTCGACCG CCGGCGCCCA CATCAAGGCA CCCTGCCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA TAGCGGAGTG TATACTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGCATTCTA GGTACTAAAA CAATTCATCC AGTAAAATAT AATATTTTAT TTTCTCCCAA TCAGGCTTGA TCCCCAGTAA GTCAAAAAAT AGCTCGACAT ACTGTTCTTC CCCGATATCC TCCCTGATCG ACCGGACGCA GAAGGCAATG TCATACCACT TGTCCGCCCT GCCGCTTCTC CCAAGATCAA TAAAGCCACT TACTTTGCCA TCTTTCACAA AGATGTTGCT GTCTCCCAGG TCGCCGTGGG AAAAGACAAG TTCCTCTTCG GGCTTTTCCG TCTTTAAAAA ATCATACAGC TCGCGCGGAT CTTTAAATGG AGTGTCTTCT TCCCAGTTTT CGCAATCCAC ATCGGCCAGA TCGTTATTCA GTAAGTAATC CAATTCGGCT AAGCGGCTGT CTAAGCTATT CGTATAGGGA CAATCCGATA TGTCGATGGA GTGAAAGAGC CTGATGCACT CCGCATACAG CTCGATAATC TTTTCAGGGC TTTGTTCATC TTCATACTCT TCCGAGCAAA GGACGCCATC GGCCTCACTC ATGAGCAGAT TGCTCCAGCC ATCATGCCGT TCAAAGTGCA GGACCTTTGG AACAGGCAGC TTTCCTTCCA GCCATAGCAT CATGTCCTTT TCCCGTTCCA CATCATAGGT GGTCCCTTTA TACCGGCTGT CCGTCATTTT TAAATATAGG TTTTCATTTT CTCCCACCAG CTTATATACC TTAGCAGGAG ACATTCCTTC CGTATCTTTT ACGCAGCGGT ATTTTTCGAT CAGTTTTTTC AATTCCGGTG ATATTCTCAT TTTAGCCATT TATTATTTCC TTCCTCTTTT CTACAGTATT TAAAGATACC CCAAGAAGCT AATTATAACA AGACGAACTC CAATTCACTG TTCCTTGCAT TCTAAAACCT TAAATACCAG AAAACAGCTT TTTCAAAGTT GTTTTCAAAG TTGGCGTATA ACATAGTATC GACGGAGCCG ATTTTGAAAC CGCGGTGATC ACAGGCAGCA ACGCTCTGTC ATCGTTACAA TCAACATGCT ACCCTCCGCG AGATCATCCG TGTTTCAAAC CCGGCAGCTT AGTTGCCGTT CTTCCGAATA GCATCGGTAA CATGAGCAAA GTCTGCCGCC TTACAACGGC TCTCCCGCTG ACGCCGTCCC GGACTGATGG GCTGCCTGTA TCGAGTGGTG ATTTTGTGCC GAGCTGCCGG TCGGGGAGCT GTTGGCTGGC TGGTGGCAGG ATATATTGTG GTGTAAACAA ATTGACGCTT AGACAACTTA ATAACACATT GCGGACGTTT TTAATGTACT GAATTAACGC CGAATTAATT CGGGGGATCT GGATTTTAGT ACTGGATTTT GGTTTTAGGA ATTAGAAATT TTATTGATAG AAGTATTTTA CAAATACAAA TACATACTAA GGGTTTCTTA TATGCTCAAC ACATGAGCGA AACCCTATAG GAACCCTAAT TCCCTTATCT GGGAACTACT CACACATTAT TATGGAGAAA CTTGGCAA
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