pPDGFRA(human)-mut2-Fluc-hRluc-Neo
$70.00
In stock
SKU
PL42181
100μg liquid plasmid(Instantly):$91
| name | pPDGFRA(human)-mut2-Fluc-hRluc-Neo | another name | apPDGFRA(human)-mut2-Fluc-hRluc-Neo |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | Rluc,Fluc |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TATAGTCCGG AAATACAGGA ACGCACGCTG GATGGCCCTT CGCTGGGATG GTGAAACCAT GAAAAATGGC AGCTTCAGTG GATTAAGTGG GGGTAATGTG GCCTGTACCC TCTGGTTGCA TAGGTATTCA TACGGTTAAA ATTTATCAGG CGCGATTGCG GCAGTTTTTC GGGTGGTTTG TTGCCATTTT TACCTGTCTG CTGCCGTGAT CGCGCTGAAC GCGTTTTAGC GGTGCGTACA ATTAAGGGAT TATGGTAAAT CCACTTACTG TCTGCCCTCG TAGCCATCGA GATAAACCGC AGTACTCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGAGCTC AAGCTTCGAA TTCATAACAA AGGCATCATT CAAATTAGGC AGGTAATTAC TACCAGAAGG ACAACTGGGT GCTCGCTTGC TCATCCATTG CTTTCTGCTT TAAACTCCCG AGGAGTCGCT GCCTGGAGAC ACTCCCGCCC GGACTGCTTC TCGGGCCCCA TTAAACGGCT CTGAAATGAA CTGTGAAGTT ACCATTTGTG GGCGGAGAGC GCTGGGGCCC TGCGGACGGA AGACCCTGGC TTGACCGGAC TAACAGTGTC GCCGAAGCAA AGCAGCCCCT CTGGGTAGGC TGTCTGTCTC GCCTCCTACC CTCTGGGGCA ACTAGCCTAA AAACCCGGTT CTCAACTTAA CAGCATGTGG CCCAGCACCC GCTTTTGCCT TTTATTCCGC ACTCTGATTT TTGGGGGGAT CGTTTGTTCC CGCTCATTTT CTACAAACAT TACGGTATCC TGTTAGCATT CCGAACAAGG GGCTGTTCAT ATATTTGCCT TCAATGATTT CCTGAAGCTT CCACATGTCC TAATAGCGAG CGGTGCAGTC ACCCAAGCTG AAGATGCACG AGAGCGGGTC GCGTAGAAGA ACTGCGGCAA TGGGACCCCC AGCGCAGCCC CAGGCGCCCT GTTTTCGAGG CTGACATCCC AAGATCCTGT CCTGGTTAGG CACAGCACCG CGCCCGTTGG AGTACTTCTC TGTGACTCTC CCCTGTCCAC TGCACTGTGT CCACCCTCCT GGTGAAGGAG GAGACGGCTG TTGGACATGC CAGTCCCAAC CTGGCCACTT TGGCCATTTT CAGAGTGCGT GTGTTGGGGT GGGGTTGAGG GAGAAGGATG AAGGATGACC CCCAACCCCA ACAGGAGAAT TCAAAAATCA GAGAGCGATG AAGGTGAACG CGCTCCCCTC CCCCGCTGTC GCTGGGAAGC CCCACTCTGC CCAGGTCTGG TTCCTGGAGC ATCAGCGCCC CCTTTCCCTC GCCGGCAGGA TCGTTTCCTC CCGACACTGG CCTCTCGCCT TGGGCGCAGG GTTTGGGGCT CAGCTGTCAA TTCCAAGGAA TTCGTAGGGT TCGACCCACG CGGCGGGAGG GAACGGAGGG AGAATCAAAA GGCATCCTCC CCCACGACGC GGAGGCGGTG GTACGATTCC TCAAACGCCC AAGCTTGAGC TTTTTTTGTA TGCGAGATAG AAGCCAGGGC AACCTCGCCC AGATAACCCC GAAAACAAAG GCACGACCAG ATAAGTGACT TCAAGGGAGT AAAGGTCGTT CTATCGGAGA ACGTTCCAAA CCTACACAGA CCCTCTTTTC TTTGTACCGT ATTTCAGACC ACCCAGTCTT GTACACACAC ACACACCACA CAAAACAAAA ACCCATCATT GTCATATTGG ACTCAACAGT TTGCCCAATC CTATTAAATA ACTAGCAACT ATATAGCCCT TCCCCTAAAA GACCCCTAGT TCAAAACGGC GCAACCGCTT GGAGATTTCT CCCGAGGGCC CTATTTCTCG TTGGGCCGAG TTTGTAGAAG GGACATTTCT TGATTCTAGA TTTATATTCT CTCTGGGTAT TAAATGCAAT TTTGTATGTT CCTTTTCTTC TTTTCAAAGA GAATACAAAA CTATAGCAGT CTAAGAAAAT AACCCCAAAA TGGGGAGGCA AACTCATTCT GGAAATGATG GGCTGTTTGT AGTTTCACAA AACCTCTTTC CCGGCAGAGC ACCAACACCT CCCCCTTCCA CCACCCCCCA TCCCATCTGG TCTGCTTCTC CCCGCCCCCC AGTTGTTGTC GAAGTCTGGG GGTTGGGACT GGACCCCCTG ATTGCGTAAG AGCAAAAAGC GAAGGCGCAA TCTGGACACT GGGAGATTCG GAGCGCAGGG AGTTTGAGAG AAACTTTTAT TTTGAAGAGA CCAAGGTTGA GGGGGGGCTT ATTTCCTGAC AGCTATTTAC TTAGAGCAAA TGATTAGTTT TAGAAGGATG GACTATAACA TTGAATCAAT TACAAAACGC GGTTTTTGAG CCCATTACTG TTGGAGCTAC AGGGAGAGAA ACAGAGGAGG AGACTGCAAG AGATCATTGG AGGCCGTGGG CACGCTCTTT ACTCCATGTG TGGGACATTC ATTGGATCCC ACCATGGAAG ATGCCAAAAA CATTAAGAAG GGCCCAGCGC CATTCTACCC ACTCGAAGAC GGGACCGCCG GCGAGCAGCT GCACAAAGCC ATGAAGCGCT ACGCCCTGGT GCCCGGCACC ATCGCCTTTA CCGACGCACA TATCGAGGTG GACATTACCT ACGCCGAGTA CTTCGAGATG AGCGTTCGGC TGGCAGAAGC TATGAAGCGC TATGGGCTGA ATACAAACCA TCGGATCGTG GTGTGCAGCG AGAATAGCTT GCAGTTCTTC ATGCCCGTGT TGGGTGCCCT GTTCATCGGT GTGGCTGTGG CCCCAGCTAA CGACATCTAC AACGAGCGCG AGCTGCTGAA CAGCATGGGC ATCAGCCAGC CCACCGTCGT ATTCGTGAGC AAGAAAGGGC TGCAAAAGAT CCTCAACGTG CAAAAGAAGC TACCGATCAT ACAAAAGATC ATCATCATGG ATAGCAAGAC CGACTACCAG GGCTTCCAAA GCATGTACAC CTTCGTGACT TCCCATTTGC CACCCGGCTT CAACGAGTAC GACTTCGTGC CCGAGAGCTT CGACCGGGAC AAAACCATCG CCCTGATCAT GAACAGTAGT GGCAGTACCG GATTGCCCAA GGGCGTAGCC CTACCGCACC GCACCGCTTG TGTCCGATTC AGTCATGCCC GCGACCCCAT CTTCGGCAAC CAGATCATCC CCGACACCGC TATCCTCAGC GTGGTGCCAT TTCACCACGG CTTCGGCATG TTCACCACGC TGGGCTACTT GATCTGCGGC TTTCGGGTCG TGCTCATGTA CCGCTTCGAG GAGGAGCTAT TCTTGCGCAG CTTGCAAGAC TATAAGATTC AATCTGCCCT GCTGGTGCCC ACACTATTTA GCTTCTTCGC TAAGAGCACT CTCATCGACA AGTACGACCT AAGCAACTTG CACGAGATCG CCAGCGGCGG GGCGCCGCTC AGCAAGGAGG TAGGTGAGGC CGTGGCCAAA CGCTTCCACC TACCAGGCAT CCGCCAGGGC TACGGCCTGA CAGAAACAAC CAGCGCCATT CTGATCACCC CCGAAGGGGA CGACAAGCCT GGCGCAGTAG GCAAGGTGGT GCCCTTCTTC GAGGCTAAGG TGGTGGACTT GGACACCGGT AAGACACTGG GTGTGAACCA GCGCGGCGAG CTGTGCGTCC GTGGCCCCAT GATCATGAGC GGCTACGTTA ACAACCCCGA GGCTACAAAC GCTCTCATCG ACAAGGACGG CTGGCTGCAC AGCGGCGACA TCGCCTACTG GGACGAGGAC GAGCACTTCT TCATCGTGGA CCGGCTGAAG AGCCTGATCA AATACAAGGG CTACCAGGTA GCCCCAGCCG AACTGGAGAG CATCCTGCTG CAACACCCCA ACATCTTCGA CGCCGGGGTC GCCGGCCTGC CCGACGACGA TGCCGGCGAG CTGCCCGCCG CAGTCGTCGT GCTGGAACAC GGTAAAACCA TGACCGAGAA GGAGATCGTG GACTATGTGG CCAGCCAGGT TACAACCGCC AAGAAGCTGC GCGGTGGTGT TGTGTTCGTG GACGAGGTGC CTAAAGGACT GACCGGCAAG TTGGACGCCC GCAAGATCCG CGAGATTCTC ATTAAGGCCA AGAAGGGCGG CAAGATCGCC GTGTAATTCT AGTTGTTTAA ACGAGCTCGC TAGCCTCGAG TCTAGAGTCG ACCTGCAGGC ATGCAAGCTG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCGGCCGCTT CGAGCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG ATGTGGGAGG TTTTTTTAAG CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CGAATTTTAA CAAAATATTA ACGCTTACAA TTTCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATACGCGG ATCTGCGCAG CACCATGGCC TGAAATAACC TCTGAAAGAG GAACTTGGTT AGGTACCTTC TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTTGATTCTT CTGACACAAC AGTCTCGAAC CAAAGGCTGG AGCCACCATG GCTTCCAAGG TGTACGACCC CGAGCAACGC AAACGCATGA TCACTGGGCC TCAGTGGTGG GCTCGCTGCA AGCAAATGAA CGTGCTGGAC TCCTTCATCA ACTACTATGA TTCCGAGAAG CACGCCGAGA ACGCCGTGAT TTTTCTGCAT GGTAACGCTG CCTCCAGCTA CCTGTGGAGG CACGTCGTGC CTCACATCGA GCCCGTGGCT AGATGCATCA TCCCTGATCT GATCGGAATG GGTAAGTCCG GCAAGAGCGG GAATGGCTCA TATCGCCTCC TGGATCACTA CAAGTACCTC ACCGCTTGGT TCGAGCTGCT GAACCTTCCA AAGAAAATCA TCTTTGTGGG CCACGACTGG GGGGCTTGTC TGGCCTTTCA CTACTCCTAC GAGCACCAAG ACAAGATCAA GGCCATCGTC CATGCTGAGA GTGTCGTGGA CGTGATCGAG TCCTGGGACG AGTGGCCTGA CATCGAGGAG GATATCGCCC TGATCAAGAG CGAAGAGGGC GAGAAAATGG TGCTTGAGAA TAACTTCTTC GTCGAGACCA TGCTCCCAAG CAAGATCATG CGGAAACTGG AGCCTGAGGA GTTCGCTGCC TACCTGGAGC CATTCAAGGA GAAGGGCGAG GTTAGACGGC CTACCCTCTC CTGGCCTCGC GAGATCCCTC TCGTTAAGGG AGGCAAGCCC GACGTCGTCC AGATTGTCCG CAACTACAAC GCCTACCTTC GGGCCAGCGA CGATCTGCCT AAGATGTTCA TCGAGTCCGA CCCTGGGTTC TTTTCCAACG CTATTGTCGA GGGAGCTAAG AAGTTCCCTA ACACCGAGTT CGTGAAGGTG AAGGGCCTCC ACTTCAGCCA GGAGGACGCT CCAGATGAAA TGGGTAAGTA CATCAAGAGC TTCGTGGAGC GCGTGCTGAA GAACGAGCAG ACCGGTGGTG GGAGCGGAGG TGGCGGATCA GGTGGCGGAG GCTCCGGAGG GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAT GGCCGCAATA AAATATCTTT ATTTTCATTA CATCTGTGTG TTGGTTTTTT GTGTGAATCG ATAGCGATAA TCTTTGCGCT TGCGTTTTCC CTTGTCCAGA TAGCCCAGTA GCTGACATTC ATCCGGGGTC AGCACCGTTT CTGCGGACTG GCTTTCTACG TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTCA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACTATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA TTCGAAATGA CCGACCAAGC GACGCCCAAC CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TTTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAA
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